I'm only Nikon, so here's a list of Nikon scopes that match your criteria.
1.S-Ke (L-Ke)
2.Apophot
3.Biophot
4.Microphot (SA,FX,FXA)
5.E600,E800,E1000
Search found 137 matches
- Thu Aug 06, 2020 10:26 pm
- Forum: Microscopes and optics
- Topic: Illumination without diffusive elements
- Replies: 17
- Views: 7332
- Sun Jul 26, 2020 8:05 pm
- Forum: Specimens, samples and slides
- Topic: Staining a Chironomid Larva
- Replies: 2
- Views: 2093
Re: Staining a Chironomid Larva
Not aware of staining that would work for whole mounts... maybe silver staining could work since it's used for fairly thick neural tissue. If it could be sectioned, H&E would be the preference. If you have access to a fluorescent microscope, or a confocal/light sheet/MP microscope, fluorescent stain...
- Sun Jul 26, 2020 7:40 pm
- Forum: Specimens, samples and slides
- Topic: Picric acid substitute in Johansen's Safranin-Fast Green Protocol
- Replies: 5
- Views: 3259
Re: Picric acid substitute in Johansen's Safranin-Fast Green Protocol
I've never done plant/fungi specimens, but used Safranin-O and fast green FCF for staining cartilage sections fixed in PFA. If it's about the Safranin-O staining intensity, changing the solution pH (I used sodium citrate buffer) would improve things. Higher pH would yield more intense staining, but ...
- Tue Jun 30, 2020 1:52 pm
- Forum: For forum members who want to buy and sell equipment
- Topic: Nikon Microphot DIC components
- Replies: 6
- Views: 3388
Re: Nikon Microphot DIC components
Yes, Pete's comments already nails most of what is needed to be said. No, that kit won't fit the Microphot SA. The nosepiece holding the upper prism and analyzer will only fit the Optiphot-2 and some very late original Optiphots. However, the prisms and condenser are common components for the Microp...
- Tue Jun 30, 2020 5:40 am
- Forum: For forum members who want to buy and sell equipment
- Topic: Nikon Microphot DIC components
- Replies: 6
- Views: 3388
Re: Nikon Microphot DIC components
Umm, that DIC system photo actually belongs to me. I've granted use for a person who I won't name here, but I haven't given up copyrights for it.
Cheers,
John
Cheers,
John
- Mon Jun 15, 2020 7:06 am
- Forum: Illumination Techniques
- Topic: Phase turret question
- Replies: 1
- Views: 1728
Re: Phase turret question
Most phase turret condensers have mediocre NAs compared to other high-end condensers. Firstly due to lack of need to illuminate the absolute periphery of the available NA (most phase plates are only at the middle range of NA), and secondly to have longer working distances (especially the one you men...
- Sat May 30, 2020 5:56 am
- Forum: Beginner's corner
- Topic: LOMO 2.5 Objective Focusing
- Replies: 49
- Views: 14190
Re: LOMO 2.5 Objective Focusing
Not sure with LOMO, but some manufacturers have different parfocal distances for low power objectives, especially for those under 4x.
Cheers,
John
Cheers,
John
- Thu May 28, 2020 12:35 am
- Forum: Microscopes and optics
- Topic: GRIN lenses
- Replies: 2
- Views: 1321
Re: GRIN lenses
Perhaps cost?
Another would probably be field curvature that cant be compensated with the lens itself.
Cheers,
John
Another would probably be field curvature that cant be compensated with the lens itself.
Cheers,
John
- Thu May 28, 2020 12:03 am
- Forum: Beginner's corner
- Topic: Help! Questions about magnification and the eyepiece.
- Replies: 4
- Views: 2140
Re: Help! Questions about magnification and the eyepiece.
How large is the sensor? If sensor size is available, just divide it by the number of pixels on either axis and you'll get the approximate actual size of each pixel. Next imaging a stage micrometer will get you the total system magnification. A microscope camera should have its pixel size in its spe...
- Wed May 27, 2020 11:19 pm
- Forum: Microscopes and optics
- Topic: Interference Plane
- Replies: 21
- Views: 6685
Re: Interference Plane
Erm, wouldn't Thorlabs confocal system be a laser scanning confocal?? That's different from a spinning disk confocal.
Ah! Do you have a DM IL??
That slider slot under the head accepting the Hoffman sliders is actually conjugate with the objective BFP...
Cheers,
John
Ah! Do you have a DM IL??
