Search found 52 matches

by abednego1995
Tue Sep 10, 2019 2:00 am
Forum: Microscopes and optics
Topic: Help for dismantling Leitz objective
Replies: 4
Views: 459

Re: Help for dismantling Leitz objective

That objective has a safety spring nose, so it's essentially a lens holder inside a outer tube pressed against a forward flange with a spring. You have to access the inner lens assembly from the rear (mount side) loosening the ring screws. Though you'll eventually get inside to the elements you susp...
by abednego1995
Tue Sep 10, 2019 1:51 am
Forum: Microscopes and optics
Topic: Optiphot nose piece question
Replies: 10
Views: 783

Re: Optiphot nose piece question

That reverse revolver adapter is a late addition to the original Optiphot. Though the revolver is reversed, there's no supplemental lenses (neither in the revolver, nor the stand limb) in the Optiphot2,and Labophot2 due to a redesigned thinner limb compared to the original Optiphot. Thus no addition...
by abednego1995
Mon Jul 15, 2019 2:23 am
Forum: Microscopes and optics
Topic: How to make a lambda wave plate?
Replies: 16
Views: 1228

Re: How to make a lambda wave plate?

Try mica. It used to be the material of choice :-)

Cheers,
John
by abednego1995
Tue Jul 02, 2019 1:10 am
Forum: Beginner's corner
Topic: Darkfield - is resolution lost?
Replies: 62
Views: 4333

Re: Darkfield - is resolution lost?

Darkfield is inherently lower resolution than brightfield, and also is not a faithful representation of the specimen image, due to the loss of the zero-order light in the fourier-reconstructed (via interference) final image. Carl Partly agree, partly not. Please correct me if I'm not understanding ...
by abednego1995
Thu Jun 20, 2019 1:58 am
Forum: Camera systems and imaging
Topic: What are higher-magnification projective eyepieces good for?
Replies: 18
Views: 3676

Re: What are higher-magnification projective eyepieces good for?

"Well, why not get a 10x rather than a puny 2.5x when you can!"

I think that's the rationale behind it, and many would eventually come down to lower mags when they realize what resolution is :-)

Cheers,
John
by abednego1995
Sat Jun 15, 2019 9:01 am
Forum: Microscopes and optics
Topic: Focusing by Changing the Tube Length
Replies: 6
Views: 744

Re: Focusing by Changing the Tube Length

That's exactly how modern inverted microscopes work.
Just shift the revolver plus objectives in relation to the specimen.

As long as the objective exit pupil is within accepting range of the tube lens entrance pupil, the separation between them does nothing with magnification.

Cheers,
John
by abednego1995
Wed Jun 12, 2019 10:43 am
Forum: Illumination Techniques
Topic: dIY DIC
Replies: 29
Views: 3299

Re: dIY DIC

Yup, Viktor, I think your getting that effect in your beforementioned setup. As you mention, in it's most primitive form DIC employs a pinhole condenser. PlasDIC improves on it by using a rectangular slit allowing somewhat higher NA information to pass through (though direction limited). So little i...
by abednego1995
Wed Jun 12, 2019 6:35 am
Forum: Illumination Techniques
Topic: dIY DIC
Replies: 29
Views: 3299

Re: dIY DIC

I had a bit of an email conversation with that seller regarding another plasDIC system he was selling. It , like the older Leitz in this link was a retrofit and a kind of crude one at that. There was a sticker that said DIC on the slider and it was cockeyed. There was no polarizer. He didn't answer...
by abednego1995
Wed Jun 05, 2019 12:21 am
Forum: Microscopy accessories
Topic: Need help, please, identifying accessories.
Replies: 12
Views: 1416

Re: Need help, please, identifying accessories.

The first Leitz unit is a incident illumination unit for the Ultropak. Without the objectives I'm not sure how much it will fetch, but the illuminator itself is quite rare. Nice find.
by abednego1995
Tue May 14, 2019 3:43 am
Forum: Microscopes and optics
Topic: MitutoyoBD Objective Threads
Replies: 3
Views: 1558

Re: MitutoyoBD Objective Threads

Wow, that's great precision there! Wonder if it's sturdy enough to support the heavy BD's when used as an adapter.
by abednego1995
Mon May 13, 2019 1:17 am
Forum: Microscopes and optics
Topic: WYKO Mrau Interferometer Objective
Replies: 1
Views: 750

Re: WYKO Mrau Interferometer Objective

That's interesting, why not use monochromatic light? That would greatly increase band-band contrast, and moreover if using a narrow band filter or source you can easily determine height difference with the wavelength.

