Search found 3154 matches

by MicroBob
Tue Oct 24, 2023 6:52 pm
Forum: Microscopes and optics
Topic: 6 cent coverslips vs 24 cent coverslips
Replies: 3
Views: 1221

Re: 6 cent coverslips vs 24 cent coverslips

0,06$ means a pack of 100 is already at 6$. This is a not cheap for basic coverslips. Maybe you can find a different supplier with better prices and selection. Zeiss offers square cover slips of tight tolerance with a thickness of 0,17 +/- 0,005mm. As mentioned above these will only improve image qu...
by MicroBob
Sat Oct 21, 2023 6:28 pm
Forum: Specimens, samples and slides
Topic: Finally - an OK X-section
Replies: 1
Views: 1650

Re: Finally - an OK X-section

Hi Tony, here you can find a document on paraffin embedding and sectioning worms (in German): http://www.mikrohamburg.de/Tips/Paraffinschnitte_03.pdf For paraffin infiltration of plant material I use round about this method with isopropanol as intermedium and like the results: http://www.klaus-henke...
by MicroBob
Fri Oct 06, 2023 3:45 pm
Forum: Specimens, samples and slides
Topic: Water Sample Disposal
Replies: 6
Views: 3268

Re: Water Sample Disposal

I dispose samples from freshwater ponds simly in one of my pond aquariums. I habe posted here before how I set them up.
by MicroBob
Fri Oct 06, 2023 8:54 am
Forum: Microscopes and optics
Topic: On holiday at at St.Lawrence River, microscope info requested
Replies: 14
Views: 2553

Re: On holiday at at St.Lawrence River, microscope info requested

I wrote a bit about the separation of Zeiss after WW2: https://www.microbehunter.com/microscopy-forum/viewtopic.php?p=110505#p110505 In this situation it must have been quite dramatic to realize that they had developed the W model and it wouldn't fit the market. Maybe they were able to get a much be...
by MicroBob
Thu Oct 05, 2023 5:25 pm
Forum: Microscopes and optics
Topic: On holiday at at St.Lawrence River, microscope info requested
Replies: 14
Views: 2553

Re: On holiday at at St.Lawrence River, microscope info requested

Actually the KF and the GFL are not as similar as their look would suggest. On the KF coarse and fine focus are connected and move the stage, a quit emodern solution. On the GFL they are independent and the coarse focus moves the arm of the microscope, a more traditional setup. Both are very beautif...
by MicroBob
Sat Jul 29, 2023 6:33 pm
Forum: For forum members who want to buy and sell equipment
Topic: A Zeiss Universal III to sell [sold!]
Replies: 7
Views: 1931

Re: A Zeiss Universal III to sell

The Phomi and Universal are a bit unwieldy but actually they take up just one microscope desk place. Only when one tries to lift them and put them elsewhere the wheight and bulk really shows. They don't go for much money and are comfortable and quick to use and top quality. And advanced options are ...
by MicroBob
Sat Mar 11, 2023 6:42 am
Forum: Camera systems and imaging
Topic: A potential Game-Changer
Replies: 25
Views: 6802

Re: A potential Game-Changer

apochronaut wrote:
Thu Mar 09, 2023 3:07 pm
I bought a dedicated microscope camera with s 5 mp sensor and global shutter about a year ago and it is definitely of value when capturing quick moving action on video.
Can you post a link to this camera, Phil? 5 mp sounds a lot less restricted than the 1,6 mp of the raspi camera.

Bob
by MicroBob
Sat Feb 18, 2023 8:53 pm
Forum: Microscopy accessories
Topic: Where to buy circular slide cover?
Replies: 16
Views: 4937

Re: Where to buy circular slide cover?

Here is a recent collection of microscopy material sources, mostly in Germany, it is no problem to get round cover slips: http://www.mikrohamburg.de/Linklisten/Linklisten%202021.pdf With round chinese cover slips I have made the experience that their thickness varied a lot, up to 0,25mm, and they we...
by MicroBob
Fri Feb 10, 2023 11:18 am
Forum: Specimens, samples and slides
Topic: Dammar / Damar resin as slide mountant - how to prepare it
Replies: 7
Views: 3886

Re: Dammar / Damar resin as slide mountant - how to prepare it

The recipe I posted contains the least possible amount of solvent. It is on the border of being too stiff at room temperature. The advantage is that not much shrinkage occurs.
by MicroBob
Wed Feb 08, 2023 8:58 pm
Forum: Specimens, samples and slides
Topic: I hate bubbles.
Replies: 15
Views: 5209

Re: I hate bubbles.