That slider slot under the head accepting the Hoffman sliders is actually conjugate with the objective BFP...
Cheers,
John
- Wed May 27, 2020 6:47 am
- Forum: Microscopes and optics
- Topic: Interference Plane
- Replies: 21
- Views: 6685
Re: Interference Plane
Well... in a infinite system with a spinning disk confocal setup you should have the pinhole disk "after" the tube lens (or secondary objective, in Nikon jargon) That pinhole at the primary image plane eliminates light from the other defocused planes and at the same time acts as the illuminating ape...
- Wed May 27, 2020 3:41 am
- Forum: Microscopes and optics
- Topic: Interference Plane
- Replies: 21
- Views: 6685
Re: Interference Plane
BTW, Nikon does it like this in their "External Phase Contrast" system on their inverted microscopes.
- Wed May 27, 2020 3:26 am
- Forum: Microscopes and optics
- Topic: Interference Plane
- Replies: 21
- Views: 6685
Re: Interference Plane
The BFP is the Fourier plane of the objective :-) So if the BFP is at the left of L1, it will be relayed to the right of L2. The 4f relay is normally used to Fourier transform the "image" made by the objective by L1, and do a following inverse Fourier transform with L2. If you're relaying the BFP wi...
- Wed May 27, 2020 3:16 am
- Forum: Microscopes and optics
- Topic: Interference Plane
- Replies: 21
- Views: 6685
Re: Interference Plane
Yes, that 1987 patent would be a valid description of how the U-CPA works. And that 4f relay in the Bertrand position will kind of work... At least the Fourier plane wouldn't be at the center of L1 and L2. If the focal plane f1 to the left of L1 conincides with the objective BFP, the BFP will be rei...
- Wed May 27, 2020 1:29 am
- Forum: Microscopes and optics
- Topic: Interference Plane
- Replies: 21
- Views: 6685
Re: Interference Plane
>Pete
All manufacturers should publish that BFP location data! Olympus does and as you mention Leitz does... but Nikon doesn't disclose that data (at least on the www.)
All manufacturers should publish that BFP location data! Olympus does and as you mention Leitz does... but Nikon doesn't disclose that data (at least on the www.)
- Wed May 27, 2020 1:25 am
- Forum: Microscopes and optics
- Topic: Interference Plane
- Replies: 21
- Views: 6685
Re: Interference Plane
Yup, the paper from the Zia lab (Fig.1c) illustrates how a 4f-relay is implemented. Fig.1a has a Bertrand lens behind the tube lens, in this case consider the objective+tube lens as a unit functioning as a finite objective. Looking at it in that light will fit in with the 1966 patent by Leitz you sh...
- Tue May 26, 2020 10:45 pm
- Forum: Microscopes and optics
- Topic: Interference Plane
- Replies: 21
- Views: 6685
Re: Interference Plane
One needs to know where the conjugate planes reside. In your case assuming Koehler illumination is set up, conjugate planes would be like this; light source>condenser iris (condenser side prism interference plane)> objective exit pupil (objective back focal plane, objective side prism interference p...
- Sun Apr 26, 2020 1:51 am
- Forum: Microscopes and optics
- Topic: A permanent grease for sealed microscope components: Krytox
- Replies: 14
- Views: 7408
Re: A permanent grease for sealed microscope components: Krytox
Excellent stuff! I just got a 2oz tube of 206, and
it works wonders. I've went as far as relubing most of my objectives with correction collars, no sticking again! (hopefully)
BR,
John
it works wonders. I've went as far as relubing most of my objectives with correction collars, no sticking again! (hopefully)
BR,
John
- Mon Apr 13, 2020 3:01 pm
- Forum: Illumination Techniques
- Topic: Epi DIC woes
- Replies: 100
- Views: 53352
Re: Epi DIC woes
Are the prisms matched to the objective?
Shear amount should be tuned to be a tad under the objective resolution limit.
BR,
John
Shear amount should be tuned to be a tad under the objective resolution limit.
BR,
John
- Mon Apr 06, 2020 4:49 am
- Forum: Microscopes and optics
- Topic: DIC Prism Design
- Replies: 18
- Views: 9126
Re: DIC Prism Design
Excellent reference, the original document by Nomarski is rather cryptic (my French is horrible.) Kusaka describes the essence of prism design in the patent. One thing worth noting is Fig.7A-8. The shear amount is physically dictated by the wedge angle alpha (as in Fig.3), and the focal length of th...
