MicrobeHunter.com Microscopy Forum

Very nice video indeed. Nascent cell membranes??
Tom W.

Hi ImperatorRex The Mikrokosmos articles appear to be very good, but they are a difficult job for a non-German speaker to translate, even with the help of Google or Microsoft translation services. I have attempts at translating them tucked away somewhere but the translations are not very lucid. But ...

Thanks for the link microb. Very useful background information.
Tom W.

Excellent job Bram. Really nicely done.
Tom W.

Excellent, excellent, excellent! Wonderful images. And you are absolutely correct - there's no need to be envious of super resolution SEM images when we can be envious of mrsonchus' LM images :mrgreen: Tom W P.S. The dandelions in my garden have no need to worry... yet, since they haven't yet appear...

Very interesting series of posts. Thanks for starting it off, MicroBob, with your example of a variation on an old method that appears to give excellent results.
Tom W.

Wow BramHuntingNematodes that is one shiny, shiny tabletop! I like it very much.
Tom W.

Thank you. Great images. In the latter three images, which I assume to be with acridine orange, there appears to be a superb differentiation of tissues, if in fact the various colours actually represent different tissue types. Is that actually the case? Tom W. Yes ,acridine staining make differenti...

Great images. In the latter three images, which I assume to be with acridine orange, there appears to be a superb differentiation of tissues, if in fact the various colours actually represent different tissue types. Is that actually the case?
Tom W.

Seb28 wrote: ↑

Sun Apr 05, 2020 8:29 pm

Hi Seb
Can you tell us what PEG molecular weight you are using for the hand sectioned samples?

I'm using PEG1500.

Thanks Seb. Sorry for wasting your time! I already edited my post to tell myself it was 1500, as stated in your original post. Duh!!
Tom W.

Hi Seb
Can you tell us what PEG molecular weight you are using for the hand sectioned samples?
Regards
Tom W.

Edit: OK - brain fade - I just re-read the post - dilute 1500 is the answer! (Or is it 42?)

Very nice result indeed Seb. From your description it sounds like you cut the section by hand. If so you did an excellent job.
Tom W.

Excellent, informative, visually compelling, keep 'em coming!
Tom W.

+1 re: MichaelG's post.

I have had some problems with some of the articles Mr. Smith has published on Micscape in the past. I think the current article is the most problematic to date.
Tom W

Beautifully done sinabro. Thanks for sharing.
Tom W.

Hi Antoni
+1 re: daruosha's post, and don't worry about the delay - we appreciate the time and trouble you have taken to put this together.
Tom W

Think not what you can't see that the SEM can, but what you can see that you couldn't before you had a light microscope.
Tom W.

Thank you AntoniScott. Very interesting video. Really good to see these things done rather than just reading a description which, no matter how detailed, can never capture the nuances. Looking forward to part 2!
Tom W.

Thanks for the link 75. Very interesting article.
Tom W.

Amazing! That last image really shows the "undulatum" feature very well.
Tom W.

Ahhhh... thank you 75RR. Of course they are bees, now that I look at them more closely They still seem familiar, but I'll just put that down to my continuing decline into senility.
Tom W.

Another beaut 75RR... very nicely done.
Changing the topic slightly, can you tell us about your little fly logo? I have this strong feeling I've seen it before somewhere, possibly in connection with a mathematical problem, but that might just be my geriatric mind wandering!
Tom W.

I'm with Wes. Let's hear the details. Very nice result.
Tom W.

Wonderful image. That 63x just never wears out!
Tom W.

Welcome scottybee from another Ontarian. I'm sure you'll find this a worthwhile place to hang out.
Tom W.

Excellent!!!!!!!!!!!!!!!!!
Tom W

Depending on your intended use, if you decide to try silicone sealant, you should be careful about which type you use. Most sealants intended for household use, e.g. bathtub sealant etc., are compounded with antifungals, which can be harmful to living organisms. There are sealants made without these...

Awesome shots Wes!
tgss

Very nicely done Bob. I'll definitely be following you along on this journey.
Tom W.

Caught up with this one rather late. Amazing detail and contrast and beautifully sharp. Very well done - again.
Tom W.