Search found 116 matches

by Tom Jones
Mon Nov 25, 2019 5:07 pm
Forum: Beginner's corner
Topic: thinking out loud here - moral thoughts
Replies: 13
Views: 620

Re: thinking out loud here - moral thoughts

Ignoring for the moment the fact that everything we do each day involves the death of a lot of organisms in one way or another... :shock: so many that the number of them in your sample doesn't even reach the level of a rounding error... :D How many organisms are you going to squash to death :oops: w...
by Tom Jones
Sun Nov 24, 2019 4:15 pm
Forum: Microscopes and optics
Topic: There is DIC, then there is DIC
Replies: 10
Views: 687

Re: There is DIC, then there is DIC

ALL transmitted light DIC requires polarized light and two Nomarski prisms. Reflected light DIC requires only one as the light passes through it twice. All of the prisms are expensive and limited as to the number of objectives they will work with, often being designed to work with one specific objec...
by Tom Jones
Sat Nov 23, 2019 10:26 pm
Forum: Camera systems and imaging
Topic: How to attach camera to Olympus BH2?
Replies: 19
Views: 3524

Re: How to attach camera to Olympus BH2?

I use this Fotodiox C-m4/3 adapter with the U-PMTVC for my BHS and SZH: https://www.adorama.com/fdcmft.html?gclsrc=aw.ds&gclid=CjwKCAiAzuPuBRAIEiwAkkmOSLDVCc7HsAdeIxWC6Q-6pdBsB9dC3WFL3sfe-VSt2j1RVSrDn_iH3xoCLt4QAvD_BwE It screws down flush against the PMTVC adapter, but works fine with the GH4. The ...
by Tom Jones
Thu Nov 21, 2019 5:25 pm
Forum: Camera systems and imaging
Topic: How to attach camera to Olympus BH2?
Replies: 19
Views: 3524

Re: How to attach camera to Olympus BH2?

Some of the available micro 4/3 C-mount adapters don't like the flat top on the U-PMTVC and won't screw on completely before they bottom out. Mine just barely makes it.

That could make the adapter-camera sensor distance too long even with the adapter fully screwed together.
by Tom Jones
Thu Nov 21, 2019 3:34 pm
Forum: Camera systems and imaging
Topic: Help attaching full frame DSLR to Olympus BX40
Replies: 19
Views: 511

Re: Help attaching full frame DSLR to Olympus BX40

John, You'll need a PE 2.5x projection lens (for 35 mm full-frame cameras), a U-SPT photo tube (or U-DPT if you want to mount two cameras) to mount on top of the trinoc head, and a camera adapter such as the Diagnostic Instruments PA1-10A with a Canon T-ring mounted on top of that. The DA instrument...
by Tom Jones
Thu Nov 21, 2019 8:17 am
Forum: Camera systems and imaging
Topic: How to attach camera to Olympus BH2?
Replies: 19
Views: 3524

Re: How to attach camera to Olympus BH2?

Are you saying you cannot get the camera by itself in focus, or cannot get the camera focus and eyepiece focus to match?

If the camera image won't come into focus, something is wrong.
by Tom Jones
Sun Nov 10, 2019 9:48 pm
Forum: Camera systems and imaging
Topic: How to attach camera to Olympus BH2?
Replies: 19
Views: 3524

Re: How to attach camera to Olympus BH2?

It can be. The U-PMTVC has screw threads that will allow you to adjust it's length a bit to make it parfocal. Adjust focus in the right eyepiece, match it with the left, then adjust the U-PMTVC (screw it in or out) until the camera focus matches what you see in the eyepieces. I use a -H focusing eye...
by Tom Jones
Fri Nov 08, 2019 3:31 am
Forum: Specimens, samples and slides
Topic: can i wash and reuse bacterial heat set slides?
Replies: 5
Views: 581

Re: can i wash and reuse bacterial heat set slides?

Of course you can reuse the slides. We tossed them into Hazardous Waste in the hospital lab, but that was because of the glass, not the organisms. In a hospital it is rarely cost effective to wash things like slides. Labor costs too much, particularly when you're doing 50-100 gram stains a day. Scho...
by Tom Jones
Sat Oct 19, 2019 4:31 pm
Forum: Specimens, samples and slides
Topic: Disposing of stains from home microscopy lab
Replies: 8
Views: 721

Re: Disposing of stains from home microscopy lab

Mike, Go to the manufacturer's (or any manufacturer of the stain) web site and look for the Safety Data Sheets/Material Safety Data Sheets. They contain all of the relevant safety and disposal information. See for example: https://www.tedpella.com/SDS_html/19451_sds.pdf The stains mentioned are in u...
by Tom Jones
Sun Sep 22, 2019 4:00 pm
Forum: For forum members who want to buy and sell equipment
Topic: NO LONGER WANTED Olympus intermediate polariser
Replies: 31
Views: 2961