Cheers,
John
by abednego1995
Sat May 11, 2019 4:41 am
Forum: Beginner's corner
Topic: Inverted vs. Upright Microscopes
Replies: 26
Views: 4024

Re: Inverted vs. Upright Microscopes

I have successfully drilled 3-5mm thick glass plates without breaking, so, not a piece of cake but not a horrible challenge either. Not that I recommend it, if there is an alternative. Actually , the challenge is not so much the cutting, but the glueing of a coverslip to the slide . I mean, creatin...
by abednego1995
Fri May 10, 2019 3:15 pm
Forum: Beginner's corner
Topic: Inverted vs. Upright Microscopes
Replies: 26
Views: 4024

Re: Inverted vs. Upright Microscopes

Drilling holes in glass slides sounds like a piece of cake, but in reality,
you realize it's quite hard after a dozen shattered slides :roll: (even underwater with those coring bits.)

Wish someone near has a water jet cutter...
by abednego1995
Sun May 05, 2019 4:12 pm
Forum: Beginner's corner
Topic: Inverted vs. Upright Microscopes
Replies: 26
Views: 4024

Re: Inverted vs. Upright Microscopes

Yup, I absolutely agree with getting glass bottomed petri dishes for normal benchtop inverted microscopes. You can't get any better. However, even the smallest 35mm diameter dishes (are there smaller ones?) it would be very difficult to manipulate on the Swift stage (It's very unforgiving due to its...
by abednego1995
Sun May 05, 2019 2:51 pm
Forum: Beginner's corner
Topic: Inverted vs. Upright Microscopes
Replies: 26
Views: 4024

Re: Inverted vs. Upright Microscopes

Nikon used to sell them for their Model H microscopes, but it seems to have been an obscure item since I've never seen one in real life.
Any adhesive would do. I've used silicone, epoxy, even Entellan, but all work OK.

Cheers,
John
by abednego1995
Sun May 05, 2019 8:59 am
Forum: Beginner's corner
Topic: Inverted vs. Upright Microscopes
Replies: 26
Views: 4024

Re: Inverted vs. Upright Microscopes

Hi! Those swift microscopes are nice, great catch. If you need to observe wet mounts on a regular basis, making well slides from acrylic or glass with holes around 10-12mm diameter in the middle with coverslips bonded to the bottom are a nice investment. Having a few for a trip cuts all the hassle i...
by abednego1995
Sat Apr 27, 2019 9:37 am
Forum: Camera systems and imaging
Topic: NIKON SPOT METER
Replies: 7
Views: 1527

Re: NIKON SPOT METER

It's already mentioned in Micheal's link. The N-1 is for the Multiphot.
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Cheers,
John
by abednego1995
Sun Apr 21, 2019 9:46 am
Forum: Illumination Techniques
Topic: EPI dedicated hybrid
Replies: 9
Views: 3081

Re: EPI dedicated hybrid

Hi George! Nice setup, but I'm curious on how you managed to get TL160mm on it.

Cheers,
John
by abednego1995
Fri Apr 19, 2019 4:12 am
Forum: For forum members who want to buy and sell equipment
Topic: Looking For Nikon S-series Trinocular or Binocular head piece.
Replies: 3
Views: 3216

Re: Looking For Nikon S-series Trinocular or Binocular head piece.

Hi, that's a nasty crack in there... Having double images indicates a mismatch in the optical axis somewhere in the train. In the Nikon S series Jentsch type binocular, this often happens with the displacement of the center columnar prism in your second photo. When you disassemble this head, touchin...
by abednego1995
Mon Apr 15, 2019 2:55 am
Forum: Microscopes and optics
Topic: Double images
Replies: 18
Views: 5669

Re: Double images

If it persists between different scopes, could it be a case of strabismus?
by abednego1995
Sun Apr 14, 2019 3:31 am
Forum: Microscopes and optics
Topic: Nikon S-Ke with lots of accessories - A humbling experience, help needed
Replies: 24
Views: 5947

Re: Nikon S-Ke with lots of accessories - A humbling experience, help needed

Hi, great haul you have there! Lots of questions, and some are already answered well in the thread. I'll add in where there is improvement possible. Q1: Can you get new lamps nowadays? A1: Yes, Nikon actually still lists them as current items. IMG_1973.JPG The left bulb is the 6V30W bulb for the bas...
by abednego1995
Tue Apr 09, 2019 4:21 am
Forum: Microscopes and optics
Topic: Need advice with DIC prism
Replies: 10
Views: 1467

