MicrobeHunter.com Microscopy Forum

That looks like a vintage sextant (telescope) not a microscope!

It is easy for heads to get misaligned without obvious signs or marks. It was probably dropped at some point or maybe even made a little wrong. I was able to get this realigned by taking a slide and inscribing a crosshair on it using a carbide point. There were no adjustments on the oculars, but th...

I have two scopes with binocular heads, one an AO and one a Swift. The Swift has a Siedentopf head where the interpupillary distance is changed by a center pivot like on standard field binoculars. The AO unit is changed in linear fashion on a dovetailed fixture. I have no problems getting a single i...

The ring had me slightly confused because of the way it was shown in the catalog page. See photo. I believe it is shown upside down. But because of the way the ring is made I think that it is used to hand-hold the petri dishes so you can move the dish under the scope for examination. There is a smal...

I ran across some of the original accessories for the M2 along with one I didn't know existed, a fixed base. I picked them up for a reasonable price and with one more purchase, I think I will have all the accessories offered for the M2. Picture of what I purchased:

Some information about the microscope, the objectives and the samples would help to answer your question. Do you use cover slides? How thick they are? How are your samples embedded? Do you use immersion oil? The 100x is most probably an immersion design. The working distance of 40x and 100x objecti...

Its a training scope, the split head allows two people to view the same sample simultaneously. We have a few similar models kicking around our department from the 1960's (as curios, we don't use them any more). Versions with dual binocular heads were also made, and a few manufacturers still make du...

The price and value depend on whether the instrument has been cleaned, adjusted and tested. You can get AO Sixty student microscopes by the dozens, really cheap on Ebay. Most of them are engraved with the institutions name/number somewhere on the unit. I got a couple of them and was able to mix-and...

Here is the same thing at a much reduced price: https://www.ebay.com/itm/384825254892?hash=item59995f63ec:g:kUMAAOSwX5tiUXqL Boy, that second one looks like a sweetheart of a deal, perfect for the curiosity shelf of an enthusiast and priced about right. ........ That one got scooped up in a hurry. ...

Here is the same thing at a much reduced price:

https://www.ebay.com/itm/384825254892?h ... SwX5tiUXqL

I don't know your setup, so don't know if these simple lens formulas will help: 1/Do + 1/Di = 1/f Do=distance of object from lens, Di=distance of image from lens, f = focal length of lens. Si/So = Di/Do Si=size of image, So=size of object With virtual images involved, it will require drawing the len...

At least the seller knew enough not to advertise it as a "binocular" microscope. He could use a little math lesson though!

SPI makes a series of reticles for their handheld comparators that can be put on your stage and are somewhat less expensive than a slide specifically made for a microscope. See the pdf file.

Try one of these lens "suckers" used for camera lens repair. The kit comes with very small suction cups, which are perfect for coverslip holding and positioning:

https://www.ebay.com/itm/175019537986?e ... Swrchg2UYJ


Added photo:

Hi everyone and thank you for all that I have already learned from this forum...first post. ....... Similarly, when using my higher magnification objectives (40x, 100x) it seems I have so many floaters in my eyes that come into focus (clearly moving with my eye and blinking) that objective cleanlin...

Yes. Before I retired I re-coverslipped a couple hundred teaching slides I had made as a student and mounted with Permount. Soak in Xylene if you can. It's a lot faster than the citrus-based solvents. They work, they're just slower. Xylene should loosen the coverslip in a couple of days. Flood the ...

Would you happen to have a 1031 illuminator in your inventory of parts. I would be interested if you do. Thank you.

You might want to try a ham radio supply store. You need a minimum 10 amp supply, and many ham radio supplies are 20 - 30 amps at 12v. Many listed on ebay for less than $100.00 USD.

....... If, as it seems, these diaphragms are added between field lens and condenser, then they cannot be at an illumination focal plane unless extra lenses are also involved. Your statement would indicate that on Kohler systems the diaphragm is between the light source and light source lens (field...

Well Louise, thank you for all your efforts! I didn't mean for anyone to lose sleep over this. :) There is no way for me to get results like you have shown on anything higher than the 10x objective. I have adjusted the height through its entire range, about 5mm, and the condenser diaphragm is only a...

My old AO Sixty doesn't have a field diaphragm so I made a fixed hole diaphragm that slips over the light source and is dead-center to the source (I have a lathe, milling machine, etc. so not a big deal.) The hole in the "diaphragm" is 1.5mm. When looking at it with the 10X objective, the hole edge ...

An older thread, but rather than start one on the same subject, I thought I would ask my questions here. On the posted video, and from other sources, they say to realign the optics every time you change powers (objectives). Being somewhat illiterate in this area (Wil Rogers once said, "Everyone is i...

What anomalies can I expect with this objective in this scope? One beauty of infinity objectives is that tube length mismatch mostly affects magnification factor. With suitable high contrast rectilinear targets, chromatic, spherical and other aberrations may be evident, particularly in camera image...

I was able to get a 20x AO Spencer C80874 objective to fill the gap in my 160/0.17 objectives (10x, now 20x, 40x,100x) and decided to try it in my AO Sixty infinity scope just for kicks. Low and behold I was able to get a perfect image on this scope. It was .014" short for parfocal to which I added ...

Ditto the above. Especially many in the newcomers intro section!

Welcome. What are your particular interests in microscopy? There is much knowledge here and many willing to share it.

BramHuntingNematodes wrote: ↑

Fri Jun 24, 2022 1:40 pm

Mite of some kind. Birds are full of them!

Thought it "might" be!

Cleaning our Bluebird house after the last hatch left and found literally thousands of these bugs all over the outside of the house. I used a small drop of oil to wet a small stick and picked up a couple of these critters and put them on a slide. I then held my camera over the eyepiece and got this ...

Excellent detail as usual! Just curious what instrumentation do you use in your evaluations. I have always been curious, especially in color renditions, as to how comparisons are made. I know in photography we have master color charts, e.g. Tiffen Color Separation Guide and Grey Scale. Are there sim...

With the exception of only a few early infinity corrected apochromats that I know of ( 10X .30, 43X .80 and 80X 1.30) the infinity corrected objectives have the catalogue # engraved on the objective barrel. They are rarely 3 numbers and normally 4 numbers. There were rarely serial #'s on the infini...