Search found 66 matches

by kinase
Mon Jun 12, 2017 10:12 pm
Forum: Pictures and Videos
Topic: Some Algae DNA
Replies: 4
Views: 810

Re: Some Algae DNA

Yeah you don't buy 50mL of any kind of fluorescent stain. That amazon link has $40 shipping, and that's still oddly cheap. Thats probably for making a lot of DNA gels or some other application, maybe lots of qPCR. I'm not sure that you can just buy the stuff. It's typically 10000x concentration, i.e...
by kinase
Mon Jun 12, 2017 2:03 pm
Forum: Pictures and Videos
Topic: Some Algae DNA
Replies: 4
Views: 810

Some Algae DNA

I neglected to take white light photos, probably because this dye bleaches fast so I was focused on that. This was stained using SYBR green, we're at 40x. These cells occur in diploids. You can also see what I believe to be a nucleolus with some odd ringed structure around it. Not sure what that mig...
by kinase
Mon Apr 24, 2017 7:38 pm
Forum: Pictures and Videos
Topic: Slide-surfing surprise - fungus! - maybe.....
Replies: 11
Views: 1205

Re: Slide-surfing surprise - fungus! - maybe.....

I'm not too familiar with it. But at the very least, fixation and staining are chemistry based and the rates at which those things occur are subject to temperature. Typically shortened stain and fixation times reduce cellular deformations and increase penetration into paraffin sections. Usually I se...
by kinase
Mon Apr 24, 2017 3:13 am
Forum: Beginner's corner
Topic: Culture medium for algae and other critters
Replies: 17
Views: 3400

Re: Culture medium for algae and other critters

Oh I wish I saw this sooner... My lab is an algae zoo, complete with various kinds of media (many of which are highly similar). Is this fresh or salt water we're talking? There's lots of recipes out there, but you kind of need a small chemistry lab to make them. You can probably boil solid fertilize...
by kinase
Mon Apr 24, 2017 3:08 am
Forum: Pictures and Videos
Topic: Slide-surfing surprise - fungus! - maybe.....
Replies: 11
Views: 1205

Re: Slide-surfing surprise - fungus! - maybe.....

If you look at root parts you should be able to find fungus pretty easily, pretty much all plants have symbiotic fungi. Leaves, probably not so much. Have you ever considered microwaving your samples while staining or the stain itself? I know people do that for EM and sometimes with giemsa for regul...
by kinase
Sat Feb 18, 2017 10:52 pm
Forum: Pictures and Videos
Topic: looking for a better camera
Replies: 4
Views: 996

Re: looking for a better camera

I think using SLR's is a good choice. Mirrorless cameras would be better probably, I know someone who uses a Sony Alpha as a microscope camera it's it's really nice. For bright light, I think regular photographic cameras are better than dedicated microscope cameras.
by kinase
Fri Feb 17, 2017 1:30 am
Forum: Pictures and Videos
Topic: Algae with very long flagella
Replies: 6
Views: 915

Re: Algae with very long flagella

Beautiful images,kinase. Is the last image a 'stack' of images...or did all the tendrils just happen to lie in near same focal plane? Where was this algae sampled from (the adjacent areas seem so clear of debris)? For your images here..what brand/ what spec coverslips do you use? thanks for these p...
by kinase
Thu Feb 16, 2017 4:06 am
Forum: Pictures and Videos
Topic: Algae with very long flagella
Replies: 6
Views: 915

Algae with very long flagella

Apparently they are, in fact, pseudoflagella. https://c1.staticflickr.com/3/2162/32927234335_9b37cfcd62_c.jpg https://c2.staticflickr.com/4/3736/32773688592_afeb3cb63c_c.jpg https://c1.staticflickr.com/3/2603/32084013574_56c0977670_c.jpg The ones with the scale bars were from the same microscope, an...
by kinase
Mon Jan 16, 2017 3:27 pm
Forum: Pictures and Videos
Topic: Algae in fluorescence
Replies: 1
Views: 419

Algae in fluorescence

These are Guillardia theta which is entirely unrelated to Giardia lamblia. That's easy to tell if you look at the names, but hearing people pronounce the genus might lead you to believe otherwise. These have phycoerythrin. Hence when I hit them with a blue light, they fluoresce in this nice yellowis...
by kinase
Thu Nov 24, 2016 5:17 am
Forum: Microscopes and optics
Topic: AmScope T690C-PCT200-PL sufficient for spirochaete viewing?
Replies: 29
Views: 3617

Re: AmScope T690C-PCT200-PL sufficient for spirochaete viewing?

