Search found 12 matches

by highbeta
Tue Nov 03, 2020 4:48 pm
Forum: Illumination Techniques
Topic: Problems with flourescence picture on ZEISS Axiovert 25
Replies: 10
Views: 1001

Re: Problems with flourescence picture on ZEISS Axiovert 25

I received the EX485/20X excitation filter. By looking without any fluorescent specimen the image of the lamp disappeared but I still saw blue background. So I ordered a new beam splitter 500 DCLP and an emission filter 515 EFLP. Will keep you posted hopefully with nice pictures soon. thanks, highbeta
by highbeta
Mon Nov 02, 2020 4:52 pm
Forum: Illumination Techniques
Topic: Problems with flourescence picture on ZEISS Axiovert 25
Replies: 10
Views: 1001

Re: Problems with flourescence picture on ZEISS Axiovert 25

I was able to find a Chroma ET485/20x FITC Excitation Filter Ring Mounted CWL 485 nm BW 20 nm D 25mm for a decent price. As soon as I get it and test it, I will post the results.

Thanks for the the hint with the Omega shop.

highbeta
by highbeta
Mon Nov 02, 2020 4:36 pm
Forum: Camera systems and imaging
Topic: Canon M50 to Zeiss Axiovert 25
Replies: 4
Views: 568

Re: Canon M50 to Zeiss Axiovert 25

Thanks for you response, doing some batch post processing should not be a big deal. Since an overlay of brightfield and flourence pictures will be done anyway. I will have to work on the overall workflow anyway. Just out of curiosity, the optimal magnification for an APS-C sensor would be 1.6x, corr...
by highbeta
Sun Nov 01, 2020 7:57 am
Forum: Camera systems and imaging
Topic: Canon M50 to Zeiss Axiovert 25
Replies: 4
Views: 568

Canon M50 to Zeiss Axiovert 25

I need some help to adapt a Canon EOS M50 (APS-C sensor with 22.3 x 14.9 mm) to an Zeiss Axiovert 25 CFL Microscope. A Zeiss V25 1.0x C-Mount video adapter (1108-963) came already with the microscope. Adapting the camera with a simple EF-M to C-mount adapter works not great because the poor sensor u...
by highbeta
Tue Oct 27, 2020 6:02 am
Forum: Illumination Techniques
Topic: Problems with flourescence picture on ZEISS Axiovert 25
Replies: 10
Views: 1001

Re: Problems with flourescence picture on ZEISS Axiovert 25

Thanks a lot for that information. I think have to bite the bullet and invest into a new filter or even a set to be sure that everything fits together. The current filters came together with the microscope. I do not know, if this is still the original setup from 2002 or was replaced at some point. T...
by highbeta
Mon Oct 26, 2020 3:34 pm
Forum: Illumination Techniques
Topic: Problems with flourescence picture on ZEISS Axiovert 25
Replies: 10
Views: 1001

Problems with flourescence picture on ZEISS Axiovert 25

All, I have problems with a fluorescence microscope (Zeiss Axiovert 25 CFL) looking at GFP fluorescing cells. I see a lot of background light and can even see the mercury lamp arcs. Please see attached pictures, unfortunately the background noise isn't captured good by the camera, but it is there wh...
by highbeta
Mon Oct 12, 2020 2:05 am
Forum: Microscopes and optics
Topic: Zeiss Axiovert 25 CFL vs. Axiovert 40 CFL
Replies: 0
Views: 366

Zeiss Axiovert 25 CFL vs. Axiovert 40 CFL

All, I am looking for a fluorescence microscope to be used for cell culture. I identified the ZEISS Axiovert microscopes as a good workhorse. Does someone have information about the difference between the Axiovert 25 CFL and a Axiovert 40 CFL? From pictures I do not see any significant difference, b...
by highbeta
Sat Oct 10, 2020 8:34 am
Forum: Beginner's corner
Topic: Olympus CK Inverted Microscope Upgrade
Replies: 17
Views: 6436

Re: Olympus CK Inverted Microscope Upgrade

Hi All,

I wonder if one of you guys can help me with a manual for an Olympus CK/CKP/CKC?
viewtopic.php?f=15&t=10480

Thanks in advance,
Highbeta
by highbeta
Wed Sep 30, 2020 1:59 pm
Forum: Resources (online, books etc.)
Topic: Looking for a Olympus Tokyo CK inverted Microscope Manual
Replies: 7
Views: 596

Re: Looking for a Olympus Tokyo CK inverted Microscope Manual

Yes, I contacted Alan and he confirmed that he does not have a manual for the old Olympus CK/CKP/CKC Microscopes yet.
I will keep looking.

Thanks,
highbeta
by highbeta
Thu Sep 17, 2020 6:34 pm
Forum: Resources (online, books etc.)
Topic: Looking for a Olympus Tokyo CK inverted Microscope Manual
Replies: 7
Views: 596

Re: Looking for a Olympus Tokyo CK inverted Microscope Manual

Thanks Kirby,
I will keep my eyes open.If I am successful I will provide the manual to Alan Wood's collection.

highbeta
by highbeta
Thu Sep 17, 2020 6:15 am
Forum: Resources (online, books etc.)
Topic: Looking for a Olympus Tokyo CK inverted Microscope Manual
Replies: 7
Views: 596

Re: Looking for a Olympus Tokyo CK inverted Microscope Manual

Hi Kirby,

I know that great site very well, but unfortunately there is no no original CK manual.

I attached a cover of the manual that I am looking for.

Thanks,
highbeta
by highbeta
Wed Sep 16, 2020 7:56 pm
Forum: Resources (online, books etc.)
Topic: Looking for a Olympus Tokyo CK inverted Microscope Manual
Replies: 7
Views: 596

Looking for a Olympus Tokyo CK inverted Microscope Manual

Hello, I received an old Olympus CK inverted microscope with phase contrast. I am new to microscopes and I would like to understand more about the details. Does someone have the instruction manual for that type? I did some research already to find it but I was not lucky with that particular model. T...