The patch is na specific but the aperture width effects the contrast.
A narrow gap of around 1mm will improve the resolution.
Search found 608 matches
- Mon Apr 22, 2024 4:54 am
- Forum: Illumination Techniques
- Topic: Darkfield Illumination
- Replies: 17
- Views: 1119
- Fri Apr 05, 2024 5:46 am
- Forum: Microscopes and optics
- Topic: Cleaning Fungus from Microscope Optics
- Replies: 5
- Views: 943
Re: Cleaning Fungus from Microscope Optics
Zeiss coated optics cleaner spray.
For when you assume the coating is damaged after isopropyl meths soapy didn't work.
For when you assume the coating is damaged after isopropyl meths soapy didn't work.
- Wed Apr 03, 2024 8:19 am
- Forum: Microscopes and optics
- Topic: Averaging condenser and objective numerical aperture when predicting resolution
- Replies: 49
- Views: 5302
Re: Averaging condenser and objective numerical aperture when predicting resolution
Vintage diatom slides are generally not expensive.
SEM images will help to determine if you are seeing aberrations or resolved structures.
SEM images will help to determine if you are seeing aberrations or resolved structures.
- Fri Mar 29, 2024 1:25 pm
- Forum: My microscope
- Topic: Euromex Microscopes?
- Replies: 5
- Views: 2994
Re: Euromex Microscopes?
It's a subject covered here.
I'd value a PL/PH trinoc iscope at £500 boxed in as new condition.
LED is good for not boiling live subjects.
I'd value a PL/PH trinoc iscope at £500 boxed in as new condition.
LED is good for not boiling live subjects.
- Thu Mar 28, 2024 9:07 pm
- Forum: My microscope
- Topic: Euromex Microscopes?
- Replies: 5
- Views: 2994
Re: Euromex Microscopes?
I have an iscope pH pl trinocular. The slide clamp is junk. The auto power off isn't true. Apparently the fine focus can work loose but can be fixed. Is it worth the new price? No. It's not Kohler, it's a diffused 3w neo LED with a field iris. The objectives look similar to motic but they are not th...
- Sat Mar 16, 2024 6:58 am
- Forum: Illumination Techniques
- Topic: BH-2 and darkfield
- Replies: 2
- Views: 2693
Re: BH-2 and darkfield
Low power condensers are the least suited to DF. Dry will be limited to 0.85 realistically but 0.75 is a more likely limit for 1.25. Oil would be for 0.5 to 0.9,lower na objectives will only have the centre illuminated. There are exceptions as always. If you have a phase telescope and a slide ringin...
- Thu Mar 14, 2024 10:28 pm
- Forum: Microscopy accessories
- Topic: 1/4 wave filter source
- Replies: 13
- Views: 2767
Re: 1/4 wave filter source
I've only seen bonded to make a single element filter.
- Fri Mar 08, 2024 9:52 pm
- Forum: Illumination Techniques
- Topic: Condenser Alignment.
- Replies: 17
- Views: 2544
Re: Condenser Alignment.
Think you'll find the yellow and blue fringing is how the subject edges are illuminated,the objective can't do a busting lot about it.
- Fri Mar 08, 2024 7:16 pm
- Forum: Illumination Techniques
- Topic: Condenser Alignment.
- Replies: 17
- Views: 2544
Re: Condenser Alignment.
But I do notice a small coloration off to one side of field dia. during Kohler alignment. This is more obvious when condenser is oiled which minimizes reflections between condenser and slide. I'll have to examine this later thanks to your post. Thanks, Glenn Likewise, I never tried centration of th...
- Wed Feb 28, 2024 10:57 am
- Forum: Beginner's corner
- Topic: New Technique
- Replies: 4
- Views: 1369
Re: New Technique
G4 sockets allow for error in filament placement. Custom bulbs with a collar require a jig to focus the collar fitment precisely. Probably not a good idea to run 6v 30w on a supply intended for 20w. 6v @ 30w is 5 amps. 6v @ 20w is 3.5 amps. Either the voltage will be low and give a warm tint or the ...