Investing a fluorescence setup just to do that is a huge waste of money, especially if it's not going to be Zeiss, Leica, Olympus, or Nikon. Where would you do this to keep it contamination free? Have you done fluoro imaging before? I don't normally use fluoro stains to look for bacteria, but I can'...
by kinase
Sat Sep 10, 2016 12:59 am
Forum: Microscopes and optics
Topic: Oiling the condenser to the slide
Replies: 11
Views: 2073

Re: Oiling the condenser to the slide

I'm not quite so sure how this is all going to work. The olympus rep said we could certainly do it with the IX73 we have but I think the PI is going to opt for trying to get the condenser for the Zeiss because that'll be way cheaper in the short run. I know one person who bought a high NA condenser ...
by kinase
Fri Sep 09, 2016 3:46 pm
Forum: Microscopes and optics
Topic: Oiling the condenser to the slide
Replies: 11
Views: 2073

Re: Oiling the condenser to the slide

Actually we got in contact with someone who has a 1.4NA condenser, even better than what I was hoping for. It's an old Zeiss inverted microscope, I think an Axiovert 100M. I have a 40x and 100x of NA~ 1.4 for it so that condenser should work great as long as it still has DIC on it. The current .5 on...
by kinase
Fri Sep 09, 2016 3:07 am
Forum: Microscopes and optics
Topic: Oiling the condenser to the slide
Replies: 11
Views: 2073

Re: Oiling the condenser to the slide

I'm having this issue. I have an oil 40x and 100x both with an NA of around 1.4 and the condensers NA is like .5. I need to see If we can get a better condenser. It's hard to find info about condensers on the manufacturers site, anybody know where they sell one? It's for very new scopes. I think I m...
by kinase
Tue Aug 30, 2016 2:39 am
Forum: Camera systems and imaging
Topic: Camera Vs Eyepiece
Replies: 31
Views: 8374

Re: Camera Vs Eyepiece

When you guys have issues, is it when trying to take photos of stuff that's moving or stuff that's still? Unless I'm doing cancer stuff, which means it's probably fluorescence and fixed and embedded in a hard setting mountant with DAPI, then it's usually stuff that's moving. I found some odd corkscr...
by kinase
Sun Aug 28, 2016 4:50 pm
Forum: Camera systems and imaging
Topic: Camera Vs Eyepiece
Replies: 31
Views: 8374

Re: Camera Vs Eyepiece

I even have this problem and I'm using this scope and this camera. I pretty much only use phase, DIC, or fluorescence. Now that I read I can see that it's not truly a 17mp camera but using pixel shifting to achieve a higher resolution. I'm truly not sure what causes it, I've tried as much optimizing...
by kinase
Thu Jul 21, 2016 1:19 am
Forum: Pictures and Videos
Topic: Quick test of new micrometer slide
Replies: 9
Views: 675

Re: Quick test of new micrometer slide

The confocal I use can put the scale bar an any other measurement you desire on the image. Usually in papers, it's just a bar that is out of the way of the subject in a corner, usually the inverse color of the background. i.e. brightfield usually has black and fluorescence with black background whit...
by kinase
Tue Jul 19, 2016 6:14 pm
Forum: Pictures and Videos
Topic: Protist storm front
Replies: 3
Views: 609

Re: Protist storm front

I don't currently have any experiments running with these, this is just a maintenance culture to keep them going until someone needs them but there was genetic analysis carried out for phylogeny purposes at some point. They're cultured as a just that ancyromonad with whatever bacteria came with the ...
by kinase
Tue Jul 19, 2016 12:48 am
Forum: Pictures and Videos
Topic: Protist storm front
Replies: 3
Views: 609

Protist storm front

Here is a culture of a bunch of ancyromonads. These are from a nikon DSLR attached to the trinocular part of a regular inverted scope, all are phase contrast. And they go in increasing magnification, with a 4x objective, then 10x, then 40x. The first image shows the central zone there being inhabite...
by kinase
Sun Jul 10, 2016 9:09 pm
Forum: Microscopy accessories
Topic: these tweezers for Insect and flower holding?
Replies: 22
Views: 3989

Re: these tweezers for Insect and flower holding?

This place has a lot of good tweezers for cheap prices. http://www.2spi.com/category/tweezers/
by kinase
Wed Jun 29, 2016 2:45 pm
Forum: Pictures and Videos
Topic: No photoshop, I swear!
Replies: 7
Views: 717

Re: No photoshop, I swear!

