Search found 309 matches

by wporter
Sun May 31, 2020 7:51 pm
Forum: Microscopes and optics
Topic: Breaking the baffling B & L Balplan buffalo.
Replies: 38
Views: 3175

Re: Breaking the baffling B & L Balplan buffalo.

No, coatings from the 60's should be immune to any of those solvents, being primarily magnesium fluoride. (As for modern multicoatings such as are found on camera lenses, the types of coatings differ, so opinions and results differ, so it's best not to chance certain solvents on those. However, it's...
by wporter
Tue May 26, 2020 9:54 pm
Forum: Articles and images to be published
Topic: Making Permanent Microscope Slides of Insects
Replies: 3
Views: 94

Re: Making Permanent Microscope Slides of Insects

Insect Microscopy, by Chick, has quite a bit on making insect slides.
by wporter
Tue May 26, 2020 4:44 pm
Forum: Beginner's corner
Topic: Too small?
Replies: 8
Views: 205

Re: Too small?

the ebola virus is 600 - 1400 nm . Could it be seen with a light microscope? If so, at what magnitude? If not, other bacteria would be too small for the light microscope, wouldn't they? To clarify for the OP, actually, using darkfield, they might be 'seen', although maybe not very recognizable or i...
by wporter
Sun May 24, 2020 11:55 pm
Forum: Microscopes and optics
Topic: Low-cost epi-illumination, 4 techniques in one
Replies: 1
Views: 102

Low-cost epi-illumination, 4 techniques in one

This 2016 paper makes for interesting reading:

A Simple low-cost device enables four epi-illumination techniques on standard light microscopes
by Robert R. Ishmukhametov, Aidan N. Russell, Richard J. Wheeler, Ashley L. Nord & Richard M. Berry


https://www.nature.com/articles/srep20729
by wporter
Sat May 23, 2020 2:51 am
Forum: Specimens, samples and slides
Topic: reflected light silicon chip die
Replies: 39
Views: 9092

Re: reflected light silicon chip die

MichaelG, forgot to add that you may have one of those chips that has a coating. PeteM alerted me as to the existence of those; that could seriously mess up the microscopy!
by wporter
Sat May 23, 2020 2:47 am
Forum: Specimens, samples and slides
Topic: reflected light silicon chip die
Replies: 39
Views: 9092

Re: reflected light silicon chip die

Here is a similar chip imaged episcopically with my Univar, in darkfield, brightfield, BF with the epi condenser offset a bit (two of those shots) and phase, all with the 50x Reichert Plan Fluor objective.

I'll try this on a Balplan and a Zeiss Universal when I get a chance.
by wporter
Thu May 21, 2020 5:57 pm
Forum: Microscopes and optics
Topic: Evaluating potential microscope objective upgrades.
Replies: 22
Views: 353

Re: Evaluating potential microscope objective upgrades.

I'm not Stephen, but the translation might be:

x-axis: distance from the optical axis, mm

y-axis: % chromatic aberration in the intermediate image with 20mm

Graph title: Presentation of the different chromatic aberration corrections
by wporter
Tue May 19, 2020 6:42 pm
Forum: Microscopes and optics
Topic: Best way to assess condition of microscope objective optics?
Replies: 8
Views: 180

Re: Best way to assess condition of microscope objective optics?

If one doesn't have a stereo microscope or a phase telescope, are there any techniques that can be used to evaluate objectives? A 10x hand lens is probably the best next choice. Hold the hand lens in one place close to your eye and move the objective towards and away from you to focus on different ...
by wporter
Tue May 12, 2020 6:03 pm
Forum: Miscellaneous
Topic: The Breadth of Function in One-Celled Animals
Replies: 10
Views: 515

Re: The Breadth of Function in One-Celled Animals

Unfortunately, the article is behind a paywall, for $6, and your local library, like mine, may be closed for a while, but here's a link to a digital collector's edition with that article, plus more great ones, for not much more $: https://www.scientificamerican.com/magazine/special-editions/2018/spe...
by wporter
Tue May 12, 2020 12:42 am
Forum: Miscellaneous
Topic: The Breadth of Function in One-Celled Animals
Replies: 10
Views: 515

Re: The Breadth of Function in One-Celled Animals

Could one of our cells, any cells perform as many functions as the protozoan potentially? That is like asking "is a Swiss Army knife capable of more than a hammer?" Sure, but if the hammer always comes with a tool box full of other specialized tools, the toolbox can equal the Swiss Army knife, and ...
by wporter
Mon May 11, 2020 1:19 am
Forum: Miscellaneous
Topic: The Breadth of Function in One-Celled Animals
Replies: 10
Views: 515