- Fri Feb 23, 2024 3:56 pm
- Forum: Home-made microscope adaptations
- Topic: How to Diy objective parcentering.
- Replies: 6
- Views: 1665
Re: How to Diy objective parcentering.
Another great contribution thanks. Striving for perfection in production is the biggest waste of time and money resulting in bankruptcy or buy out. There is an agreed standard, sometimes that is exceeded by chance. Below that is usually left for the phase B tester to dispute and resolve. The intende...
- Wed Feb 21, 2024 7:56 am
- Forum: Introduce yourself
- Topic: Never too late
- Replies: 2
- Views: 2102
Re: Never too late
Happy days.
We have Watson and V***ers in the UK.
It's a slippery slope if you don't have a lot of space, the Watson microsystem 70 cases are designed to be stacked.
Good luck from sunny Devon.
We have Watson and V***ers in the UK.
It's a slippery slope if you don't have a lot of space, the Watson microsystem 70 cases are designed to be stacked.
Good luck from sunny Devon.
- Tue Feb 20, 2024 3:26 pm
- Forum: Microscopes and optics
- Topic: Is it ok to use metalloscopy objective in biology microscope?
- Replies: 8
- Views: 1193
Re: Is it ok to use metalloscopy objective in biology microscope?
Optimised for epi illumination?
What 10x do you have?
What 10x do you have?
- Tue Feb 06, 2024 9:03 am
- Forum: Pictures and Videos
- Topic: Collection of live round Diatoms
- Replies: 2
- Views: 426
Re: Collection of live round Diatoms
Excellent, thanks for sharing your find.
- Fri Feb 02, 2024 4:11 pm
- Forum: Illumination Techniques
- Topic: Has anyone ever used LWD condenser?
- Replies: 8
- Views: 1978
Re: Has anyone ever used LWD condenser?
More useful for inverted scope.
Would work ok for lower na, maybe 2x.
Would work ok for lower na, maybe 2x.
- Wed Jan 31, 2024 10:54 am
- Forum: Microscopes and optics
- Topic: Which 40x and 4x objectives for Nikon Labophot?
- Replies: 16
- Views: 1529
Re: Which 40x and 4x objectives for Nikon Labophot?
The light source is the cause. LED systems with pretend Kohler will have similar aberrations. It's just the way things are. The objective can't fix the problem. It could be cost effective to pick up a Labophot 2 with a dead PSU but working fine focus and add a variable 12v 5A DC supply. There are 2 ...
- Tue Jan 30, 2024 12:42 pm
- Forum: Microscopes and optics
- Topic: Which 40x and 4x objectives for Nikon Labophot?
- Replies: 16
- Views: 1529
Re: Which 40x and 4x objectives for Nikon Labophot?
Close and focus on the Kohler iris. There will be coloured edges. LED is the cause not the objective. There are but the colours vary according to the objective. I can almost get rid of the edges when everything is properly aligned, especially with the Plan Apo and Fluor objectives. 100% what I woul...
- Mon Jan 29, 2024 7:40 pm
- Forum: Microscopes and optics
- Topic: Which 40x and 4x objectives for Nikon Labophot?
- Replies: 16
- Views: 1529
Re: Which 40x and 4x objectives for Nikon Labophot?
Close and focus on the Kohler iris.
There will be coloured edges.
LED is the cause not the objective.
There will be coloured edges.
LED is the cause not the objective.
- Mon Jan 29, 2024 10:14 am
- Forum: Microscopes and optics
- Topic: Which 40x and 4x objectives for Nikon Labophot?
- Replies: 16
- Views: 1529
Re: Which 40x and 4x objectives for Nikon Labophot?
If you like yellow and blue fringing LED is an upgrade.
- Sun Jan 28, 2024 10:05 pm
- Forum: Microscopes and optics
- Topic: Which 40x and 4x objectives for Nikon Labophot?
