Search found 90 matches

by Raul
Wed Apr 27, 2016 1:53 pm
Forum: Pictures and Videos
Topic: Tomato Stem
Replies: 14
Views: 1344

Re: Tomato Stem

Very good images Jim, did you use your home made microtome to cut the sample? (did you embed the stem in wax?)
by Raul
Mon Apr 18, 2016 5:15 pm
Forum: Pictures and Videos
Topic: Chondrocytes
Replies: 8
Views: 745

Re: Chondrocytes

billben74 wrote:Thanks very much for your protocol.
Thats perfectly clear to me.
I've only done plants for far, so its really interesting to hear about animal tissue. :)
You're welcome :).
by Raul
Mon Apr 18, 2016 5:11 pm
Forum: Beginner's corner
Topic: How to check if condenser is aligned?
Replies: 5
Views: 1299

Re: How to check if condenser is aligned?

It worked, I made a mark on the diffused lens, aligned the condenser with that, the iris closure was not on the center when looking through the eyepiece, but when the eyepiece was removed and I looked through the tube I saw that the iris closure was in the middle aligned. That iris closure looks fin...
by Raul
Mon Apr 18, 2016 11:48 am
Forum: Beginner's corner
Topic: How to check if condenser is aligned?
Replies: 5
Views: 1299

Re: How to check if condenser is aligned?

I have the same a LED with a diffused lens on top, but I can remove it an see the LED (light bulb).
What if I made a temporary mark on the diffused lens, in the middle and I align the condenser with that, would that work?
by Raul
Sun Apr 17, 2016 11:52 pm
Forum: Beginner's corner
Topic: How to check if condenser is aligned?
Replies: 5
Views: 1299

Re: How to check if condenser is aligned?

Yes it has, a made a drawing (Fig 1)some time ago of it, sorry that I do not have pictures for the moment. Also it seems that when fully closed the iris diaphragm is off center (Fig 2). The condenser is an Abbe 1.2 NA If I center the condenser using the light bulb (the illumination source) would tha...
by Raul
Sun Apr 17, 2016 10:20 pm
Forum: Beginner's corner
Topic: How to check if condenser is aligned?
Replies: 5
Views: 1299

How to check if condenser is aligned?

I was wondering these days if my condenser is not aligned, so I did some research on the internet and nothing. Nothing for light microscopy, so I ask you fellow members.
How to check/with what/ if the condenser is aligned?
Also I do not have a field diaphragm.

Regards,
Raul
by Raul
Sat Apr 16, 2016 11:08 pm
Forum: Pictures and Videos
Topic: Variations of Safranin & Fast-green staining protocol
Replies: 14
Views: 1203

Re: Variations of Safranin & Fast-green staining protocol

Great images, please John post the higher magnification photos, also you've done the second image just by varying the time of exposure to a stain or time of differentiation (and the second image is done with just safranin and fast green ?)

P.S. where does the blue tint come from?

Regards,
Raul
by Raul
Sat Apr 16, 2016 8:25 pm
Forum: Beginner's corner
Topic: How does a COL filter look like?
Replies: 6
Views: 1101

Re: How does a COL filter look like?

Thank you zzffnn for the link.
charlie I do not intend to make a phase telescope yet, but thank you for the tip.
by Raul
Fri Apr 15, 2016 12:04 pm
Forum: Beginner's corner
Topic: How does a COL filter look like?
Replies: 6
Views: 1101

Re: How does a COL filter look like?

Thank you zzffnn, that information is most helpful.
by Raul
Thu Apr 14, 2016 11:31 pm
Forum: Beginner's corner
Topic: How does a COL filter look like?
Replies: 6
Views: 1101

How does a COL filter look like?

Exactly as the title says, can someone photograph his filter or find/make a diagram on how a COL filter should look like please?



