MicrobeHunter.com Microscopy Forum

With reflected brightfield epi illumination of highly reflective flat surfaces (like wafers) it is going to nearly impossible to avoid serious glare issues. Polarization would help a great deal. You need 2 polarizers, one between the light and the subject. Another between the subject and eyepieces/c...

Great looking video! Beautiful subject!

Beautiful images. Well Done!

billporter1456's approach is good and typical for collection. If there is a great deal of loose vegetation at your collection site is is also a good idea to lift up clumps of the vegetation and let it drip into your collection jar. In addition, it is extremely helpful to have a stereo microscope to ...

Thanks for the kind comments. What technique for the rotifer? All these are DIC, with a "basic" wet mount. One of the more tricky aspects with the rotifers is that often they "sway" back and forth and up and down while "feeding". Since the NA of the objectives used is pretty high (0.70 for the 20X a...

Hello again all. Was reading through the various "boards", learned a few new things and thought I would contribute a few new images. I haven't had much scope time this summer but I kept an outdoor "tank" which proved to be decidedly non-productive. But I did find a few nice subjects Here is a Rotari...

If you are not familiar with the work of Joseph Leidy and his book from 1879... 'Fresh-Water Rhizopods of North America', you are in for a treat. An amazing piece of work from 138 years ago! Overall, check out this: https://user.xmission.com/~psneeley/Personal/Leidy.htm And for the particular image ...

As has been mentioned, the "intent" of the image will dictate a good deal of how much to clean it up. A purely "scientific" intent verses a purely "aesthetic" intent will receive different treatments. I think a great many of the images we like fall somewhere in the middle. I tend to be very careful ...

The actual light producing component in a single LED is physically quite small. The smaller the light source, the harsher and more contrasty the light quality. So I can see why some feel there is more glare with an LED light if the light output is largely unmodified. But most of the time the light i...

Great! Some additional thoughts... If I am doing some work with my other objectives I can move the adapter about. Not perfect but reasonable. I would not entertain the idea of adjusting the adapter to accommodate an "oddball" objective that does not agree with your primary 5 objectives. I think it i...

Your 1200D has an APS-C sized sensor (22.3 x 14.9 mm for Canon). Ideally, if you are using 10X eyepieces on your microscope, you will want a magnification of about 1.5-1.7x from the trinocular tube into the camera body. Unfortunately the only ones I am aware of in that range are extraordinarily expe...

Oktagon, I'm slowly getting this Diaphot TMD set-up the way I want it. It is set up for DIC, and the condenser has a polarizer and swing-in wave plate, but when "in place" they can't be rotated. For the type of polarized shot posted above, it is really nice (probably necessary) to have the ability t...

Thank you for the very kind remarks. How about details as to technique and objectives. What magnifications and equipment were used? Is there any post processing of the images? Pretty straightforward. Subject between two polarizers, at least one of which can be rotated. A full wave plate was also pla...

Nice images. Always fun to watch these guys move along the coverslip!

This is great! Fascinating details!

Nice shots! The symmetry and "order" you find in nature under a microscope is so often intriguing.

They all look great. Something especially appealing to me about the second image.

This has turned into a very interesting thread. I like that Karl posted an example shot showing the direct FOV with a simple, empty tube that places the sensor right at the intermediate image location. I took that image and added some more info. If you were to add optics to the trinocular tube that ...

I will keep an eye out for any 1.4x tele-converters that i can throw inside the tube but for now i'm more than happy to crop in PP! I wouldn't mess with the tube itself at this point (except as JimT suggested... be sure there are no internal reflections or bright surfaces). It just appeared that yo...

Karl, Yes it looks good. I'm pretty sure that as you work more with this set-up you will indeed see overall better image quality. (BTW...a 40/0.80 is exactly the kind of objective where you really want to avoid significant focus change between viewing eyepieces and camera. What objective is that?) I...

OK... So when you say "tube lenses" in your previous answer... and had built in shutters and tube lenses connected to exposure control units. The tube lenses were adjustable for the camera format and offered a magnification of between 2.5x and 5X, depending on the format used ...you are referring to...

and had built in shutters and tube lenses connected to exposure control units. The tube lenses were adjustable for the camera format and offered a magnification of between 2.5x and 5X The distance from the correcting lens in the head is about to the sensor is around 19 cm. at parfocal. apochronaut,...

JimT has it. Cyanobacteria (blue-green algae).

I suspect lorez is correct, in that repair is needed. These folks were able to repair a faulty power supply on an Olympus BHS for me. It was under "guarantee" by the dealer, l so I don't know the cost involved (you might find it is too high to justify). Rocky Mountain Microscope Corporation   440 S ...

I know that there is a better way to go about throwing my camera on the AO20 using a combination of a specific focusing photo eyepiece and photo tube for the Trinoc head but I'm only here for another 2 weeks then work gets in the way, so this quick fix is just to keep me going until I'm back from m...

Would i be right in assuming we want the best of both worlds...parfocal and a matching FOV? Yes, if possible that should be the goal (or at least as close as it is reasonably possible). Both are pretty important in the overall scheme of things if you are trying to maximize picture quality. Being re...

The hind legs of the water boatman are very specialized, enabling it to be a powerful swimmer. They have a variety of special hairs that pivot and flatten against the leg on the forward stroke to provide minimum resistance, and then pivot and flare open forming a paddle with a large "surface area" o...

This discussion seems to have split has split into two different (but related) paths. Through the eyepiece everything is crisp and sharp but via the camera/tablet its clunkier...the cell wall lines look thicker, it doesn't reach the same pinoint detail that i get through the eyepiece. I think Tom Jo...

I'm adding another post today since the primary point of the Campanella post was the interesting cilia motion that was "frozen" by using electronic flash. With this critter the cilia motion a a key attraction as well. This is a favorite creature for me. It is mesmerizing to watch when feeding, and t...