MicrobeHunter.com Microscopy Forum

Aeolosoma hemprichi is very easy to keep in culture too. I kept some going for three years in a deep petri dish. Just feed them a little piece of spinach leaf once a week or so, and change the water when there gets to be too much debris left over. I've seen as many as four of them hooked together no...

Which Sony? Find out what part of the sensor is being used for the video resolution you intend to shoot. If it shoots video using the full frame at that resolution, your good with the NFK 2.5x. If it's smaller than full frame, you'll either need to accept the crop factor or come up with another solu...

Color provides contrast among other things. Without color, you will need to find some other way to increase contrast enough to tell one thing from the other. It also provides more specific clues as to what you are looking at by way of differential staining. Think of the difficulty in distinguishing ...

I'm curious to know in what hematology applications field width is prized over all else, including color?

No, actually. I won't modify my photography systems to take pictures for you. And I have no inclination to check or comment on your math. I've explained what I use and why. Asking me to do testing for you so you can figure out why your math is confusing or misleading you is unreasonable, and not som...

The + inside a circle should mean there is a crosshair reticle in place. These are mostly used on polarizing microscopes and generally cost somewhat more than an eyepiece without the reticle as they are relatively rare. They are also handy if you are trying to collimate the eyepieces in a binocular ...

The bottom line is that you need an image circle at the sensor that is large enough to enclose the entire area of the sensor that is used to capture the image. If it's smaller, you get vignetting. You really have very few reasonable choices as to how to get there. You are limited to the available pr...

ScienceMatters - I just sent you a private message.

Tom

I only use the GH4 for video on the microscopes, so I have no experience with it's electronic shutter in the photography mode.

Tom

A mag changer can help with photographic framing. You can actually do the same thing with different projection eyepiece magnifications too. I originally got one to help with framing specimens for outreach demonstrations, so the kids didn't have to search so much for what I was trying to show them. A...

I think using a calibration slide is the only way to get accurate dimensional information for scale bars. Basically, Photograph the calibration slide with the objectives you intend to use (or all of them to be complete) with the same camera and projection setup you will use with the specimens. If yo...

I recommended the GH4 because I've had one since they were new, and I know it works well in this application. It's also pretty cheap (used) right now. Origianally it was recommended to me by a microscopy/photography professor friend. The GH5 is newer, same sensor and mount, but probably ~$1500. I kn...

Easily done. Panasonic GH4 (~$500-600 used), M4/3 to C-mount adapter(~$10, Fotodiox, Fotasy, etc.), NFK 3.3x (~$50-75), U-PMTVC 0.3x C-mount to microscope adapter (~$100-125). The combination of the 3.3x and 0.3x is close to 1x, so you have essentially direct projection, with the color correction ne...

Those are designed for long-term storage of pathology slides, and for that they work very well. They're very compact for the number of slides they contain. Sliding stops or even foam blocks, are used to keep the slides vertical. Small cards can delineate time, or specimen number ranges. Modern mount...

I use these Diagnostic Instruments PA1-10A adapters. They work really well on either BH2 or BX series microscopes in place of the Photomicro Adapter L: https://www.ebay.com/itm/Nikon-F-Mount-to-Olympus-BH2-BX-Microscope-DSLR-Camera-Adapter-PA1-10A/223686036249?hash=item3414ba0b19:g:eYAAAOSwTvNdkLqF ...

It might be a bit excessive, and it's certainly thorough, but there's always this to help you make up your mind: https://www.amazon.com/Conns-Biological ... 1859960995

The BX-50 is an excellent microscope. I don't know who mentioned the sunburned eyes, but they don't know what they're talking about. These microscopes are designed for research and clinical use where they are used for hours on end. I don't remember any of my colleagues in clinical labs complaining a...

Actually, unless you are trying to demonstrate how a deaf person would view your slideshow 8-) , some kind of music or background sound is better. A slide show without sound or narration is like handing friends a series of printed still image without comment. :twisted: You probably wouldn't do it. E...

Dilute the drop of blood immediately with saline. Put the saline drop on the slide first, then a small drop of blood and mix.

Per your Wiki link: Psychophysics quantitatively investigates the relationship between physical stimuli and the sensations and perceptions they produce. Psychophysics has been described as "the scientific study of the relation between stimulus and sensation"[1] or, more completely, as "the analysis ...

Anton, I personally think that particular microscope is a good deal. Synchrostream has been around a long time and sells quite a few microscopes and parts. They offer a 30 day return, and free shipping. Properly packaged shipping's about $100 by itself. It's not the cheapest, but it's well within yo...

I have a spare OM adapter I'll sell, but no spare NFK 2.5x photoeyepiece.
$125 plus insured shipping. PM me if you're interested.

Tom

While lots of sellers are clueless, there are good ones too. Synchrostream has been around for a long time and is quite aware as to what they are selling. Not cheap, but I've bought from them before and been satisfied. Notice too the 30 day return period. That's not something a dicey seller will usu...

Is the microscopic analysis you're planning to do in addition to, or in lieu of normal oil (spectrographic, etc.) analysis?? Among others... https://www.blackstone-labs.com/tests/standard-analysis/ I have an oil analysis done, cut the oil filter and inspect the element each time I change oil on my a...

Wow Carl. Nicely done!

Floaters are inside your eye. There is nothing you can put in your eyes that will get rid of them. The best option is to learn to ignore them. If they're bad, the next best option is laser vitriolysis, where a YAG laser is used by an ophthalmologist to disrupt them as much as possible. There are fou...

Just out of curiosity, what do you consider to be a reasonable price for the NFK 2.5x and NFK 1.67X?

Maryfox, As you have found out, basic microscopy with low magnifications and relatively low expectations is pretty easy. Once you start up the slope wanting more, it gets a lot more complicated. I've posted this once before several years ago http://www.microbehunter.com/microscopy-forum/viewtopic.ph...

Actually, using that microscope, I think your best bet is to use your cell phone. It's much easier than it sounds. They can certainly shoot 1080p and probably shoot 4k video if you would like. They can also be wired to an external monitor as well. You'll get to see on your screen everything your eye...

PeteM is right. My vote is for the Olympus BX series. I was a clinical lab supervisor in the second busiest trauma center in California for a long time. In 1999, when we built a new hospital, we switched from AO's and Olympus BH-2s, to Olympus BX-40's in the clinical lab (micro, UA, and hematology)....