Search found 92 matches

by tgss
Thu Dec 12, 2019 12:17 pm
Forum: Pictures and Videos
Topic: Navicula lyra
Replies: 15
Views: 445

Re: Navicula lyra

Caught up with this one rather late. Amazing detail and contrast and beautifully sharp. Very well done - again.
Tom W.
by tgss
Thu Dec 05, 2019 12:23 pm
Forum: Pictures and Videos
Topic: Stauroneis phoenicenteron (COL-DIC)
Replies: 4
Views: 194

Re: Stauroneis phoenicenteron (COL-DIC)

Not a diatomist by any means, but based on many other images I've seen I'd say the COL is definitely an improvement.
Tom W.
by tgss
Wed Dec 04, 2019 3:09 pm
Forum: Pictures and Videos
Topic: Stauroneis phoenicenteron (COL-DIC)
Replies: 4
Views: 194

Re: Stauroneis phoenicenteron (COL-DIC)

Very nice 75RR. I note that your 63x doesn't seem to be wearing out with use - still producing just as good images as ever! The lower image in particular is very good.
Tom W.
by tgss
Fri Nov 29, 2019 12:48 pm
Forum: My microscope
Topic: When You're Hot, You're Hot. Looky Here What I Got!
Replies: 7
Views: 327

Re: When You're Hot, You're Hot. Looky Here What I Got!

+1 on the Ortho-Illuminators - very, very sexy!
Tom W
by tgss
Thu Nov 28, 2019 1:17 pm
Forum: Resources (online, books etc.)
Topic: The Freshwater Microscopist by Tony Pattinson
Replies: 7
Views: 2470

Re: The Freshwater Microscopist by Tony Pattinson

Thanks for the update on Part 4 of the series Kurt. I have the other three, but wasn't aware that Tony had released a fourth. As you stated, the books are a terrific source of thoroughly practical ideas and procedures, with attractive and informative illustrations. Tony is a bit of a Vickers M41 fan...
by tgss
Sun Nov 17, 2019 2:10 am
Forum: Miscellaneous
Topic: Unlocking Nature's Secrets: The Serengeti Rules:
Replies: 5
Views: 327

Re: Unlocking Nature's Secrets: The Serengeti Rules:

It's apparently available on Amazon Prime video, to rent - $4.99, or buy - $5.99, if that helps anyone.

Tom W.
by tgss
Tue Nov 12, 2019 7:20 pm
Forum: Camera systems and imaging
Topic: HA2307 HDMI industrial camera
Replies: 39
Views: 5320

Re: HA2307 HDMI industrial camera

Hi 75RR I'm beginning to feel guilty about hijacking Radazz's post with this but... I agree that you need to know what the pixel size really is in order to compare it with what is needed. I guess, for me at least, the discussion boils down to the recognition that, in order to figure out what pixel s...
by tgss
Tue Nov 12, 2019 6:20 pm
Forum: Camera systems and imaging
Topic: HA2307 HDMI industrial camera
Replies: 39
Views: 5320

Re: HA2307 HDMI industrial camera

Hi 75RR I think the misunderstanding is because I used an unfortunate term, pixel density, since it implies pixels/mm^2, or some such, whereas I was using it the sense of "number of pixels/sensor". That's because I'm an idiot. :oops: The intent was to get around the issue of different sized sensors ...
by tgss
Tue Nov 12, 2019 4:34 pm
Forum: Camera systems and imaging
Topic: HA2307 HDMI industrial camera
Replies: 39
Views: 5320

Re: HA2307 HDMI industrial camera

The pixel size should be 3 or 4 times smaller that the projected size of the object you are trying to capture. See link and the extract below: https://www.lumenera.com/blog/understanding-resolution-in-digital-microscopy please note that when they say: " largest possible pixel size required to fully...
by tgss
Tue Nov 12, 2019 3:12 pm
Forum: Camera systems and imaging
Topic: HA2307 HDMI industrial camera
Replies: 39
Views: 5320

