Caught up with this one rather late. Amazing detail and contrast and beautifully sharp. Very well done - again.
Tom W.
Search found 92 matches
- Thu Dec 12, 2019 12:17 pm
- Forum: Pictures and Videos
- Topic: Navicula lyra
- Replies: 15
- Views: 445
- Thu Dec 05, 2019 12:23 pm
- Forum: Pictures and Videos
- Topic: Stauroneis phoenicenteron (COL-DIC)
- Replies: 4
- Views: 194
Re: Stauroneis phoenicenteron (COL-DIC)
Not a diatomist by any means, but based on many other images I've seen I'd say the COL is definitely an improvement.
Tom W.
Tom W.
- Wed Dec 04, 2019 3:09 pm
- Forum: Pictures and Videos
- Topic: Stauroneis phoenicenteron (COL-DIC)
- Replies: 4
- Views: 194
Re: Stauroneis phoenicenteron (COL-DIC)
Very nice 75RR. I note that your 63x doesn't seem to be wearing out with use - still producing just as good images as ever! The lower image in particular is very good.
Tom W.
Tom W.
- Fri Nov 29, 2019 12:48 pm
- Forum: My microscope
- Topic: When You're Hot, You're Hot. Looky Here What I Got!
- Replies: 7
- Views: 327
Re: When You're Hot, You're Hot. Looky Here What I Got!
+1 on the Ortho-Illuminators - very, very sexy!
Tom W
Tom W
- Thu Nov 28, 2019 1:17 pm
- Forum: Resources (online, books etc.)
- Topic: The Freshwater Microscopist by Tony Pattinson
- Replies: 7
- Views: 2470
Re: The Freshwater Microscopist by Tony Pattinson
Thanks for the update on Part 4 of the series Kurt. I have the other three, but wasn't aware that Tony had released a fourth. As you stated, the books are a terrific source of thoroughly practical ideas and procedures, with attractive and informative illustrations. Tony is a bit of a Vickers M41 fan...
- Sun Nov 17, 2019 2:10 am
- Forum: Miscellaneous
- Topic: Unlocking Nature's Secrets: The Serengeti Rules:
- Replies: 5
- Views: 327
Re: Unlocking Nature's Secrets: The Serengeti Rules:
It's apparently available on Amazon Prime video, to rent - $4.99, or buy - $5.99, if that helps anyone.
Tom W.
Tom W.
- Tue Nov 12, 2019 7:20 pm
- Forum: Camera systems and imaging
- Topic: HA2307 HDMI industrial camera
- Replies: 39
- Views: 5320
Re: HA2307 HDMI industrial camera
Hi 75RR I'm beginning to feel guilty about hijacking Radazz's post with this but... I agree that you need to know what the pixel size really is in order to compare it with what is needed. I guess, for me at least, the discussion boils down to the recognition that, in order to figure out what pixel s...
- Tue Nov 12, 2019 6:20 pm
- Forum: Camera systems and imaging
- Topic: HA2307 HDMI industrial camera
- Replies: 39
- Views: 5320
Re: HA2307 HDMI industrial camera
Hi 75RR I think the misunderstanding is because I used an unfortunate term, pixel density, since it implies pixels/mm^2, or some such, whereas I was using it the sense of "number of pixels/sensor". That's because I'm an idiot. :oops: The intent was to get around the issue of different sized sensors ...
- Tue Nov 12, 2019 4:34 pm
- Forum: Camera systems and imaging
- Topic: HA2307 HDMI industrial camera
- Replies: 39
- Views: 5320
Re: HA2307 HDMI industrial camera
The pixel size should be 3 or 4 times smaller that the projected size of the object you are trying to capture. See link and the extract below: https://www.lumenera.com/blog/understanding-resolution-in-digital-microscopy please note that when they say: " largest possible pixel size required to fully...
- Tue Nov 12, 2019 3:12 pm
- Forum: Camera systems and imaging
- Topic: HA2307 HDMI industrial camera
- Replies: 39
- Views: 5320
Re: HA2307 HDMI industrial camera
I've been a little perplexed while following this thread. The idea that resolution depends primarily on pixel size is the idea that's causing me problems. I admit that "primarily" has not been explicitly stated, but seems to be implicit in the arguments. If carried to extremes this would imply that ...
