Search found 201 matches

by c-krebs
Mon Jun 28, 2021 4:10 am
Forum: Illumination Techniques
Topic: A question concerning resolution
Replies: 9
Views: 4767

Re: A question concerning resolution

Greg, Never tried anything like this nor am I familiar with the material you mentioned. But your post really makes me think of an opticians diagnostic tool called an "pinhole occluder". There are even pinhole eyeglasses available (stenopeic glasses) that some people find useful. If you do some searc...
by c-krebs
Wed Feb 24, 2021 7:07 am
Forum: Pictures and Videos
Topic: Midge antenna. Collembola
Replies: 0
Views: 862

Midge antenna. Collembola

Rather slim pickings this time of year around here for small outdoor creatures. But springtails and midges are still pretty common. These images (all "stacks") were made with a modified Nikon MM-11 scope and Olympus "M" type objectives. 5/0.15 MPLFLN http://www.krebsmicro.com/forumpix/4506-v2small.j...
by c-krebs
Sun Feb 21, 2021 12:43 am
Forum: Microscopes and optics
Topic: Does it make sense to use Nikon CFN lenses on a olympus BH-2?
Replies: 18
Views: 6206

Re: Does it make sense to use Nikon CFN lenses on a olympus BH-2?

You're asking all the right questions. And you have already gotten some good into. I'll try to add a little more to the mix. You didn't mention what camera you are using other than it is an APS-C format. If it is a DSLR it will be extremely difficult to do "direct projection" (if even possible) with...
by c-krebs
Wed Feb 17, 2021 5:10 am
Forum: Pictures and Videos
Topic: Cladocera
Replies: 8
Views: 3090

Cladocera

10X darkfield
Image

20X DIC
Image

20X DIC
Image
by c-krebs
Tue Feb 16, 2021 6:33 am
Forum: Pictures and Videos
Topic: Chrysiridia rhipheus (Madagascan sunset moth)
Replies: 3
Views: 1564

Chrysiridia rhipheus (Madagascan sunset moth)

I can hardly count the number of times I've photographed this spectacular moth. Like many others, most of the time I concentrated on the obvious... the dramatic iridescent scales seen in the first image. This time I thought I would look more at some backlit scales that were more "pedestrian", lackin...
by c-krebs
Tue Jun 09, 2020 7:52 am
Forum: Pictures and Videos
Topic: Fresh water diatoms
Replies: 19
Views: 8343

Re: Fresh water diatoms

In an answer above I referenced a testate amoeba shot I intended to post, and that I would reference here as a continuation of the above discussion. Hopefully if you were interested you found it... but in case not this was the post: http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=6&t=9...
by c-krebs
Fri Jun 05, 2020 11:49 pm
Forum: Pictures and Videos
Topic: Arcella sp., Two different views from 1 stack
Replies: 12
Views: 6348

Re: Arcella sp., Two different views from 1 stack

Appreciate the comments. Thanks. That is a thing of beauty. What type of magnification is used. Thanks This was shot with a 20/0.70 on my Olympus BHS. I used a 2.5X NFK photoeyepiece, and the DIC component adds another 1.25X. So the total magnification on the camera sensor was 62.5X. That puts the d...
by c-krebs
Wed Jun 03, 2020 9:59 pm
Forum: Pictures and Videos
Topic: Arcella sp., Two different views from 1 stack
Replies: 12
Views: 6348

Re: Arcella sp., Two different views from 1 stack

Funny how one can look and not see. Just noticed that it has 12 evenly spaced and evenly sized nuclei. I did notice that and found it interesting, According to Ferry's terrific site: https://www.arcella.nl/arcella-megastoma/ if it is Arcella megastoma it could have between 2-200 nuclei! Indeed, one...
by c-krebs
Tue Jun 02, 2020 12:34 am
Forum: Pictures and Videos
Topic: Arcella sp., Two different views from 1 stack
Replies: 12
Views: 6348

Arcella sp., Two different views from 1 stack

Here is an Arcella sp. (Perhaps Arcella megastoma). I had a sample that contained quite a few of these so I spent time looking and collecting ones that had the most transparent shell. I photographed a "full stack" of images. For the first image I performed stacking operations that emphasized and reg...
by c-krebs
Mon Jun 01, 2020 11:53 pm
Forum: Pictures and Videos
Topic: Fresh water diatoms
Replies: 19
Views: 8343

