MicrobeHunter.com Microscopy Forum

Yes you're right, edge's sharpness is also important for stitching. I am thinking that i can crop the best clear area from the bigger images (from the vignetted) maybe this work but I am not sure without try the camera I think Xline Olympus objective is good for this kind of situation ( but very exp...

Mu purpose is seeing bigger field of view. For example when scanning 15mmx15mm area, I have to take 650 photos (0.75mm x 0.55 mm). If I see bigger area for example (like 0.85mm x 0.75mm) I need less image and as a result less time to scan a tissue

@Hobbyst46 actually it is a scanner, objectives's FN value is 26.5 Tube lens information: Focal length : 180mm Projection area: 26.5mm, Focal Plane Position: 151.3mm 0.74mm obtained from 40x 0.95NA objective. When I checked image diameter through the eyepieces, (Olympus CX23 microscope) diameter is ...

First of all thank you for fast reply

Our equipment consist of ;

40x UPlanApo 0.95NA objective is purchased from Chinese
0.35X C-mount Camera adaptor and 1X Tube lens (both of them are from Olympus)

Actually the image on the tube lens is bigger than 0.75 x 0.55 mm, but camera has limited f.o.v.

Hello, happy new year. Hope you're very well We have a 12MP camera, it's attributes here: Sensor: Sony 1/1.7"(7.40 x 5.55 mm) Pixel size: 1.85 mikrometre 25 FPS (It has 50 fps mode but small field of view) Our optical system consists of 0.35X camera adaptor, 40X 0.95NA objective and 1X tube lens Whe...

Thank you @Scarodactyl I will also consider your shared product. So just to clarify something, How much bigger area can I see with best optical system at 40x ? For example https://www.leicabiosystems.com/digital-pathology/scan/aperio-gt-450/ (please navigate to COMPARE section)in this site there are...

Thank you for your time and help

I will also look at the helicoid adapter or variable extension tube at some later. I will go ahead same setup for now

Have a good day

Please see other images

Hi again, I have played some configuration within our optic system. I just put tube lens reversed and see that magnification decreased and field of view increased but image was not good as before. (I just wondered that how this will affect the situation) Field of view increased about 0.1 mm after tu...

Thank you for your time and your detailed answers.

So I will try with 0.5x tube lens and i will investigate the how 0.5x tube lens affect magnification and field of view

Thank you again

Hi, sorry for the late reply. Yes you're right tube lens is important factor. "How did you determine that the objectives do not magnify 40X in a CX 23?" As I understand from the images taken with my phone camera, same objects has different pixel length. Actually I tested with 20x objectives that fie...

As I understand I should play with tube lens specification right ? Please see the attached photos 40x Chinese Not Olympus objective show biggest area but in microscope and camera system 40x 0.65 Olympus objective show biggest area Yes 40x 0.65 is from CX23 Microscope. When I look at the CX23 Microsc...

Also objectives are added

Yes @apochronaut i agree with you 40X Olympus UPLFLN objective is magnifying more than the 40X Olympus Plan objective. Yes 40x UPlanApo 0.95NA objective is purchased from Chinese and it is not Olympus. I also viewed with microscope(Olympus CX23) and I see that 40x 0.65 is not magnify 40x or others i...

First of all thank you for your answers. Adaptor and Tube Lens purchased from Olympus. We use C-mount 0.35x adaptor Here is the setup and images taken with different objectives Our Camera's sensor info is 1/1.7“(7.40x5.55) Yes 40x 0.65 objective showing wide area but quality is lower than Olympus 40...

Hello, I am making automated microscope system. I am scanning 15mmx15mm tissue with 40x Objective, 0.35x Adaptor and 1x tube lens, and also my camera is 12MP CMOS area scan camera - My first Objective is Olympus Plan 40x 0.65NA and FN Value 20. When imaging the tissue, my field of view is 0.84 mm x ...