Search found 3006 matches
- Thu Apr 18, 2024 9:35 pm
- Forum: Microscopes and optics
- Topic: Partially blocked Leica objective
- Replies: 14
- Views: 290
Re: Partially blocked Leica objective
Wayne - that "contrast automatic" feature is new to me. Thanks - it should be much easier to use than fiddling with perceived resolution and refocusing. My question - do you know if Nikon and Olympus infinite objectives behave in much the same way?
- Thu Apr 18, 2024 12:11 am
- Forum: Microscopes and optics
- Topic: Partially blocked Leica objective
- Replies: 14
- Views: 290
Re: Partially blocked Leica objective
+3 It's a Modulation Contrast objective, especially useful to add contrast to live specimens on an inverted microscope. Someone will likely be willing to swap it for one without the added bits of polarizing film. You might also hope to acquire a matching condenser and a couple more objectives - at a...
- Tue Apr 16, 2024 12:26 am
- Forum: Illumination Techniques
- Topic: My first impressions of DIC on the BX system and BX prism compatibility
- Replies: 3
- Views: 172
Re: My first impressions of DIC on the BX system and BX prism compatibility
These days, admission to an art museum is $20. Owning great art can be orders of magnitude more expensive. Your BX is like having lifetime rights to a spectacular collection—actually collected, curated, and often at least partly created or arranged by you.
"Wow" is an apt description.
"Wow" is an apt description.
- Mon Apr 15, 2024 6:40 pm
- Forum: Microscopes and optics
- Topic: how scared should I be of mercury lamps
- Replies: 14
- Views: 419
Re: how scared should I be of mercury lamps
There are two main risks: exposure to UV light in use and shattering of the bulb . . . with a release of mercury and mercury vapor. Unless the power supply shows a clear indication of the life left in the bulb or other reliable assurances it is near new, you might assume it's past its useful life an...
- Mon Apr 15, 2024 2:32 am
- Forum: Beginner's corner
- Topic: What happened to the Leica DMLB?
- Replies: 11
- Views: 969
- Sun Apr 14, 2024 7:34 pm
- Forum: Illumination Techniques
- Topic: Prepared slide set for demonstrating contrast techniques?
- Replies: 4
- Views: 281
Re: Prepared slide set for demonstrating contrast techniques?
You may want to show how the thickness of specimen preparation affects contrast methods as well. Stains also affect the choices. This will be easier and more beneficial with prepared specimens - so if most everything you're doing is unstained live protists and plankton it is perhaps not as relevant....
- Thu Apr 04, 2024 6:03 pm
- Forum: Microscopes and optics
- Topic: Leica DMRB- which lenses and prisms for DIC
- Replies: 12
- Views: 629
Re: Leica DMRB- which lenses and prisms for DIC
The Accu-Scope objectives on this are semi-apo. In other words, more like plan fluorite than plan apo. The ones I've had did not perform as well as their Olympus, Nikon, and Leica counterparts. They were better than plan achromats but not as good as the better plan fluorites from major makers. They'...
- Thu Apr 04, 2024 4:15 am
- Forum: Microscopes and optics
- Topic: Leica DMRB- which lenses and prisms for DIC
- Replies: 12
- Views: 629
Re: Leica DMRB- which lenses and prisms for DIC
As I understand it the difference between B and B1 or D and D1 (etc.) prisms is in the shear angles. The letter alone (A, B, C, D . . .) is a balance between 3D effect and ultimate resolution. The B1 (etc.) designation (as I understand it) has a bit less shear, a bit less contrast and 3D effect, but...
- Thu Apr 04, 2024 4:04 am
- Forum: Microscopes and optics
- Topic: Cleaning Fungus from Microscope Optics
- Replies: 5
- Views: 563
Re: Cleaning Fungus from Microscope Optics
If you search this site, you'll find some answers. Search is typically easiest if you Google something like "cleaning fungus from lenses site:microbehunter.com"
- Thu Apr 04, 2024 1:08 am
- Forum: Microscopes and optics
- Topic: Leica DMRB- which lenses and prisms for DIC
- Replies: 12
- Views: 629
Re: Leica DMRB- which lenses and prisms for DIC
The first eBay listing for the 10 PL Fluotar looks good. I believe it wants a K2. They do show up now and then. I suspect, but don't know, that the 10x Plan Apo wants a K2 as well. Note that you're not getting higher resolution than the same .30 N.A. Plan Fluor. You get slightly better correction of...
