Yeah, as long as you also use finite objectives and make sure the mechanical tube length is exactly 160mm.
The real question is "why"? I have seen it being done before, but it was rather opportunistic than necessity.
Search found 119 matches
- Fri May 03, 2024 1:00 pm
- Forum: Microscopes and optics
- Topic: Older viewing heads on newer bodies
- Replies: 3
- Views: 186
- Fri May 03, 2024 12:57 pm
- Forum: Illumination Techniques
- Topic: A modern take on Koehler
- Replies: 4
- Views: 639
Re: A modern take on Koehler
Although I still commend the paper to anyone with an interest in Koehler illumination … I am rather bewildered by the assertion that it is low-cost Yeah, it is low-cost. Any proper lab will already have sorted cabinets filled with cage components, so those are "free". The lenses would be another $2...
- Tue Apr 23, 2024 1:33 am
- Forum: Illumination Techniques
- Topic: Prepared slide set for demonstrating contrast techniques?
- Replies: 8
- Views: 746
Re: Prepared slide set for demonstrating contrast techniques?
A slide of fluorescence beads and a stage ruler will work decently for it all. You can use the beads to demonstrate BF vs phase/DIC, because under BF, they are hardly discernable.
Any petrographic slide for polarised light.
Blood smears for phase, DIC and oblique.
Any petrographic slide for polarised light.
Blood smears for phase, DIC and oblique.
- Thu Apr 11, 2024 5:51 am
- Forum: Microscopes and optics
- Topic: Averaging condenser and objective numerical aperture when predicting resolution
- Replies: 49
- Views: 5483
Re: Averaging condenser and objective numerical aperture when predicting resolution
Diatoms are adequate, but not quantitative. It fails as a decent reference and vintage slides were prepared with vintage techniques, making them somewhat unsuitable. As a general toll, sure, it works well enough. I love diatoms, I have invested lots of time and money in them but the idea of test dia...
- Thu Apr 11, 2024 5:40 am
- Forum: Beginner's corner
- Topic: retrieve specific specimens
- Replies: 8
- Views: 775
Re: retrieve specific specimens
No offence, just practice more. Do you use chopsticks? Pinch the pipette between two fingers and use your dominant hand to manipulate the pipette with the other being stationary. Do this under a stereomicroscope. This works very well for larger specimens. For smaller ones, you need something more el...
- Wed Apr 03, 2024 4:35 am
- Forum: For forum members who want to buy and sell equipment
- Topic: Olympus BX items for sale
- Replies: 4
- Views: 1382
Re: Olympus BX items for sale
I am interested in the u-dic60. I will see if I can find a u-dic20 for the swap.
- Wed Apr 03, 2024 4:34 am
- Forum: For forum members who want to buy and sell equipment
- Topic: Searching For: Extention between intermediate tube and the observation tube for an IMT-2 Olympus Inverted Microscope
- Replies: 4
- Views: 935
Re: Searching For: Extention between intermediate tube and the observation tube for an IMT-2 Olympus Inverted Microscope
Any Olympus tube that houses correction lenses for the extension should work. The infinite versions are just mechanical raisers without correction optics, it could work but it will probably affect the viewing. Or I suppose you can DIY a raiser and use longer tube objectives, like 210 instead of 160.
- Wed Apr 03, 2024 4:29 am
- Forum: Microscopes and optics
- Topic: Averaging condenser and objective numerical aperture when predicting resolution
- Replies: 49
- Views: 5483
Re: Averaging condenser and objective numerical aperture when predicting resolution
There seemed to be some confusion on this in the papers and other stuff I looked at, but generally as far as I understood the averaging rule (especially if used as an approximation for real-world specimens) is only supposed to apply if NA_cond <= NA_obj. Yeah, that is where it falls short as a gene...
- Wed Apr 03, 2024 4:17 am
- Forum: Microscopes and optics
- Topic: Olympus BX60 3D model
- Replies: 1
- Views: 2348
Re: Olympus BX60 3D model
Rule number one to transporting a microscope is full disassembly, otherwise it will do so by itself and you would have a bad day. You are better off purchasing a large Pelican case with pick and pluck foam.
- Wed Apr 03, 2024 4:07 am
- Forum: Microscopes and optics
- Topic: Olympus X-Line Objectives
- Replies: 6
- Views: 1875
Re: Olympus X-Line Objectives
Evident is ultimately a Japanese company. Being a Japanese company, they suffer from all the shortcomings of Japanese company culture, no offence, I have worked for one and with many. They have tunnel vision, general stubbornness and simply cannot understand the western market. We often have to make...
