Search found 2799 matches

by apochronaut
Tue Jan 21, 2020 1:20 pm
Forum: Microscopes and optics
Topic: Help me identify Reichert's objetive and microscope
Replies: 11
Views: 203

Re: Help me identify Reichert's objetive and microscope

Hello again. One observation is that the two pictures you have posted seem to be of two different microscopes - the first has the second knob on the base, the other (showing the underside of the scope with additional filter wheel) has the edge of dial protruding from the same area. The latter pictu...
by apochronaut
Mon Jan 20, 2020 5:25 pm
Forum: Microscopes and optics
Topic: Help me identify Reichert's objetive and microscope
Replies: 11
Views: 203

Re: Help me identify Reichert's objetive and microscope

. The picture from the underside your microscope reveals a double filter carousel, characteristic of the Fluorvar, although the Fluorvar, which in all other ways is the same as your microscope, has the later coaxial rotary focus, used after about 1975. It is possible that your microscope is in fact ...
by apochronaut
Mon Jan 20, 2020 4:26 pm
Forum: Illumination Techniques
Topic: why are my photo's and video's always yellowish?
Replies: 7
Views: 183

Re: why are my photo's and video's always yellowish?

Re yellow photos - you can do one of four things to fix the background colour: 1) use a blue filter - they are sometimes called daylight filters, and use the max brightness of your bulbs 2) You can set you camera white balance to tungsten about 3200 K 3) You can fix the colour quickly if you use Ph...
by apochronaut
Mon Jan 20, 2020 4:09 pm
Forum: Specimens, samples and slides
Topic: 100 x oil for water life
Replies: 5
Views: 102

Re: 100 x oil for water life

You can use an oil immersion objective without securing it in theory, as long as your sample preparation and tools are accurate. Having to use paraffin or risers under the slip, certainly detracts from the ease and joy of using an oil immersion objective and helps to magnify the reasons so many unse...
by apochronaut
Fri Jan 17, 2020 2:34 pm
Forum: Camera systems and imaging
Topic: DSLR or Integrated?
Replies: 11
Views: 269

Re: DSLR or Integrated?

Of course one has to choose a mirrorless camera to suit one's needs just as is the case with choosing any tool. Canon isn't the only company making cameras.
by apochronaut
Fri Jan 17, 2020 1:04 am
Forum: Beginner's corner
Topic: Advice on next upgrade to a T390, looking to observe yeast and bacteria.
Replies: 10
Views: 217

Re: Advice on next upgrade to a T390, looking to observe yeast and bacteria.

A picture IS worth a thousand words............sometimes. Sometimes only 2 , or 1 multisyllable word will do.

A problem arises when a picture requires one or many verbal keys, especially when it is being presented to someone quite unfamiliar with what they are looking at.
by apochronaut
Fri Jan 17, 2020 12:08 am
Forum: Camera systems and imaging
Topic: DSLR or Integrated?
Replies: 11
Views: 269

Re: DSLR or Integrated?

I would second the vote for a mirrorless body. Often, a person comes to microscopy from being somewhat or intimately acquainted with photography. They see the microscope as just another lens option for their camera. They have it backwards. The microscope potential needs to come first and then choose...
by apochronaut
Wed Jan 15, 2020 4:38 pm
Forum: Beginner's corner
Topic: Advice on next upgrade to a T390, looking to observe yeast and bacteria.
Replies: 10
Views: 217

Re: Advice on next upgrade to a T390, looking to observe yeast and bacteria.

The following images demonstrate 3 different phase types used to view the same sample. This is a yeast and bacteria culture, grown from a center plug removed from a baked loaf of sourdough bread.
by apochronaut
Wed Jan 15, 2020 4:04 pm
Forum: Beginner's corner
Topic: Advice on next upgrade to a T390, looking to observe yeast and bacteria.
Replies: 10
Views: 217

Re: Advice on next upgrade to a T390, looking to observe yeast and bacteria.

Apochronaut, thank you for taking the time to write such a thorough reply. As suggested, I will probably source eypieces with a higher eye relief and will stick to my current setup without changes until I learn enough to start thinking on the next step. I will start by researching the terminology y...
by apochronaut
Tue Jan 14, 2020 4:13 pm
Forum: Beginner's corner
Topic: Advice on next upgrade to a T390, looking to observe yeast and bacteria.
Replies: 10
Views: 217

Re: Advice on next upgrade to a T390, looking to observe yeast and bacteria.