Re: HA2307 HDMI industrial camera

I've been a little perplexed while following this thread. The idea that resolution depends primarily on pixel size is the idea that's causing me problems. I admit that "primarily" has not been explicitly stated, but seems to be implicit in the arguments. If carried to extremes this would imply that ...
by tgss
Wed Nov 06, 2019 12:58 pm
Forum: About the website, the magazine and this forum
Topic: Vetting newcomers
Replies: 15
Views: 1383

Re: Vetting newcomers

Well... I'm gobsmacked! I couldn't understand what was going on when I read billbilt's posts - thought there must have been some kind of malfunction on the forum, until I read the following posts and realised it was for real. Very, very disappointed - really undermines the forum and leaves a bad tas...
by tgss
Thu Oct 24, 2019 7:18 pm
Forum: Microscopes and optics
Topic: Taking apart Jena Docuval
Replies: 2
Views: 500

Re: Taking apart Jena Docuval

Very unfortunate that this instrument was trashed Piter_. I wish you every success in restoring it to fully working condition.
Tom W.
by tgss
Sun Oct 20, 2019 10:31 pm
Forum: Pictures and Videos
Topic: Marine Alga
Replies: 7
Views: 751

Re: Marine Alga

What mrsonchus said :!: .

Tom W.
by tgss
Sat Oct 19, 2019 10:42 pm
Forum: Microscopes and optics
Topic: Determining the molecular organisation of biological materials using polarized light microscopy
Replies: 6
Views: 763

Re: Determining the molecular organisation of biological materials using polarized light microscopy

I used a 10x objective, 10x eyepiece and 1.6x optovar magnification, both images were cropped from stacked photos. Later I can take some images of a stage micrometer and calculate the number of pixels per graduation to generate a scale bar. Thank you, Wes, for the clarification. No need to generate...
by tgss
Fri Oct 18, 2019 11:31 pm
Forum: Microscopes and optics
Topic: Determining the molecular organisation of biological materials using polarized light microscopy
Replies: 6
Views: 763

Re: Determining the molecular organisation of biological materials using polarized light microscopy

Fascinating stuff Wes, excellent images and a very clear exposition of the physics of the process. Can you give an idea of the scale of the images, or at least the objective powers used?
Thanks for sharing.
Tom W.
by tgss
Wed Oct 02, 2019 8:31 pm
Forum: Beginner's corner
Topic: Mounting media
Replies: 5
Views: 673

Re: Mounting media

Hi Aslan You might take a look Electron Microscopy Sciences. In particular https://www.emsdiasum.com/microscopy/products/histology/mounting_media.aspx#17997 . They are based in the states, but I have ordered from them in the past and received very good service. Many of their products, though apparen...
by tgss
Wed Oct 02, 2019 8:13 pm
Forum: Off Topic
Topic: The Explorer's Guide to Biology
Replies: 2
Views: 1669

Re: The Explorer's Guide to Biology

Looks like this might be very interesting. Thank you for the link.
Tom W.
by tgss
Sun Sep 29, 2019 2:51 pm
Forum: Pictures and Videos
Topic: Colurella sp
Replies: 14
Views: 960

Re: Colurella sp

I think there's a lesson here for all of us 75. It seems that with only one microscope, and only one objective, and one or two sampling locations, it's possible to keep turning out remarkable images! I appreciate it's not quite as simple as that of course, but it does cause one to pause and think a ...
by tgss
Sun Sep 08, 2019 3:13 pm
Forum: Home-made microscope adaptations
Topic: EOS 4000D modification
Replies: 5
Views: 799

Re: EOS 4000D modification

Thanks for that Dave. I was about to make a dummy battery and install it using a similar slot for the cable, something I've previously done on a camcorder for my son. I didn't realize that dummy batteries were commercially available but will certainly make use of one now. Tom W. This is the one I b...
by tgss
Sun Sep 08, 2019 11:54 am
Forum: Home-made microscope adaptations
Topic: EOS 4000D modification
Replies: 5
Views: 799