Re: Need advice with DIC prism

The C-CU Universal Condenser was meant for the early E800,600 series. It has 2 interchangeable condenser tops, Dry 0.9 and Oil 1.4 (No water immersion top available for C-CU.) (Yes there is a water immersion top for the T-CT-E condenser, but it will not fit upright microscopes) It doesn't matter at ...
by abednego1995
Thu Mar 14, 2019 11:15 pm
Forum: My microscope
Topic: NIKON Optiphot C-mount adapter
Replies: 5
Views: 2586

Re: NIKON Optiphot C-mount adapter

Ah, and I forgot to mention. That trinocular head is a quite rare type and it has an ENG (B4) mount under that black ring. This specific adapter will allow direct projection of the primary image sans relay optics with the benefit of a larger image circle than 16mm with the 1x relay. With a mag of 1x...
by abednego1995
Thu Mar 14, 2019 10:44 am
Forum: My microscope
Topic: NIKON Optiphot C-mount adapter
Replies: 5
Views: 2586

Re: NIKON Optiphot C-mount adapter

Congrat's on your aqcuirement! I see you have it set up for fluorescence, nice to have one. That's a 1x projection lens, and it will project a 16mm diameter image 115mm above from the lower shoulder of the lens. You'll need this adapter to hook up a C-mount camera. It's obsolete but comes up time to...
by abednego1995
Fri Mar 08, 2019 4:58 am
Forum: Microscopes and optics
Topic: 160/-, planaapo low mag. objectives...please advise?
Replies: 11
Views: 1442

Re: 160/-, planaapo low mag. objectives...please advise?

I totally agree. There would be specific design criteria for each objective, and that would mean different residual SAs for each. One thing I've yet to experiment myself is testing objectives at different wavelengths in respect to SA. I'm suspecting that if you use an objective in a wavelength other...
by abednego1995
Fri Mar 08, 2019 12:46 am
Forum: Microscopes and optics
Topic: 160/-, planaapo low mag. objectives...please advise?
Replies: 11
Views: 1442

Re: 160/-, planaapo low mag. objectives...please advise?

Well, as a rule of thumb if you're using objectives with NA lower than 0.4 it wouldn't matter much whether you have a coverslip or not. It applies for any objective. The catch with Hobbyst46's objectives, is that they are both immersion objectives. As long as the refractive index of the coverslip an...
by abednego1995
Fri Mar 08, 2019 12:22 am
Forum: Microscopes and optics
Topic: 100x NCG oil: what is it used for?
Replies: 5
Views: 1071

Re: 100x NCG oil: what is it used for?

Smears are fixed with methanol or heat, so if done "properly" it shouldn't pose as a biohazard.

P.S. Another story, but I would be careful with second-hand counting chamber slides since they use cells/blood in a raw state...
by abednego1995
Thu Feb 28, 2019 6:56 am
Forum: For forum members who want to buy and sell equipment
Topic: Looking for Nikon CF Plan/Phase Short Barrel Objectives (FOUND)
Replies: 8
Views: 1883

Re: Looking for Nikon CF Plan/Phase Short Barrel Objectives

Another small thing. (probably of the purist sort. and only a problem at extreme NA's above 1) Nikon short barrel objectives have a parfocal distance of 33.6mm, and later CF, NCF (DIN standard) objectives have 45mm. Both will admittedly fit on a S-Kt, however with the 45mm objectives at the expense ...
by abednego1995
Thu Feb 21, 2019 5:21 am
Forum: For forum members who want to buy and sell equipment
Topic: Where to buy a drop stop ring (?) for a pole-mounted microscope
Replies: 16
Views: 1869

Re: Where to buy a drop stop ring (?) for a pole-mounted microscope

Hi!
Not sure of the exact part for the WILD (It's Leica right now isn't it?)
But if you wouldn't mind aesthetics, a cheap and proven option I use is a photographic clamp (from Manfrotto I believe) for use on their poles.

Cheers,
John
by abednego1995
Thu Feb 14, 2019 8:42 am
Forum: Beginner's corner
Topic: What alternatives are there to match the eye piece field of view with a camera's field of view.
Replies: 5
Views: 2488

Re: What alternatives are there to match the eye piece field of view with a camera's field of view.

If you want to have a sense of the field that is imaged, getting an ocular with photo reticles as gastrotrichman says is the simplest way. However, those oculars and reticles are pretty rare and depending on your imaging setup the total system magnification might not match the reticle design. Making...