MicrobeHunter.com Microscopy Forum

Louise, That is what I think. I run very hot water on it before each use and that about takes care of it. -Dennis No, it does not. To pasteurize you need at least 10 minutes at a minimum of 240 ° Fahrenheit. Your immune system is stronger and more capable than you might expect. That's why you never...

Louise, That is what I think. I run very hot water on it before each use and that about takes care of it. -Dennis Good! I've not had any upset stomach or gastro-intestinal problem in many, many years :) But, also, I don't eat out or ever get takeaways - just my own home cooked food. Works for me! :...

Just to say there's a difference between bacteria. generally, and 'germs'. Bacteria are ubiquitous and the vast majority are not pathogenic. Indeed, our own gut flora are essential to our health though need to have the right balance via a healthy diet. I use one of those yellow and green sponges to ...

I wasn't complaining. It just takes an hour or two. I can do other things while it uploads. My speed may be a little slow but I'm uploading 2 or 3 gigabytes each time. Hi Don Hmm... It's up to you what sizes and dimensions of videos you choose to upload. I recently uploaded a non-microscopy video w...

MickyFynn wrote: ↑

Thu Sep 22, 2022 4:18 pm

LouiseScot wrote: ↑

Thu Sep 22, 2022 4:15 pm

MickyFynn wrote: ↑

Thu Sep 22, 2022 4:03 pm

My reply was aimed at Don as it was he who complained about the time it took!
Louise

Louise...I'm very new to this..please accept my apologies..

It's ok!

I upload a lot on You Tube. It sometimes takes 3 or 4 hours to upload a full video. You must have a slow internet connection, Don! What upload speed do you have? Mine is 10Mbs but it doesn't take too long (minutes rather than hours) to upload a typical video, and I'm in the UK. It's best to keep th...

I upload a lot on You Tube. It sometimes takes 3 or 4 hours to upload a full video. You must have a slow internet connection, Don! What upload speed do you have? Mine is 10Mbs but it doesn't take too long (minutes rather than hours) to upload a typical video, and I'm in the UK. It's best to keep th...

I don't think you can upload anything but small images here, upload on YT or other external service... thanks for that..what's YT? YouTube - upload video to there and insert a link in a post on here. Similarly, you can also upload larger stills to your hosting site of choice and insert a link on he...

In case it is far from parfocal you use the objective with the wrong distance to the object. In some cases not much happens, in other cases the image suffers a lot. Hi I did some fine tuning earlier to make sure the camera sensor is as parfocal with the oculars as possible. It was very close anyway...

MicroBob wrote: ↑

Sun Sep 18, 2022 1:10 am

Is your camera parfocal with the eyepieces?

Almost! Though I fine focus the camera image via the laptop screen anyway I'll be having a closer look at things today.

Louise

It's interesting that the violet color stood oit to you but not the equal and opposite green also present in tbe imagr. There might still be a screen issue of some sort that's making that color more apparent. Ah, there was more green too, originally, but that lessened after I did the custom white b...

imkap wrote: ↑

Sat Sep 17, 2022 9:21 pm

LouiseScot wrote: ↑

Sat Sep 17, 2022 9:15 pm

Hiya
The LED illumination certainly could be a factor. But I still ....

My camera too registers more CA than I do. Try fiddling with white balance a bit.

Hiya

Um, see my original post!

Louise

Here is an example. The same diatom was imaged with a plain (non phase contrast) 40X0.75 Neofluar objective. Afocal photography, my previous 6500K LED light, filtered through a KR12 amber filter (my present light is 4000K, high CR LED). One image is brightfield, the other oblique. Both images were ...

I believe it is the LED light. Occasionally I observed my diatom slides under halogen light, and there were no purple fringes. Under my current LED, there are fringes, especially under oblique light. I will try and post an example. But, diatoms are not the best demonstrators of CA. The diatom frust...

I don't see a violet tinge, just typical axial CA. Could it be that your monitor has gotten off calibration? Hi Um what is 'axial CA' ? It's what you're seeing. There are two kinds of chromatic aberration, color separation horizontally (lateral ca) and color separation axially (ie towards or away f...

