MicrobeHunter.com Microscopy Forum

Antartica, From a practical point of view, having nicer objectives serves no purpose unless, and until, you need them. If you are " not experienced enough to see differences ", buy a decent microscope and learn how to use it. When you are no longer limited by your experience, and can tell the differ...

Filters can just drop in on top of the light port. No extra holder is necessary. The little filter holder that goes in the front of the lamp house holds the same size filters. I prefer dropping them in on the light port on the occasions where I use one not included in a condenser. If you feel like s...

Could anyone comment on this listing: https://www.ebay.com/itm/OLYMPUS-BH2-TR ... Sw7t9eVZoD With four DPlans and a trinoc head, and free shipping, it looks like a pretty good price to me, and I have a BHS. Importantly, the seller says it's tested, works fine, and backs it up by accepting returns u...

To insert a little reality here, while you might, eventually find something new, it is extremely unlikely . :? In order to find something new, you must generally be able to identify all of the currently known organisms of the type you are studying. Why? Unless you can identify potentially each piece...

Guide to Microlife, Rainis and Russel: https://www.amazon.com/Guide-Microlife-Science-Life-Environmental/dp/0531112667/ref=sr_1_1?keywords=guide+to+microlife+rainis&qid=1583805774&sr=8-1 Free-Living Freshwater Protozoa, a Color Guide, Patterson: https://www.amazon.com/Free-Living-Freshwater-Protozoa...

I've always preferred a 50:50 mixture of high and low viscosity for normal work in clinical labs, and that's what I use at home.

Buying both to mix, while a bit more expensive, has the side benefit of allowing you to try all three and stay with what you find most convenient for the work you do.

No. The HDMI output while recording can be in 4K. If you do not have a 4K monitor, it will down sample the signal to full HD, and tell you on screen it is doing so. For external (Ninja, etc.) recording you can get 4K with 10 bit 4:2:2 color as well. You can download the regular and extended manuals ...

So do the cameras recommended here stay on, with HDMI live view, plugged in (ac) indefinitely? The Panasonic GH4 stays on for me, with HDMI out to a monitor, recording continuously in 4K (which is about as hard on battery life as you can get), for between 1 hr 10 min and 1 hr 20 min on a 64GB card ...

Yes. That's the camera.

Tom

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Tom

I've been looking for more or less the same information for some time. I want to assess prism compatibility, both within a manufacturer's system, and between manufacturers, without actually having to do direct, and therefore very expensive, comparisons. For example, will a Zeiss prism system work we...

Tom, What you need is a Diagnostic Instruments PA1-10A adapter. It's a direct replacement for the Olympus Photomicro Adapter L, but instead of the Olympus camera mount has a T-ring thread so you can mount any camera you can get a T-ring adapter for. I've used them on both the BH series (BHS), and th...

Tom, Just when you think you have things figured out. :o Thanks for adding the last photos. I hadn't seen the differences in the intermediate tube either. Mine is the BH2 version. Pretty subtle differences. I hope the prisms are the same. Sometimes it's handy to go through a dealer! Pricey, but you ...

Hi Tom, The Stephanoceros video was just normal DIC, no tricks of any kind. I was actually just checking a fresh plankton net collection before I went to a Science Night demo at a local high school when I found it. All the setup and shooting was done in about 30 minutes as I had to leave for the dem...

Damn. I'm sorry. I didn't look at the last picture apparently, as it was mostly a response to the intermediate tube price. I paid about $850 for my intermediate tube. I've never seen that type condenser before. Apparently I got lucky when I bought mine! Since that one doesn't have a dovetail on the ...

Tom, A word to the wise... eat Ramen for a while :? , sell your kids :shock: , just don't sell the DCD, or the DIC set for that matter. Dedicated dark field condensers are better than simple stops, and you're going to like dark field. It's worth learning to swap condensers quickly and efficiently to...

Tom, The Dealcorner intermediate tube is the BH2-NA. It's also too expensive, so he should get to keep it. :o This is why I think it's too expensive: https://www.ebay.com/itm/Olympus-NIC-Phase-Condenser-set-for-DIC-Microscope-setup-in-good-working-order/232936455851?hash=item363c183eab:g:ZL0AAOSwPCZ...

PeteM's right about the DPlans. I used them in hematology and microbiology in a trauma center lab for a long time. They're nice lenses and relatively inexpensive. Get the WHK eyepieces. Their higher eye point is easier to use over a long period of time.

Tom

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Ignoring for the moment the fact that everything we do each day involves the death of a lot of organisms in one way or another... :shock: so many that the number of them in your sample doesn't even reach the level of a rounding error... :D How many organisms are you going to squash to death :oops: w...

ALL transmitted light DIC requires polarized light and two Nomarski prisms. Reflected light DIC requires only one as the light passes through it twice. All of the prisms are expensive and limited as to the number of objectives they will work with, often being designed to work with one specific objec...

I use this Fotodiox C-m4/3 adapter with the U-PMTVC for my BHS and SZH: https://www.adorama.com/fdcmft.html?gclsrc=aw.ds&gclid=CjwKCAiAzuPuBRAIEiwAkkmOSLDVCc7HsAdeIxWC6Q-6pdBsB9dC3WFL3sfe-VSt2j1RVSrDn_iH3xoCLt4QAvD_BwE It screws down flush against the PMTVC adapter, but works fine with the GH4. The ...

Some of the available micro 4/3 C-mount adapters don't like the flat top on the U-PMTVC and won't screw on completely before they bottom out. Mine just barely makes it.

That could make the adapter-camera sensor distance too long even with the adapter fully screwed together.

John, You'll need a PE 2.5x projection lens (for 35 mm full-frame cameras), a U-SPT photo tube (or U-DPT if you want to mount two cameras) to mount on top of the trinoc head, and a camera adapter such as the Diagnostic Instruments PA1-10A with a Canon T-ring mounted on top of that. The DA instrument...

Are you saying you cannot get the camera by itself in focus, or cannot get the camera focus and eyepiece focus to match?

If the camera image won't come into focus, something is wrong.

It can be. The U-PMTVC has screw threads that will allow you to adjust it's length a bit to make it parfocal. Adjust focus in the right eyepiece, match it with the left, then adjust the U-PMTVC (screw it in or out) until the camera focus matches what you see in the eyepieces. I use a -H focusing eye...

Of course you can reuse the slides. We tossed them into Hazardous Waste in the hospital lab, but that was because of the glass, not the organisms. In a hospital it is rarely cost effective to wash things like slides. Labor costs too much, particularly when you're doing 50-100 gram stains a day. Scho...

Mike, Go to the manufacturer's (or any manufacturer of the stain) web site and look for the Safety Data Sheets/Material Safety Data Sheets. They contain all of the relevant safety and disposal information. See for example: https://www.tedpella.com/SDS_html/19451_sds.pdf The stains mentioned are in u...

The BH-2 simple pol adapter won't fit the BX. The dovetail is too small. It will actually go on, but isn't centered. The bigger problem is that the BX head dovetail is too big to fit the BH-2 adapter. I just checked. You would need to use a BH-2 head with it, and figure out how to center it... You h...

opticnewb,

Where are you located? Maybe someone is close enough to provide a bit of assistance.

Tom