The laser software just lets me draw freehand lines as if you were using the line tool in paint or photoshop or gimp, holding shift lets you draw straight lines. And you can also use shape tools. I unfortunately don't have a photo of the machine but it's a Zeiss inverted scope with a PALM microbeam ...
by kinase
Wed Jun 29, 2016 3:03 am
Forum: Pictures and Videos
Topic: No photoshop, I swear!
Replies: 7
Views: 717

No photoshop, I swear!

That'd be my initials carved (not sure that's the right word) into the nucleus of a single cell. DNA is blue, a protein that localizes to sites of DNA damage caused by the laser. The laser microdissection microscope is even more precise than I thought. I actually tried this a bunch of times in a bun...
by kinase
Fri Jun 24, 2016 9:02 pm
Forum: Pictures and Videos
Topic: Small algae
Replies: 8
Views: 644

Re: Small algae

Maybe but not very green for "Green Algae". I agree. It may be because I used DIC (and, unfortunately, I didn't even look at them with BF, so I don't know what they really looked like). I think that sometimes green algae lose some of their green color (sometimes they appear brownish yellow, other t...
by kinase
Fri Jun 17, 2016 7:59 pm
Forum: Pictures and Videos
Topic: Phase images of cance cells
Replies: 5
Views: 772

Re: Phase images of cance cells

Those cells are these , metastatic lung cancer. They don't look so different from other kinds of cells because they're cancer, more because of the cell type they are. I've have bone stromal cells that are a bit longer and thinner, and also prostate cancer cells that have a more round center but thin...
by kinase
Fri Jun 17, 2016 6:28 pm
Forum: Pictures and Videos
Topic: Phase images of cance cells
Replies: 5
Views: 772

Phase images of cance cells

These were alive when I took the photo, however they weren't terribly happy. I believe they'd been out of the incubator for awhile and they've overgrown that dish. Additionally the media they were in had gone a bit too acidic and wasn't so clear, it has a pink to orange tint at this point. I wish I ...
by kinase
Mon May 30, 2016 5:15 pm
Forum: Digital processing
Topic: Why process digital images?
Replies: 14
Views: 6355

Re: Why process digital images?

I don't know why people get upset by post processing, and never think about preprocessing either. Even Old darkroom stuff had lots of burning and dodging, and even cloning in the instance of some old Nazi photos. I really miss the medium format camera I used to shoot with but the film and the develo...
by kinase
Sat May 14, 2016 7:07 pm
Forum: Pictures and Videos
Topic: Some more lung cancer cells, nyquist sampling
Replies: 4
Views: 443

Re: Some more lung cancer cells, nyquist sampling

I just did an antibody dilution test yesterday, the slide's curing right now. It was a chambered slide with 8 wells where on the top row I held the secondary antibody concentration constant and changed the primary, and the bottom row was the reverse of that. I'll have a chance to image it on a confo...
by kinase
Fri May 13, 2016 8:49 pm
Forum: Pictures and Videos
Topic: what is this
Replies: 1
Views: 556

Re: what is this

Probably just stuff moving due to Brownian motion, either it was on the slide before you put the sample on, on the coverslip, or if you added water it'd have been in there or from somewhere else. It'd be pretty hard to keep out absolutely everything from getting on your slide in your house.
by kinase
Fri May 13, 2016 1:46 am
Forum: Pictures and Videos
Topic: Some more lung cancer cells, nyquist sampling
Replies: 4
Views: 443

Some more lung cancer cells, nyquist sampling

Someone from Nikon stopped by to help us install an incubation unit on our confocal scope. Someone from a different lab brought a GFP tagged tubulin cell line with him to come give it a test and we had a look. I watched tubulin moving around in a cell in real time at a very fast frame rate in 3D. Ne...
by kinase
Tue May 10, 2016 3:48 pm
Forum: Specimens, samples and slides
Topic: Using low temperatures to fix samples
Replies: 1
Views: 1013

Using low temperatures to fix samples

There's one particular algae I have that doesn't take so well to formaldehyde, glutaraldehyde, or any other fixing agent I've tried. They usually just explode when I add anything. So far I've been able to stun them with nickel sulfate but that irritates them a bit too. Has anybody tried just throwin...
by kinase
Tue May 03, 2016 1:04 pm
Forum: Beginner's corner
Topic: Microscopy with Nikon D810
Replies: 11
Views: 1875

Re: Microscopy with Nikon D810

Thanks for the reply. It is a fairly high end camera. I thought this would be more like fitting a low end lens to my camera, which I've done before. That's pretty much what's going to happen, the microscope is going to be your limiting factor here. If you want to do some microscopy and don't want t...