Search found 77 matches

by Topcode
Fri Apr 12, 2024 10:53 pm
Forum: Camera systems and imaging
Topic: What accessory do I need to par focal microscope camera in trinocular tube of stereo microscope ?
Replies: 13
Views: 899

Re: What accessory do I need to par focal microscope camera in trinocular tube of stereo microscope ?

Assuming that those adapters are the right threads/attachment, they should work just fine for your purposes. I have that exact same 1x one (though bought from aliexpress) and it works really well.
by Topcode
Fri Apr 05, 2024 1:48 pm
Forum: Microscopes and optics
Topic: Camera adapter Touptek FMA050 replacement to remove some chromatic aberration
Replies: 2
Views: 558

Re: Camera adapter Touptek FMA050 replacement to remove some chromatic aberration

Unless you can 3D print optical lenses, you will need to buy an apochromatic reducer, or use no reducer at all.
by Topcode
Mon Apr 01, 2024 6:03 am
Forum: Pictures and Videos
Topic: One of the first real results from my neophot 21
Replies: 0
Views: 929

One of the first real results from my neophot 21

My neophot 21 is still in quite the state of disarray, realistically at some point I need to do a complete disassembly and clean, but as it is now, it works, for some results. So, here's a look at the quad bayer filter from an ipad pro 12.9" 2015 front camera. (model a1652) https://i.imgur.com/1qqEC...
by Topcode
Sun Mar 31, 2024 3:45 am
Forum: Pictures and Videos
Topic: Everything looks better in slow motion
Replies: 12
Views: 1795

Re: Everything looks better in slow motion

More or less, tho the inverse is probably more accurate, as it gets larger, approaching 100% of the sensor, the fps gets lower.
by Topcode
Fri Mar 29, 2024 4:52 am
Forum: Pictures and Videos
Topic: Everything looks better in slow motion
Replies: 12
Views: 1795

Everything looks better in slow motion

I found this in a sample of water, mixed with pollen, which I let sit out for like a week. I then took a sample of pollen biofilm type thing, and put it on a slide. The results were worth it. No clue what this little guy is, BUT, I know that like everything, slow mo makes it better. https://i.imgur....
by Topcode
Tue Mar 19, 2024 12:12 am
Forum: Pictures and Videos
Topic: A set of videos from a marine sample from the Gulf of Thailand
Replies: 2
Views: 624

Re: A set of videos from a marine sample from the Gulf of Thailand

Your embeds are messed up, dont include the "https://youtu.be/" part

amazing videos nonetheless
by Topcode
Thu Mar 14, 2024 5:38 pm
Forum: Identification help
Topic: which sigmoid diatom is this?
Replies: 3
Views: 1201

Re: which sigmoid diatom is this?

Not sure if i can help with ID, but that is an absolutely beautiful diatom.
by Topcode
Thu Mar 14, 2024 4:19 am
Forum: Pictures and Videos
Topic: Euglenid posing as a hermit crab?
Replies: 7
Views: 587

Re: Euglenid posing as a hermit crab?

Deliberate or not, that guy is chilling in a little protective house, even if it don't know it, cool thing to see. Plus, if its more stationary, it can be easier to play around with lighting and capturing it.
by Topcode
Mon Mar 11, 2024 6:55 pm
Forum: Pictures and Videos
Topic: Two unique looking alage specimens
Replies: 1
Views: 195

Two unique looking alage specimens

Taken from the same sample as my two slow motion posts, a bottle of pond water sitting on a window sill. The "8" shaped guy is just about 20 micron long. And in the cluster, they are each about 14 micron wide or so. https://i.imgur.com/4UwoGa7.png I believe this one is a Pediastrum duplex, made up o...
by Topcode
Sun Mar 10, 2024 6:12 pm
Forum: Pictures and Videos
Topic: Ciliate at high framerates(1400+ fps)
Replies: 2
Views: 293

Re: Ciliate at high framerates(1400+ fps)

Very interesting! Thanks for sharing. So you’re getting these high frame rates by sampling only a small portion of the sensor for each frame? I’ll have to go back to your original post to try figure out what’s going on. Harry Yes, but it is slightly more complicated than just a smaller portion of t...
by Topcode
Sun Mar 10, 2024 8:09 am
Forum: Pictures and Videos
Topic: Ciliate at high framerates(1400+ fps)
Replies: 2
Views: 293

Ciliate at high framerates(1400+ fps)

A couple days back I provided some gifs of some Spirillum at high frame rates, but while it was neat to see them. They didn't really do too much interesting stuff. But now, I've got more, bigger too. This bad boy is some type of ciliate, with amazing patterns of motion up and down its body, in such ...
by Topcode
Tue Mar 05, 2024 3:22 pm
Forum: Digital processing
Topic: Chromatic aberrations filtering
Replies: 52
Views: 4944

