MicrobeHunter.com Microscopy Forum

In an answer above I referenced a testate amoeba shot I intended to post, and that I would reference here as a continuation of the above discussion. Hopefully if you were interested you found it... but in case not this was the post: http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=6&t=9...

Appreciate the comments. Thanks. That is a thing of beauty. What type of magnification is used. Thanks This was shot with a 20/0.70 on my Olympus BHS. I used a 2.5X NFK photoeyepiece, and the DIC component adds another 1.25X. So the total magnification on the camera sensor was 62.5X. That puts the d...

Funny how one can look and not see. Just noticed that it has 12 evenly spaced and evenly sized nuclei. I did notice that and found it interesting, According to Ferry's terrific site: https://www.arcella.nl/arcella-megastoma/ if it is Arcella megastoma it could have between 2-200 nuclei! Indeed, one...

Here is an Arcella sp. (Perhaps Arcella megastoma). I had a sample that contained quite a few of these so I spent time looking and collecting ones that had the most transparent shell. I photographed a "full stack" of images. For the first image I performed stacking operations that emphasized and reg...

This discussion also made me think of your flash-illuminated photos of bdelloid rotifers etc. These must nearly always be single shots, right? Somehow I never get the feeling that I want to "fine-focus" up and down from those. I guess the key is to focus on the exact right spots to keep the eyes fr...

great images. When highly polished images show up on this forum, it is always a question of how much the final presented image relates to the viewed image , at the point the image was captured. Most microscopists spend 99.9% of their microscope time viewing through the microscope and perhaps the re...

The first two diatoms were found in an old sample jar I had left outside for a few months. The last one is from a recent marsh sample. 40/0.95 Olympus S Plan Apo. 2.5X NFK photoeyepiece. Canon EOS R. DIC. http://www.krebsmicro.com/forumpix-microbhunt/Q6A1527B-1024.jpg 100/1.40 Olympus S Plan Apo. 2....

Do like the casting rod idea. It makes using a plankton net a much more viable proposition. Thanks Yes it's worked out well. But you do get some strange looks from people wanting to know what you are fishing for ;-) I once even had a fish & wildlife enforcement officer come up to me wondering what ...

Question... Do you have a base light. Can you do a "conventional" brightfield image? If so, do you get a clean image ("no doubling") when all DIC components and other filters are out of the light path? I doubt this is your problem, but I once had a BH2 vertical illuminator that gave me faint double ...

Thanks for the replies. My plankton net has an 80 micron mesh. I use it with a fishing pole off, and generally work with it off fishing piers or boating docks.

Made a couple of trips to Puget Sound. Here are a few of the resulting images. The first two, and the foraminifer are from algae collected at a low tide. The second and third were collected with a plankton net 10X objective with 2.5X NFK. Darkfield. http://www.krebsmicro.com/forumpix-microbhunt/Q6A1...

This objective was the S Plan Apo 100X in the Olympus biological BH series objectives. I won't say it was very common but it certainly was readily available. Generally there are always a few on Ebay. I don't use it as much after I obtained a 60/1.40, but the diaphragm can occasionally be useful for ...

Apparently these guys didn't get the message from their governor about social distancing these days. I collected some alga during a low tide and as I was leaving I noticed a dark brown "slimey" mass about the sixe of a tennis ball floating a few feet out in the water. My suspicions were verified as ...

Really nice images!

Not 100% sure, but don't think so. the CX-PCD attached to the microscope via a collar around the top of the condenser. Pretty sure all BX series attach via a dovetail at the base of the condenser.

The oil would be a safe way to go with the 0.75. Maybe I'm getting a bit lazy, but I try to avoid oiled darkfield unless absolutely needed. Nikon makes an excellent dry darkfield condenser...0.95-0.8. I use it regularly with a 20/0.70 Olympus Plan Apo where it works great. Also works great with 40/0...

Definitely a rotifer. Possible Lecane sp.

Charlie, I have seen a fair number of tardigrades with the inclusions you have shown us. The most amazing example was about the time I started using a microscope (as an adult) about 16 years ago. Have a look at this one. It really looked like a plastic bag filled with ice cubes. It moved about norma...

We're all familiar with dandelion seed parachutes, and to the naked eye our sense of them is something soft and fuzzy. One fascinating thing about microscopy is how such a familiar subject is transformed when we see the details at high magnifications. The bristles (to me anyway) take on the appearan...

Excellent. IMO this is right up there at the "bar".

with a (Canon) DSLR body suspended over an objective, but I would like a bit more mag. ! Please be a little more specific about how you are currently set up. I know you said you are contemplating an upgrade, but what do you have "in hand" now, and how are you using it? Generally there are a few way...

Charles, what is your experience, does the SLR modes (thats avoids the initial double-shutter action) provide actual benefit or better results? For example if the organism reaactes sensible on vibrations? I also using the SLR mode but it is maybe more a psychological thing and preference. I suppose...

Michael,
I was afraid you might ask about that... I'm just not the tethering type!

There's no IR remote. And I have no idea about a second method or triggering when connected to EOS Utilities.

There are so many options these days. As to magnification 10-20X is a good start. A rough idea of budget would be good to get suggestions. One thing I will mention. Stereo is great, but it takes a little effort to get the interpupillary distance right with a 5 year old. She might get it right away, ...

Don't know what it is, but to me it sure looks like Brownian motion (rather than bacteria or little critters)... very small particles moving randomly after collisions with molecules in the liquid. (In out microscopes we commonly see this in Closterium, where, at the tips, crystals of barium or calci...

I read with interest the reasons Charles gave for selecting the 90D. Does it suffer from the vibration issues that plagued the 60D and were still evident to a lesser degree in to 70D and 80D models despite them having EFCS? When used as a DSLR (not in "live-view") it behaves pretty much the same as...

The truth is I sometimes want to mix below stage light with top stage light. I call it twilight. Absolutely! You can do all sorts of background colors/patterns, or additional "rim" lighting from below. But I think the first thing to do is establish your top lighting first. When satisfied and comfor...

Great looking diatoms.
They're easing up access here a bit starting tomorrow, so I hope to get some salt water samples this week myself!

Don, The Ikea JANSJÖ light you have is very popular one for macro/micro work. But I think the idea of using a small aperture on it is not the way to go. You should be able to get pretty good "top-light illuminated" darkfield with the 4X and 10X with that light. It's going to be more difficult with t...

A couple questions: What is the light source? What objective are you using? ( Very Roughly) How much working distance do you figure you have? (Distance between tip of objective and the subject) Coverglass over subject or no coverglass? (Really does not matter with the 4X or 10X. Not having a covergl...