Re: WANTED Olympus intermediate polariser

The BH-2 simple pol adapter won't fit the BX. The dovetail is too small. It will actually go on, but isn't centered. The bigger problem is that the BX head dovetail is too big to fit the BH-2 adapter. I just checked. You would need to use a BH-2 head with it, and figure out how to center it... You h...
by Tom Jones
Fri Aug 30, 2019 3:53 am
Forum: Beginner's corner
Topic: Compound scope for home education
Replies: 18
Views: 1852

Re: Compound scope for home education

opticnewb,

Where are you located? Maybe someone is close enough to provide a bit of assistance.

Tom
by Tom Jones
Sat Aug 10, 2019 2:57 pm
Forum: My microscope
Topic: My new Olympus BHS BH2
Replies: 39
Views: 4704

Re: My new Olympus BHS BH2

PeteM, No, I don't know off hand. I've never tried the BH system at all. The BX and BH2 condensers will interchange in the mounts, and I've been told the removable prisms are the same size, but whether they will interchange optically or not I don't know. Given the variability in the BX series - oil ...
by Tom Jones
Sat Aug 10, 2019 4:10 am
Forum: My microscope
Topic: My new Olympus BHS BH2
Replies: 39
Views: 4704

Re: My new Olympus BHS BH2

Hi Dan,

Here are the photos of the opened BH-2 DIC condenser.
Pretty simple. If you use a small phillips instead of a JIS, just very very careful as it won't fit quite as well as it should.

Tom
by Tom Jones
Sat Aug 10, 2019 1:53 am
Forum: My microscope
Topic: My new Olympus BHS BH2
Replies: 39
Views: 4704

Re: My new Olympus BHS BH2

Hi Dan, A little experimenting this afternoon to answer your questions. This presumes you have the condenser with the fixed DIC prisms and phase rings, labeled "NIC/Phase Contrast" I installed the mag changer above the DIC intermediate tube, directly under the head, and it works fine, at least visua...
by Tom Jones
Fri Aug 09, 2019 4:16 pm
Forum: Microscopes and optics
Topic: Olympus BX and BH stage holders
Replies: 2
Views: 802

Re: Olympus BX and BH stage holders

Olympus pol stages are centerable. They also offer centerable objective turrets for the BHT/BHS/BX-50, etc. scopes with removable turrets. Not the BHTU or BX-40 unless you have the rare, removable turret adapter, for the BX-40. A pol scope where you couldn't center specimen rotation with the optical...
by Tom Jones
Thu Aug 08, 2019 4:00 pm
Forum: My microscope
Topic: My new Olympus BHS BH2
Replies: 39
Views: 4704

Re: My new Olympus BHS BH2

Hi Dan, Give me a couple of days to check on the Mag changer/DIC compatibility and your other DIC questions. I'm off to Hollywood this morning to do some video photomicrography training with a film maker friend in her studio, so I won't be able to play with my BHS until at least tomorrow or Saturday...
by Tom Jones
Thu Aug 08, 2019 4:48 am
Forum: My microscope
Topic: My new Olympus BHS BH2
Replies: 39
Views: 4704

Re: My new Olympus BHS BH2

First, I'd suggest you peruse this site: http://www.krebsmicro.com/ Charlie Krebs is the BHS-DIC Photomicrography Guru. Pretty much all of your questions are answered there. The projection eyepieces are designed to stay away from the outer edges of the field where image quality can be a bit less. Th...
by Tom Jones
Thu Jul 25, 2019 2:24 pm
Forum: Beginner's corner
Topic: Objectives
Replies: 8
Views: 1763

Re: Objectives

I'd MUCH rather have a good 20x than a good 100x. Amateurs rarely need that much magnification. Good 60x objectives are nice, but more rare so more costly for good ones, and generally not necessary. I removed the 100x objectives from my seven BH-2 outreach scopes several years ago. They all have nic...
by Tom Jones
Tue Jul 16, 2019 2:51 pm
Forum: Microscopes and optics
Topic: How to make a lambda wave plate?
Replies: 16
Views: 1397

Re: How to make a lambda wave plate?