Re: Fresh water diatoms

This discussion also made me think of your flash-illuminated photos of bdelloid rotifers etc. These must nearly always be single shots, right? Somehow I never get the feeling that I want to "fine-focus" up and down from those. I guess the key is to focus on the exact right spots to keep the eyes fr...
by c-krebs
Mon Jun 01, 2020 9:18 pm
Forum: Pictures and Videos
Topic: Fresh water diatoms
Replies: 19
Views: 8343

Re: Fresh water diatoms

great images. When highly polished images show up on this forum, it is always a question of how much the final presented image relates to the viewed image , at the point the image was captured. Most microscopists spend 99.9% of their microscope time viewing through the microscope and perhaps the re...
by c-krebs
Mon Jun 01, 2020 7:14 am
Forum: Pictures and Videos
Topic: Fresh water diatoms
Replies: 19
Views: 8343

Fresh water diatoms

The first two diatoms were found in an old sample jar I had left outside for a few months. The last one is from a recent marsh sample. 40/0.95 Olympus S Plan Apo. 2.5X NFK photoeyepiece. Canon EOS R. DIC. http://www.krebsmicro.com/forumpix-microbhunt/Q6A1527B-1024.jpg 100/1.40 Olympus S Plan Apo. 2....
by c-krebs
Mon Jun 01, 2020 6:28 am
Forum: Pictures and Videos
Topic: Marine diatoms, foraminifer, copepod
Replies: 9
Views: 4119

Re: Marine diatoms, foraminifer, copepod

Do like the casting rod idea. It makes using a plankton net a much more viable proposition. Thanks Yes it's worked out well. But you do get some strange looks from people wanting to know what you are fishing for ;-) I once even had a fish & wildlife enforcement officer come up to me wondering what ...
by c-krebs
Mon Jun 01, 2020 6:22 am
Forum: Illumination Techniques
Topic: Epi DIC woes
Replies: 100
Views: 51139

Re: Epi DIC woes

Question... Do you have a base light. Can you do a "conventional" brightfield image? If so, do you get a clean image ("no doubling") when all DIC components and other filters are out of the light path? I doubt this is your problem, but I once had a BH2 vertical illuminator that gave me faint double ...
by c-krebs
Sun May 31, 2020 8:28 pm
Forum: Pictures and Videos
Topic: Marine diatoms, foraminifer, copepod
Replies: 9
Views: 4119

Re: Marine diatoms, foraminifer, copepod

Thanks for the replies. My plankton net has an 80 micron mesh. I use it with a fishing pole off, and generally work with it off fishing piers or boating docks.
Image
by c-krebs
Fri May 29, 2020 7:10 am
Forum: Pictures and Videos
Topic: Marine diatoms, foraminifer, copepod
Replies: 9
Views: 4119

Marine diatoms, foraminifer, copepod

Made a couple of trips to Puget Sound. Here are a few of the resulting images. The first two, and the foraminifer are from algae collected at a low tide. The second and third were collected with a plankton net 10X objective with 2.5X NFK. Darkfield. http://www.krebsmicro.com/forumpix-microbhunt/Q6A1...
by c-krebs
Thu May 21, 2020 8:33 pm
Forum: Microscopes and optics
Topic: Olympus Splan Apo 100x 1.4NA +coverslip +iris - The Objective that Doesn't Exist?
Replies: 8
Views: 3935

Re: Olympus Splan Apo 100x 1.4NA +coverslip +iris - The Objective that Doesn't Exist?

This objective was the S Plan Apo 100X in the Olympus biological BH series objectives. I won't say it was very common but it certainly was readily available. Generally there are always a few on Ebay. I don't use it as much after I obtained a 60/1.40, but the diaphragm can occasionally be useful for ...
by c-krebs
Thu May 21, 2020 7:09 pm
Forum: Pictures and Videos
Topic: Tube-dwelling diatoms. Social distance FAIL
Replies: 0
Views: 1078

Tube-dwelling diatoms. Social distance FAIL

Apparently these guys didn't get the message from their governor about social distancing these days. I collected some alga during a low tide and as I was leaving I noticed a dark brown "slimey" mass about the sixe of a tennis ball floating a few feet out in the water. My suspicions were verified as ...
by c-krebs
Thu May 21, 2020 6:39 pm
Forum: Pictures and Videos
Topic: mold
Replies: 9
Views: 4056

Re: mold

Really nice images!
by c-krebs
Wed May 13, 2020 1:16 am
Forum: Microscopy accessories
Topic: Will Olympus CX-PCD fit a BX?
Replies: 1
Views: 1905

Re: Will Olympus CX-PCD fit a BX?