Re: EOS 4000D modification

Thanks for that Dave. I was about to make a dummy battery and install it using a similar slot for the cable, something I've previously done on a camcorder for my son. I didn't realize that dummy batteries were commercially available but will certainly make use of one now.
Tom W.
by tgss
Fri Sep 06, 2019 10:19 pm
Forum: Pictures and Videos
Topic: Jellyfish gravity sensing organs
Replies: 7
Views: 844

Re: Jellyfish gravity sensing organs

Very informative Wes, and excellent images. Thank you.
Tom W.
by tgss
Thu Sep 05, 2019 11:23 am
Forum: Pictures and Videos
Topic: Rotifer and some algae
Replies: 12
Views: 1218

Re: Rotifer and some algae

Thank you! Well, the body was still, but the head moved. Therefore the head is barely visible except for the eye and the jaws. These I managed to get sharp in a couple of frames: The rest of the head not. If I plan for shooting rotifers, I use glutaraldehyde before I put on the cover glass. With ri...
by tgss
Thu Sep 05, 2019 1:08 am
Forum: Pictures and Videos
Topic: Marine worm (possibly Platynereis sp)
Replies: 5
Views: 566

Re: Marine worm (possibly Platynereis sp)

I agree with mintakax, interesting looking worm. So many interesting creatures in the sea. I grew up by the sea, but now live by the great lakes. They too are impressive, but I still miss the sea.
Tom W.
by tgss
Thu Sep 05, 2019 1:03 am
Forum: Pictures and Videos
Topic: Rotifer and some algae
Replies: 12
Views: 1218

Re: Rotifer and some algae

Wonderful images hkv. Thanks for sharing.
I'm intrigued by the rotifer image in that, despite stacking and stitching, there is very little evidence of movement. Is there a secret you're willing to share??
Tom W.
by tgss
Wed Aug 28, 2019 11:29 am
Forum: Resources (online, books etc.)
Topic: Protocols in Protozoology
Replies: 3
Views: 1062

Re: Protocols in Protozoology

Very interesting link Wes. Bedtime reading for months! Thank you.
Tom W.
by tgss
Mon Aug 26, 2019 10:53 pm
Forum: Pictures and Videos
Topic: Short Hand-Sectioning Demo with video
Replies: 2
Views: 885

Re: Short Hand-Sectioning Demo with video

Excellent John. Thank you.
Tom W.
by tgss
Mon Aug 26, 2019 1:35 am
Forum: Pictures and Videos
Topic: Pollen. There's lots of it about.
Replies: 99
Views: 30558

Re: Pollen. There's lots of it about.

I heartily agree with Bob. The collage is very attractive and informative. In fact if all three images in the collage above were of the same plant it would be perfect, and could even be extended, within limits, to include other details. As for the unknown, I've no idea, except that it definitely loo...
by tgss
Sun Aug 25, 2019 5:56 pm
Forum: Pictures and Videos
Topic: Pollen. There's lots of it about.
Replies: 99
Views: 30558

Re: Pollen. There's lots of it about.

Sorry Alan. Pictures failed to show up again (for me at least).
Tom W.
by tgss
Sat Aug 24, 2019 6:32 pm
Forum: Home-made microscope adaptations
Topic: Home made (Very Basic) Microtome
Replies: 42
Views: 4589

Re: Home made (Very Basic) Microtome

Good job all round Alan! Pretty soon John B is going to have to look to his laurels ;)
Tom W.
by tgss
Tue Aug 20, 2019 2:16 pm
Forum: Home-made microscope adaptations
Topic: Home made (Very Basic) Microtome
Replies: 42
Views: 4589

Re: Home made (Very Basic) Microtome

Back when I was building and flying F1D Indoor Free Flight, I learned to dissect Gillette Mach 3 razor heads for the individual blades, which I super-glued to stick handles, to cut microfilm for wings and tail surfaces. Tiny little things are a bit of trick to handle, but even sharper yet. Nice to ...