Look at the 50mm x x50mm. Full, 1/2 and 1/4 wave. $18 each for the full wave, $12 each for the 1/2 and 1/4. Click on "Specifications and Documents" below each description to get the full data, including thickness. Put them below the condenser as they are not optically clear enough to go between the ...
by Tom Jones
Thu Jul 04, 2019 11:34 pm
Forum: Pictures and Videos
Topic: Mayfly Nymph
Replies: 14
Views: 1981

Re: Mayfly Nymph

Thanks Roldorf and MichaelG! About a 30 second clip from that ended up on Discovery Canada's science show Daily Planet, in an episode of Ziyology, hosted by Ziya Tong and Dan Riskin. Just basking in all the glory here. :roll:

Tom
by Tom Jones
Thu Jul 04, 2019 1:22 am
Forum: Beginner's corner
Topic: Do you think that this is a fair comparation between this two microscope (Bx46 and Axio Lab A1)?
Replies: 14
Views: 1309

Re: Do you think that this is a fair comparation between this two microscope (Bx46 and Axio Lab A1)?

Call the Olympus and Zeiss reps and have them bring out a demo of the scopes you are considering, and leave it/them for a week or two so you and your techs can see how you like it. Ask them what they would recommend, too. Get a bid on what you want. It will probably be for less than list. I don't kn...
by Tom Jones
Sat May 04, 2019 3:09 am
Forum: Identification help
Topic: Unknown blood cells in blood sample
Replies: 6
Views: 2186

Re: Unknown blood cells in blood sample

:roll: You should stop doing tests on yourself if you don't know what you're doing. :?

Perhaps a perusal of this might help: https://onlinelibrary.wiley.com/doi/boo ... 1444398595 8-)

On the other hand, I've heard Gwyneth Paltrow's Goop might be good for this... :twisted:
by Tom Jones
Sun Apr 14, 2019 3:47 pm
Forum: Pictures and Videos
Topic: Tips on Buying a Microscope and suggestions for What to look at
Replies: 16
Views: 4331

Re: Tips on Buying a Microscope and suggestions for What to look at

Very comprehensive and very nicely done! Not to be too picky, but just below your image of two older microscopes, you indicate the Unitron microscope is on the left, while it is actually shown on the right. ;)
by Tom Jones
Tue Apr 02, 2019 3:39 pm
Forum: Camera systems and imaging
Topic: How to attach camera to Olympus BH2?
Replies: 19
Views: 3524

Re: How to attach camera to Olympus BH2?

I use that very setup to project, essentially direct project as the NFK 3.3x and 0.3x lens in the U-PMTVC essentially cancel magnifications, onto the sensor of my Panasonic GH4 for 4K video. Since the GH4 is mirrorless, a C-mount adapter fits fine, and I get the necessary final corrections in the NF...
by Tom Jones
Fri Mar 08, 2019 3:52 am
Forum: Microscopes and optics
Topic: 100x NCG oil: what is it used for?
Replies: 5
Views: 1139

Re: 100x NCG oil: what is it used for?

Almost all clinical labs use standard 100x 0.17 oil immersion objectives, with oil and without cover slips, for blood smears, body fluid smears, or gram stains for that matter. Lower power objectives are used with a thin film of oil substituting for the cover slip there as well. Cover slips are cost...
by Tom Jones
Fri Feb 08, 2019 1:36 am
Forum: Pictures and Videos
Topic: Aeolosoma sp.
Replies: 11
Views: 1832

Re: Aeolosoma sp.

Nice. It's even budding!
by Tom Jones
Sat Feb 02, 2019 12:36 am
Forum: Beginner's corner
Topic: Picture magnification question?
Replies: 8
Views: 2459

Re: Picture magnification question?

One other thing to consider is to be sure the picture/video format you want to use is the same one you use to calibrate. Most cameras will use different pixel dimensions on the sensor for each available resolution and/or aspect ratio. That can rather dramatically change the number of pixels for a gi...
by Tom Jones
Mon Jan 28, 2019 5:05 am
Forum: Beginner's corner
Topic: Eye floaters
Replies: 14
Views: 4482

Re: Eye floaters

GOLGO13, Ignoring your floaters is something that is pretty much universally recommended. It's generally good advice for most people. However, if they are really debilitating, you actually have a couple of choices to get rid of them. It will be a bit of a slog to convince the average ophthalmologist...
by Tom Jones
Wed Jan 09, 2019 5:17 pm
Forum: Off Topic
Topic: This FREE 6-week course in how to design experiments in biological research..
Replies: 2
Views: 3230

Re: This FREE 6-week course in how to design experiments in biological research..

You're right Billt. That does look interesting. Thanks! For those not aware, in addition to classes like this one, iBiology has many video lectures on various aspects of biology research. They have also a very nice, and very complete, lecture series on microscopy techniques: https://www.ibiology.org...