MicrobeHunter.com Microscopy Forum

Awesome shots Wes!
tgss

Very nicely done Bob. I'll definitely be following you along on this journey.
Tom W.

Caught up with this one rather late. Amazing detail and contrast and beautifully sharp. Very well done - again.
Tom W.

Not a diatomist by any means, but based on many other images I've seen I'd say the COL is definitely an improvement.
Tom W.

Very nice 75RR. I note that your 63x doesn't seem to be wearing out with use - still producing just as good images as ever! The lower image in particular is very good.
Tom W.

+1 on the Ortho-Illuminators - very, very sexy!
Tom W

Thanks for the update on Part 4 of the series Kurt. I have the other three, but wasn't aware that Tony had released a fourth. As you stated, the books are a terrific source of thoroughly practical ideas and procedures, with attractive and informative illustrations. Tony is a bit of a Vickers M41 fan...

It's apparently available on Amazon Prime video, to rent - $4.99, or buy - $5.99, if that helps anyone.

Tom W.

Hi 75RR I'm beginning to feel guilty about hijacking Radazz's post with this but... I agree that you need to know what the pixel size really is in order to compare it with what is needed. I guess, for me at least, the discussion boils down to the recognition that, in order to figure out what pixel s...

Hi 75RR I think the misunderstanding is because I used an unfortunate term, pixel density, since it implies pixels/mm^2, or some such, whereas I was using it the sense of "number of pixels/sensor". That's because I'm an idiot. :oops: The intent was to get around the issue of different sized sensors ...

The pixel size should be 3 or 4 times smaller that the projected size of the object you are trying to capture. See link and the extract below: https://www.lumenera.com/blog/understanding-resolution-in-digital-microscopy please note that when they say: " largest possible pixel size required to fully...

I've been a little perplexed while following this thread. The idea that resolution depends primarily on pixel size is the idea that's causing me problems. I admit that "primarily" has not been explicitly stated, but seems to be implicit in the arguments. If carried to extremes this would imply that ...

Well... I'm gobsmacked! I couldn't understand what was going on when I read billbilt's posts - thought there must have been some kind of malfunction on the forum, until I read the following posts and realised it was for real. Very, very disappointed - really undermines the forum and leaves a bad tas...

Very unfortunate that this instrument was trashed Piter_. I wish you every success in restoring it to fully working condition.
Tom W.

What mrsonchus said .

Tom W.

I used a 10x objective, 10x eyepiece and 1.6x optovar magnification, both images were cropped from stacked photos. Later I can take some images of a stage micrometer and calculate the number of pixels per graduation to generate a scale bar. Thank you, Wes, for the clarification. No need to generate...

Fascinating stuff Wes, excellent images and a very clear exposition of the physics of the process. Can you give an idea of the scale of the images, or at least the objective powers used?
Thanks for sharing.
Tom W.

Hi Aslan You might take a look Electron Microscopy Sciences. In particular https://www.emsdiasum.com/microscopy/products/histology/mounting_media.aspx#17997 . They are based in the states, but I have ordered from them in the past and received very good service. Many of their products, though apparen...

Looks like this might be very interesting. Thank you for the link.
Tom W.

I think there's a lesson here for all of us 75. It seems that with only one microscope, and only one objective, and one or two sampling locations, it's possible to keep turning out remarkable images! I appreciate it's not quite as simple as that of course, but it does cause one to pause and think a ...

Thanks for that Dave. I was about to make a dummy battery and install it using a similar slot for the cable, something I've previously done on a camcorder for my son. I didn't realize that dummy batteries were commercially available but will certainly make use of one now. Tom W. This is the one I b...

Thanks for that Dave. I was about to make a dummy battery and install it using a similar slot for the cable, something I've previously done on a camcorder for my son. I didn't realize that dummy batteries were commercially available but will certainly make use of one now.
Tom W.

Very informative Wes, and excellent images. Thank you.
Tom W.

Thank you! Well, the body was still, but the head moved. Therefore the head is barely visible except for the eye and the jaws. These I managed to get sharp in a couple of frames: The rest of the head not. If I plan for shooting rotifers, I use glutaraldehyde before I put on the cover glass. With ri...

I agree with mintakax, interesting looking worm. So many interesting creatures in the sea. I grew up by the sea, but now live by the great lakes. They too are impressive, but I still miss the sea.
Tom W.

Wonderful images hkv. Thanks for sharing.
I'm intrigued by the rotifer image in that, despite stacking and stitching, there is very little evidence of movement. Is there a secret you're willing to share??
Tom W.

Very interesting link Wes. Bedtime reading for months! Thank you.
Tom W.

Excellent John. Thank you.
Tom W.

I heartily agree with Bob. The collage is very attractive and informative. In fact if all three images in the collage above were of the same plant it would be perfect, and could even be extended, within limits, to include other details. As for the unknown, I've no idea, except that it definitely loo...

Sorry Alan. Pictures failed to show up again (for me at least).
Tom W.