Search found 121 matches

by tgss
Sat May 30, 2020 11:27 am
Forum: Pictures and Videos
Topic: My First Video - Soap Bubbles!
Replies: 15
Views: 731

Re: My First Video - Soap Bubbles!

Very nice video indeed. Nascent cell membranes??
Tom W.
by tgss
Thu May 14, 2020 1:50 am
Forum: Illumination Techniques
Topic: Still Looking into Hoffman Optics
Replies: 3
Views: 629

Re: Still Looking into Hoffman Optics

Hi ImperatorRex The Mikrokosmos articles appear to be very good, but they are a difficult job for a non-German speaker to translate, even with the help of Google or Microsoft translation services. I have attempts at translating them tucked away somewhere but the translations are not very lucid. But ...
by tgss
Wed May 13, 2020 11:32 am
Forum: Illumination Techniques
Topic: Still Looking into Hoffman Optics
Replies: 3
Views: 629

Re: Still Looking into Hoffman Optics

Thanks for the link microb. Very useful background information.
Tom W.
by tgss
Wed May 13, 2020 11:29 am
Forum: Home-made microscope adaptations
Topic: A fun one for the DIY set: pantographic micromanipulator
Replies: 3
Views: 618

Re: A fun one for the DIY set: pantographic micromanipulator

Excellent job Bram. Really nicely done.
Tom W.
by tgss
Wed Apr 22, 2020 11:59 am
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 4382

Re: Pollen Fine Detail

Excellent, excellent, excellent! Wonderful images. And you are absolutely correct - there's no need to be envious of super resolution SEM images when we can be envious of mrsonchus' LM images :mrgreen: Tom W P.S. The dandelions in my garden have no need to worry... yet, since they haven't yet appear...
by tgss
Sun Apr 19, 2020 11:22 am
Forum: Pictures and Videos
Topic: Stomata in tulip leaf
Replies: 15
Views: 920

Re: Stomata in tulip leaf

Very interesting series of posts. Thanks for starting it off, MicroBob, with your example of a variation on an old method that appears to give excellent results.
Tom W.
by tgss
Thu Apr 09, 2020 11:03 pm
Forum: Microscopy accessories
Topic: The most important microscope accessory
Replies: 11
Views: 1796

Re: The most important microscope accessory

Wow BramHuntingNematodes that is one shiny, shiny tabletop! I like it very much.
Tom W.
by tgss
Tue Apr 07, 2020 7:53 pm
Forum: Pictures and Videos
Topic: Pine needle-fluorescence,
Replies: 11
Views: 564

Re: Pine needle-fluorescence,

Thank you. Great images. In the latter three images, which I assume to be with acridine orange, there appears to be a superb differentiation of tissues, if in fact the various colours actually represent different tissue types. Is that actually the case? Tom W. Yes ,acridine staining make differenti...
by tgss
Tue Apr 07, 2020 11:18 am
Forum: Pictures and Videos
Topic: Pine needle-fluorescence,
Replies: 11
Views: 564

Re: Pine needle-fluorescence,

Great images. In the latter three images, which I assume to be with acridine orange, there appears to be a superb differentiation of tissues, if in fact the various colours actually represent different tissue types. Is that actually the case?
Tom W.
by tgss
Sun Apr 05, 2020 11:10 pm
Forum: Pictures and Videos
Topic: Pine needle-safranin,fast green staining.
Replies: 10
Views: 467

Re: Pine needle-safranin,fast green staining.

Seb28 wrote:
Sun Apr 05, 2020 8:29 pm
Hi Seb
Can you tell us what PEG molecular weight you are using for the hand sectioned samples?
I'm using PEG1500.
Thanks Seb. Sorry for wasting your time! I already edited my post to tell myself it was 1500, as stated in your original post. Duh!!
Tom W.
by tgss
Sun Apr 05, 2020 7:12 pm
Forum: Pictures and Videos
Topic: Pine needle-safranin,fast green staining.
Replies: 10
Views: 467

Re: Pine needle-safranin,fast green staining.

Hi Seb
Can you tell us what PEG molecular weight you are using for the hand sectioned samples?
Regards
Tom W.

Edit: OK - brain fade - I just re-read the post - dilute 1500 is the answer! (Or is it 42?)
by tgss
Sun Apr 05, 2020 11:38 am
Forum: Pictures and Videos
Topic: Pine needle-safranin,fast green staining.
Replies: 10
Views: 467

Re: Pine needle-safranin,fast green staining.

Very nice result indeed Seb. From your description it sounds like you cut the section by hand. If so you did an excellent job.
Tom W.
by tgss
Fri Apr 03, 2020 11:52 am
Forum: Specimens, samples and slides
Topic: Apple Twig Hand Section, Stain & Mount
Replies: 9
Views: 672

Re: Apple Twig Hand Section, Stain & Mount

Excellent, informative, visually compelling, keep 'em coming!
Tom W.
by tgss
Sun Mar 29, 2020 11:39 pm
Forum: Off Topic
Topic: Micscape online Magazine
Replies: 27
Views: 7528

Re: Micscape online Magazine

+1 re: MichaelG's post.

