MicrobeHunter.com Microscopy Forum

What mrsonchus said .

Tom W.

I used a 10x objective, 10x eyepiece and 1.6x optovar magnification, both images were cropped from stacked photos. Later I can take some images of a stage micrometer and calculate the number of pixels per graduation to generate a scale bar. Thank you, Wes, for the clarification. No need to generate...

Fascinating stuff Wes, excellent images and a very clear exposition of the physics of the process. Can you give an idea of the scale of the images, or at least the objective powers used?
Thanks for sharing.
Tom W.

Hi Aslan You might take a look Electron Microscopy Sciences. In particular https://www.emsdiasum.com/microscopy/products/histology/mounting_media.aspx#17997 . They are based in the states, but I have ordered from them in the past and received very good service. Many of their products, though apparen...

Looks like this might be very interesting. Thank you for the link.
Tom W.

I think there's a lesson here for all of us 75. It seems that with only one microscope, and only one objective, and one or two sampling locations, it's possible to keep turning out remarkable images! I appreciate it's not quite as simple as that of course, but it does cause one to pause and think a ...

Thanks for that Dave. I was about to make a dummy battery and install it using a similar slot for the cable, something I've previously done on a camcorder for my son. I didn't realize that dummy batteries were commercially available but will certainly make use of one now. Tom W. This is the one I b...

Thanks for that Dave. I was about to make a dummy battery and install it using a similar slot for the cable, something I've previously done on a camcorder for my son. I didn't realize that dummy batteries were commercially available but will certainly make use of one now.
Tom W.

Very informative Wes, and excellent images. Thank you.
Tom W.

Thank you! Well, the body was still, but the head moved. Therefore the head is barely visible except for the eye and the jaws. These I managed to get sharp in a couple of frames: The rest of the head not. If I plan for shooting rotifers, I use glutaraldehyde before I put on the cover glass. With ri...

I agree with mintakax, interesting looking worm. So many interesting creatures in the sea. I grew up by the sea, but now live by the great lakes. They too are impressive, but I still miss the sea.
Tom W.

Wonderful images hkv. Thanks for sharing.
I'm intrigued by the rotifer image in that, despite stacking and stitching, there is very little evidence of movement. Is there a secret you're willing to share??
Tom W.

Very interesting link Wes. Bedtime reading for months! Thank you.
Tom W.

Excellent John. Thank you.
Tom W.

I heartily agree with Bob. The collage is very attractive and informative. In fact if all three images in the collage above were of the same plant it would be perfect, and could even be extended, within limits, to include other details. As for the unknown, I've no idea, except that it definitely loo...

Sorry Alan. Pictures failed to show up again (for me at least).
Tom W.

Good job all round Alan! Pretty soon John B is going to have to look to his laurels
Tom W.

Back when I was building and flying F1D Indoor Free Flight, I learned to dissect Gillette Mach 3 razor heads for the individual blades, which I super-glued to stick handles, to cut microfilm for wings and tail surfaces. Tiny little things are a bit of trick to handle, but even sharper yet. Nice to ...

Good job Alan... actually very good job considering the vast array of tooling you had at your disposal And as you have shown, you never know what little bonus (oblique) fate will bring your way if you only try!
Tom W.

I did wonder about that myself Bill. We are assured that they cannot reproduce on the moon, which seems reasonable, but we also know they can survive (remain viable) in the vacuum of space for a very long time. However I suspect even tardigrades have a best before date which we can only hope will be...

Thank you John B. First class images.
Tom W.

Another beautiful image - thanks again for posting!
Tom

Welcome Chris - the more the merrier! Can you tell us something about your microscopes perhaps in the "My Microscopes" forum?
Tom

Hi Bob I sympathise with your concerns regarding external image hosting sites and the big problems that can be created when they disappear, permanently or temporararily. Your suggestion that some money be collected to support additional storage space is OK with me. But I don't know how the forum hos...

Glad it worked OK Alan, and the process is simple. Should be a very good option to the other, limited storage, hosting services, and an encouragement to minimize space useage on the forum. Thanks for all the effort you have put into it.
Tom W.

OK. Here is test image



Tom W.

Seems to be working fine Alan. Well done! I tried to carry out a test but have not received a password from postimages - my ISP's mail server is having trouble with passwords right now. Will try again later.
Tom W

Can see three images in your first post, but cannot see image in your third post.
Tom

I am. I'm especially dim-witted! I cleared my browser cache and am no longer special - I see the first two images, but not the third.
Tom

For what it's worth, at this point in time, I can see all three images.
Tom