Search found 97 matches

by abednego1995
Thu Sep 03, 2020 5:05 am
Forum: Microscopy accessories
Topic: New infinity corrected water immersion 100X objectives soon.
Replies: 18
Views: 769

Re: New infinity corrected water immersion 100X objectives soon.

Very interesting, and awesome!
Are the objectives going to be corrected for 0.17mm coverslips or will they be just for dipping??

Cheers,
John
by abednego1995
Thu Aug 27, 2020 1:03 pm
Forum: Microscopes and optics
Topic: Opening up an objective!
Replies: 6
Views: 499

Re: Opening up an objective!

The horror! The horror!
by abednego1995
Wed Aug 26, 2020 12:00 am
Forum: Microscopes and optics
Topic: Nikon Plan Apo Lambda 20x - ok??
Replies: 39
Views: 1321

Re: Nikon Plan Apo Lambda 20x - ok??

Ah, Ok. I’ve found it anyway. Nikon writes different info on the front and back of the objective. The seller’s photo has it on the second photo. It’s a genuine PA Lambda 20x.

Cheers,
John
by abednego1995
Tue Aug 25, 2020 11:48 pm
Forum: Microscopes and optics
Topic: Nikon Plan Apo Lambda 20x - ok??
Replies: 39
Views: 1321

Re: Nikon Plan Apo Lambda 20x - ok??

BTW, do you have the URL?
Your description of the listing having Lambda etc.. only in the listing and not on the barrel...is somewhat "concerning"
by abednego1995
Tue Aug 25, 2020 11:38 pm
Forum: Microscopes and optics
Topic: Nikon Plan Apo Lambda 20x - ok??
Replies: 39
Views: 1321

Re: Nikon Plan Apo Lambda 20x - ok??

Well, I've seen the Lambda 20x in pathology slide scanners but that's a recent application, and I'd doubt it would come with a case. If there are multiple examples, I would suspect a (large) lab closure, or some kind of government surplus. In that case, the intent for procurement would likely be for...
by abednego1995
Tue Aug 25, 2020 11:04 pm
Forum: Microscopes and optics
Topic: Nikon Plan Apo Lambda 20x - ok??
Replies: 39
Views: 1321

Re: Nikon Plan Apo Lambda 20x - ok??

Good catch, but I'll add in some more info. The CFI60 Plan Apo Lambda 20x wasn't used in gene sequencers as far as I know. The latest machines use discrete optics tailored to each machines specific need (i.e. WD, compensation, wavelength characteristics). The brownish barrelled variants of the CFI60...
by abednego1995
Mon Aug 24, 2020 12:37 pm
Forum: Resources (online, books etc.)
Topic: Nikon Ske or other Nikon?
Replies: 5
Views: 349

Re: Nikon Ske or other Nikon?

Thanks, that makes it clearer. That's the earliest version of the S-Ke in production from 1963-65. As Pete points out, this particular version with separate knobs for coarse/fine focus doesn't suffer from nylon gear fracture that is endemic with the later models. However, from the photo the scope is...
by abednego1995
Mon Aug 24, 2020 7:35 am
Forum: Pictures and Videos
Topic: Stentor in pol interference contrast
Replies: 8
Views: 335

Re: Stentor in pol interference contrast

Brilliant movie!
Jamin-Lebedev interference?
by abednego1995
Sun Aug 23, 2020 10:52 pm
Forum: Resources (online, books etc.)
Topic: Nikon Ske or other Nikon?
Replies: 5
Views: 349

Re: Nikon Ske or other Nikon?

Looks like there's no image?
by abednego1995
Thu Aug 20, 2020 5:25 am
Forum: Microscopes and optics
Topic: Phase Telescope with reticle
Replies: 3
Views: 300

Re: Phase Telescope with reticle

Elaborating on Hans's post, assuming you have a phase telescope that is of Keplarian make, there is a virtual image of the objective back focal plane (BFP) to be observed just below the eyepiece. So if you can add a reticle at that plane, that will enable you to superimpose it on the BFP image. Just...
by abednego1995
Mon Aug 17, 2020 10:23 am
Forum: Camera systems and imaging
Topic: How important is axial exit/entrance pupil positioning in an afocal configuration?
Replies: 14
Views: 1017

Re: How important is axial exit/entrance pupil positioning in an afocal configuration?

