MicrobeHunter.com Microscopy Forum

Welcome to the forum, Mickey! You can use camera lens step-down rings to connect a Sigma 30mm f/2.8 (micro 4/3) lens, then to a visual 10x eyepiece (and connect E-M5 II to the Sigma 30mm lens). They can be easily disconnected by unscrewing adapter threads. You can use, for example, 46-28, then 28-27...

It might be "III" for 3. From the design this microscope looks like as if it were inspired by pre WW 2 Zeiss microscopes. It was for sure a model at the expensive end of the range. Bob Yes, it looks that way to me as well. Higher end Zeiss copy/clone. Maybe the condenser and head dovetail mounts wo...

40mm lens on 1.6x crop factor Canon APSC sensor is about right, which is almost the same as 30mm lens for 2x crop micro four thirds sensor. Some external zoom lens may not stay at 40mm, under the weight of an afocal camera, unless you tape the lens barrel. Give it a try and see it that works. 20-25m...

Welcome, Diego!

You may want to join the other forum as well. Carlos, who also lives in Uruguay, has been taken tons of microscopy videos:

http://www.photomacrography.net/forum/p ... ile&u=2314

Very nice, 75RR! Did you try using flash to get a photo of it?

Lauren, Apparent visual quality is determined by resolution (NA) devided by magnification. So 10x NA 0.25 objectives usually look brighter and more contrasty than 40x NA 0.65 objectives. Absolute resolution is determined by aperture (NA). But contrast is no less important in microscopy; sometimes we...

Welcome from near Houston, Lauren! Friendswood 77546, to be precise. I and Kurt have been trying to meet in person, but have not gotten a chance (I blame Kurt for pretending to be always busy). Now we are both almost ready, but I am relocating to Boston in a few days :( We talked about set up a fiel...

Aluminum sheets are great! I have used them as spacers. Need to keep it flat though.

(Jewelry) Bead threads may work too.

In USA, stores like Hobby Lobby offer many bead threads of different thickness.

Thank you very much, gents.

John B, your banding is quite subtle. My LED reveals very obvious band at most shutter speeds.

Sorry for the confusion about dimmer.

Yes, the friend of mine who made my LED said bands came from the PWM dimmer and different pot position may produce different amount of banding.

I think most APSC sensor would be fine with iso800. Even my small micro four thirds sensor (Olympus E-M1 mk1) does okay with iso800, though not much higher.

John B or anyone who has retroDiode LEDs,

If you use your camera in eletronic shutter mode with the LED, do you see those frequency bands?

Thank you.

Rod, Sorry, I may not be clear. I meant you can remove the specimen stage from that epi scope, replace it with a goniometer and use the rig as a vertical rig. I cited Chris S' rig only to show how he uses goniometer as compact specimen stage. I forgot about fluorescence. Pau from the other forum mad...

Wes, I don't think optically clear "lamda plate" material that you can place above obejctives can be found cheaply. Unless you found a surplus eBay seller who knows nothing about his stuffs. I would jump on it before you, if there is one :twisted: I used cheap cellophane "lamda plate" the cheap way,...

I agree with MicroBob. Objectives with around 4x power can be roughly divided into two categories (though optical compensation and reality is more complex than that): 1) those that are almost self-corrected and need minimal compensation from eyepieces; and 2) those that have abberations added, so th...

Welcome back, Rod! Your reappearance even lured Kurt out from his cave :mrgreen: Over at photomacrography, people would usually remove that epi scope's specimen stage and put a small multidirectional goniometer stage to precisely position opaque subjects (typically insects). Here is an example: http...

@Hobbyst46, You may want to buy the exact 4x Amscope objective from the exact seller to make sure it is the good one: https://www.closeuphotography.com/seventeen-dollar-plan-4x-objective/ Also note that Robert OToole used it without eyepiece (direct projection onto sensor). LOMO 3.7x likely won't wo...

Phil, He most likely does not need professional pol scope or strain free optics. His use is artistic and not scientific. My suggestion for him is get in with an AO and probably upgrade to Mitutoyos or high end infinity Nikon/Oly later on, if he can sell his prints really well. Mitutoyos are not easy...

To everyone in this thread, I know a newbie microscopist in Houston Texas looking for a scope for polarization (chemical crystals, not phase contrast is needed, though 4, 10, 20, 40 objectives should be plan achromats, an 100x is an option but not required). I suggested him to consider an AO series ...

Hey, I know you, Saul Welcome!

Welcome from Houston! AO objective list can be found here: https://user.xmission.com/~psneeley/Personal/Objectives.htm Scope model list: https://user.xmission.com/~psneeley/Personal/Microscope.htm Try to use the correct matching eyepiece for objectives and scopes: https://user.xmission.com/~psneeley...

The -phot microscopes are all finite, and the heads and head accessories across the alphaphot, orthophot and labophot are all compatible. This is what I remember too, though I am only 99% sure about it. Heads of Labophot, Optiphot and Nikon S are compatible for sure, as I have had all of those (but...

Leica still calls S9 a Greenough scope. The video shows that the high resolution right size has much bigger (diameter) optics than the DoF left side and that right and left sides go through different optical path. It still sounds like stopping down the DoF (left) side to me, in reality. It might be ...

The wording that I used in my previous post should be obvious enough to reveal that I was only summarizing relevant information that can be found online by most people. The OP should determine by himself or herself if such information is accurate or not. Even a licensed phycian would not provide "me...

Sorry to be blunt, but no professional would be willing to ID based on photos. If you know how to do proper culture and gram staining, then you should know such ID is not possible from images. I am also not sure if your culture was contaminated by other bacteria (such a culture should be done in nea...

In my humble opinion, darkfield offers lower resolution (inside a structure like a diatom) and less DoF, but make it up with much better edge/border contrast than bright field, so you may perceive it as having higher resolution and deeper DoF. Some people like it and some don't. For hobbyists, it is...

Your 1), 2) and 3) are all possible. But 1) and 2) may allow user adjustment; have you tried those (e.g., move light bulb back and forth and move Amscope adapter / camera up and down?). Do you see uneven light using your NA 1.4 condenser with a 10x objective? Also your adapter tube or any tube in be...

Hobbyst46, They are look good to me. f/5.6 seems slightly sharper than f/1.8 without 100% crop, but difference is not significant. I think since front lens (100x NA 1.3) of your system is already deep into diffraction, you won't lose anything when stopping down from f/1.8 to f/5.6, you may actually ...

Hobbyst46, Your M10 camera's sensor is APSC and larger than my micro 43 sensor (supposedly your camera's iso performance will be better). I suggest using iso 200, aperture priority mode (let camera meter exposure based on dot or center-weighted metering mode and determine which shutter speed to use,...

Bob, Some outdoor LED headlights can be smaller (with head straps removed) and can be focused with two levels of adjustable power. There may be other battery-powered LED options, such as flat flashlights or small flat work lights, but I have not found a perfect one yet. Those headlights are usable t...