Search found 1422 matches

by MicroBob
Tue Oct 22, 2019 2:43 pm
Forum: Specimens, samples and slides
Topic: Mountants for fluorescence microscopy
Replies: 4
Views: 50

Re: Mountants for fluorescence microscopy

From what I found about dammar and autofluorescence was that it should have some. My own test showed that it has some, but very very little at a layer of ca. 0.17mm thickness. I will try to get some Benzyl alcohol or dry the varnish and resolve in xylene. I hope that the different solvent doesn't ch...
by MicroBob
Tue Oct 22, 2019 2:34 pm
Forum: Beginner's corner
Topic: Am I doing something wrong?
Replies: 8
Views: 106

Re: Am I doing something wrong?

Hi Phil, thank you for pointing this out: The resolution is about 0,2µm, not 20µm! So the bacterium looks roughly as if seen an a 5x5 pixel matrix. Bob Bacteria are about 1µm in diameter. The resolution of a light microscope with oil immersion objective is about 20µm. This means that you can resolve...
by MicroBob
Tue Oct 22, 2019 8:34 am
Forum: Introduce yourself
Topic: Hi just starting in Microscopie Almere The Netherlands
Replies: 6
Views: 132

Re: Hi just starting in Microscopie Almere The Netherlands

Hi Bob, of your images just the one with the microscope is visible for me. Generally the eyepiece can't extract more out of the image than the objective has resolved, so it is usually best to use 10x eyepieces. If you can arrange with the current eyepiece, I would suggest to just use it and gain som...
by MicroBob
Tue Oct 22, 2019 8:21 am
Forum: Beginner's corner
Topic: Am I doing something wrong?
Replies: 8
Views: 106

Re: Am I doing something wrong?

Bacteria are about 1µm in diameter. The resolution of a light microscope with oil immersion objective is about 20µm. This means that you can resolve the bacterium and roughly see it's shape (. or - or ~) but not much more. To see it you have to adjust your condenser for maximum resolution - this is ...
by MicroBob
Tue Oct 22, 2019 8:09 am
Forum: Specimens, samples and slides
Topic: Mountants for fluorescence microscopy
Replies: 4
Views: 50

Re: Mountants for fluorescence microscopy

Hi Doron, I tested with the excitation filter combination that blocks above ca. 460nm. I included the Caedax-Aroclor mix as I have it and the r.i. would be not extremely high. I don't know the r.i. of this mixture and I don't know the typ and concentration of the Aroclor. It probably was mixed as a ...
by MicroBob
Mon Oct 21, 2019 7:08 pm
Forum: Specimens, samples and slides
Topic: Mountants for fluorescence microscopy
Replies: 4
Views: 50

Mountants for fluorescence microscopy

Hi together, I've just started to experiment with Epi fluorescence microscopy: http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=6&t=8206&sid=235a0e88a4cf3c5270dde54d8ecc7191 One thing I became aware of immediately was that my preferred mountant for botanical slides, Euparal, shows a lot...
by MicroBob
Sun Oct 20, 2019 8:17 pm
Forum: Introduce yourself
Topic: Hi just starting in Microscopie Almere The Netherlands
Replies: 6
Views: 132

Re: Hi just starting in Microscopie Almere The Netherlands

Hi, welcome to the forum! Eyepieces with "glasses" symbol are for people who use the microscope with glasses on. I wear glasses, but take them off when I look through the microscope. The eye relief can be better on a new eyepiece, don't expect much other improvements. Woukd you like to show a pictur...
by MicroBob
Sun Oct 20, 2019 7:56 pm
Forum: Pictures and Videos
Topic: Sequoia gigantensis cross section in Epi-Fluorescence
Replies: 6
Views: 106

Re: Sequoia gigantensis leaf cross section in Epi-Fluorescence

Hi Doron, one of my sons is often picking up plants or makes cuttlings and grows plants from them. This usually works quite well, better than with the Sequoia cuttlings. There are som specific tricks like applying willow bark solution (a tree that grows in very wet spots, e.g. next to river beds) an...
by MicroBob
Sun Oct 20, 2019 6:32 pm
Forum: Pictures and Videos
Topic: Sequoia gigantensis cross section in Epi-Fluorescence
Replies: 6
Views: 106

Re: Sequoia gigantensis cross section in Epi-Fluorescence

I did some more testing and optimized my lighting unit.
The next stain I tested was rhodamin B in combination with acriflavin.
by MicroBob
Sun Oct 20, 2019 1:10 pm
Forum: Pictures and Videos
Topic: Sequoia gigantensis cross section in Epi-Fluorescence
Replies: 6
Views: 106

