MicrobeHunter.com Microscopy Forum

This document will provide more details for you:

https://irp.cdn-website.com/240a28f1/fi ... ns-web.pdf

See especially p. 7-8

Also - how do you fix your smears before staining?

Your pH is most likely too low. Increasing alkalinity will give a more intense colour uptake. Take a look at this brief overview: https://www.merckmillipore.com/NO/en/ivd-oem-materials-and-reagents/learning-center/giemsa-solution/r2ab.qB.aBwAAAFOqm81lSAJ,nav#:~:text=back%20to%20top-,Giemsa%20Stainin...

Right you are, polymerase, the dignity and adherence to 'scientific method of seeking reality outside ourselves'...is the way to go deeper into experiencing objective reality. My 'caution' is my sense that we are quite approaching a paradigm shift...and troubles arise when we educated westerners ca...

So please, polymerase gently have caution with the concept that; 'data will be a lantern to understanding our reality'. data sets come and go..gently understand we are further refining And this, my friend, is the beauty of science. Data sets come and go, and they are all we have. Any data set that ...

Думаю, не стоит ожидать слишком большого количества ответов, если пишите на русском...

Merry Christmas, Joe Henry, and group. Encouragement is the 'coin of the realm' in this microscopy forum ( again, thank you Oliver Kim, for setting this community up and thriving). Remember simple attention to your wet-mount slides..the sandwich of: your microscope slide+ the amount of sample fluid...

Thanks for the replies, I have to admit it was overwhelming however .. much of this stuff is waaaaay too advanced for me :shock: ... not being ungrateful mind you, thanks and merry christmas! Then throw yourself into it, rip your hair out when things don’t work the way you expect, and come back wit...

Wow! Quite expensive with the higher magnifications. Is the import tax because you bought it from somewhere like Japan? Any other fees with buying from places like that? I see most if not all ebay APO's are being sold out of japan and the description always mentions customs fees I haven't researche...

Just for elimination: Did you try adjust interpupillary distance in the viewing head? If improperly adjusted, this can cause both headaches and double vision when viewing. In most heads, distance between the eyepieces can be adjusted. If you haven’t tried it, give it a go. Set the eyepieces to maxim...

Not sure how we all ended up here : this is the beginners corner and a beginners question. Naturally: with proper technique and practice you can use your higher Mag. and NA objectives: don’t see above discussion is helpful to a beginner. This is a very important point! While I don’t disagree with a...

" not in great detail " and I said under 10 microns . You must have been using a 20X .45 objective a lot . Have a look at any protozoology text. Richerd Kudo's for example. All objectives were used and I doubt if any of them was a 20X .45 achromat because that is what most amateurs will be reaching...

What about the myriads of critters under 10 microns not to mention their structures or even the structures of larger critters? A good question. However, viewing such creatures in a live specimen puts a heavy demand on slide preparation. Again, knowing what you intend to study is crucial for selecti...

What about the myriads of critters under 10 microns not to mention their structures or even the structures of larger critters? A good question. However, viewing such creatures in a live specimen puts a heavy demand on slide preparation. Again, knowing what you intend to study is crucial for selecti...

Very often, inexperienced microscopists tend to think that it is all about the high power objectives and high magnification. When starting histo, our professor started by stating that “you”ll be surprised how little time is spent on high power objectives.” While this is (mostly) true for histology s...

I use a Bertrand lens. And if necessary, visual correctiion while working on specimens. Of note, make sure to check that the phase annulus in your condenser has clicked correctly into place before recentering. I made that mistake alot when starting phase contrast. When all it takes is a slight push ...

4x $243 10x $235 20x $297 40x w. corr. collar $372 100x w iris $420 Total of $1.567 + shipping and 25% import tax. These are expensive, and the high magnification objectives are not easy to use. Depth of focus is very shallow, and working distance is extremely small. For wet mounts, I personally wou...

Welcome, both of you!

Do not be afraid to ask questions!

Have you tried the Zeiss archives? I am the owner of no. 48775, which i dated to 1909 by the help of the scanned production books. https://www.zeiss.com/corporate/en/about-zeiss/past/history/archives.html#accordionItem-880734397 If you can’t find it there, try sending an inquiry: https://www.zeiss.c...

Apart from the question of how much money spent, I think the most relevant question is what it is going to be used for. A stereo microscope is probably more instantly rewarding, but is unsuitable for smaller, more transparent objects. What is its intended use? Do you wish to study insects, seeds, su...

I just think you like discipline more than me--maybe. It’s not about liking discipline. More of realizing the benefit of a disciplined and systematic approach. I too, have dreams. Wild and spectacular dreams. But in order to put your dreams under scrutiny, one must have the required tools for doing...

Thanks, Don! Actually, I do agree with you! Dreaming and speculations are absolutely necessary. We just need to distill those dreams into something we can examine. As I stated earlier - deviant thinking pushes science forward. But we still need to be meticulous and methodical. We are not so far apar...

Are you being a complete reductionist? I like to add a little bit of intellectual spice to the broth. Would you care to elaborate? I think we need valid data to conclude, that is all. If you wish to set science aside, and delve into the metaphysical speculation, that is completely ok. But I am afra...

I agree. When you sleep you are only unconscious to someone conscious who observes you. Then there is a block to prevent you from recalling what happened when you were unconscious. People who study dreams find that if a sleeper is aroused suddenly during sleep (especially during the REM portion of ...

Are you sure that unconsciousness is not experienced while unconscious? While it would seem to be the case, you cannot prove it to be the case, see also 'Popper's falsification'. From a neurologic perspective there is no indication that any experience of one’s own consciousness is present during un...

When unconscious you experience unconsciousness. But when waking you just find it hard to recall what it was like, Are you sure? Consciousness is what you can sense. While unconscious, you are essentially turned off, and sensory capability is voided. Therefore, you do not experience unconsciousness...

You know what? The findings in recent biology and the explorations in exo-biology are pointing to mysterious things. Some one will find something interesting soon--it will shake us up. Would you please point to some of the findings you refer to? I am very curious of what you refer to. And I am not ...

I do not go with flow. I follow the data. Optimally, that means going with the flow, but I do not allow myself to forget that deviant opinions is what has driven science forward. As long as such opinions are supported by scientific data, that is. Remember: the plural of anecdote is not data! The ris...

So possibly your cube was modified to UV. The XF06 is a UV set. Easy to check the emission filter. If it is the 450DF65, it should pass blue light. If Olympus G, it should pass orange-red light (590nm and up). And the dichroic mirror: if it is the 400DCLP, it should reflect violet and pass green. I...

I can't get any light to come through.... I noticed the back of the cube is labeled "Omega XF06." This describes the following setup: Exciter: XF1005 (365WB50) Dichroic: XF2001 (400DCLP) Emitter: XF3002 (450DF65) as described here: http://www.glenspectra.co.uk/glen/filters/pdfs/XF06.pdf But the Olym...