Re: Chromatic aberrations filtering

It seems now blurry (in a crop), this is what I'd like to check using a variable length photo port tube: MGKgUV0.processed.jpg well, any CA will always leave you with some blur, because RGB are broadband, from one end of your camera picking up red(maybe, 700nm), to the other end(maybe, 600nm), ther...
by Topcode
Tue Mar 05, 2024 1:29 am
Forum: Digital processing
Topic: Chromatic aberrations filtering
Replies: 52
Views: 4944

Re: Chromatic aberrations filtering

but was I was talking about just shifts around the color channels to reduce some forms of chromatic aberration Their shift around solution seems clear to me. They drop all the blue channel, and rebuild an alternate using the red and green channels. Yes, that's what your video describes. What I am d...
by Topcode
Mon Mar 04, 2024 11:39 pm
Forum: Digital processing
Topic: Chromatic aberrations filtering
Replies: 52
Views: 4944

Re: Chromatic aberrations filtering

SerPlayer... I see not not much info about it using Google. Brought me to SharpCap, a solution embedding it. I'm starting to watch this video. Seems they exclude part of the color spectrum, to rebuild colorful pictures without aberrations, with a "synthetic blue channel", "preventing defocused blue...
by Topcode
Mon Mar 04, 2024 10:01 pm
Forum: Digital processing
Topic: Chromatic aberrations filtering
Replies: 52
Views: 4944

Re: Chromatic aberrations filtering

Well for videos, I have used SerPlayer, but that again, really only for lateral CA. Which it does look like you have some(in addition to the axial/focal CA), but it doesnt scale, only moves, so, its not that useful unless you have a specific target in the frame. But it was used for some of my stuff,...
by Topcode
Mon Mar 04, 2024 7:53 pm
Forum: Digital processing
Topic: Chromatic aberrations filtering
Replies: 52
Views: 4944

Re: Chromatic aberrations filtering

Well, if you experience lateral chromatic aberration, I have found that using RegiStax can help somewhat, but it seems like you mostly have issues with axial chromatic aberration.
by Topcode
Mon Mar 04, 2024 6:03 am
Forum: Pictures and Videos
Topic: High frame rate with a mobile phone : a Vorticella
Replies: 1
Views: 243

Re: High frame rate with a mobile phone : a Vorticella

That's nice! I haven't actually ever found any vorticella, so its pretty cool to see them slowed down.
by Topcode
Sat Mar 02, 2024 9:53 pm
Forum: Pictures and Videos
Topic: Various Spiral bacteria at high framerates(1400+)
Replies: 18
Views: 1161

Re: Various Spiral bacteria at high framerates(1400+)

Only if the fancy strikes you, 'metachronal beat' of rotifer coronal cilia 'wheels' would be sweet with your high framerate application, so too would the waves of coordinated bands of cilia on many protozoa. Funny you should mention that, I did do rotifers a while back. https://www.youtube.com/watc...
by Topcode
Sat Mar 02, 2024 9:27 pm
Forum: Pictures and Videos
Topic: Various Spiral bacteria at high framerates(1400+)
Replies: 18
Views: 1161

Re: Various Spiral bacteria at high framerates(1400+)

Cropping does not increase frame rates. yes, its the roi set to 1488x76(100% of the horizonat resolution at bin2, and 1/20th of the vertical resolution at bin2) that increases the framerate, not the cropping of the horizontal distance. This is because of the way cameras are driven, speed through ve...
by Topcode
Sat Mar 02, 2024 8:51 pm
Forum: Pictures and Videos
Topic: Various Spiral bacteria at high framerates(1400+)
Replies: 18
Views: 1161

Re: Various Spiral bacteria at high framerates(1400+)

The quoted specs of your touptek camera say "Ultra-fine Color hardware Color Enginne ensuring high frame rates(Up to 15 frames for 20M Resolution);" 15 frames is a bit away from 1450 frames. Do the math how low you must go with resolution to achieve 1450 fps. And yes, I don't know what I am speakin...
by Topcode
Sat Mar 02, 2024 8:04 pm
Forum: Pictures and Videos
Topic: Various Spiral bacteria at high framerates(1400+)
Replies: 18
Views: 1161

Re: Various Spiral bacteria at high framerates(1400+)

Your camera and its USB connection would not do 100 FPS. [citation needed] Here, ill provide mine. Here are the specs for the camera series I have from the manufacturer http://www.touptek.com/product/showproduct.php?lang=en&id=288 we can clearly see the model of camera I have listed as an IMX533 he...
by Topcode
Sat Mar 02, 2024 5:36 pm
Forum: Pictures and Videos
Topic: Various Spiral bacteria at high framerates(1400+)
Replies: 18
Views: 1161