Thats interesting, I thought direct projection largely eliminates CA, not sure about axial CA tho. You can eliminate the magenta and greens with photo editing software but I suspect you want to eliminate it at the source. Yeah, I wasn't expecting CA with a hq plan fluor but the fact that I don't se...

Wes wrote: ↑

Sat Sep 17, 2022 4:52 pm

What projection method do you use? Afocal or direct projection?

It's an infinity microscope so direct projection after the tube lens. I'm not sure whether there are any additional optics after the tube lens. The view through the oculars doesn't show any tinge.

Louise

I don't see a violet tinge, just typical axial CA. Could it be that your monitor has gotten off calibration? Hi Um what is 'axial CA' ? It's actually not an all-over tinge - just in certain places like the flat area in the centre of the central diatom. It seems to be independent of the monitor. Her...

I have to admit I'm out of practice with photomicrography, but I was trying out a 40x/0.85 inf pan fluor lens on the Amscope T720 using a diatom strew. It's set up to use my Canon 1100d (Rebel T3) for imaging. Everything is ok except that my images seem to be coming out with a violet/purple colour c...

Olympus, Nikon and Zeiss each have their own resources plus other places online and in forums etc. e.g. https://www.olympus-lifescience.com/en/ ... iguration/

Louise

A Toupcam type usb 3 eyepiece camera http://www.touptek.com/product/showproduct.php?lang=en&id=78 is lensless and goes in place of an ocular. You connect to a laptop/pc and view images on the larger screen so you get to check the image/video and the focus ahead of taking a picture or recording a vi...

A Toupcam type usb 3 eyepiece camera http://www.touptek.com/product/showproduct.php?lang=en&id=78 is lensless and goes in place of an ocular. You connect to a laptop/pc and view images on the larger screen so you get to check the image/video and the focus ahead of taking a picture or recording a vid...

It looks like it's a reduced Go-Pro-type wide angle 'action camera'. You might be better off with a dedicated EP microscope camera e.g. Toupcam

Louise

Louise, I dint believe you will need a secondary tube. My epi scope has only one. Greg Oh ok - thanks. I was a little unsure as infinity optics always confuse me! At the moment I'm working towards an image of the LED to be focused on the objective BFP and the field iris image to be projected on to ...

Thanks, louise for alerting us to your upcoming DIC project. I too will ( within a few weeks I guess) drag out my cobeled setup..and target cheek cells for an attempt at 'poor mans transmitted DIC'. I eagerly await your set up and findings, louise! best to all, charlie g. I'm working on making an e...

Hi Charlie I was reading your posts with interest. I had a go setting up DIC with a Nikon Optiphot nosepiece incorporating prisms earlier in the year. I tried to match the nosepiece up with a Zeiss DIC condenser but couldn't get it to work properly. I got a bit distracted and then put it all to one ...

would work ok with an adjustable trinocular Indeed, Nikon's photo tube is 3 pieces screwed together; unscrewing the top 2 would make the focal plane below photo relays available for direct projection to mirrorless camera sensors. Sadly, Nikon applied some sort of cement to those threads that I have...

direct projection to a camera Direct projection wants a camera sensor is at a focal plane, which, by design, is below the first lens in oculars or photo relays. Refocusing objectives to increase their tube length 40mm or so exacerbates aberrations. I just took these otherwise unedited 1:1 crops fro...

23.2mm for an EP normally refers to the OD for the adapter to fit into the microscope standard EP tube. e.g. this add shows a 30mm one and 23.2mm one side by side https://www.ebay.co.uk/itm/140938461878 I have seen those, but never understood their purpose; what optic fits inside such a tube with 2...

The one in your ad can't be both C-mount and 23.2mm for an EP and od of 30mm I found an old JVC video camera with C-mount; it does fit, and the rest now also fits 23.2mm eyepieces. Even before shimming, 22mm of the tube had a smaller i.d. than that final C-mount threaded 6mm, just not small enough....