- Wed Apr 03, 2024 3:14 am
- Forum: Microscopes and optics
- Topic: Averaging condenser and objective numerical aperture when predicting resolution
- Replies: 49
- Views: 5483
Re: Averaging condenser and objective numerical aperture when predicting resolution
I personally refuse to use this formula simply because a 1.4 NA Apl-Achr condenser fully open coupled with a 4x at 0.1 NA will not yield 0.75 NA. You will be better off using diatoms as resolution targets.
- Mon Mar 25, 2024 4:32 am
- Forum: Microscopes and optics
- Topic: Do I need another objective?
- Replies: 61
- Views: 7958
Re: Do I need another objective?
I will add one of those $120 PlanApo 20x objectives, the ones with branding.
Also hunt for a 10x cheap fluorite and 4x achromatic to use as finders.
Also hunt for a 10x cheap fluorite and 4x achromatic to use as finders.
- Wed Mar 20, 2024 1:13 am
- Forum: Microscopy accessories
- Topic: 1/4 wave filter source
- Replies: 13
- Views: 2864
Re: 1/4 wave filter source
The one I pulled apart must be very cheap or very old then, unfortunate. I have seen film type waveplates for less than $20 for a 50x50mm square. Supposedly they are used for screens.
- Thu Mar 14, 2024 5:08 am
- Forum: Microscopy accessories
- Topic: 1/4 wave filter source
- Replies: 13
- Views: 2864
Re: 1/4 wave filter source
A circular polariser consists of a 1/4 λ plate and a linear polariser. If you have one lying around, take it apart and you have your waveplate with an additional linear polariser.
- Thu Mar 07, 2024 4:12 am
- Forum: Microscopes and optics
- Topic: Olympus CX43 any thoughts ,made in China apparently
- Replies: 19
- Views: 1753
Re: Olympus CX43 any thoughts ,made in China apparently
The modern CX3 line are all mostly made in china, that does not mean a decrease in quality. As products go, you usually get what you paid for. They are still quite expensive, no more or less when they were produced in Japan. I cannot imagine a cx43 with phase contrast selling for similar prices to a...
- Thu Mar 07, 2024 3:59 am
- Forum: My microscope
- Topic: My old 70s Japanese Denkar microscope
- Replies: 5
- Views: 2091
Re: My old 70s Japanese Denkar microscope
Denkar was one of the many many white labelled Japanese optical products, others include Kenko, Sumo, Tasco, KKK-kogaku (yes, really :roll: ) and so on. Unless it has a made in Japan signage, you can probably assume it came from one of the few Chinese optics factories. The famous Swift field microsc...
- Mon Mar 04, 2024 4:32 am
- Forum: Camera systems and imaging
- Topic: How do you deal with the glare in camera
- Replies: 8
- Views: 2735
Re: How do you deal with the glare in camera
Stick them into your tube.
- Mon Mar 04, 2024 4:24 am
- Forum: Microscopes and optics
- Topic: Microscope storage/portability options
- Replies: 4
- Views: 1069
Re: Microscope storage/portability options
I transported half a dozen with a commercial removalist, no damage. All scopes broken down. I sourced original boxes from Evident, when possible. For critical parts such as objectives and condensers, I did it myself with a Pelican case. Key is complete removal of all user-removable parts, otherwise,...
- Mon Mar 04, 2024 4:21 am
- Forum: Microscopy accessories
- Topic: Mystery Nikon Magnifier
- Replies: 8
- Views: 2147
Re: Mystery Nikon Magnifier
As originally stated: although the lower lens element works as a very nice ‘magnifying glass’ … I haven’t found any setting where the complete device will achieve focus ! … from which I would deduce that it is intended to work in conjunction with other optics, rather than just being a loupe. Michae...
- Mon Mar 04, 2024 4:17 am
- Forum: Microscopes and optics
- Topic: Olympus BH2 versus BX series..how does one decide?
- Replies: 6
- Views: 1164
Re: Olympus BH2 versus BX series..how does one decide?
It depends on what you want. If you want a first scope to mess around and then sell at a minimal loss, or even a small profit, the BH2 is pretty good. If you want a petrography microscope, I would personally choose the BH2 because parts are cheaper. A BX petrographic microscope would run you half a ...