Re: The Breadth of Function in One-Celled Animals

It is only a big mystery because we haven't figured it all out, yet. But basically it is as was said: a unicellular organism (UO) makes use of internal funtional parts to do the same functions as our organs and supportive structures. Both are complex systems, making use of positive and negative feed...
by wporter
Fri May 08, 2020 3:40 am
Forum: Miscellaneous
Topic: PVC and PET microplastics in caddisfly
Replies: 6
Views: 702

Re: PVC and PET microplastics in caddisfly

many new sanitizers and sterilizers will emerge in the near future
A recent suggestion is to inject them directly into your veins. Then you don't have to worry about it anymore.
by wporter
Tue May 05, 2020 1:08 am
Forum: Pictures and Videos
Topic: Epi-Fluorescence Microscopy and Deconvolution
Replies: 12
Views: 1504

Re: Epi-Fluorescence Microscopy and Deconvolution

There is another deconvolution program called Focus Magic, which has a PS plugin, although the plugin may differ somewhat from the standalone version. There is a free trial. It also does de-motion-blurring. I have used it a bit in astrophotography. [Edit:] I forgot to say that it is a Windows progra...
by wporter
Sun May 03, 2020 1:31 am
Forum: Illumination Techniques
Topic: Leica Integrated Modulation Contrast (Variant of Hoffman)
Replies: 7
Views: 868

Re: Leica Integrated Modulation Contrast (Variant of Hoffman)

Whoops, I had it wrong about the small ND filter part of the smaller slider; it is actually a glass disk that covers the entire hole. On it are plated the areas of 15% and 1%, the rest left clear. I can't say about your microscope. What make and model is it? What is the triangular filter for? edit: ...
by wporter
Sat May 02, 2020 7:45 pm
Forum: Illumination Techniques
Topic: Improving Top-Stage Lighting Using a Smaller Aperture on the Lamp
Replies: 22
Views: 1478

Re: Improving Top-Stage Lighting Using a Smaller Aperture on the Lamp

Your narrow-beam images are definitely sharper, I think simply from the more point-source lighting providing more shadows, and thus a crisper relief-effect; whereas with a broader source, the shadows are eliminated from getting illuminated from many different points, and things are washed out.
by wporter
Sat May 02, 2020 6:55 pm
Forum: Illumination Techniques
Topic: Leica Integrated Modulation Contrast (Variant of Hoffman)
Replies: 7
Views: 868

Re: Leica Integrated Modulation Contrast (Variant of Hoffman)

Unlike the Leica website, that misrepresents this particular IMC set in fig 5, this ebay set has at least two components, the small slider and the big slider. The big slider also has two openings, an open one for brightfield, and an IMC one that has a variable slit, with the slit adjuster labeled fo...
by wporter
Fri May 01, 2020 10:41 pm
Forum: Beginner's corner
Topic: Specimen ethics
Replies: 42
Views: 3284

Re: Specimen ethics

A big reason why isopropyl alcohol should be kept in a tightly closed container is that it is very hygroscopic, at least until it dilutes itself down to 65% (35% water).
by wporter
Tue Apr 28, 2020 7:40 am
Forum: Beginner's corner
Topic: Need advice buying a Reichert Microstar IV
Replies: 23
Views: 1716

Re: Need advice buying a Reichert Microstar IV

75RR, I agree with everything you have laid out about ebay descriptive terms. However, I do think that necessarily ascribing nasty motives to a vendor (i.e., 'playing' you) is often inaccurate. Many of these mass vendors of used equipment may just be covering themselves against over-representing the...
by wporter
Tue Apr 28, 2020 3:38 am
Forum: Beginner's corner
Topic: Need advice buying a Reichert Microstar IV
Replies: 23
Views: 1716

Re: Need advice buying a Reichert Microstar IV

For the neophytes reading the above, I second Apo's analysis of ebay returns. I have had very good luck with 'no returns' in getting a refund and/or free ship-back. In many cases, the seller gave a refund and said 'just keep it, no problem', not wanting to go through the headache of putting it back ...
by wporter
Fri Apr 24, 2020 11:44 pm
Forum: Beginner's corner
Topic: Specimen ethics
Replies: 42
Views: 3284

Re: Specimen ethics

Perhaps part of the cognitive disconnect that raises questions like this, i.e., I feel sorry for my microbes on my slide, I hate to hurt them, is it ok to flush them, etc., etc., arises from the mere fact that when examined under the microscope, the apparent size of the microlife is very macro, and ...
by wporter
Sat Apr 18, 2020 6:12 pm
Forum: Beginner's corner
Topic: Need advice buying a Reichert Microstar IV
Replies: 23
Views: 1716