- Wed Feb 05, 2020 8:44 am
- Forum: Microscopes and optics
- Topic: Making my own Leitz Heine oil cap - questions about adhesives and optics
- Replies: 29
- Views: 16112
Re: Making my own Leitz Heine oil cap - questions about adhesives and optics
With these kind of hemispheric top lenses, you have to consider "how thick" your slide glass will be. Note the tight tolerances for oil DF condensers.
BR,
John
BR,
John
- Mon Jan 27, 2020 2:30 am
- Forum: Illumination Techniques
- Topic: Making DIC Prisms
- Replies: 15
- Views: 10756
Re: Making DIC Prisms
Viktor, thank you for the Paolo Pozzi reference. I had some difficulty in determining objective BFPs on my bench so this method is excellent.
BR,
John
BR,
John
- Sun Jan 19, 2020 7:37 am
- Forum: Beginner's corner
- Topic: Nikon Optiphot Fluorescence illumination - BF?
- Replies: 15
- Views: 7084
Re: Nikon Optiphot Fluorescence illumination - BF?
Some corrections and follow ups. Finite CF metallurgical objectives (M Plans) by Nikon are tube length(TL) 210mm. TL160mm pre-CF metallurgical objectives exist, but they are the short barreled variety and will have parfocality problems. Regarding the mounting thread, M Plans are RMS so they will mou...
- Fri Dec 27, 2019 5:13 am
- Forum: Microscopes and optics
- Topic: Objective FN versus Ocular FN - Compound Microscope
- Replies: 11
- Views: 4520
Re: Objective FN versus Ocular FN - Compund Microscope
The field iris should be set to the smallest FN in the system. If you use a FN22 ocular on a FN26.5 head, it would be FN22. Many heads (and objectives) will allow fields up to nearly 30mm only limited by the ocular tube diameter themselves but whether that periphery would be pretty is a different ma...
- Sun Nov 24, 2019 12:58 am
- Forum: Beginner's corner
- Topic: Nikon Optiphot replacement bulb
- Replies: 9
- Views: 5303
Re: Nikon Optiphot replacement bulb
You’ll need a vertical facing filament to fill the aperture at high numerical apertures.
Cheers,
John
Cheers,
John
- Tue Sep 10, 2019 2:00 am
- Forum: Microscopes and optics
- Topic: Help for dismantling Leitz objective
- Replies: 4
- Views: 2695
Re: Help for dismantling Leitz objective
That objective has a safety spring nose, so it's essentially a lens holder inside a outer tube pressed against a forward flange with a spring. You have to access the inner lens assembly from the rear (mount side) loosening the ring screws. Though you'll eventually get inside to the elements you susp...
- Tue Sep 10, 2019 1:51 am
- Forum: Microscopes and optics
- Topic: Optiphot nose piece question
- Replies: 13
- Views: 6044
Re: Optiphot nose piece question
That reverse revolver adapter is a late addition to the original Optiphot. Though the revolver is reversed, there's no supplemental lenses (neither in the revolver, nor the stand limb) in the Optiphot2,and Labophot2 due to a redesigned thinner limb compared to the original Optiphot. Thus no addition...
- Mon Jul 15, 2019 2:23 am
- Forum: Microscopes and optics
- Topic: How to make a lambda wave plate?
- Replies: 23
- Views: 14546
Re: How to make a lambda wave plate?
Try mica. It used to be the material of choice
Cheers,
John
Cheers,
John
- Tue Jul 02, 2019 1:10 am
- Forum: Beginner's corner
- Topic: Darkfield - is resolution lost?
- Replies: 62
- Views: 23192
Re: Darkfield - is resolution lost?
Darkfield is inherently lower resolution than brightfield, and also is not a faithful representation of the specimen image, due to the loss of the zero-order light in the fourier-reconstructed (via interference) final image. Carl Partly agree, partly not. Please correct me if I'm not understanding ...
- Thu Jun 20, 2019 1:58 am
- Forum: Camera systems and imaging
- Topic: What are higher-magnification projective eyepieces good for?
- Replies: 29
- Views: 19513
Re: What are higher-magnification projective eyepieces good for?
"Well, why not get a 10x rather than a puny 2.5x when you can!"
I think that's the rationale behind it, and many would eventually come down to lower mags when they realize what resolution is
Cheers,
John
I think that's the rationale behind it, and many would eventually come down to lower mags when they realize what resolution is
Cheers,
John