- Wed Nov 06, 2019 12:58 pm
- Forum: About the website, the magazine and this forum
- Topic: Vetting newcomers
- Replies: 15
- Views: 1383
Re: Vetting newcomers
Well... I'm gobsmacked! I couldn't understand what was going on when I read billbilt's posts - thought there must have been some kind of malfunction on the forum, until I read the following posts and realised it was for real. Very, very disappointed - really undermines the forum and leaves a bad tas...
- Thu Oct 24, 2019 7:18 pm
- Forum: Microscopes and optics
- Topic: Taking apart Jena Docuval
- Replies: 2
- Views: 500
Re: Taking apart Jena Docuval
Very unfortunate that this instrument was trashed Piter_. I wish you every success in restoring it to fully working condition.
Tom W.
Tom W.
- Sun Oct 20, 2019 10:31 pm
- Forum: Pictures and Videos
- Topic: Marine Alga
- Replies: 7
- Views: 751
Re: Marine Alga
What mrsonchus said
.
Tom W.

Tom W.
- Sat Oct 19, 2019 10:42 pm
- Forum: Microscopes and optics
- Topic: Determining the molecular organisation of biological materials using polarized light microscopy
- Replies: 6
- Views: 763
Re: Determining the molecular organisation of biological materials using polarized light microscopy
I used a 10x objective, 10x eyepiece and 1.6x optovar magnification, both images were cropped from stacked photos. Later I can take some images of a stage micrometer and calculate the number of pixels per graduation to generate a scale bar. Thank you, Wes, for the clarification. No need to generate...
- Fri Oct 18, 2019 11:31 pm
- Forum: Microscopes and optics
- Topic: Determining the molecular organisation of biological materials using polarized light microscopy
- Replies: 6
- Views: 763
Re: Determining the molecular organisation of biological materials using polarized light microscopy
Fascinating stuff Wes, excellent images and a very clear exposition of the physics of the process. Can you give an idea of the scale of the images, or at least the objective powers used?
Thanks for sharing.
Tom W.
Thanks for sharing.
Tom W.
- Wed Oct 02, 2019 8:31 pm
- Forum: Beginner's corner
- Topic: Mounting media
- Replies: 5
- Views: 673
Re: Mounting media
Hi Aslan You might take a look Electron Microscopy Sciences. In particular https://www.emsdiasum.com/microscopy/products/histology/mounting_media.aspx#17997 . They are based in the states, but I have ordered from them in the past and received very good service. Many of their products, though apparen...
- Wed Oct 02, 2019 8:13 pm
- Forum: Off Topic
- Topic: The Explorer's Guide to Biology
- Replies: 2
- Views: 1669
Re: The Explorer's Guide to Biology
Looks like this might be very interesting. Thank you for the link.
Tom W.
Tom W.
- Sun Sep 29, 2019 2:51 pm
- Forum: Pictures and Videos
- Topic: Colurella sp
- Replies: 14
- Views: 960
Re: Colurella sp
I think there's a lesson here for all of us 75. It seems that with only one microscope, and only one objective, and one or two sampling locations, it's possible to keep turning out remarkable images! I appreciate it's not quite as simple as that of course, but it does cause one to pause and think a ...
- Sun Sep 08, 2019 3:13 pm
- Forum: Home-made microscope adaptations
- Topic: EOS 4000D modification
- Replies: 5
- Views: 799
Re: EOS 4000D modification
Thanks for that Dave. I was about to make a dummy battery and install it using a similar slot for the cable, something I've previously done on a camcorder for my son. I didn't realize that dummy batteries were commercially available but will certainly make use of one now. Tom W. This is the one I b...
- Sun Sep 08, 2019 11:54 am
- Forum: Home-made microscope adaptations
- Topic: EOS 4000D modification
- Replies: 5
- Views: 799
Re: EOS 4000D modification
Thanks for that Dave. I was about to make a dummy battery and install it using a similar slot for the cable, something I've previously done on a camcorder for my son. I didn't realize that dummy batteries were commercially available but will certainly make use of one now.