- Wed Apr 03, 2024 9:57 pm
- Forum: Microscopes and optics
- Topic: Leica DMRB- which lenses and prisms for DIC
- Replies: 12
- Views: 629
Re: Leica DMRB- which lenses and prisms for DIC
The older "Delta" optic type DMRB has black lettering - like yours. Unmarked Leica objectives and those with an HCX will match the tube lens a bit better. You do want objectives that specify a letter (A,B,C,D...) on the objective or very early on a * to be compatible with DIC. If you find an "HC" ob...
- Tue Apr 02, 2024 10:25 pm
- Forum: Microscopes and optics
- Topic: Averaging condenser and objective numerical aperture when predicting resolution
- Replies: 49
- Views: 3601
Re: Averaging condenser and objective numerical aperture when predicting resolution
The long-standing relationship of numerical aperture relationship to resolution is basically a geometric one - assuming every photon of light at some wavelength/energy is fully constrained within some cone of light. By that simple math, and using ray tracing, I think we all agree that (whether it is...
- Mon Apr 01, 2024 1:52 am
- Forum: Microscopes and optics
- Topic: Averaging condenser and objective numerical aperture when predicting resolution
- Replies: 49
- Views: 3601
Re: Averaging condenser and objective numerical aperture when predicting resolution
Thanks for taking the time to dig up those sources, Hans.
We both likely suspect the answer gets even more interesting if the subject isn't a grating, but something like a stained tissue.
We both likely suspect the answer gets even more interesting if the subject isn't a grating, but something like a stained tissue.
- Sun Mar 31, 2024 7:11 pm
- Forum: For forum members who want to buy and sell equipment
- Topic: Searching For: Extention between intermediate tube and the observation tube for an IMT-2 Olympus Inverted Microscope
- Replies: 4
- Views: 566
Re: Searching For: Extention between intermediate tube and the observation tube for an IMT-2 Olympus Inverted Microscope
Frank - here's a guess about your options (assuming I understand correctly that you want to raise the height of your eyepieces). The picture you show is a bit confusing to me - it looks like it is sitting above some sort of camera (?) adapter (that numbered dial on its upper left side) rather than d...
- Sat Mar 30, 2024 7:17 am
- Forum: Microscopes and optics
- Topic: Do I need another objective?
- Replies: 61
- Views: 3719
Re: Do I need another objective?
. . . I've heard (from Stephen, on this site) that the PF ELWD 60x is about as good as it gets for this objective and expect to have one fairly soon. . . . Going back a bit in this thread -- I can confirm that the PF ELWD 60C prism works very well with Nikon's dry 0.85na Plan Fluor objective - far ...
- Fri Mar 29, 2024 9:36 pm
- Forum: Microscopes and optics
- Topic: Olympus IMT-2 mystery
- Replies: 17
- Views: 1394
Re: Olympus IMT-2 mystery
Michael, the rating plate on your microscope suggests the power supply can support 100 watt lamps (100+ volts x 2A = 200 or so watts input). I've only seen versions of the IMT-2 with that 50-watt lamp housing. But, odds are, you're OK.
- Fri Mar 29, 2024 6:49 pm
- Forum: Microscopes and optics
- Topic: Olympus IMT-2 mystery
- Replies: 17
- Views: 1394
Re: Olympus IMT-2 mystery
Michael, I suspect your scope should have the black metal 50-watt halogen illuminator - and that someone switched lamp housings along the way. If you check the electrical plate for your scope, it may shed light (metaphorical kind) on what lamp wattage the power supply can reliably support. If the in...
- Fri Mar 29, 2024 6:11 am
- Forum: Microscopes and optics
- Topic: Olympus X-Line Objectives
- Replies: 6
- Views: 982
Re: Olympus X-Line Objectives
To me, the video sounds like the Evident marketing department just discovered "something called aberration" and decided they'd convince customers (who they apparently think need to be informed about "something called aberration") that their new private equity owners are doing something about it. Who...
- Fri Mar 29, 2024 1:06 am
- Forum: Microscopes and optics
- Topic: Field number vs. field stop diameter in "negative" eyepieces
- Replies: 8
- Views: 701
Re: Field number vs. field stop diameter in "negative" eyepieces
This may be QWERTY vs. Dvorak keyboards. Having gotten used to field numbers, FN certainly eems simple enough. Use a 10x/22 eyepiece and I expect to see a 22mm wide field with a 1x stereo objective and a 2.2mm field with a 10x compound objective. Put a ruler or micrometer slide down and take a look....
- Fri Mar 29, 2024 12:10 am
- Forum: Microscopes and optics
- Topic: Do I need another objective?
- Replies: 61
- Views: 3719
Re: Do I need another objective?
