MicrobeHunter.com Microscopy Forum

Ignoring for the moment the fact that everything we do each day involves the death of a lot of organisms in one way or another... :shock: so many that the number of them in your sample doesn't even reach the level of a rounding error... :D How many organisms are you going to squash to death :oops: w...

ALL transmitted light DIC requires polarized light and two Nomarski prisms. Reflected light DIC requires only one as the light passes through it twice. All of the prisms are expensive and limited as to the number of objectives they will work with, often being designed to work with one specific objec...

I use this Fotodiox C-m4/3 adapter with the U-PMTVC for my BHS and SZH: https://www.adorama.com/fdcmft.html?gclsrc=aw.ds&gclid=CjwKCAiAzuPuBRAIEiwAkkmOSLDVCc7HsAdeIxWC6Q-6pdBsB9dC3WFL3sfe-VSt2j1RVSrDn_iH3xoCLt4QAvD_BwE It screws down flush against the PMTVC adapter, but works fine with the GH4. The ...

Some of the available micro 4/3 C-mount adapters don't like the flat top on the U-PMTVC and won't screw on completely before they bottom out. Mine just barely makes it.

That could make the adapter-camera sensor distance too long even with the adapter fully screwed together.

John, You'll need a PE 2.5x projection lens (for 35 mm full-frame cameras), a U-SPT photo tube (or U-DPT if you want to mount two cameras) to mount on top of the trinoc head, and a camera adapter such as the Diagnostic Instruments PA1-10A with a Canon T-ring mounted on top of that. The DA instrument...

Are you saying you cannot get the camera by itself in focus, or cannot get the camera focus and eyepiece focus to match?

If the camera image won't come into focus, something is wrong.

It can be. The U-PMTVC has screw threads that will allow you to adjust it's length a bit to make it parfocal. Adjust focus in the right eyepiece, match it with the left, then adjust the U-PMTVC (screw it in or out) until the camera focus matches what you see in the eyepieces. I use a -H focusing eye...

Of course you can reuse the slides. We tossed them into Hazardous Waste in the hospital lab, but that was because of the glass, not the organisms. In a hospital it is rarely cost effective to wash things like slides. Labor costs too much, particularly when you're doing 50-100 gram stains a day. Scho...

Mike, Go to the manufacturer's (or any manufacturer of the stain) web site and look for the Safety Data Sheets/Material Safety Data Sheets. They contain all of the relevant safety and disposal information. See for example: https://www.tedpella.com/SDS_html/19451_sds.pdf The stains mentioned are in u...

The BH-2 simple pol adapter won't fit the BX. The dovetail is too small. It will actually go on, but isn't centered. The bigger problem is that the BX head dovetail is too big to fit the BH-2 adapter. I just checked. You would need to use a BH-2 head with it, and figure out how to center it... You h...

opticnewb,

Where are you located? Maybe someone is close enough to provide a bit of assistance.

Tom

PeteM, No, I don't know off hand. I've never tried the BH system at all. The BX and BH2 condensers will interchange in the mounts, and I've been told the removable prisms are the same size, but whether they will interchange optically or not I don't know. Given the variability in the BX series - oil ...

Hi Dan,

Here are the photos of the opened BH-2 DIC condenser.
Pretty simple. If you use a small phillips instead of a JIS, just very very careful as it won't fit quite as well as it should.

Tom

Hi Dan, A little experimenting this afternoon to answer your questions. This presumes you have the condenser with the fixed DIC prisms and phase rings, labeled "NIC/Phase Contrast" I installed the mag changer above the DIC intermediate tube, directly under the head, and it works fine, at least visua...

Olympus pol stages are centerable. They also offer centerable objective turrets for the BHT/BHS/BX-50, etc. scopes with removable turrets. Not the BHTU or BX-40 unless you have the rare, removable turret adapter, for the BX-40. A pol scope where you couldn't center specimen rotation with the optical...

Hi Dan, Give me a couple of days to check on the Mag changer/DIC compatibility and your other DIC questions. I'm off to Hollywood this morning to do some video photomicrography training with a film maker friend in her studio, so I won't be able to play with my BHS until at least tomorrow or Saturday...

First, I'd suggest you peruse this site: http://www.krebsmicro.com/ Charlie Krebs is the BHS-DIC Photomicrography Guru. Pretty much all of your questions are answered there. The projection eyepieces are designed to stay away from the outer edges of the field where image quality can be a bit less. Th...

I'd MUCH rather have a good 20x than a good 100x. Amateurs rarely need that much magnification. Good 60x objectives are nice, but more rare so more costly for good ones, and generally not necessary. I removed the 100x objectives from my seven BH-2 outreach scopes several years ago. They all have nic...

Look at the 50mm x x50mm. Full, 1/2 and 1/4 wave. $18 each for the full wave, $12 each for the 1/2 and 1/4. Click on "Specifications and Documents" below each description to get the full data, including thickness. Put them below the condenser as they are not optically clear enough to go between the ...

Thanks Roldorf and MichaelG! About a 30 second clip from that ended up on Discovery Canada's science show Daily Planet, in an episode of Ziyology, hosted by Ziya Tong and Dan Riskin. Just basking in all the glory here.

Tom

Call the Olympus and Zeiss reps and have them bring out a demo of the scopes you are considering, and leave it/them for a week or two so you and your techs can see how you like it. Ask them what they would recommend, too. Get a bid on what you want. It will probably be for less than list. I don't kn...

Conn's: https://www.amazon.com/Conns-Biological ... B0006COHY2

You should stop doing tests on yourself if you don't know what you're doing.

Perhaps a perusal of this might help: https://onlinelibrary.wiley.com/doi/boo ... 1444398595

On the other hand, I've heard Gwyneth Paltrow's Goop might be good for this...

Very comprehensive and very nicely done! Not to be too picky, but just below your image of two older microscopes, you indicate the Unitron microscope is on the left, while it is actually shown on the right.

I use that very setup to project, essentially direct project as the NFK 3.3x and 0.3x lens in the U-PMTVC essentially cancel magnifications, onto the sensor of my Panasonic GH4 for 4K video. Since the GH4 is mirrorless, a C-mount adapter fits fine, and I get the necessary final corrections in the NF...

Almost all clinical labs use standard 100x 0.17 oil immersion objectives, with oil and without cover slips, for blood smears, body fluid smears, or gram stains for that matter. Lower power objectives are used with a thin film of oil substituting for the cover slip there as well. Cover slips are cost...

Nice. It's even budding!

One other thing to consider is to be sure the picture/video format you want to use is the same one you use to calibrate. Most cameras will use different pixel dimensions on the sensor for each available resolution and/or aspect ratio. That can rather dramatically change the number of pixels for a gi...

GOLGO13, Ignoring your floaters is something that is pretty much universally recommended. It's generally good advice for most people. However, if they are really debilitating, you actually have a couple of choices to get rid of them. It will be a bit of a slog to convince the average ophthalmologist...

You're right Billt. That does look interesting. Thanks! For those not aware, in addition to classes like this one, iBiology has many video lectures on various aspects of biology research. They have also a very nice, and very complete, lecture series on microscopy techniques: https://www.ibiology.org...