Good place to start would be the equations available at Edmund Optics. It's originally for determining the acceptable displacement between an infinite objective and it's tube lens but it also would apply in an afocal setup. https://www.edmundoptics.com/knowledge-center/application-notes/microscopy/u...
by abednego1995
Fri Aug 14, 2020 1:57 pm
Forum: Microscopes and optics
Topic: NIKON OBJETIVO MICROSCOPIO APO 100X PLAN HI 1.30
Replies: 22
Views: 804

Re: NIKON OBJETIVO MICROSCOPIO APO 100X PLAN HI 1.30

Hmmmm.... how to prove if it uses fluorite...
No idea there, maybe dissasemble it? But destroying an otherwise fine objective is... painful.

Well, if the design calls for fluorite, why doubt it?
by abednego1995
Fri Aug 14, 2020 12:01 am
Forum: Microscopes and optics
Topic: NIKON OBJETIVO MICROSCOPIO APO 100X PLAN HI 1.30
Replies: 22
Views: 804

Re: NIKON OBJETIVO MICROSCOPIO APO 100X PLAN HI 1.30

Here's the diagram for the PlanApo 100x NA1.30 from a Japanese publication.
The hatched elements are probably fluorite.

Systematic Survey on Optical Microscope Technology in Japan, C.Nagano, 国立科学博物館技術の系統化調査報告 Vol.24 2017. March. p.373
by abednego1995
Thu Aug 13, 2020 11:56 pm
Forum: Microscopes and optics
Topic: NIKON OBJETIVO MICROSCOPIO APO 100X PLAN HI 1.30
Replies: 22
Views: 804

Re: NIKON OBJETIVO MICROSCOPIO APO 100X PLAN HI 1.30

The parfocal distance is only 33.6mm compared with the 45mm of the CF series objectives intended for your Optiphot. Did they go from 33.6mm to the JIS Standard 36mm and then to DIN 45mm or did they go straight to DIN? Well, I'm not quite sure where that number for the JIS came from since Nikon has ...
by abednego1995
Thu Aug 13, 2020 3:54 pm
Forum: Microscopes and optics
Topic: NIKON OBJETIVO MICROSCOPIO APO 100X PLAN HI 1.30
Replies: 22
Views: 804

Re: NIKON OBJETIVO MICROSCOPIO APO 100X PLAN HI 1.30

Well, it's a difficult problem there. Not techincally "lying" but it's a matter of where you place the design goals and acceptable tolerances. At the time it came out, it was probably comparable to competitors designs. But over time, it became "unacceptable" and I think it was Leitz (not sure here, ...
by abednego1995
Thu Aug 13, 2020 3:42 pm
Forum: Microscopes and optics
Topic: An interesting patent [for microscope objective]
Replies: 3
Views: 315

Re: An interesting patent [for microscope objective]

That's one expensive lens design...
They're using 2 fluorite elements in the first embodiment, and 3(!) in the second. If they had redesigned it in 1975 (That's when Schott came out with their first ED glass FK01), that would have made an well corrected and affordable LWD objective.
by abednego1995
Thu Aug 13, 2020 2:53 pm
Forum: Microscopes and optics
Topic: NIKON OBJETIVO MICROSCOPIO APO 100X PLAN HI 1.30
Replies: 22
Views: 804

Re: NIKON OBJETIVO MICROSCOPIO APO 100X PLAN HI 1.30

That's a Plan Apo 100x NA1.30 for the older "black" painted S series microscopes from the 60-70s. The parfocal distance is only 33.6mm compared with the 45mm of the CF series objectives intended for your Optiphot. Hence the trouble mounting it (it's impossible to raise the stage to compensate) Plus,...
by abednego1995
Sat Aug 08, 2020 9:55 am
Forum: Microscopes and optics
Topic: Illumination without diffusive elements
Replies: 16
Views: 1215

Re: Illumination without diffusive elements

>Hans Yes, nice microscope but I don't own one. Too large and cumbersome with too little fraternity:-) The Apophot has a dial which has settings for each magnification, so as long as the operator is keen to adjust it every time they change mags it works like a charm. But again it's up to the operato...
by abednego1995
Fri Aug 07, 2020 5:00 am
Forum: Microscopes and optics
Topic: Illumination without diffusive elements
Replies: 16
Views: 1215

Re: Illumination without diffusive elements

>Hans Good point. Though I listed the E series as "diffusorless" actual operation normally is favored with the diffusor in the light path in diascopic illumination. This is due to compromises in the design that doesn't allow for filament position adjustment. The filament image is too large at low NA...
by abednego1995
Thu Aug 06, 2020 10:26 pm
Forum: Microscopes and optics
Topic: Illumination without diffusive elements
Replies: 16
Views: 1215

Re: Illumination without diffusive elements

I'm only Nikon, so here's a list of Nikon scopes that match your criteria.