Getting your microscope where you want it, will be a big d.i.y. project. and probably not worth the effort. Your limitations are based on the N.A. and degree of colour correction of the objectives, the precision and design of your sample preparations and the objective contrast plus the contrast tech...
by apochronaut
Tue Jan 14, 2020 2:50 pm
Forum: Beginner's corner
Topic: Advice on upgrading a stereo microscope
Replies: 15
Views: 285

Re: Advice on upgrading a stereo microscope

Because the OP's microscope has a very similar looking twin, rated for 50X, a twin that accepts both 10X and 25X eyepieces, I suspect the two share the same objectives, and that his microscope's objectives have the resolution. Optical engineering, however, is a matter of approximation and optimizat...
by apochronaut
Mon Jan 13, 2020 9:39 pm
Forum: Beginner's corner
Topic: Advice on upgrading a stereo microscope
Replies: 15
Views: 285

Re: Advice on upgrading a stereo microscope

The problem is somewhat analogous to attempting to see what is written on a letter that you can't read in a movie, by walking closer to the screen. If the resolution is not there in the first place, no amount of magnification will reveal it. Generally, manufacturers have been very accurate in matchi...
by apochronaut
Mon Jan 13, 2020 7:14 pm
Forum: My microscope
Topic: Reichert Polyvar Microscope
Replies: 22
Views: 362

Re: Reichert Polyvar Microscope

I am pretty sure you have a cement failure in the beam splitting prism: probably what you are calling a semi-reflective layer. In many heads, there is just this one cemented surface, causing the same visual anomaly on both sides but with one appearing quite apparent and the other appearing as a kind...
by apochronaut
Mon Jan 13, 2020 6:56 pm
Forum: Beginner's corner
Topic: Please help me identify this. Yeast or fungus?
Replies: 4
Views: 75

Re: Please help me identify this. Yeast or fungus?

Wes has it . Additionally, the grape-like cluster at 12 o'clock are yeasts. There are some bacteria present, though. Towards about 3:15 and then again towards 4:00 are two spore bearing bacilli, undergoing division.
by apochronaut
Mon Jan 13, 2020 12:06 pm
Forum: My microscope
Topic: Reichert Polyvar Microscope
Replies: 22
Views: 362

Re: Reichert Polyvar Microscope

Since various cements have been used in compound optics, de-lamination has different characteristics, depending on the type of cement used. In some cases, the factory providing the cement, made a dud batch, which did not stand the test of time or the formula turned out to have a photo reaction over ...
by apochronaut
Mon Jan 06, 2020 5:49 pm
Forum: Beginner's corner
Topic: Cleaning optical lenses
Replies: 7
Views: 186

Re: Cleaning optical lenses

Although the Lumipan originated in the 30's, the design persisted long enough into the 50's that some of the optics produced for it, must have been coated. Although the theory of using contact polymers to clean lenses is sound, the practice of it requires a high degree of precision and knowledge of ...
by apochronaut
Fri Jan 03, 2020 9:13 pm
Forum: Microscopes and optics
Topic: Zeiss ocular projective
Replies: 6
Views: 178

Re: Zeiss ocular projective

Older eyepieces, in a similar fashion to telescope eyepieces were marked by the focal length, rather than the magnification. 1/2", 1" etc. or 12mm, 25mm etc. A 100mm focal length is about 4", so should come out to 2 to 3X, or so, depending on the actual design. It is probably a huygens or modified h...
by apochronaut
Thu Jan 02, 2020 4:05 pm
Forum: Beginner's corner
Topic: Does a DSLR adapter need a lens in it?
Replies: 38
Views: 1726

Re: Does a DSLR adapter need a lens in it?

Focus stacking does not control lateral c.a. or coma, both serious issues with many low grade non plan objectives. The original poster was not asking about focus stacking, though. Perhaps you can assist the o.p'er with some information tabout how he can obtain corner to corner planarity and colour c...
by apochronaut
Thu Jan 02, 2020 2:01 pm
Forum: Beginner's corner
Topic: Does a DSLR adapter need a lens in it?
Replies: 38
Views: 1726

Re: Does a DSLR adapter need a lens in it?

With which objectives? Very few microscope objectives provide an image that does not need correcting. There are some well known notable ones and even some of those require field flattening. The camera sensor is unlike an eye/brain complex, in that it must receive a completely flat image corner to co...
by apochronaut
Tue Dec 31, 2019 11:05 pm
Forum: Camera systems and imaging
Topic: Connecting DSLR directly through eyepiece tube, too heavy?
Replies: 14
Views: 405

Re: Connecting DSLR directly through eyepiece tube, too heavy?