I have had some problems with some of the articles Mr. Smith has published on Micscape in the past. I think the current article is the most problematic to date.
Tom W
by tgss
Sat Mar 28, 2020 7:04 pm
Forum: Pictures and Videos
Topic: Viridiraptor
Replies: 8
Views: 839

Re: Viridiraptor

Beautifully done sinabro. Thanks for sharing.
Tom W.
by tgss
Sat Mar 28, 2020 2:03 pm
Forum: Specimens, samples and slides
Topic: Whole Mount Insect Slide Preparation Part 2
Replies: 7
Views: 1121

Re: Whole Mount Insect Slide Preparation Part 2

Hi Antoni
+1 re: daruosha's post, and don't worry about the delay - we appreciate the time and trouble you have taken to put this together.
Tom W
by tgss
Tue Mar 24, 2020 11:48 am
Forum: Miscellaneous
Topic: Large SEM of Paralia sulcata
Replies: 7
Views: 1009

Re: Large SEM of Paralia sulcata

Think not what you can't see that the SEM can, but what you can see that you couldn't before you had a light microscope.
Tom W.
by tgss
Sun Mar 22, 2020 3:54 pm
Forum: Pictures and Videos
Topic: Whole Mount Insect Slide Preparation
Replies: 5
Views: 1050

Re: Whole Mount Insect Slide Preparation

Thank you AntoniScott. Very interesting video. Really good to see these things done rather than just reading a description which, no matter how detailed, can never capture the nuances. Looking forward to part 2!
Tom W.
by tgss
Fri Mar 20, 2020 11:43 am
Forum: Miscellaneous
Topic: animal-without-mitochondria
Replies: 4
Views: 6318

Re: animal-without-mitochondria

Thanks for the link 75. Very interesting article.
Tom W.
by tgss
Thu Mar 19, 2020 4:29 pm
Forum: Pictures and Videos
Topic: Toxarium undulatum
Replies: 13
Views: 2597

Re: Toxarium undulatum

Amazing! That last image really shows the "undulatum" feature very well.
Tom W.
by tgss
Thu Feb 20, 2020 8:45 pm
Forum: Pictures and Videos
Topic: Trachyneis aspera (Girdle view)
Replies: 8
Views: 1728

Re: Trachyneis aspera (Girdle view)

Ahhhh... thank you 75RR. Of course they are bees, now that I look at them more closely :cry: :oops: They still seem familiar, but I'll just put that down to my continuing decline into senility.
Tom W.
by tgss
Thu Feb 20, 2020 1:40 pm
Forum: Pictures and Videos
Topic: Trachyneis aspera (Girdle view)
Replies: 8
Views: 1728

Re: Trachyneis aspera (Girdle view)

Another beaut 75RR... very nicely done.
Changing the topic slightly, can you tell us about your little fly logo? I have this strong feeling I've seen it before somewhere, possibly in connection with a mathematical problem, but that might just be my geriatric mind wandering!
Tom W.
by tgss
Mon Feb 17, 2020 12:18 pm
Forum: Pictures and Videos
Topic: Pine stem cross section
Replies: 10
Views: 2355

Re: Pine stem cross section

I'm with Wes. Let's hear the details. Very nice result.
Tom W.
by tgss
Fri Feb 14, 2020 12:49 pm
Forum: Pictures and Videos
Topic: Surirella gemma
Replies: 10
Views: 3671

Re: Surirella gemma

Wonderful image. That 63x just never wears out!
Tom W.
by tgss
Sun Feb 09, 2020 12:20 pm
Forum: Introduce yourself
Topic: Hello from Ontario,Canada.
Replies: 6
Views: 3051

Re: Hello from Ontario,Canada.

Welcome scottybee from another Ontarian. I'm sure you'll find this a worthwhile place to hang out.
Tom W.
by tgss
Thu Feb 06, 2020 12:22 pm
Forum: Pictures and Videos
Topic: Micrographs in Black & White
Replies: 22
Views: 4716

Re: Micrographs in Black & White

Excellent!!!!!!!!!!!!!!!!!
Tom W
by tgss
Tue Jan 28, 2020 3:22 pm
Forum: Specimens, samples and slides
Topic: Best adhesive for glass to plastic and glass to glass
Replies: 5
Views: 2018

Re: Best adhesive for glass to plastic and glass to glass

Depending on your intended use, if you decide to try silicone sealant, you should be careful about which type you use. Most sealants intended for household use, e.g. bathtub sealant etc., are compounded with antifungals, which can be harmful to living organisms. There are sealants made without these...
by tgss
Sat Jan 04, 2020 9:01 pm
Forum: Pictures and Videos
Topic: Acridine orange adventures
Replies: 9
Views: 1438

Re: Acridine orange adventures

Awesome shots Wes!
tgss
by tgss
Sun Dec 29, 2019 11:49 pm
Forum: Pictures and Videos
Topic: Daisy flower bud cross section - Bob's first paraffin embedding
Replies: 9
Views: 1178

Re: Daisy flower bud cross section - Bob's first paraffin embedding

Very nicely done Bob. I'll definitely be following you along on this journey.
Tom W.
by tgss
Thu Dec 12, 2019 12:17 pm
Forum: Pictures and Videos
Topic: Navicula lyra
Replies: 15
Views: 2216

Re: Navicula lyra

Caught up with this one rather late. Amazing detail and contrast and beautifully sharp. Very well done - again.
Tom W.