MicrobeHunter.com Microscopy Forum

What you're doing there is actually almost the same as Dodt contrast. You aperture is in a fourier plane and you use a diffuser to get a smooth gradient. Now switch to a quarter circle plate and you get the additional advantage of having a gradient in two orthogonal directions, not just one!

Yes, I'm a german scientist. I think they filed a US patent as well and google also has a translation. The condensed version of it is: The tube is fully self contained and inserted into a part of the illumination path were the light source is focused to infinity, basically directly after the collima...

After endless search in his papers and not finding anything of value, I checked for patents. E voila, there it is, with all detail.
DE000019514358C2

At https://depatisnet.dpma.de/ the fulltext and figures are available.

I tried it. Turns out my microscope has a small slit that lets me position a thin object between lamp collimator and diffusor. I 3D-printed a quarter annulus (slightly to large diameter) and put it in. It does contrast phase objects like epithelial cells or embedded red blood cells, which are hard t...

It's not so different from oblique illumination. For many in vitro two-photon systems it's pretty standard. Some patching people buy DIC because it costs more, but with DGC there are no prisms in the way of the laser and it's said to be better for thicker slices, which is typically the case for acut...

Yeah, I'm not having high hopes for the IS cameras. But I was thinking that it might support UVC. What does it support actually? Direct Show? How does it detect Touptek cams?

I would like to use - ideally - USB camera objects (UVC), like a regular webcam outputs. Alternatively, I have some imagingsource cams.

I tried to use Toupview with non Touptek Cameras and I can't load a device. I used version 4.something. Am I doing something wrong? What type of camera does it support or is it restricted to their products alone (which would be strange since so many companies resell it with different names...) Thank...