MicrobeHunter.com Microscopy Forum

In an attempt to create a Raman microscope spectrometer (or to add Raman spectroscopy to my PMIII) microscope, I have been putting a lot of effort into evaluating eyepiece/objective combinations to better understand the compensation or correcting values. I have posted some of this work in the past t...

I've continued to work on this project and wanted to post an update. After much experimenting I came to the conclusion that the problem was alignment, although I could not rule out issues with the microscope optics. I took a side tangent and spent a number of months aligning the PMIII, testing vario...

Thank you MicroBob, I was very curious about the hue differences on the actual photos versus Sony. I was curious how the printing process now works particularly how you also received a CD with the photos. Your explanation answers many of my questions. I did go back and scan the photos that came with...

I use a Sony a6000 with a Canon Photomicro Unit F microscope system and a e-mount to FD adapter. Sony has a mini HDMI out that make is super simple to connect to a monitor. In addition, the view is "live" so that what you see is what you get. I have been very please with the setup.

In recent attempts to achieve theoretical resolution on the microscope, all aspects of the microscope subsystems are being tested and optimized. This optimization has also included the best possible camera setup to image what is seen in the binoc eyepiece. It was of interest to compare the acquisiti...

Thanks for the responses. I was using a s second delay with a wireless remote on the camera. I'm not getting the resolution I think I should, but not sure why. These were all focal using a Canon Micro F connection system. I'm waiting on a 35mm lens that should arrive this week. I'll try the afocal m...

Actual pictures are much better resolved than what is posted due to reduced image resolution.

Second set Hoffman modulation and Phase Contrast. I could not get good results with DIC??

I have been trying to learn the true improvements that are gained by neofluar and apochromat objectives and corrected eyepieces. Yesterday great suggestions from the "microscope and optics" section of the forum led to some great reading and more pictures this afternoon of Kemp's Diatom 8 test plate,...

Thanks to all - I appreciate the comments. Given the suggestions and readings, I worked a little with enhancement techniques I had lying around. Posted photos in the specimen section of the forum.

Thanks again for all the very good information!

Mark

Very interesting idea with the histogram - well worth a try. I recently read an old article (can't find it in my computer mess right now) about how you could evaluate the correction of an objective by looking at a conoscopic diffraction image of an objective focused on a stage micrometer. After read...

Thanks for your responses! Camera setup was afocal using a Sony a6000 with Canon FD converter and attached to a Canon Photomicro Unit F adapter. I was using a Zeiss KPL 10W eyepiece in the camera adapter which only views about 40% of what is seen in the eyepiece. I posted a picture of the setup shwo...

As noted in the update comment in my earlier post titled "Questions about Zeiss Correcting Oculars", I am ultimately interested in learning of the best camera setup for the Zeiss Photomic. This has led me to an investigation in eyepiece/objective paring and several focal and afocal camera setups, an...

Apologies to keep bugging everyone with this, but I wasn't satisfied with my last posting. I knew that my PM was not optimally aligned and I was confident that there were artifacts in the images due to alignment. So, I followed Paul James method of aligning the PM Headrig (Mic-UK) and performed a th...

Thanks to everyone again who replied. I used the information given and ran tests on all the 40X objectives and eyepieces that I had available. Summary results first and then details to follow. Obviously, the best combination was the Zeiss KPL and Neofluar objective, followed by the Zeiss CPL and sam...

Thank you all for the replies, exactly what I was needing to know. After posting, I reviewed a copy of Zeiss Optical Systems, and found in section 72 the following: "C-type eyepieces may be used in conjunction with simple types of objective. Objectives of higher correction should, if possible, alway...

I recently wanted to upgrade my eyepieces from the Nikon CFW to Zeiss corrected eyepieces to see if there was any difference between the corrections noted. I posted another question asking for help discerning the differences.) However, I found that the new Zeiss eyepieces did not have rubber eye cup...

I would like to ask for help answering a question about correcting eyepieces. I have several sets of Zeiss and Nikon eyepieces (see photo), and an Olympus Photo eyepiece and would like to see if someone could direct me to documentation explaining the difference between the KPL and CFL specifically (...

I purchased a used HM 40X objective and condenser many years ago and rather like the contrast enhancement that I can get. I have reflected DIC and it is by far superior, but for transmission, my only enhancement options are darkfield, phase, HM, Rheinberg, or something I found on the internet called...

I recently saw on the internet an interesting article with microscope images of CD tracks. Given the challenges, I thought this an interesting experiment. The images (attached PDF file) for the CD and Blue-ray were computer screen captures to reduce size, but otherwise are directly from the camera w...

Thank you very much for your insight and feedback. I am eager to hear your results on your CREE LEDs. Based on comments here, I completed some more testing on how LEDs affect the color observed by using a LOMO monochromator to select color ranges over the spectrum. I will post that data soon. I also...

The Zeiss Lamp House adapter: I have not had the chance to play with one of these and was wondering if and how it compensated for the additional distance of the bulbs in the lighthouses attached to it compared to connecting the lighthouse directly to the connecting tube. I take it you can still foc...

For an amateur forum like this one, I would suggest adding a few summary lines, in addition to that long article. Your high power LEDs seem to have pretty good green output. What are their CRI values? Thank you for the feedback. Definitely agree, I should have included a summary - will make that a ...

I decided to post my experience with the Lomo Monochromator for the PoLam, when attached to a Zeiss Photomicroscope III. The summary is below and the full experience is attached. Curious, has any one else worked with this monochromator, would enjoy hearing your experience! Summary: The Lomo Polam Mo...

I have read a lot about LED retrofits for microscopes but have been very skeptical of power and color quality. I finally bit the bullet and did the work to see how some direct-replacement LEDs compared to the original halogen source in my photomic. What has been missing in much of the reports is spe...

I admit, this is more challenging that I had originally thought. I have added a dual mirror system to bring the LASER into the microscope which allows me to adjust the beam for better control. That has helped significantly. I have also tried various long pass filters that are affordable on a home bu...

I have added a green laser to my Zeiss photomic III to create a Raman microscope. I am finding alignment super critical. I have seen the article on aligning the photomic (head, nosepiece, field diaphragm, etc.), but I don't think I have it as good as it needs to be. Does anyone have knowledge of how...