- Replies: 16
- Views: 1529
Re: Which 40x and 4x objectives for Nikon Labophot?
Something is out of alignment.
Wrong bulb or LED "upgrade".
Unlikely to be the objectives.
Wrong bulb or LED "upgrade".
Unlikely to be the objectives.
- Tue Jan 23, 2024 4:58 pm
- Forum: Microscopes and optics
- Topic: DIC for birefringent objects
- Replies: 9
- Views: 1003
Re: DIC for birefringent objects
I'd wonder about the mountant being an issue.
- Thu Jan 18, 2024 11:50 am
- Forum: Microscopes and optics
- Topic: Has anyone compared Chinese objectives to similar objectives from the Big Four?
- Replies: 3
- Views: 834
Re: Has anyone compared Chinese objectives to similar objectives from the Big Four?
More than anything if the slide clamp is up to holding a slide and the plastic mechanical parts hold up.
There's the yellow and blue fringing from the pretend Kohler and LED to deal with.
The objective can only do so much.
There's the yellow and blue fringing from the pretend Kohler and LED to deal with.
The objective can only do so much.
- Thu Jan 11, 2024 10:01 pm
- Forum: Pictures and Videos
- Topic: Fossil diatom from Oamaru
- Replies: 33
- Views: 34328
Re: Fossil diatom from Oamaru
Sticking with 275.
- Sun Jan 07, 2024 7:50 am
- Forum: Beginner's corner
- Topic: Inverted scope
- Replies: 14
- Views: 14178
Re: Inverted scope
Best avoid anything that looks like it's already lived in marine biology labs.
Good luck with finding a bargain, they are available.
Good luck with finding a bargain, they are available.
- Sat Jan 06, 2024 12:14 pm
- Forum: Beginner's corner
- Topic: Inverted scope
- Replies: 14
- Views: 14178
Re: Inverted scope
The biggest difference is the cleaning of samples. Residues will quickly settle Infront of the subjects. Settling and rinsing may or may not be interesting. DF is more challenging but what is life without taking some on. You should get a lot for the equivalent budget. I don't use mine much but would...
- Fri Dec 22, 2023 12:48 pm
- Forum: Beginner's corner
- Topic: Tools for dissecting really tiny spiders and insects
- Replies: 6
- Views: 4250
Re: Tools for dissecting really tiny spiders and insects
000 sable brushes are worth trying for moving things with minimal damage.
- Wed Dec 20, 2023 9:28 am
- Forum: Microscopes and optics
- Topic: Thoughts on these microscopes for sale on Facebook appreciated
- Replies: 7
- Views: 7896
Re: Thoughts on these microscopes for sale on Facebook appreciated
Definitely a junk pile of projects.
If the labophot 2 PSU is working it may be viable, Labophot 2 complete with failed PSU are cheap and abundant.
If the labophot 2 PSU is working it may be viable, Labophot 2 complete with failed PSU are cheap and abundant.
- Thu Dec 14, 2023 8:42 pm
- Forum: Pictures and Videos
- Topic: Fossil diatom from Oamaru
- Replies: 33
- Views: 34328
Re: Fossil diatom from Oamaru
I'll kick off with 275 microns ish across the round diatom.
- Tue Dec 12, 2023 7:31 pm
- Forum: Beginner's corner
- Topic: Question Regarding Darkfield Condensers
- Replies: 39
- Views: 117787
Re: Question Regarding Darkfield Condensers
I'm confident you will enjoy the DOF from x40 .65 far more than the resolution at the limits.
X20 .5 is great when you train yourself to see what is there.
Get some bargain diatom strew slides from eBay and just have fun with it.
X20 .5 is great when you train yourself to see what is there.
Get some bargain diatom strew slides from eBay and just have fun with it.
- Tue Dec 12, 2023 2:50 pm
- Forum: Beginner's corner
- Topic: Question Regarding Darkfield Condensers
- Replies: 39
- Views: 117787
Re: Question Regarding Darkfield Condensers
How long is the lag from posting here to when it appears on their site?