Re: Various Spiral bacteria at high framerates(1400+)

Alexander wrote:
Sat Mar 02, 2024 4:50 pm
Topcode wrote:
Sat Mar 02, 2024 3:53 pm
Alexander wrote:
Sat Mar 02, 2024 9:57 am
You are confusing exposure time with frame rate.
No? The framerate means that roughly 1450 frames were captured every second, exposure time was about 60 microseconds by my recollection.
How could you expose 1450 times 60 milliseconds in one second?
Microseconds, not milliseconds.
by Topcode
Sat Mar 02, 2024 3:53 pm
Forum: Pictures and Videos
Topic: Various Spiral bacteria at high framerates(1400+)
Replies: 18
Views: 1161

Re: Various Spiral bacteria at high framerates(1400+)

You are confusing exposure time with frame rate. No? The framerate means that roughly 1450 frames were captured every second, exposure time was about 60 microseconds by my recollection. I can't see a full image--just a small preview and no motion. Hmm, they are all relatively small (<20mb) gif file...
by Topcode
Sat Mar 02, 2024 8:13 am
Forum: Pictures and Videos
Topic: Various Spiral bacteria at high framerates(1400+)
Replies: 18
Views: 1161

Re: Various Spiral bacteria at high framerates(1400+)

Thanks, topcode, can you show a few images of your setup? What sort of water sample, from what source did you collect these protists? What illumination permits: " 1400+ high framerates" ? Again, thanks for this microscopy. charlie g https://i.imgur.com/Ynp0zlQ.jpeg Yeah here is an image of the setu...
by Topcode
Sat Mar 02, 2024 1:22 am
Forum: Pictures and Videos
Topic: Various Spiral bacteria at high framerates(1400+)
Replies: 18
Views: 1161

Various Spiral bacteria at high framerates(1400+)

https://cdn.discordapp.com/attachments/1140084905930407987/1213278558114422855/240301185838_pipp_F4325-5000.gif?ex=65f4e4b5&is=65e26fb5&hm=e43d1001c5359413f723bc5784f6153247ad3a2d743b3ec8c9af1e8fa2acc468 https://cdn.discordapp.com/attachments/1199439657537110128/1213284526306889788/240301185838_pip...
by Topcode
Sun Feb 11, 2024 9:40 pm
Forum: Camera systems and imaging
Topic: Recommendation Camera for Zeiss Axiovert 25CFL with 1x Adapter
Replies: 11
Views: 1997

Re: Recommendation Camera for Zeiss Axiovert 25CFL with 1x Adapter

source? Read sources carefully. Did you find any proof that the posted data of IMX 585 was at 400.000 ISO? What are the sensitivity and noise level of the D6 at 100 ISO? Just do the test. Compare the cameras in real live not in sales literature. It was not at 400k iso, as dedicated cameras are not ...
by Topcode
Sun Feb 11, 2024 7:39 pm
Forum: Camera systems and imaging
Topic: Recommendation Camera for Zeiss Axiovert 25CFL with 1x Adapter
Replies: 11
Views: 1997

Re: Recommendation Camera for Zeiss Axiovert 25CFL with 1x Adapter

Exactly. But a consumer DSLR is much more sensitive than 99 % of all dedicated microscope cameras. source? For example, the Nikon D6 based on data here has the same read noise at 400000 ISO, as an imx 585 does based on data here (yes thats for an astronomy camera, but some microscope cameras use id...
by Topcode
Fri Feb 09, 2024 10:19 pm
Forum: Microscopes and optics
Topic: Higher power eyepieces beyond 10x?
Replies: 6
Views: 792

Re: Higher power eyepieces beyond 10x?

Generally, I would recommend avoiding such eyepieces. They are typically not super useful, unless you have an odd higher NA objective with a lower magnification.
by Topcode
Mon Feb 05, 2024 7:44 pm
Forum: Digital processing
Topic: Why is photo sharpening necessary
Replies: 7
Views: 4757

Re: Why is photo sharpening necessary

blind deconvolution Thank you for providing the term for this. Now I’m wondering whether better stacking software has settings for this. https://en.wikipedia.org/wiki/Blind_deconvolution May i recommend the use of registax? Its intended mainly for planetary astrophotography, but ive used it for cor...
by Topcode
Sun Feb 04, 2024 8:14 pm
Forum: Microscopes and optics
Topic: Stereoscope vs. Compound Microscope at Given Total Magnification
Replies: 6
Views: 993

Re: Stereoscope vs. Compound Microscope at Given Total Magnification

Its kinda almost too general of a question to answer super well. A stereoscope can be much better than, or it can be way worse than a compound microscope. If you make everything the same specs to have an accurate comparison, then you arent very likely to have any notable differences, but that kinda ...