MicrobeHunter.com Microscopy Forum

Great, Bob.
The 40X/0.75 Ph2 Neofluoar is great when free of delamination.

...Sadly I have today discovered PWM generated strobe lines in a video I was editing, they are fine but noticeable. My initial testing was obviously not thorough enough, my apologies. I have edited the post to reflect this, back to the bench power supply (and the fan noise) for the time being until...

Very charming photo !!

What illumination was it - phase ?

Is that an ARRANGED slide ? they are so symmetrically laid.

Wow, what a magnificent project and results! 1. What is wrong with the original halogen lamp ? it seems to be too far from the stage to cause unwanted heating of slides ? 2. About the objectives: I recognize Zeiss West Neofluar Ph2 16X/0.40 (I own a similar one), Zeiss Jena objective and others. You...

I wanted to ask you to help me again with your suggestions. As I have no experience with reflected light microscopes, I have no reference to compare with. I notice that this microscope (in reflected light) produces images that are totally lacking in contrast, flat and of very poor quality...complet...

I was stunned by the image quality of the images submitted. I have an Olympus microscope and was wondering how these photographers were able to obtain such amazing clarity in their images. Is there anyway to find out what equipment these microscopists used ? I have tried many different ways to obta...

"Fluorescence" objectives are sometimes made of special glass that transmits much UV. If your excitation wavelength is above 400nm, ordinary objectives are just fine. The important feature is the NA, since the objective needs to gather as much light as possible, AND the objective serves as condenser...

I have both a micrometer and a caliper but it is true that a caliper can do both jobs at a pinch, particularly if it is possible to see the difference between two measurements even if it lacks a vernier. I think that for measuring thin items, a micrometer is more reliable simply because the contact...

I support MicroBob's advice above. In addition, if you happen to find an old 100X/1.25 objective, in which the front lens is very small, may want to try it as is, without any funnel, for DF. The background will not be pitch black but fairly dark. Based on personal experience.

Great find and very nice images! it is a diatom, sure.

With the riddle solved, we can turn all our time and energy to free the globe of the strangle covid-19...

So guys already added air pressure automation to the Orthoplan, but I am not sure they included the turret. Journal of Immunological Methods, 7 (1975) 255--270 © North-Holland Publishing Company, Amsterdam -- Printed in The Netherlands INEXPENSIVE AUTOMATION OF THE LEITZ ORTHOPLAN MICROFLUOROMETER U...

The objectives appear to be types of high magnification no cover objectives. One visible says 160X. Where the hose is connected , there is a large central screw supporting what looks to be the housing of some rotary device. Yes, the fact (??) that there is just one hose votes against circulation. C...

https://en.wikipedia.org/wiki/Diatom#/media/File:Paralia_sulcata_diatom.tif Agree with all above responses, so well expressed (mrsonchus) and may I add a small personal opinion: The beauty of a light-microscope image of clean diatoms - or even better, a live diatom, on a clean background, as often ...

I cannot say about compatibility, but filter cubes have been made by third parties for the major brand microscopes. I would look in Edmund, Newport, Chroma, Omega catalogues.

Or just oil immersion on the press of a button! Bob, this may be a lead. For inverted scopes and long-term observations - e.g. when one studies live cells in a stage incubator - immersion oil is a problem when needed. Because the oil slowly drips down and disappears from the space between the slide...

I would guess that the hose is there as part of a heating system. Perhaps the owner kept the nosepiece at a higher temperature than room temperature, maybe for work at 37C (live tissue/cells). Perhaps warm water was flowing or circulated (is there another hose end there ?). The water might have bee...

I would guess that the hose is there as part of a heating system. Perhaps the owner kept the nosepiece at a higher temperature than room temperature, maybe for work at 37C (live tissue/cells). Perhaps warm water was flowing or circulated (is there another hose end there ?). The water might have been...

Please note, a really efficient heat sink is important for a 10W LED. Otherwise the microscope stand - and worse, the optics - might get damaged.
I presume the dimmer is a 600Hz PWM, not 600KHz ?
A low frequency of the PWM might cause banding in the camera images.
Stay safe and sound.

MichaelG. wrote: ↑

Mon Mar 23, 2020 10:24 am

I am amused [and mildly irritated], to see that apochronaut’s jig has, when quoted, magically produced a hyperlink to an advertisement.

... We live in mysterious times.

MichaelG.

The link appears in the response before the quote

75RR wrote: ↑

Sun Mar 22, 2020 7:46 pm

Girdle view. Upper and Lower valve overlap can be distinguished.

Fantastic image ! they are so nicely distinguishable !
Do you think that this type of overlap can be identified with phase contrast ? (of any diatom, not necessarily this one)

I was wondering if anyone could post comparison images taken using an Olympus BH2/BHS and a modern microscope. I would like to compare the quality of the images produced with both using the best hardware for each, for example using an Splan Apo objective on the Olympus versus the best on a more mod...

Great photos, Bob.
Polarized light helps to differentiate some fibers from others.
Specimen no 3 (toilet paper) should now be considered very rare and precious under the shortage imposed by the crisis ! to be kept in a locked steel safe !

MichaelG. wrote: ↑

Sat Mar 21, 2020 7:22 pm

I have located the paper about the Spider web droplets:
https://www.pnas.org/content/pnas/early ... 3.full.pdf

MichaelG.

Michael,
Thanks for the link.
BTW - in some webs that I inspected, there were no droplets at all. Perhaps droplets are specific to the species.

Somebody interested in working on a common topic? Hi Bob, Years ago I posted some phase contrast and darkfield photos of spider's webs on the forum. I collected them from the hedges around. By simply touching a clean slide to a web (of a tiny spider, who fled away) the fibres easily stick onto the ...

Very beautiful !

Superb !

Larry060 wrote: ↑

Sun Mar 15, 2020 1:49 pm

I will search for a trinocular head.

An excellent choice.

...Some GFLs had a removable nosepiece, most had it not from what I saw in Germany. I have one GFL with a removable 5-position nosepiece and one GFL with a fixed 4-position nosepiece. They both rotate smoothly (for years after lubricating with a tiny drop of sewing-machine oil). The removable nosep...

Attractive image !