Re: Need advice buying a Reichert Microstar IV

A great clinical/lab microscope, of an era between the older heavy brass-and-steel beauties, and the modern stylish-but-plastic Chinese-made types. Some of the best planachro optics out there. Look for one with: 1) full complement of objectives, and a couple of eyepieces, 2) smooth focusing througho...
by wporter
Fri Apr 17, 2020 12:25 am
Forum: Microscopes and optics
Topic: objectives labeled DIC
Replies: 2
Views: 206

Re: objectives labeled DIC

1. No. Yes. 2. No. 3. It is labeled DIC because it is a strain-free, i.e., won't exhibit any colors when examined with polarized light; obviously that would interfere with DIC or polarization effects when using those techniques. Strain-free was originally required for polarization techniques, e.g., ...
by wporter
Wed Apr 15, 2020 5:40 pm
Forum: Camera systems and imaging
Topic: Sony APS-C on Swift Trinocular
Replies: 7
Views: 452

Re: Sony APS-C on Swift Trinocular

+1, Apo. Well stated.
by wporter
Sun Apr 12, 2020 6:41 pm
Forum: Introduce yourself
Topic: Adding / requiring member locations?
Replies: 15
Views: 1006

Re: Adding / requiring member locations?

I certainly can appreciate both perspectives: the desire for some anonymity on the part of the poster, yet the frustration of readers who wonder "where the heck are you posting from?" Many posters, especially neophytes who are asking for advice on a possible purchase, are strongly encouraged to add ...
by wporter
Sun Mar 22, 2020 12:06 am
Forum: Camera systems and imaging
Topic: Olympus BHS vs modern microscope
Replies: 8
Views: 1869

Re: Olympus BHS vs modern microscope

Most comparisons, very much such as the one you linked to, are specious, and are generally poorly done. It is most always an apples-to-oranges comparison: the N.A.s are not the same; the focus is on different points of the specimen for the different lenses; the magnifications are not the same; the p...
by wporter
Fri Feb 28, 2020 4:46 pm
Forum: Microscopes and optics
Topic: Leica Reichert Jung Polyvar (models)
Replies: 46
Views: 15616

Re: Leica Reichert Jung Polyvar (models)

Member wporter is in the midst of a move and does not have access to documentation, nor his Reichert objectives, so he's not much help. My scope is a Univar, but uses some of the same optics as the Polyvar Met. But I will say that the Plan Fluors go down at least to 5x, although quite frankly, at th...
by wporter
Mon Feb 10, 2020 5:54 pm
Forum: Beginner's corner
Topic: DNA?
Replies: 7
Views: 3388

Re: DNA?

DNA is visible with a 100x objective, but it is all coiled up (with the coils coiled, etc.) into a chromosome. So you will not be able to see the actual double-helix of DNA, just the giant hyper-organized wad that is the chromosome: a reduction in length from the meter(s)-long DNA strand into a chro...
by wporter
Mon Feb 10, 2020 5:35 pm
Forum: Microscopes and optics
Topic: B&L (or Cambridge) PhotoZoom inverted microscope?
Replies: 5
Views: 3619

Re: B&L (or Cambridge) PhotoZoom inverted microscope?

The ornamental design is downloadable from here (USD308879, 1990): http://www.freepatentsonline.com/D308879.html The rubber objective turret cover patent (!) along with a lot more (!?), is detailed here (US 4,770,520, 1988): http://www.freepatentsonline.com/4770520.html Why the patent 4,770,520 has ...
by wporter
Mon Feb 10, 2020 12:08 am
Forum: Microscopes and optics
Topic: 23.2 vs 30mm
Replies: 38
Views: 7914

Re: 23.2 vs 30mm

"I have usually heard it described as the FoV you'd get at 1x..." Yes, that's right; thanks, Scarodactyl. It is still a cumbersome number. So you have a real FOV with a 60x objective, e.g., that in microns will be (FOVmm*1000)/60). Fine; you want to measure a critter's length for a scale bar on the ...
by wporter
Sun Feb 09, 2020 5:39 pm
Forum: Microscopes and optics
Topic: 23.2 vs 30mm
Replies: 38
Views: 7914

Re: 23.2 vs 30mm

Well said, Phil. Nice summary. I, too, would prefer standardization on apparent field. FOV in mm seems somehow extracted from some sort of optical design calculations, with not much users can relate to. It would be nice to have an easy way to determine apparent field of an eyepiece (relative field s...