Not 100% sure, but don't think so. the CX-PCD attached to the microscope via a collar around the top of the condenser. Pretty sure all BX series attach via a dovetail at the base of the condenser.
by c-krebs
Tue May 12, 2020 8:36 pm
Forum: Illumination Techniques
Topic: DF NA requirements
Replies: 4
Views: 3058

Re: DF NA requirements

The oil would be a safe way to go with the 0.75. Maybe I'm getting a bit lazy, but I try to avoid oiled darkfield unless absolutely needed. Nikon makes an excellent dry darkfield condenser...0.95-0.8. I use it regularly with a 20/0.70 Olympus Plan Apo where it works great. Also works great with 40/0...
by c-krebs
Mon May 11, 2020 3:11 am
Forum: Identification help
Topic: Unknown Crustacean
Replies: 2
Views: 2145

Re: Unknown Crustacean

Definitely a rotifer. Possible Lecane sp.
by c-krebs
Mon May 11, 2020 2:54 am
Forum: Pictures and Videos
Topic: spring'2020 bear hunt with questions'.
Replies: 27
Views: 10857

Re: spring'2020 bear hunt with questions'.

Charlie, I have seen a fair number of tardigrades with the inclusions you have shown us. The most amazing example was about the time I started using a microscope (as an adult) about 16 years ago. Have a look at this one. It really looked like a plastic bag filled with ice cubes. It moved about norma...
by c-krebs
Sun May 10, 2020 5:58 am
Forum: Pictures and Videos
Topic: Dandelion pappus ("parachute") details
Replies: 5
Views: 2694

Dandelion pappus ("parachute") details

We're all familiar with dandelion seed parachutes, and to the naked eye our sense of them is something soft and fuzzy. One fascinating thing about microscopy is how such a familiar subject is transformed when we see the details at high magnifications. The bristles (to me anyway) take on the appearan...
by c-krebs
Sun May 10, 2020 5:25 am
Forum: Pictures and Videos
Topic: Arachnoidiscus ehrenbergi
Replies: 16
Views: 6344

Re: Arachnoidiscus

Excellent. IMO this is right up there at the "bar".
by c-krebs
Thu May 07, 2020 8:09 pm
Forum: Beginner's corner
Topic: Eypiece projection - How much ?!
Replies: 2
Views: 2129

Re: Eypiece projection - How much ?!

with a (Canon) DSLR body suspended over an objective, but I would like a bit more mag. ! Please be a little more specific about how you are currently set up. I know you said you are contemplating an upgrade, but what do you have "in hand" now, and how are you using it? Generally there are a few way...
by c-krebs
Thu May 07, 2020 6:57 pm
Forum: Pictures and Videos
Topic: Micro stonemasons. Difflugia.
Replies: 21
Views: 8570

Re: Micro stonemasons. Difflugia.

Charles, what is your experience, does the SLR modes (thats avoids the initial double-shutter action) provide actual benefit or better results? For example if the organism reaactes sensible on vibrations? I also using the SLR mode but it is maybe more a psychological thing and preference. I suppose...
by c-krebs
Thu May 07, 2020 7:48 am
Forum: Pictures and Videos
Topic: Micro stonemasons. Difflugia.
Replies: 21
Views: 8570

Re: Micro stonemasons. Difflugia.

Michael,
I was afraid you might ask about that... I'm just not the tethering type! ;)

There's no IR remote. And I have no idea about a second method or triggering when connected to EOS Utilities.
by c-krebs
Thu May 07, 2020 7:17 am
Forum: Beginner's corner
Topic: Stereo microscope magnification comparison?
Replies: 21
Views: 7208

Re: Stereo microscope magnification comparison?

There are so many options these days. As to magnification 10-20X is a good start. A rough idea of budget would be good to get suggestions. One thing I will mention. Stereo is great, but it takes a little effort to get the interpupillary distance right with a 5 year old. She might get it right away, ...
by c-krebs
Thu May 07, 2020 6:54 am
Forum: Pictures and Videos
Topic: Another Video For ID
Replies: 3
Views: 2498

Re: Another Video For ID

Don't know what it is, but to me it sure looks like Brownian motion (rather than bacteria or little critters)... very small particles moving randomly after collisions with molecules in the liquid. (In out microscopes we commonly see this in Closterium, where, at the tips, crystals of barium or calci...