MicrobeHunter.com Microscopy Forum

Hi Doron,
while both my 16:1 Neofluar and 40:1 achromat phase objectives are delaminated this one is fine!

Bob

Hi together,
I just bought a nice Neofluar 40 Ph2 phase contrast objective and gave it a try with some plancton mix from my plancton aquaria.
Here two ciliates that could have been captured better with a 100:1 objective but I was too lazy and the dwindling oil resources and all...

Bob

This is a Zeiss Jena Planapochromat 25.... C where "C" means it is intended to be used with a correction eyepiece. This is a very late (and rare) 160mm objective, it has to date from shortly before the appearance of the CF 250 infinity objective series. Zeiss Jena and Zeiss West were close in the ch...

Hi Chris, nice restoration of an interesting microscope! Good that you got rid of the nicotin beige components too. This is a fairly rare microscope with probably few sold units outside of GB and even fewer surviving stands. The objectives turn up frequently on ebay UK so it won't be a problem to ad...

Fantastic microscope! You must have assembled a heap of knowledge and experience befor you were able to identify the fitting parts for this combination, amazing.
And a very nice crystal image too!

Bob

Micro photography is an additional field of learning and work. I would suggest that you read up on the options of choosing and adapting cameras before you spend money. For high quality photography it is very important to have a virbation free shutter release from live view, EFSC, electronic first sh...

Hi together,
here a couple more images from out former member Walter.
Scanned from 35mm slides.

Bob

Hi Don,
can you show more about your sectioning, perhaps a photo of you microtome and specimen?
Your photos look good but I'm not quite sure what I see.

Bob

Hi Mitch, for fluorescence one likes to have bright objectives (comparatively high n.a.) and little autofluorescence. Zeiss recommended their (only relatively plan) Neofluars or simpla Achromats. A plan achromatic or apochromatic objective with it's many lenses and cement layers might not be the bes...

I like the magazine a lot and can't second Malpis criticism. Of cause the approach is a single directional one like in a paper magazine. But this was very much how the internet was when the internt became available for the public around 1995 with lots of websites and little interaction. I was in tou...

Hi Don,
that picture looks quite good.
You posted it in somebody else thread on a different topic and it might be overseen there. It would be better if you opened a new thread for this topic.
I would like to post some questions on this as it is an interesting and unusual topic.

Bob

Hi Pete, this looks very much like a pen used to draw technical drawings that were to be reproduced by a classic optical method. This drawing ink was applied by a pen consisting of vertical tube, a wire with a weight to allow to free the nozzle by shaking, and in the back a labyrith seal that allowe...

Hi, we have moved our microscopy group activities over to an internal forum and do video conferences over a Zoom business account. For my home office work since monday I use microsoft teams which allows video conferences, chat and file sharing too. Generally these systems are in highest demand now s...

Hi Phil, so far Daruosh only has a basic abbe condenser that he could equip with a dark field stop. I would guess that he would have to stop the objective down to n.a. 0,6 or 0,7 before he would get a nice dark field image. It also is a question how the deficiencies of the simple condenser will infl...

To work with your existing condenser and a dark field stop the aperture of the objective will have to reduced quite far, loosing lots of resolution. I would suggest the first get the dark field setup for the lower magnifications to work and continue with the 100:1 objective when you have located a r...

Tube 170mm obective projects at 150mm (160-10) eyepiece older Leitz like 10x and no field number, pick up point 18mm down the tube 170-18=152 Difference 2mm, not dramatic. The biggest difference will be seen in very low power objectives where a bit more refocussing than usual may be needed. So mixin...

I only use microscopes with 160mm tube length of differnt brands. The 160mm Leitz objectives are used with 160m Leitz eyepiexes (like 10x18 designation) or 170mm Leitz eyepieces with an 8mm spacer. Look here: https://www.mikroskopie-forum.de/index.php?topic=11818.msg87737#msg87737 This applies for a...

I actually have a pair of similar Leitz objectives, one marked for 160mm, one for 170mm - no difference. The 170mm objectives were used with eyepieces with a pick up point 18mm down the tube, the 160mm with eyepieces with a pick up point 10mm down the tube. This allows to calculate which objective/t...

In a way a good financial situation can be substituted with good trading knowledge or good technical skills. So there are more ways to the same result. I usually buy stuff cheap from sellers who don't know where the top side of the item is. This often generates some technical repair work, which is f...

He made a point of encouraging the user to keep the unused eye open and to teach the brain to block out what it was imaging. Hi Phil, this works of cause, but the imaged student looks from 30cm or so away! @Bram: Of cause notmeant seriously! It is worthwhile to know which is the leading eye for the...

Hi Pete,
nice ad!
It clearly shows why they got on so well with monocular microscopes in these days. Contrary to us today they keep the head further away and squintingly look though one eyepiece with both eyes. So much practical knowledge is lost, it's a pity!

Bob

As far as I know Leitz was much more willing to develop and build special components than Zeiss. So assembling an Ortholux or Orthoplan collection is quite a task! :shock: I have never seen this attatchmend before. Dorons idea of tempering for 37°C, perhaps in combination with an objective that is p...

Hi, I like the images very well! What is the stain you used on the pine stem and what part of the plant was this specifically? On my monitor the pine stem image appears a bit dark, but this my be my setup. If you would like to improve the fruit fly image you could clean up the background a bit, but ...

10W are 10W - it doesn't make much difference for the microscope istself. But the LED won't last long without a good and well connected heat sink. For my 18W HPH 50 LED epi-illuminator I use a heat sink with slowly turning fan as it runs on full power most of the time. The heat sink should not excee...

I have to find a way to permanently fix the heat sink in place. Hi Daroush, I use M3 or even better M2,5 screws for this with a plastic washer to avoid short ciruits. Owning treading tools in these sizes is quite useful for a microscopist. A good non-professional quality will do for aluminium and m...

This has much in common with an areometer, used to measure the density of car cooling fluid.

Typical single use blades have an included angle between micro bevels of 35°. So the blade has to be inclined to minimum 17,5° plus 1°-5° relief angle.

micro wrote: ↑

Sun Mar 22, 2020 5:55 am

MicroBob wrote: ↑

Sun Mar 22, 2020 5:54 am

Do you have to refocus when you change from eyepiece to camera or are botz parfocal?

I have to refocus

This means that you are not using the objective as intended. This can introduce image degradation. Perhaps you can arrange the camera in a way that makes refocussing unnecessary?

Do you have to refocus when you change from eyepiece to camera or are botz parfocal?

While our topic was "fibres" we had a pre topic on corona virus and hygienic and disinfectant recipes. This was from 10 days ago when it still seemed very likely to have a personal meeting! In the Micscape magazine Mol Smith wrote about disinfectant recipes and suggested to add a teaspoon full of th...