1.S-Ke (L-Ke)
2.Apophot
3.Biophot
4.Microphot (SA,FX,FXA)
5.E600,E800,E1000
by abednego1995
Sun Jul 26, 2020 8:05 pm
Forum: Specimens, samples and slides
Topic: Staining a Chironomid Larva
Replies: 2
Views: 293

Re: Staining a Chironomid Larva

Not aware of staining that would work for whole mounts... maybe silver staining could work since it's used for fairly thick neural tissue. If it could be sectioned, H&E would be the preference. If you have access to a fluorescent microscope, or a confocal/light sheet/MP microscope, fluorescent stain...
by abednego1995
Sun Jul 26, 2020 7:40 pm
Forum: Specimens, samples and slides
Topic: Picric acid substitute in Johansen's Safranin-Fast Green Protocol
Replies: 5
Views: 404

Re: Picric acid substitute in Johansen's Safranin-Fast Green Protocol

I've never done plant/fungi specimens, but used Safranin-O and fast green FCF for staining cartilage sections fixed in PFA. If it's about the Safranin-O staining intensity, changing the solution pH (I used sodium citrate buffer) would improve things. Higher pH would yield more intense staining, but ...
by abednego1995
Tue Jun 30, 2020 1:52 pm
Forum: For forum members who want to buy and sell equipment
Topic: Nikon Microphot DIC components
Replies: 6
Views: 801

Re: Nikon Microphot DIC components

Yes, Pete's comments already nails most of what is needed to be said. No, that kit won't fit the Microphot SA. The nosepiece holding the upper prism and analyzer will only fit the Optiphot-2 and some very late original Optiphots. However, the prisms and condenser are common components for the Microp...
by abednego1995
Tue Jun 30, 2020 5:40 am
Forum: For forum members who want to buy and sell equipment
Topic: Nikon Microphot DIC components
Replies: 6
Views: 801

Re: Nikon Microphot DIC components

Umm, that DIC system photo actually belongs to me. I've granted use for a person who I won't name here, but I haven't given up copyrights for it.

Cheers,
John
by abednego1995
Mon Jun 15, 2020 7:06 am
Forum: Illumination Techniques
Topic: Phase turret question
Replies: 1
Views: 508

Re: Phase turret question

Most phase turret condensers have mediocre NAs compared to other high-end condensers. Firstly due to lack of need to illuminate the absolute periphery of the available NA (most phase plates are only at the middle range of NA), and secondly to have longer working distances (especially the one you men...
by abednego1995
Sat May 30, 2020 5:56 am
Forum: Beginner's corner
Topic: LOMO 2.5 Objective Focusing
Replies: 49
Views: 2129

Re: LOMO 2.5 Objective Focusing

Not sure with LOMO, but some manufacturers have different parfocal distances for low power objectives, especially for those under 4x.

Cheers,
John
by abednego1995
Thu May 28, 2020 12:35 am
Forum: Microscopes and optics
Topic: GRIN lenses
Replies: 2
Views: 335

Re: GRIN lenses

Perhaps cost?
Another would probably be field curvature that cant be compensated with the lens itself.

Cheers,
John
by abednego1995
Thu May 28, 2020 12:03 am
Forum: Beginner's corner
Topic: Help! Questions about magnification and the eyepiece.
Replies: 4
Views: 424

Re: Help! Questions about magnification and the eyepiece.

How large is the sensor? If sensor size is available, just divide it by the number of pixels on either axis and you'll get the approximate actual size of each pixel. Next imaging a stage micrometer will get you the total system magnification. A microscope camera should have its pixel size in its spe...
by abednego1995
Wed May 27, 2020 11:19 pm
Forum: Microscopes and optics
Topic: Interference Plane
Replies: 21
Views: 1248

Re: Interference Plane

Erm, wouldn't Thorlabs confocal system be a laser scanning confocal?? That's different from a spinning disk confocal.

Ah! Do you have a DM IL??
That slider slot under the head accepting the Hoffman sliders is actually conjugate with the objective BFP...

Cheers,
John