MicrobeHunter.com Microscopy Forum

Pretty sure I have seen examples where stacking was done by refocusing the camera lens in an afocal setup, which would be similar overall effect, maybe someone else knows/remembers more about that. Any examples of this sort of approach would be great, direct projection or afocal. When it comes to a...

... But for the case-in-point [a stereo microscope] I think the 'tolerance' is likely to be quite wide. Ah, yes, I missed that this was a stereo microscope. So forget the thing about calculating assuming 160 mm tube length, that maybe not be correct. If the basic scaling of tolerance to change in t...

Good response, Hans ... But for the case-in-point [a stereo microscope] I think the 'tolerance' is likely to be quite wide. Methinks newtonsapple my be onto a good idea. MichaelG. Hopefully someone more knowledgeable than me can come along and explain fully why this is or is not a good idea. I need...

In 3 although the whole object is not moved by twiddling the microscope, the bit of interest on the object is moving relative to the lens, and does move to different heights above the lens. I think ;) F2.jpg Yeah, I am trying to wrap my head around what will actually be going on. With a stereo micr...

Sorry, AFAIK auto focus works by moving the camera lens relative to the imaged object. This is true for the adapter shown in your posted link as well. The camera lens is the analogue of the microscope objective in this respect. So, again, leaving the objective static and changing just the height of...

So I am at that point in learning about microscope imaging where I am able to ask what might be a stupid question. So in direct projection, my understanding is that there is a physical position where the sensor is parfocal with the eyepieces. Does shifting the sensor position up and down from that p...

I am wondering if moving the sensor position in a direct projection arrangement would provide the required focus shifts needed for focus stacking? For context, I have an Nikon SMZ800 and a camera port I am looking to set up for direct projection onto an APS-C mirrorless sensor. If I include a short ...

Scarodactyl, when you get a chance, could you also post some photos of the objective changer and how it mounts? I might be able create one. Thanks!

Thanks for this review. I have an SMZ800 and a Scienscope branded clone. Unfortunately they both have misaligned heads and after having them sit on the bench for months without becoming brave enough or having time to tackle alignment I called around and found a local shop to align them for an accept...

So I grabbed a camera beam splitter under the Bolioptics name. Bolioptics has a .7x objective I haven't seen elsewhere: https://bolioptics.com/0-7x-infinity-corrected-achromatic-microscope-objective-lens-working-distance-135mm/ The .5x plan APO should be interesting. Given the intent of my work, I w...

The scienscope parts are compatible, yes. The c er is a ring that sita in the 76mm round mount on your stand so the microscope sits higher. Your working distance doubles with a 0.5x objective so the scope has to sit higher. Thanks, that makes sense as a lot of stands won't go high enough. Have you ...

I have an SMZ800 with the P-BERG ergo eyepieces that I actually need to repair as the left and right images don't align and converge. I have the repair manual, but not the specific collimation tools, so it is a little daunting of a task. Any input would be great, but not the main focus of my post. I...