Re: Sequoia gigantensis cross section in Epi-Fluorescence

They live quite happily in Europe and it is possible to grow them from seeds but it is not easy. Our experiments with cuttlings and seeds didn't fruit. Others have managed to grow plants, there are some tricks to optimize the results, as always.
by MicroBob
Sun Oct 20, 2019 11:43 am
Forum: Pictures and Videos
Topic: Sequoia gigantensis cross section in Epi-Fluorescence
Replies: 6
Views: 106

Sequoia gigantensis cross section in Epi-Fluorescence

Hi together, In the 1860s Kaiser Wilhelm ordered a pound of Sequoia gigantensis seeds, fitting to his general mind set. He didn't know that these big trees have tiny seeds and he got a lot of them. They were distributed to different forrest wardens and plants were grown from them. From this time we ...
by MicroBob
Sat Oct 19, 2019 8:31 pm
Forum: Illumination Techniques
Topic: Incident fluorescence: Ficus leaf tip cross section
Replies: 20
Views: 688

Re: Incident fluorescence: Ficus leaf tip cross section

I repeated the test with the excitation filters: The B 46 filter alone gave a medium green background, so this light passes the dichroic mirror and the Y 52 emission filter. When I add the 47/63 as a second excitation filter, the background becomes nearly black. Not logical but repeatable. My idea i...
by MicroBob
Sat Oct 19, 2019 2:58 pm
Forum: Microscopes and optics
Topic: First Surface Mirror?
Replies: 13
Views: 1754

Re: First Surface Mirror?

What would be a good test for a mirror? Looking into it with a pair of binoculars at a subject with fine detail?
Ordinary sheet metal is not that flat so it is the question whether they ground it flat or not.
by MicroBob
Sat Oct 19, 2019 2:15 pm
Forum: Illumination Techniques
Topic: Incident fluorescence: Ficus leaf tip cross section
Replies: 20
Views: 688

Re: Incident fluorescence: Ficus leaf tip cross section

I got the filters and changed the setup: Excitation: Jenmed Dichrolight M 47/63 plus B46 ( the B46 alone led to a green background!) Dichroitic Mirror Zeiss FT 510 Blocking filter Jenmed Dichrolight Y 52 Object: Leaf of small (12cm) coniferous plant Super quick hand section with cutter blade Objecti...
by MicroBob
Sat Oct 19, 2019 12:54 pm
Forum: Specimens, samples and slides
Topic: Disposing of stains from home microscopy lab
Replies: 8
Views: 121

Re: Disposing of stains from home microscopy lab

Hi Mike, It's a good idea to think about this before producing the waste. In my view proper waste disposal is the main reason to limit the use of chemicals in the home lab. Microscopy recipes have been developed without much concern for environmental issues so it is a good idea to think about what i...
by MicroBob
Sat Oct 19, 2019 8:49 am
Forum: Illumination Techniques
Topic: Incident fluorescence: Ficus leaf tip cross section
Replies: 20
Views: 688

Re: Incident fluorescence: Ficus leaf tip cross section

Thank you for your explanations, Doron! First: I got the filters of the first filter set mixed up (in writing). These are the specifications of my two filter sets: Filtersatz 14 488014-0000-000 BP 510-560 FT 580 LP 590 Filtersatz 17 488017-9901-000 BP 485/20 FT 510 BP 515-565 So the order of excitat...
by MicroBob
Fri Oct 18, 2019 8:45 pm
Forum: Illumination Techniques
Topic: Incident fluorescence: Ficus leaf tip cross section
Replies: 20
Views: 688

Re: Incident fluorescence: Ficus leaf tip cross section

The big microscope makers offer nice listings of filters and dyes as they like to sell their expensive stuff. For Rhodamin Zeiss has quite e few entries: https://www.micro-shop.zeiss.com/de/de/shop/filterAssistant/dyes/ If I haven't mixed this up in my short test this was a Wacker stain with Rhodami...
by MicroBob
Fri Oct 18, 2019 8:26 pm
Forum: Microscopes and optics
Topic: First Surface Mirror?
Replies: 13
Views: 1754

Re: First Surface Mirror?

Hi Doron, what are the dental mirrors made of? Aluminium? If precise size is important the hard discs I used are not great. The diamond wheel didn't cut them well (maybe a question of the right wheel) and the mirror got hot which induces cracks. With patience and water cooling it will be possible to...
by MicroBob
Fri Oct 18, 2019 7:57 pm
Forum: Microscopes and optics
Topic: First Surface Mirror?
Replies: 13
Views: 1754

Re: First Surface Mirror?