Fair point about objective quality. In my experience, the cheaper Chinese 60x dry plan achromats with no correction collar are commonly a disappointment. I'd rather add a drop of oil to a 50-60-63-100x plan achromat oil objective. The 50x oil immersion finite objectives from Nikon and Olympus with a...
- Thu Mar 28, 2024 11:27 pm
- Forum: Microscopes and optics
- Topic: Do I need another objective?
- Replies: 61
- Views: 3719
Re: Do I need another objective?
On edit, I see you've addressed this, Zondar: What's not clear to me is if Snell's Law, ray tracing, and the Abbe diffraction limit entirely describe what happens when a specimen is illuminated and then attempts to illuminate the entire angle "seen" by an objective and described by its numerical ape...
- Thu Mar 28, 2024 10:04 pm
- Forum: Microscopes and optics
- Topic: Do I need another objective?
- Replies: 61
- Views: 3719
Re: Do I need another objective?
A dry (air-gapped) condenser will strictly limit the effective NA of any subsequent objective to a theoretical maximum of 1 (meaning somewhat less than 1 in practical terms). . . . As Hans noted above, it's likely a bit more complicated. The formula here (Thorlabs site) suggests an additive effect,...
- Thu Mar 28, 2024 9:28 pm
- Forum: My microscope
- Topic: Euromex Microscopes?
- Replies: 5
- Views: 818
Re: Euromex Microscopes?
. . . I collect microscopes and love to compare and contrast the various models both new and vintage from around the world :-) Thanks, Gary They're serviceable microscopes, but I wouldn't consider most of their models collectible—unless you were interested in the financial acquisition history of L ...
- Thu Mar 28, 2024 4:17 pm
- Forum: Beginner's corner
- Topic: Leica DMLB Condenser
- Replies: 10
- Views: 1052
Re: Leica DMLB Condenser
The slider coming in at an angle just under the DMLB lettering is for a DIC prism. The one for the analyzer is at about the same height, on the left side (facing the microscope).
- Wed Mar 27, 2024 9:47 pm
- Forum: Microscopes and optics
- Topic: Do I need another objective?
- Replies: 61
- Views: 3719
Re: Do I need another objective?
Given that you're shooting at higher magnifications, you're probably OK. I was imagining all those extra and fat pixels. But as you say; the extra or larger pixels from one of the newer mirrorless cameras won't help much at 400X and above. I have heard of vibration complaints from some Canon EOS use...
- Tue Mar 26, 2024 9:47 pm
- Forum: Microscopes and optics
- Topic: Do I need another objective?
- Replies: 61
- Views: 3719
Re: Do I need another objective?
Harry, The 60x Plan Apo oil 1.40 objective is wonderful for thin, often stained, and stationary specimens. While lower magnification than the 100x Plan Apo oil, it has the same theoretical resolution and (if memory serves) can peer a tiny fraction (something like .02mm is typical) further below a co...
- Tue Mar 26, 2024 9:22 pm
- Forum: Beginner's corner
- Topic: Leica DMLB Condenser
- Replies: 10
- Views: 1052
Re: Leica DMLB Condenser
For polarization, I suggest buying a decent-quality (so it goes to near full extinction) linear camera polarizer with a diameter that matches your field lens. It doesn't have to fit in or on the condenser. Should be under $20 or so on eBay. You'll also need a polarization slider to fit from the side...
- Tue Mar 26, 2024 4:14 am
- Forum: For forum members who want to buy and sell equipment
- Topic: For trade: microscope (DIC etc.) scopes & optics
- Replies: 39
- Views: 24121
Re: For trade: microscope (DIC etc.) scopes & optics
The first post has been updated once again. I'm now hoping to find - for trade or sale:
- UCL phase contrast condenser for Leica DMLS/DMLB
- Leica 2S1 (Ph2) phase ring
- Nikon 50/55i stage
- Nikon Eclipse PA20 DIC prism
- UCL phase contrast condenser for Leica DMLS/DMLB
- Leica 2S1 (Ph2) phase ring
- Nikon 50/55i stage
- Nikon Eclipse PA20 DIC prism
- Mon Mar 25, 2024 7:27 pm
- Forum: Microscopes and optics
- Topic: Substituting LED bulb for incandescent bulb
- Replies: 7
- Views: 792
Re: Substituting LED bulb for incandescent bulb
I've used the LED Edison-base tiny bulb replacements in a few scopes that needed them. The pluses are low cost, less heat, and longer life. However, while adequate, they don't seem much brighter. You do get the higher color temperature.
- Mon Mar 25, 2024 2:26 am
- Forum: Microscopes and optics
- Topic: Do I need another objective?
- Replies: 61
- Views: 3719
Re: Do I need another objective?
Thanks, Phil.