The amount of trouble with bubbles depends on what you do with the mountant, like shaking, carrying it. And it depends on the mountant itself, some hame much more problems with bubbles than others. For plant stem sections Euparal works fine and has little problems with bubbles. What do you do with y...
by MicroBob
Wed Feb 08, 2023 8:41 pm
Forum: Microscopes and optics
Topic: Improving a toy microscope..add a condenser?
Replies: 30
Views: 53797

Re: Improving a toy microscope..add a condenser?

What you want is a lens that offers a light cone with a wide included angle, a lens of e.g. 20-35mm and quite fat in the middle with a short focal lenght.
by MicroBob
Tue Feb 07, 2023 7:20 am
Forum: Microscopes and optics
Topic: Improving a toy microscope..add a condenser?
Replies: 30
Views: 53797

Re: Improving a toy microscope..add a condenser?

Here was a topic on portable microscopes: https://www.microbehunter.com/microscopy-forum/viewtopic.php?f=18&t=7632&hilit=field+microscope+meopta It is not difficult to find one for 200x combined magnification but becomes increasingly dificult to find something light an daffordable with higher magnif...
by MicroBob
Sun Feb 05, 2023 8:04 am
Forum: Microscopes and optics
Topic: Improving a toy microscope..add a condenser?
Replies: 30
Views: 53797

Re: Improving a toy microscope..add a condenser?

I once had a microscope that has cost 40€ new with quite acceptable objectives 4:1, 10:1 and 40:1. It was mainly let down by the very low numeric aperture of the illumination. I installed a small camera objective below the stage, oriented so the front lens faces the light source. This improved image...
by MicroBob
Sat Feb 04, 2023 9:33 pm
Forum: Microscopes and optics
Topic: EM1 portable microscopes
Replies: 13
Views: 4762

Re: EM1 portable microscopes

The EM-1 is advertised over a Kickstarter crowd funding scheme - not very convincing when it is already developed and in production. At least one of the application photos was reused from the Newton homepage with just a little editing of the photo. I always found the Newton nm-1 interesting but it's...
by MicroBob
Sun Jan 29, 2023 2:58 pm
Forum: Beginner's corner
Topic: Help with decision on Leitz Orthoplan
Replies: 51
Views: 9564

Re: Help with decision on Leitz Orthoplan

The main problem with transmitted light fluorescence, which made it outdated long ago, is that it is extremely wasteful of illumination. Fluorescence requires both intense illumination for excitation AND efficient removal of that exciting light such that the fluorescence sensor (eye, camera, whatev...
by MicroBob
Sat Jan 28, 2023 8:27 pm
Forum: Beginner's corner
Topic: Help with decision on Leitz Orthoplan
Replies: 51
Views: 9564

Re: Help with decision on Leitz Orthoplan

As long as you use 170mm eyepieces on your 170mm microscope, both 160mm and 170mm objectives will work ok, as the location of the image only differs by 2mm, as seen here: https://www.microbehunter.com/microscopy-forum/viewtopic.php?p=77060#p77060 I really like the 170mm Pl Fl objectives I've tried,...
by MicroBob
Sat Jan 28, 2023 7:58 am
Forum: Beginner's corner
Topic: Help with decision on Leitz Orthoplan
Replies: 51
Views: 9564

Re: Help with decision on Leitz Orthoplan

Congratulations, you've got a very nice microscope there! The Orthoplan arrived on the market in the 1960s and this was still Leitz 170mm era. They were changed to 160mm later. So when looking for parts it will be important to know which components are compatible. I posted a link to the german forum...
by MicroBob
Sat Dec 31, 2022 2:25 pm
Forum: Microscopes and optics
Topic: Zeiss 4X objective
Replies: 20
Views: 3506

Re: Zeiss 4X objective

Hi Keith, a good and very short objective that fits well to your Zeiss West Standard would be the Leitz EF 4. It is shorter than the Zeiss Plan 2,5. You might already be aware of this but there were two Zeiss companies between 1945 and 1990: Zeiss Jena and Zeiss West (Oberkochen and Göttingen). Ther...
by MicroBob
Sun Dec 25, 2022 7:11 pm
Forum: Specimens, samples and slides
Topic: Dammar / Damar resin as slide mountant - how to prepare it
Replies: 7
Views: 3886

Re: Dammar / Damar resin as slide mountant - how to prepare it

It would be interesting to hear when you try the recipe or adjust the procedure to the equipment you have available. It would also be interesting to hear how well you can get dammar and xylene in your country. One remaining information: Dammar has green fluorescence when excited with blue, like prob...
by MicroBob
Sun Dec 25, 2022 6:59 pm
Forum: Specimens, samples and slides
Topic: Dammar / Damar resin as slide mountant - how to prepare it
Replies: 7
Views: 3886