Well, I just took one and used it for my fluorescence condenser side lighting unit. The surface looks perfectly silvered to me, I didn't find a hint to uneven spectral reflection. This was out of my stack of optic DIY material, a 2,5'' disc. It was made from some kind of glass. I tried to cut it wit...
by MicroBob
Fri Oct 18, 2019 7:43 pm
Forum: Illumination Techniques
Topic: Incident fluorescence: Ficus leaf tip cross section
Replies: 20
Views: 688

Re: Incident fluorescence: Ficus leaf tip cross section

Hi together, I have built an LED lighting unit that shines from the side over a mirror and into the back of the reflected light fluorescence condenser III RS. This was quite difficult as the gap is just 24mm wide and I wanted to attatch the lighting unit to the stand, not the condenser. It works qui...
by MicroBob
Thu Oct 17, 2019 7:13 pm
Forum: Pictures and Videos
Topic: Podocystis sp.
Replies: 4
Views: 53

Re: Podocystis sp.

Hi Glen,
beautiful image and lots of detail in this small specimen! Would you say it is in fully living condition?

Bob
by MicroBob
Thu Oct 17, 2019 8:11 am
Forum: My microscope
Topic: Euromex MB 1051, Canon m6 (projection)
Replies: 7
Views: 142

Re: Euromex MB 1051, Canon m6 (projection)

The 22mm camera lens leads to the circular image on the sensor with a lot of black around it. This is no big problem but you don't use all camera resolution that is available and have always to do a cutout to use a picture. In video mode some cameras use only a smaller inner part of the sensor image...
by MicroBob
Wed Oct 16, 2019 8:51 am
Forum: Microscopes and optics
Topic: Field diaphragm problem
Replies: 21
Views: 597

Re: Field diaphragm problem

Hi together, this has developed into a quite interesting thread! At first a riddle, then funny (the mirror that was stuck back in reverse orientation) and then very informative with angled filter seats and interference filters for light correction. I know and use practically only microscopes of the ...
by MicroBob
Tue Oct 15, 2019 10:18 am
Forum: Introduce yourself
Topic: Hello from northern spain
Replies: 2
Views: 76

Re: Hello from northern spain

Hi Martin, welcome to the forum!
I'm sure you will find here people who share your interests.

Bob
by MicroBob
Tue Oct 15, 2019 4:43 am
Forum: Illumination Techniques
Topic: Diverse views of Pleurosigma on a strew slide
Replies: 8
Views: 318

Re: Diverse views of Pleurosigma on a strew slide

Hi Doron,
I would have expected the oblique image to show more detail. I have made it a bit brighter, bit more contrasty and sharpened abit. Sometimes such editing leads to a gritty look and enhances the remaining dirt but in this case I like the result.

Bob
by MicroBob
Mon Oct 14, 2019 12:01 pm
Forum: Identification help
Topic: worm
Replies: 4
Views: 122

Re: worm

Hi Timur,
Nice find and a good video! I have never seen this species before.

Bob
by MicroBob
Sun Oct 13, 2019 11:08 pm
Forum: Beginner's corner
Topic: Fusing glass slides
Replies: 23
Views: 840

Re: Fusing glass slides

One point to consider would be what happens in case of spillage. Flamable and aggressive solvents in an expensive microscope - not good. Is there space for a second flat safety container?
by MicroBob
Sun Oct 13, 2019 10:35 pm
Forum: Illumination Techniques
Topic: Incident fluorescence: Ficus leaf tip cross section
Replies: 20
Views: 688

Re: Incident fluorescence: Ficus leaf tip cross section

Hi Doron,
thank you for this hint, it is obvious but I had overlooked it completely. I think I will go for a black cover for the microscope, with an opening for the camera.

Bob
by MicroBob
Sun Oct 13, 2019 10:30 pm
Forum: Illumination Techniques
Topic: Diverse views of Pleurosigma on a strew slide
Replies: 8
Views: 318

Re: Diverse views of Pleurosigma on a strew slide

Do you positively identify vibration-noise in the images ? No, not at all, but this would be difficult to identify at this performance level. You would just get varying or sub optimal results without knowing it. So it makes sense to be careful about vibration as far as possible. I think a wobbly de...
by MicroBob
Sun Oct 13, 2019 7:21 pm
Forum: Illumination Techniques
Topic: Incident fluorescence: Ficus leaf tip cross section
Replies: 20
Views: 688

Re: Incident fluorescence: Ficus leaf tip cross section

Hi together, I forgot to report one thing: I usually just pop a Nikon 1j5 with eyepice adapter into a bino port and shoot my pictures. Usually this works well for my not too perfectionist approach. With my improvised fluorescence setup I ran into faily long exposure times like 2 seconds. I saw a str...