Regards,
Raul
by Raul
Thu Apr 14, 2016 11:12 pm
Forum: Pictures and Videos
Topic: Chondrocytes
Replies: 8
Views: 745

Re: Chondrocytes

Yes it is a long process Lymia, but it seems to do the job :) .
by Raul
Wed Apr 13, 2016 10:17 pm
Forum: Pictures and Videos
Topic: Chondrocytes
Replies: 8
Views: 745

Re: Chondrocytes

BillT I always try to use things that are handy, that's what an amateur is like, reinventing a purpose for some items, but it has some limitations and could lead to bad results where they could have been good. Thank you billben74, well the whole procedure is the following: 1. Collected a chicken bon...
by Raul
Wed Apr 13, 2016 3:11 pm
Forum: Pictures and Videos
Topic: Chondrocytes
Replies: 8
Views: 745

Re: Chondrocytes

Thank you BillT. I'm glad you appreciate them.
by Raul
Wed Apr 13, 2016 1:54 pm
Forum: Pictures and Videos
Topic: Chondrocytes
Replies: 8
Views: 745

Chondrocytes

Cartilage showing chondrocytes. TBO stained. Mounted in Nail Polish.
Hope you enjoy.
800x magnifiction
Image

800x and 4x camera zoom

Image
by Raul
Tue Apr 12, 2016 9:45 pm
Forum: Specimens, samples and slides
Topic: NPM for animal tissue
Replies: 11
Views: 4635

Re: NPM for animal tissue

MicroMan2 I solved it, I mounted cartilage tissue in Nail Polish and it did not crack, nor it formed bubbles. Here's my protocol: After the staining, dehydrate in 70% IPA 1min, 90% IPA 1 min, 100% IPA 1 min then add the Nail Polish (I used one that had IPA as a solvent in it so it mixed perfectly) H...
by Raul
Sun Apr 10, 2016 4:48 pm
Forum: Specimens, samples and slides
Topic: NPM for animal tissue
Replies: 11
Views: 4635

Re: NPM for animal tissue

I've been unsuccessful so far with Nail Polish, I tried to mount skeletal muscle stained with H&E but it cracked horribly after couple of days, or it made bubbles around, above and in the tissue when I tried to mount kidney samples. Best and most convenient is with Glycerol Jelly.
by Raul
Thu Apr 07, 2016 9:29 pm
Forum: Pictures and Videos
Topic: Leaf TS stain-trial, Safranin vs Fast-green - the battle for supremacy!
Replies: 12
Views: 844

Re: Leaf TS stain-trial, Safranin vs Fast-green - the battle for supremacy!

Great photos wow, I cannot remember seeing such good photos in textbooks (which are supposed to be the best of the best) John you have evolved very much, there is a lot of hard work and trial and error but you have done it. Congratulations for all that you've done and thanks for sharing your work on...
by Raul
Tue Mar 15, 2016 11:55 pm
Forum: Pictures and Videos
Topic: The microscopic study of bone (self made slide)
Replies: 5
Views: 453

Re: The microscopic study of bone (self made slide)

Thank you billbillt and billben74 i appreciate your feedback very much. billben74 yes a tooth is a very good and interesting idea, that would be a future project as soon as I find a donor for that (hope it won't be me because I have my permanent teeth).
by Raul
Tue Mar 15, 2016 10:04 pm
Forum: Pictures and Videos
Topic: The microscopic study of bone (self made slide)
Replies: 5
Views: 453

Re: The microscopic study of bone (self made slide)

Thank you very much billbillt. As promised the protocol is the following: The samples (left is a chicken femur and right bone unknown origin from a pig) http://i.imgur.com/NhEQ0Sa.jpg First I tried the femur but was only able to section it in half (still trying on it) http://i.imgur.com/JE3Bus6.jpg ...
by Raul
Tue Mar 15, 2016 8:49 pm
Forum: Pictures and Videos
Topic: The microscopic study of bone (self made slide)
Replies: 5
Views: 453

The microscopic study of bone (self made slide)

Hello all, I wanted to share some images from a new project of mine. The good donor for this sample is an unknown pig. I will also post pictures of the procedure for the processing of the bone if anyone is interested. Hope you enjoy it. http://i.imgur.com/F4GQa0J.jpg http://i.imgur.com/2Sf7x1R.jpg h...
by Raul
Sun Feb 28, 2016 8:50 pm
Forum: Pictures and Videos
Topic: Ovule and Ovaries, glorious colour, mysterious cells
Replies: 19
Views: 779

