MicrobeHunter.com Microscopy Forum

Indeed it is

In great condition, comes with original box and 2x lens.
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These came with my microscope, but I've never had cause to use them. I figure someone else could get better use out of them. The objectives are Epiplan 2.5 0.08 Epiplan HD 6.3 0.16 Epiplan HD 16 0.32 Epiplan HD 40 0.65 The turret is in good working order. The diaphragm still operates very smoothly. ...

These came with my microscope, but I've never had cause to use them. I figure someone else could get better use out of them. The objectives are Epiplan 2.5 0.08 Epiplan HD 6.3 0.16 Epiplan HD 16 0.32 Epiplan HD 40 0.65 The turret is in good working order. The diaphragm still operates very smoothly. ...

BramHuntingNematodes wrote: ↑

Mon Aug 09, 2021 3:26 am

Have you been oiling it to the bottom of your slide?

(That's a very nice df condenser)

Not exactly...

By that I mean 'no'. Okay, I'll have a play with that and see what happens, thanks.

Markus

I should also mention I've been achieving a 'kind of dark-field' by deliberately using the wrong annulus on the phase contrast condenser, but I have a nagging feeling that a proper darkfield condenser might do better?

Hey all, I bought a secondhand Zeiss Universal a while back and a bunch of other stuff came in the box too, including this darkfield condenser. Maybe I'm an idiot, but I can't get it to work, don't know if it *should work, or if it even works with my system (the scope came with a bunch of other stuf...

Wow - that's amazing!

Since the thin line is so thin, likely even better resolution is possible, but to prove that, one needs narrower spaces between distinct objects. Indeed, the stage micrometer is not the appropriate indicator of microscope resolution (even for low-mag objectives). So I guess the appropriate method w...

I don't know if this helps. This is an uncropped, but scaled image of the entire frame with measurements (again, 100x objective, 2x optivar and 12.5x EP - only using OIL this time). The objective is a Neofluar PH3 130 Oel 160/- The insert image is at 100% and unscaled. So the blue line represents th...

Very happy with that … but I think Markus has made a ‘leap of faith’ in assuming that he therefore has 0.52µm resolution I see nothing in the posted photo to demonstrate resolution of two points separated by half a micron. [ and nor would I expect to ] MichaelG. Sorry, maybe I wasn't clear. I come ...

I think what I'm trying to do is to be able to estimate sizes by eyeballing the EP graticule, because the scale on the slide won't be there when looking at specimens, and it will be quicker than photographing and measuring in photoshop. So if I can use a scale of known dimensions to define the lengt...

The larger image has been cropped, and the inset is just a picture taken using my smartphone at the eyepiece to capture the EP graticule so you can see it overlaid on the slide graticule. But IIRC, the FoV in the eyepiece was larger than the camera. I didnt think the cropping would matter as I'm try...

Okay, in this example, the scope is maxed out. EP's at 12.5x, Objective at 100x and the Optovar in the middle is at 2x. Altogether that's a total magnification of 12.5 * 2 * 100 = 2500 I'm not using oil, even though it's an oil objective. The scale on the slide says that each division is .01mm There...

Hey All, I was just calibrating my microscope with a calibration slide and realised that at the highest magnification (2500 x) that one unit on the eyepiece graticule is equal to 0.5 um (or 500 nanometers). Unless I've done my sums wrong, that is the wavelength of visible light somewhere between the...

Thanks guys, awesome, my first amoeba! Always wanted to find one! Stoked!!

And thanks for the website recommendation. Has anyone combined all these individual microbe identification websites into one master list? Usually they specialise in a single kind of micronial life.

I'm kind of new to this, but I'm a bit stumped.

That's the closest I can find in my book.

Video Here

https://youtu.be/Wv4bYVmJ1Eg

Markus

Hi all, I'm thinking of making some of those slides that use petroleum jelly to seal the edge so they don't dry out so fast. Thing is, I've also noticed that I need to leave the lid off the pond water sample if I don't want it to go anaerobic. Currently there's quite a nice little ecosystem potterin...

The Zeiss Universal uses chromatic corrections in the eyepieces so a Zeiss West eyepiece or projective should be part of the adaptation. There are two ways: 1.) Using a high eyepoint eyepiece (Brille) together with a pancake camera objective as Wes describes. 2.) Using a projective to project the i...

MicroBob wrote: ↑

Fri Mar 26, 2021 8:05 am

Does you photo tube contain a projective? If it fits for the C35 it would fit for a full frame digital camera too.

yes, it has a small eyepiece inside the photo tube that presumably projects the image to the camera (a 6.3x IIRC).

Best,

Markus

MicroBob wrote: ↑

Fri Mar 26, 2021 8:05 am

Hi Markus,
if you post a picture we will know what you have there.

Bob

Thanks Bob, I had the picture all ready and forgot to attach it.

I've added it to the original post in an edit.

Cheers

Markus

Hi all; This came with the scope - part number 47 30 23 - 9900. It has an eyepiece inside for projection onto the focal plane and the bottom tube telescopes inside the top one - I assume so it can be made to be parfocal with the visual eyepieces? Anyway it comes with an old Zeiss C35 camera setup wh...

40X not 40mm. A 40mm objective would be about 2X. Actually 250/40 = 6.25x, I think :) I have 25mm Astro EP's as oculars. They give a max possible fn and a magnification of 250/25 = 10x, plus good eye relief :) Edit: Thinking about it, the above only applies to oculars. If you use actual tube length...

40X not 40mm. A 40mm objective would be about 2X. I used to own one of those Leitz 40X .95 with a correction collar. I was able to do a direct comparison of it with very similar Spencer and Bausch & Lomb apochromats, a 44X .95 with correction collar and a 45X .95 with correction collar. Of the 3, t...

Actually as a strange adjunct to this, I just tested my neofluar 40mm/0.75 against a friend's 40mm/0.95 apochromat in a Leitz microscope from the 50's (can't remember the exact model) and unexpectedly the Neofluar was better. Not sure why this should be. I did adjust the collar for the coverslips I'...

If your objectives are Neofluars (regardless of phase contrast or no phase contrast), they are NOT achromats. Neofluars are made of a specific type of glass and are designed (and do) to give better optical corrections - both chromatic and axial - than achromats. Ah, that's good to know. I had read ...

You can get pretty sharp with a plain old achromat in certain conditions, particularly if you are OK with monochrome illumination. Nice idea, I'll have to try this once my new illuminator turns up. there is already a green filter just below the condensor - I assume that is what this is for. It woul...

Fourth, the lower priced objectives are also often not compatible with DIC. Olympus specs SPlan (likely with some fluorite glass given the N.A. and with wider fields) for finite DIC. Their achromats don't work, the DPlans are marginal, the Plan Apos work. Similarly with Leica's and Nikon's lineup -...