- Tue Feb 20, 2024 2:58 am
- Forum: Microscopy accessories
- Topic: Olympus BH2 Magnification Changer
- Replies: 4
- Views: 1552
- Tue Feb 20, 2024 2:39 am
- Forum: My microscope
- Topic: как устроен механический тубус ortholux2 как можно перевести на бесконечную оптику,
- Replies: 5
- Views: 2615
Re: как устроен механический тубус ortholux2 как можно перевести на бесконечную оптику,
I don't think it is possible to change a finite optics microscope to infinity-corrected. You would be better off getting higher-grade finite optics for your Ortholux 2. Main advantage of infinity systems is ability to insert additional attachments into infinity space. Being a beginner, it is not so...
- Tue Feb 20, 2024 2:30 am
- Forum: My microscope
- Topic: Does Anybody Recognize this Inverted 'Scope?
- Replies: 33
- Views: 7029
Re: Does Anybody Recognize this Inverted 'Scope?
The logo says 上海 which means Shanghai, likely it came from one of the three or four state-run optics enterprises back in the days. It does not indicate which factory, but perhaps a nameplate at the back would say something like 上海光學三廠 (Shanghai optics Co 3rd factory), indicating its origin. I own a ...
- Tue Jan 23, 2024 3:19 am
- Forum: Microscopy accessories
- Topic: Mystery Nikon Magnifier
- Replies: 8
- Views: 2147
Re: Mystery Nikon Magnifier
Fine focus assist to be used with a camera mount apparatus that had its own eyepiece, it mounts onto the eyepiece.
- Wed Jan 10, 2024 4:33 am
- Forum: Microscopes and optics
- Topic: Olympus UPlanAPO 40x/1.00 Oil iris?
- Replies: 6
- Views: 7154
Re: Olympus UPlanAPO 40x/1.00 Oil iris?
1. Higher RI so that NA 1 oil will be brighter than an NA 0.95 dry, most immersion oil RI ~ 1.52 (e-line), air ~1.00. This means a brighter fluorescence signal and better matched RI to the glass slip. 2. The 1.0 oil one does not care about the thickness of your coverslip (well unless it is thicker t...
- Wed Jan 10, 2024 4:20 am
- Forum: Microscopes and optics
- Topic: Correction Collar : Leitz [surprise]
- Replies: 11
- Views: 13163
Re: Correction Collar : Leitz [surprise]
That is totally BS, the effect can be demonstrated easily and 0.7 NA is already high enough to see the adverse effects of spherical aberration. Zeiss even made spehrical aberration compensation slides with a glass wedge. Either during that time, imaging systems were lacklustre and thus the effect wa...
- Wed Jan 10, 2024 4:12 am
- Forum: Illumination Techniques
- Topic: Brightfield cube in U-RFAA (Olympus), compatible with DICR?
- Replies: 3
- Views: 6308
Re: Brightfield cube in U-RFAA (Olympus), compatible with DICR?
1. Works 2. Why change to Halogen? PE-300 would work well. 3. No, unless you only want the polarisers always crossed which limits functionality. -1: one rotatable analyser, no passthrough slot -2: with passthrough slot -3: same as above but there are lots of fakes from a Nanjing factory flooding the...
- Tue Jan 09, 2024 7:05 am
- Forum: Beginner's corner
- Topic: Inverted scope
- Replies: 14
- Views: 14221
Re: Inverted scope
There's gotta be something to that configuration for Nikon to build their entire super-resolution line around it, in addition to their line-up of conventional inverted scopes . All of these techniques are available for upright as well. Nikon like the other 3 are selling their top-line inverted micr...
- Thu Dec 28, 2023 2:47 pm
- Forum: Pictures and Videos
- Topic: Hela Cells fluorescence DIC
- Replies: 2
- Views: 1677
Hela Cells fluorescence DIC
40x.
Nucles stained with DAPI.
- Mon Dec 25, 2023 6:54 am
- Forum: Illumination Techniques
- Topic: Does phase contrast make sense for its price?
- Replies: 49
- Views: 35085
Re: Does phase contrast make sense for its price?
Thanks. I'm a bit confused: Get an abbe condenser which allows inserts to be placed at the back focal plane of the objective? My understanding is, I need to DYI a slide like the picture above, and also need to insert a varel ring at the back focal plane of the objective, right? The varel ring is ki...