Tom W.
Tom W.
- Fri Sep 06, 2019 10:19 pm
- Forum: Pictures and Videos
- Topic: Jellyfish gravity sensing organs
- Replies: 7
- Views: 844
Re: Jellyfish gravity sensing organs
Very informative Wes, and excellent images. Thank you.
Tom W.
Tom W.
- Thu Sep 05, 2019 11:23 am
- Forum: Pictures and Videos
- Topic: Rotifer and some algae
- Replies: 12
- Views: 1218
Re: Rotifer and some algae
Thank you! Well, the body was still, but the head moved. Therefore the head is barely visible except for the eye and the jaws. These I managed to get sharp in a couple of frames: The rest of the head not. If I plan for shooting rotifers, I use glutaraldehyde before I put on the cover glass. With ri...
- Thu Sep 05, 2019 1:08 am
- Forum: Pictures and Videos
- Topic: Marine worm (possibly Platynereis sp)
- Replies: 5
- Views: 566
Re: Marine worm (possibly Platynereis sp)
I agree with mintakax, interesting looking worm. So many interesting creatures in the sea. I grew up by the sea, but now live by the great lakes. They too are impressive, but I still miss the sea.
Tom W.
Tom W.
- Thu Sep 05, 2019 1:03 am
- Forum: Pictures and Videos
- Topic: Rotifer and some algae
- Replies: 12
- Views: 1218
Re: Rotifer and some algae
Wonderful images hkv. Thanks for sharing.
I'm intrigued by the rotifer image in that, despite stacking and stitching, there is very little evidence of movement. Is there a secret you're willing to share??
Tom W.
I'm intrigued by the rotifer image in that, despite stacking and stitching, there is very little evidence of movement. Is there a secret you're willing to share??
Tom W.
- Wed Aug 28, 2019 11:29 am
- Forum: Resources (online, books etc.)
- Topic: Protocols in Protozoology
- Replies: 3
- Views: 1062
Re: Protocols in Protozoology
Very interesting link Wes. Bedtime reading for months! Thank you.
Tom W.
Tom W.
- Mon Aug 26, 2019 10:53 pm
- Forum: Pictures and Videos
- Topic: Short Hand-Sectioning Demo with video
- Replies: 2
- Views: 885
Re: Short Hand-Sectioning Demo with video
Excellent John. Thank you.
Tom W.
Tom W.
- Mon Aug 26, 2019 1:35 am
- Forum: Pictures and Videos
- Topic: Pollen. There's lots of it about.
- Replies: 99
- Views: 30558
Re: Pollen. There's lots of it about.
I heartily agree with Bob. The collage is very attractive and informative. In fact if all three images in the collage above were of the same plant it would be perfect, and could even be extended, within limits, to include other details. As for the unknown, I've no idea, except that it definitely loo...
- Sun Aug 25, 2019 5:56 pm
- Forum: Pictures and Videos
- Topic: Pollen. There's lots of it about.
- Replies: 99
- Views: 30558
Re: Pollen. There's lots of it about.
Sorry Alan. Pictures failed to show up again (for me at least).
Tom W.
Tom W.
- Sat Aug 24, 2019 6:32 pm
- Forum: Home-made microscope adaptations
- Topic: Home made (Very Basic) Microtome
- Replies: 42
- Views: 4589
Re: Home made (Very Basic) Microtome
Good job all round Alan! Pretty soon John B is going to have to look to his laurels
Tom W.

Tom W.
- Tue Aug 20, 2019 2:16 pm
- Forum: Home-made microscope adaptations
- Topic: Home made (Very Basic) Microtome
- Replies: 42
- Views: 4589
Re: Home made (Very Basic) Microtome
Back when I was building and flying F1D Indoor Free Flight, I learned to dissect Gillette Mach 3 razor heads for the individual blades, which I super-glued to stick handles, to cut microfilm for wings and tail surfaces. Tiny little things are a bit of trick to handle, but even sharper yet. Nice to ...