It should all work out. If the 1/2 kg. of your camera proves to be a problem, there are other, lighter inexpensive options, which can still use the same adapter, sometimes with a small modification.
by apochronaut
Tue Dec 31, 2019 1:47 am
Forum: Camera systems and imaging
Topic: Connecting DSLR directly through eyepiece tube, too heavy?
Replies: 14
Views: 405

Re: Connecting DSLR directly through eyepiece tube, too heavy?

The Nikon d3100 doesn't have a shutter with elecronic first shutter curtain function. So the shutter will be activated when released from live view. This can blur the photos considerably, especially at higher magnifications. I think this will be as much a problem as the leverage on the tube and the...
by apochronaut
Mon Dec 30, 2019 1:29 pm
Forum: Camera systems and imaging
Topic: Connecting DSLR directly through eyepiece tube, too heavy?
Replies: 14
Views: 405

Re: Connecting DSLR directly through eyepiece tube, too heavy?

Without knowing how the unit you have chosen mounts or how far towards the end of the tube it connects, it is hard to say. 500 gm. cantilevered on the end of the eyepiece tube , exerts quite a bit more force than 500 gm. connected to the tube an inch farther down. I still doubt if there could be any...
by apochronaut
Fri Dec 27, 2019 1:54 am
Forum: Pictures and Videos
Topic: Delaminated eyepiece lens
Replies: 6
Views: 257

Re: Delaminated eyepiece lens

that's the most wonderful delaminated lens, I have ever seen.
by apochronaut
Fri Dec 27, 2019 1:51 am
Forum: Microscopes and optics
Topic: American Optical Series 20 illumination
Replies: 5
Views: 152

Re: American Optical Series 20 illumination

I guess you now see what I meant by the dark grey collar over the illuminator/body seam separating one version from another, now, Pete? As much as I like the 20, and I have had 5 or 6 go through my hands, it isn't as versatile an instrument as the 120. They can use the same optics but if one wants t...
by apochronaut
Thu Dec 26, 2019 3:33 pm
Forum: Camera systems and imaging
Topic: Connecting DSLR directly through eyepiece tube, too heavy?
Replies: 14
Views: 405

Re: Connecting DSLR directly through eyepiece tube, too heavy?

It seems then, that it should be o.k. I think that your DSLR, should be not much over 1 lb. The Apex attachment does stretch over a bit of the optical tube and couples the camera directly to a bayonet adapter so it is not afocal . The system is just substituting the ocular tube for a trinocular tube...
by apochronaut
Thu Dec 26, 2019 2:33 pm
Forum: Camera systems and imaging
Topic: Connecting DSLR directly through eyepiece tube, too heavy?
Replies: 14
Views: 405

Re: Connecting DSLR directly through eyepiece tube, too heavy?

That's a monocular with a fairly long viewing tube?
by apochronaut
Thu Dec 26, 2019 2:17 pm
Forum: Camera systems and imaging
Topic: Connecting DSLR directly through eyepiece tube, too heavy?
Replies: 14
Views: 405

Re: Connecting DSLR directly through eyepiece tube, too heavy?

The best way to do that is to mount your camera on a tripod and suspend it over the eyepiece tube, for afocal photography. Attaching the camera to the eyepiece tube is possible but complications such as the weight of the camera and mirror vibration will contribute to making a physical attachment mor...
by apochronaut
Thu Dec 26, 2019 12:57 pm
Forum: My microscope
Topic: What Microscope do I use.
Replies: 8
Views: 314

Re: What Microscope do I use.

That is true , and since the N.A. of the oiled objective is fixed, when oil is not used with the condenser , the quality of the condenser becomes quite important. That is the rational for the more innovative microscope companies from the 80's on , offering high N.A. dry achromat condensers as an opt...
by apochronaut
Wed Dec 25, 2019 8:12 pm
Forum: For forum members who want to buy and sell equipment
Topic: Good place to buy a microscope in Austria
Replies: 9
Views: 354

Re: Good place to buy a microscope in Austria

You are right in the heart of Reichert country, which gives you a leg up on the possibility of landing one of the more compact, infinity corrected Reichert models made between 1972 and into the 90's. I say more compact, because a Univar or Polyvar both have pretty large footprints but there were mor...
by apochronaut
Wed Dec 25, 2019 5:35 pm
Forum: My microscope
Topic: What Microscope do I use.
Replies: 8
Views: 314

Re: What Microscope do I use.

Welcome to the forum. Brisoline are quite good . The older ones were made by Kyowa. Using a dry 60X of large aperture( .90 - .95 N.A.) and 15 or 16X eyepieces can provide close results to those achieved by a 100X 1.25 oil immersion objective with aqueous subjects, especially if the sample is a littl...