MicrobeHunter.com Microscopy Forum

Manage to get this pic with a Reichert Neoplan 40X objective.
Non stacked, autofluorescence.

Does anyone happen to know or has a spec chart with the Epi cubes that were available for the Reichert 400 series

Heres a few pics , using my Diastar vertical illuminator and a HBo lamp.
Single shot ISO 800

40X Neoplan

I recently purchased a Microstar that came with a teaching adaptor and two heads, my question is... What is that box that sits below the head and attachment? And what is it for?

So paint or no paint? Here's a 40X Reichert Planacro, a bit more contrast than the 40X Neoplan

Welcome back Don, glad to hear you are doing well.

Here's all the upgrades available for it. I got a couple of quotes from the manufacturer in ( Shenzhen) and the prices are ok , but when you add shipping nit so much. I own the PH objectives and condenser as well as the Plan objectives 4,10,20,40,60&100X. The quality of the optics is inferior when c...

Aw thank you, have been experimenting with the instructions you gave me and has worked like a charm

Was able to get the following pics using
Frankenstar 420 & Laborlux K
40X Neoplan
40X Chilympus
40X Phaco 170mm
Stained with Acridine Orange 0.13%
Sony NEX3N

Can anyone identify this Desmid?

Just got my first batch of Acridine orange. Im still trying to figure out how to stain live ciliates. I think Im adding too much and so I get a lot of orange glow on the whole slide. If anyone has experience using this dye on protist any help will be welcomed. Best results so far. Leitz Laborlux K I...

UV addition to the Frankenstar
395nm
10W LED

Which excitation, dichroic mirror and emission filter combo do I need for this wavelength?
I have a spare cube that only has a mirror , so Im thinking of getting some cheap chroma filters and adding it to the cube

It is unlikely that the AO/Reichert lamphouse has anyyhing to do with Leitz, since the two companies were totally distinct until the merger, then no more Leitz. It is entirely possible that AO used imperial studs on that too. Many of the threads on AO stuff were imperial, although metric fittings c...

Any idea on what size or type of bolt is used on the rear mirror alignment mechanism found on Leitz, Reichert... Pretty much early 80s late 70s design.? They are practically the same mechanism so they can be transferred from one design to another. The recent acquisition came with the two bolts snapp...

In a first step differentiate between transmitted light and epi-fluorescence. Transmitted light: You need some excitation filter and an analyzer filter. The first under and the second over the specimen. Very much the same as polarization. " I've have very knowledge on this technique, do you know of...

Like so

walked right into that one didn't I!

Are you looking for info on how to assemble the hardware or on the dyes?

Spirogyra sp.

Great work! Do you think the base scope has transmitted DIC option as stock, or are you planning to do another Frankenstein mode :twisted: Indeed it does, from my understanding one of the best ...the condenser and turret are very difficult to come by, I'm currently formulating a plan to sell my wif...

Using polarizer, analyzer and lambda plate

https://youtube.com/shorts/Q0RR109LsCw?feature=share

Photo tube completed, as it sits the Frankenstar is only missing DIC. Im waiting for a couple of LED components to try at 395 and 460nm Epi. I recently purchased a 100W HBo bulb, but im getting no ignition, the power supply is putting out current, so Im guessing its the bulb. The photo tube has a 10...

Franken phototube works !!!! Im using a cat 191 eyepiece for projection.
https://youtube.com/shorts/sZaNwgevUCU?feature=share

Liquid crystal tunable filters... Has anyone played with one or has rigged one up for transmission or reflected light source? I see Thor labs has a few of them (astronomically expensive) so I've been thinking of using one if those ( stress/mood filters sheets ) as one , fully aware that the filter w...

Does anyone have any experience in custom making filter cubes, Im specially considering making Dapi filter for my Diastar ( I have a 1711 and another with just a dichroic mirror no emissions or excitation). I've seen companies like Chroma filters and Optolong offer emission filters, excitation filte...

This no name condenser works great with the Diastar, It allows me to rotate the Lambda plate and polarizer individually, Pol and Ana (red) Lambda (blue). The Threaded bottom if the condenser also allows for PH rings to be inserted.... Although a pain in the butt having to change it along with each o...

Which 30mm eyepiece did you use? Plan 10X 20WF from a no name Chinese seller, a 30.5 mm sleeve can be adapted to any 20mm as long as the overall diameter of the objective does not exceed 40mm at its widest point. Im still waiting for that final adapter so I can check for CA with various Eyepieces.....

I've been working on a photo tube for the Diastar/Microstar trino head. I've seen a couple of 3D printed options but since I don't have a printer, I decided to go with a metal option of photo/astronomy components that are readily available on Amazon. Still on the testing phase, but it can be used bo...

I was able to adapt the phase contrast kit onto the Diastar, that allows me to have BF, EPI, DF up to 20x and PH up to 40X The Rotating polarizer part you see on the stage normally screws on the bottom of the condenser, the Polarizer was taken out and oved onto a filter holder that sits on top of th...