MicrobeHunter.com Microscopy Forum

apochronaut wrote: ↑

Mon Jul 13, 2020 11:23 am

Is there no where that you can try these models out?

I wish!

I used a 50/50 trinocular split for quite a long time and found it worked very well. Liked being able to see through the eyepieces while photographing. I would say that unless you are going to use very light intensive techniques such as high magnification Darkfield you will be fine. I suppose it de...

Hi all I recently started a thread about possibly getting a new scope. One that I'm looking at is the https://moticeurope.com/en/panthera-cc-trinocular.html . This series, though, has a fixed 50:50 trinocular split. I'm trying to get my head around whether this could be a deal breaker. It has illumi...

...I could probably do with a better camera. The one I have is ok-ish (It's a Touptek 5M with Aptina sensor and 2.2um pixels) but tends to be a bit grainy. If the camera is an eyepiece camera, so is being used without eyepiece, the Nikon CF objective is expected to perform very well, since it is in...

Maybe I would be better off just getting a new scope complete with, hopefully, better objectives, after all? It is true that the objectives are the heart of a microscope, but you do have to place them in a 'good' stand. The one you have is not going to do them justice. If you want to get some nikon...

...Using the 20x, doing the projection on to a piece of paper gives a changing circle of light which comes into focus at a point above the head - I'm guessing at ~160mm! Same with 10x finite objective. So I really need better quality finite objectives from somewhere... Although the reproducibility ...

Might one of these be better than I have: https://www.ebay.co.uk/itm/Nikon-Microscope-Objective-lens-CF-CFN-Plan-20-0-50/293463180519?hash=item4453c4c4e7:g:59EAAOSw-s5ePDkq Or would there actually be little in it? I read a post on here from 2016 ("Better objectives for a Chinese microscope") where t...

...At the end of the day, there is really just the objective lens and the camera, from an imaging point of view. It ain't necessarily so. On the BX50 imaging system showed above, I believe that it was the objective, tube lens and camera; if one had used the dedicated Olympus camera like the DP70, D...

In any case, comparing the finite and 'infinity' objectives by using them as magnifying glasses doesn't show any obvious differences ... No need for a magnifying glass, just turn on your microscope as you would normally do, focus on a slide, then take off the head and place a paper over the light b...

75RR, thanks for the idea. Done. Hi Um, the particular page isn't viewable. In any case, comparing the finite and 'infinity' objectives by using them as magnifying glasses doesn't show any obvious differences plus I'm obviously getting an image without any kind of tube lens. It's possible that it's...

That's a pretty dismal image. Tons of spherical aberration and increasing off axis ca.....especially for a 20X of only .4 N.A., which should have some resistance to spherical aberration due to the low N.A. Reports of people using infinity objectives in finite systems successfully and visa versa are...

FWIW Here's a brightfield image of some pollen taken with the objective in question:
I wish I had another instrument to compare but it's similar to images tagen with the x40, just smaller, of course!

Louise

I don't think it is supposed to work. This is what microscopyu says: https://www.microscopyu.com/microscopy-basics/microscope-objective-specifications Most manufacturers have now transitioned to infinity-corrected objectives that project emerging rays in parallel bundles from every azimuth to infin...

75RR wrote: ↑

Thu Jul 09, 2020 5:34 pm

What is the Numerical Aperture of the 20x objective?
[/quote]

It's 0.40

Louise

I was just wondering about the use of infinity-corrected objectives in a finite tube? My old Brunel (above) has a 160mm tube length. I bought a x20 plan objective for it a while ago (all 4 are Plan now) but the one I received is marked as an infinity objective. However, it seems to work ok (or am I ...

Hi David I thought I'd replied to this earlier but maybe I didn't 'submit', d'uh. Anyway, I'd asked you what Motic objectives you have/had? Are they 'EC' Plan achromats? They do 10x/22mm UC eyepieces and UC Plan objectives (optional Phase) for the Panthera. I asked Motic what the difference is. The...

ps it seems to be sat in my Outbox. How long before I'm able to actually reply to a PM?? [/quote] When you send a PM it will remain in your Outbox until read by the recipient. That's standard behavior for this forum software. [/quote] It was said above that, as a new member, I can't yet reply to PM...

I had a Brunel microscope with plan objectives and decided to upgrade to a Motic BA310 I found that the change to 30mm eyepieces was a huge improvement in viewing comfort, but the Motic ojectives were no better than the generic Brunel objectives and in a direct comparison actually show more chromat...

Have sent PM, but not sure if you have been a member long enough. Here is reply I sent: "Thanks for your message. I'm in Scotland but not mobile. I'd like to use the phase contrast for looking at live cells. It would be a nice to have. The Panthera can be fitted with a phase contrast turret that al...

Here is an example of an Olympus BH-2. With phase contrast. Just picked it out of eBay. I am not related to the seller in any way. https://www.ebay.com/itm/Olympus-Trinocular-BH2-BHTU-Phase-Contrast-Microscope/203040702828?hash=item2f462b416c:g:IMwAAOSwpq1fAnd7 With phase condenser and two SPlans (...

Have sent PM, but not sure if you have been a member long enough. New members can receive but not sent PMs so you would need to include a means of contact. I did send a reply - at least, it gave me the appearance of being sent! Louise ps it seems to be sat in my Outbox. How long before I'm able to ...

75RR wrote: ↑

Wed Jul 08, 2020 3:15 pm

photomicro wrote: ↑

Wed Jul 08, 2020 1:03 pm

Have sent PM, but not sure if you have been a member long enough.

New members can receive but not sent PMs so you would need to include a means of contact.

I did send a reply - at least, it gave me the appearance of being sent!
Louise

That would suggest that new Euromex or Motic are poor quality? I am saying that the quality of the big 4 research microscopes from the 160 tube length era is superior. Still, a used microscope is not for everyone. You should however look into it before deciding. Have a read through this pdf: http:/...

"£2000 is a very generous budget within the used microscope market, and I mean very generous! You can get a good quality used microscope with phase for less than half that with a little patience." That would suggest that new Euromex or Motic are poor quality? My current one was relatively cheap but ...

Hi Here is a pic of my current scope: MicroscopeSmall.jpg I'm in the UK. I think I bought it from Brunel Microscopes. It was in storage for quite a few years. I've only recently resurrected my interest though I've previously used research grade microscopes at Uni. The big 4 are way too expensive for...

Thanks for the replies. It looks like I'll just have to go through all the specs/features of the Panthera vs Oxion and do a detailed compare. Then make a decision...

Louise

Hi I just joined this forum :) I've had an oldish unbranded trinocular scope since the '90s which I've upgraded to plan objectives. However, I'd like to get a better, newer trinocular scope and one already set up for phase contrast. I've been looking at the Euromex Oxion model and the Motic Panthera...