MicrobeHunter.com Microscopy Forum

Thanks, Stomias . But could you specify - does the adapter contribute to it or this aberration is caused only by the objective ?

The the black lines on the ruler images of LouiseScot have blurry sides with green/blue contours, like they are illuminated sidewise https://i.ibb.co/mFc63XH/question1.jpg Could anyone please tell where do these artifacts come from? I'm using identical DSLR adapter, and I find these effects very ann...

Hello! I have recently purchased a used Olympus OM-D EM-5 camera and attempted to use it with my microscope. I got an inexpensive 2X DSLR camera adapter from e-bay for mounting this camera on the eyepiece tube and c-mount phototube. Much to my regret this adapter causes glistering/flare/fleck (sorry...

Thank you for you kind reply, PeteM ! Yes, I'm working in a research institute, but I have produced these images for my personal study. The histology is not a part of my job, but rather a hobby, and I have purchased the microtome and microscope for my entertainment at home.

The higher power view of ventricular and subventricular zones where the radial glial cells actively divide (60x objective) : https://i.ibb.co/pnpJF8D/60x-forebrain-VZ-EDITED.jpg The intermediate zone filled with migratory neurons that possess oval or irregulary shaped nuclei, imaged with 60x objecti...

The subventricular, intermediate zones and cortical plate (20x objective): https://i.ibb.co/RyjTVbn/20x-forebrain-edited.jpg The ventricular/subventricular and intermediate zones imaged with 40x objective: https://i.ibb.co/nc1tYT9/40x-forebrain-VZ-IZ-edited.jpg The intermediate zone (40x objective):...

Images show histological section of embryonic mouse forebrain stained with diaminobenzidine (brown) and hematoxylin (purple). Brown color shows nestin staining, a protein that is only present in progenitors of neurons. Nestin is found in bodies of these cells and their long processes, spanning the w...

Thank you for your replies, everyone! BramHuntingNematodes, yes of course the mouse is okay. It juts took a little time off and departed for awhile :) tgss, the lack of our understanding about this structure appears surprising to me too. However, when we start digging in any area of the modern scien...

Thank you, tgss! You are right, the subcomissural organ is the central item on these images. As it's a very distinctive feature in brain, which can be easily identified, I used it for comparison of section morphology. This organ is believed to regulate the flow of cerebrospinal liquid through brain ...

This is my home-made brain section stained with three different dyes. Green staining for neurons, magenta staining for astrocytes and blue staining for cell nuclei. Scale bar 50 micron. Images were resized and cropped to achieve equal size. Top row - chinese LF-302 microscope, 40x plan achromat Midd...

I have made several sections of mouse brain of different thickness which were mounted on the same slide and stained simultaneously with hematoxylin-eosin. Thicker 5 micron ones adsorb more dyes and show brighter staining. Thinner three micron sections attain only faint colors, but have crisper detai...

hans, thank you for the detailed explanation! The rail grease is behind the trick, I may take advantage of that.

Are you sure there is anything holding them down other than the retaining screw at the end which keeps them from sliding out axially I still haven't checked this, I'm scared to be left with no stage as the reassembly may be not trivial with my skills. I want to complete a couple of projects and gat...

Thank you so much for your support hans , BramHuntingNematodes and especially microscoperecovery ! There may be a solution to this problem without switching the stage Thank you for the advice, most probably I will try to purchase the rods soon and try to replace them. In the past people here often s...

Thank you, apochronaut ! Now I'm feeling more confident that I can demand a free stage replacement. It was a big mistake to forget stating "I'm not using overweight protists in my experiments" in the complaining mail to the supplier :D When you say that the mount is usually more substantial, you mea...

Hello! I'm sorry to bother you again with this silly topic, but I have finally discovered the root of my problem Please see the image of the X-axis rail. I was blind by not seeing that there are severe defects in the mechanism. https://i.ibb.co/9Z7YmRP/Stage-defect.jpg I have contacted the supplier ...

I've noticed that the slide holder movement is less stiff compared to my previous microscope, and it appeared that the holder arms too weakly adjoin to the table. This was observed even before I have polished the holder's bottom surface. I attempted to bend the holder arm downwards, while controllin...

Thank you for sharing your experience! It is a very interesting report. I feel that I did well going this route rather than a new Chinese 'scope. The more I read here, the more convinced I become that old microscopes from quality producers are much better than shiny new ones from China. Alas I have ...

Sorry for the late response, I was very badly ill during the last week. I have investigated the X-axis stage movement mechanism but found no apparent irregularities in it. There is no decent point where the focus gets lost, the problem is observed along the whole length of the slide. However the sta...

Hello again! Please help me to understand another annoying feature of my microscope. The focus is lost at some point when I move the stage along the X-axis looking for example at the stage micrometer . When I return the stage to the initial position, the image becomes sharp again. Thus, I really dou...

The best software for Image analysis is a free NCBI program ImageJ. Even Zeiss Zen Blue is inferior, let alone the others.

The camera is mounted this way: https://i.yapx.ru/H755Ks.jpg The male screw facing us serves to fix the the camera rotation. The bottom male screws (barely seen) are needed to mount the adapter and center it against the phototube The camera C-mount thread is directly installed on the adapter, there ...

By the way, I was using for the long time the color 9MP MU900 Aptina CMOS camera for USB2.0 bought from the Amscope. In fact this is UCMOS09000KPB camera made by ToupTek, China, (as well as all other cameras sold by Amscope). The Amscope may not confirm that information. But you can easily reveal th...

I would suggest to try to get the camera parfocal and test the image quality again I would gladly do that, but my camera adapter has no special mechanism for adjustment of camera focus/height My impression is that it even improves the image quality when it shouldn't show Unfortunately, I experience...

Good day, Bob! Is it possible that you expect too much of your achromat? This is quite possible! I will surely look to get an apochromat objective in future. Please tell, will the Plan Fluorite objectives also show better sharpness in the edges of the FOV? I intended to use my 10x Plan achromat for ...

I wish to express my gratitude again to all who have spared their precious time for my question! MicroBob , you say: - Did you actually turn the objective or did you turn the object micrometer?- I did the first - rotated the objective in its nosepiece thread . Thank you, I understand the consequence...

Thank you for your replies MichaelG. and Hobbyst46 ! I would probably go further, and replace the word ‘chinese’ with ‘typical’ Did you also have the unsatisfactory results with other inexpensive Plan achro objectives? Did they have a similar problem? And could you please explain what do you mean by...

MicroBob , thank you for the advice! I think I'm making some progress now. I have employed the stage micrometer for this experiment. I only have the old russian one, it is only 1 mm in length. It does not cover the whole field of view but it still useful to estimate that off-focus areas. As I rotat...

Thank you for your replies! I see you both disregard the chinese optics. I knew there must have been some catch behind the low price. My previous microscope was Amscope T340B, also produced in china. It had N-117M number on it, which can be easily tracked to Xiamen Phio Scientific Instruments. Since...

Good day! Please enlighten me if there could be some spherical aberration observed on the the field of view edges when using the 10x Plan achromat objective (Considering that the condernser aperture is set to a proper NA and the field diaphragm is open no more than it is neccessary to illuminate the...