Re: Dammar / Damar resin as slide mountant - how to prepare it

Since Dammar doesn't dissolve well in alcohols I don't think it is likely that it will fully tolerate alcohol when dissolved in turpentine. In my eyes Dammar is similar in use to canadabalsam, intermedium xylene, not tolerant of water. Compared with canadabalsam I would say it is a bit less variable...
by MicroBob
Sun Dec 25, 2022 4:52 pm
Forum: Specimens, samples and slides
Topic: Dammar / Damar resin as slide mountant - how to prepare it
Replies: 7
Views: 3886

Dammar / Damar resin as slide mountant - how to prepare it

Hi together, quite often the topic arises of which mountants to use and where to get them. Often stuff like nail polish (where does this idea come from? :roll: ) is tried, discovered as not very usable and abandoned. In some countries a variety of proven mountants is available but this is by far not...
by MicroBob
Sun Dec 25, 2022 4:24 pm
Forum: Resources (online, books etc.)
Topic: English pages on www.mikrohamburg.de - lots of micro methods
Replies: 1
Views: 1967

English pages on www.mikrohamburg.de - lots of micro methods

Hi together,
here a link to a lot of descriptions of micro methods on our english pages on www.mikrohamburg.de:
http://www.mikrohamburg.de/HomeTips_E.html

Bob
by MicroBob
Wed Dec 21, 2022 9:13 pm
Forum: Microscopy accessories
Topic: A pocket camera adaptor
Replies: 2
Views: 1587

Re: A pocket camera adaptor

In my experience the lenses of these cameras show defects when used on the microscope that doesn't show in normal photography. This limits the acheivable image quality. This effect comes from the way the plastic lenses are made and from dirt inside the objectives. The problem mostly shows in stronge...
by MicroBob
Wed Dec 21, 2022 11:39 am
Forum: Microscopy accessories
Topic: A home made field microtome
Replies: 1
Views: 1431

Re: A home made field microtome

Thank you for this description, it is a very nice microtome design. What I like best is the combination of carrot-punch and microtome.
If you find need for stiffer blades the Olfa LBB blades are much thicker and equaly sharp.
by MicroBob
Wed Nov 30, 2022 9:46 pm
Forum: Beginner's corner
Topic: Carl Zeiss Standard 14 advice
Replies: 37
Views: 13243

Re: Carl Zeiss Standard 14 advice

Congratulations! The delamination might be outside the field of view or not be well visible anyway. The fine focus gears of this series is much more robust than usual so there is no immediate risk of wrecking it when it moves a little stiff. Condenser rack-is there a nut with two holes? Stage should...
by MicroBob
Wed Nov 30, 2022 3:08 pm
Forum: Beginner's corner
Topic: Carl Zeiss Standard 14 advice
Replies: 37
Views: 13243

Re: Carl Zeiss Standard 14 advice

To compensate for the yellowish light you would need a blue filter.
These original transformers are durable but quite impactical in use. There are ready made LED conversions and it is not that difficult to build one yourself.
by MicroBob
Tue Nov 29, 2022 3:17 am
Forum: Beginner's corner
Topic: Carl Zeiss Standard 14 advice
Replies: 37
Views: 13243

Re: Carl Zeiss Standard 14 advice

I would suggest a lab power supply with adjustable voltage AND current. It can power the bulb as well an an LED in case you do this upgrade later. I'm not sure as I've never used it one, how do you limit the max voltage or current? It seems quite unhandy if one needs to look at the screen while tur...
by MicroBob
Mon Nov 28, 2022 1:17 pm
Forum: Beginner's corner
Topic: Carl Zeiss Standard 14 advice
Replies: 37
Views: 13243

Re: Carl Zeiss Standard 14 advice

I would suggest a lab power supply with adjustable voltage AND current. It can power the bulb as well an an LED in case you do this upgrade later.
by MicroBob
Sat Nov 26, 2022 9:22 am
Forum: Beginner's corner
Topic: Carl Zeiss Standard 14 advice
Replies: 37
Views: 13243

Re: Carl Zeiss Standard 14 advice

As far as I know the delamination tendency was caused by the way the uv-glue was stored and handled, so there would be reason for different levels of delaminating tendency in different products and from different production times, maybe even markets. I myself have found im the stuff I got my hands o...
by MicroBob
Sat Nov 26, 2022 7:05 am
Forum: Beginner's corner
Topic: Carl Zeiss Standard 14 advice
Replies: 37
Views: 13243

Re: Carl Zeiss Standard 14 advice

Basic Zeiss West optics don't tend to delaminate much, it is more the better corrected objectives that have this problem. On the other hand side for most other systems there never was a Planapo 63 /1,4.. Leitz earlier used 37mm objectives, then 45mm objectives. The 45mm objectives 170 and 160mm can ...