Re: Ovule and Ovaries, glorious colour, mysterious cells

Thank you John B for answering, yes that was exactly what I wanted to know, beautiful results, very good staining protocol. Also why did you filter the Safranin solution? I also bought it from BM and it did not have any problems with it of impurities( I bough the powder as in your image). Congratula...
by Raul
Sat Feb 27, 2016 5:31 pm
Forum: Pictures and Videos
Topic: Ovule and Ovaries, glorious colour, mysterious cells
Replies: 19
Views: 779

Re: Ovule and Ovaries, glorious colour, mysterious cells

Also it looks to me that the cell which does not seem to have cytoplasm and the red nucleus (stained with safranin) is undergoing apoptosis (programmed cell death) http://labs.bio.unc.edu/dangl/pub/pdf/CellDeathDiff_vanDoorn_Nomenclature_2011.pdf Here's a paper that I found on this. (the image is ta...
by Raul
Sat Feb 27, 2016 5:20 pm
Forum: Pictures and Videos
Topic: Ovule and Ovaries, glorious colour, mysterious cells
Replies: 19
Views: 779

Re: Ovule and Ovaries, glorious colour, mysterious cells

John B that is very beautiful, I especially like the complementarity of haematoxylin and fast-green. Superb pictures,great clarity, please do post the protocol and all the information from the slide.
by Raul
Fri Jan 29, 2016 12:27 pm
Forum: Digital processing
Topic: Desmid image: DIC background correction
Replies: 13
Views: 5372

Re: Desmid image: DIC background correction

Very nice images gekko, tell me did you use this technique for DIC (http://www.microscopy-uk.org.uk/mag/ind ... iydic.html) a filter, or do you have accesss to the real thing?
by Raul
Fri Jan 29, 2016 12:24 pm
Forum: Beginner's corner
Topic: Safranin working solution?
Replies: 6
Views: 1543

Re: Safranin working solution?

Thank you mrsonchus, that is some really good information (especially from the fact that is experimented by you). I intend to do some Gram stain for bacteria, but mainly I will use Safranin histologically, being very similar with what you described. Thank you very much to all the people that respond...
by Raul
Wed Jan 27, 2016 10:35 pm
Forum: Identification help
Topic: Unidentified
Replies: 18
Views: 2938

Re: Unidentified

Well sadly I have no photos of that, but maybe my description will do. RBC were normally shaped (biconcave), although some were broken/shattered ( probably due to defrosting),and WBC none so far, but I only took 2 samples of the sediment and didn't see a sign of WBC, therefore I do not know if they ...
by Raul
Wed Jan 27, 2016 10:02 pm
Forum: Identification help
Topic: Unidentified
Replies: 18
Views: 2938

Re: Unidentified

Thank you gekko and that is not a dumb question ( I think it is a rather good one). The sample of blood was taken from a steak (from the cutting process some bits of muscle got ripped and stayed in suspension in the liquid) I was also surprised to see something sediment since the blood was clear at ...
by Raul
Wed Jan 27, 2016 3:38 pm
Forum: Identification help
Topic: Unidentified
Replies: 18
Views: 2938

Re: Unidentified

Thank you, Crater Eddie. Well gekko, it is not really my best work on muscle (http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=6&t=1732&p=13463&hilit=muscle#p13463) here's another post of mine talking about this subject, but I will describe my procedure as detailed as possible. Took blo...
by Raul
Wed Jan 27, 2016 2:20 pm
Forum: Identification help
Topic: Unidentified
Replies: 18
Views: 2938

Re: Unidentified

Crater Eddie nothing in particular, I just looked at the sediment that formed from centrifugation (epithelial cells, skeletal muscle cells and some erythrocytes but that seemed to be all, except this pollen sample)

Here's another picture of some skeletal muscle tissue
by Raul
Wed Jan 27, 2016 12:54 pm
Forum: Identification help
Topic: Unidentified
Replies: 18
Views: 2938

Re: Unidentified

I found this, apparently it could be from thyme.