Search found 2367 matches

by Hobbyst46
Tue Mar 31, 2020 8:29 pm
Forum: Pictures and Videos
Topic: Twi ciliates from my plancton aquarium in phase contrast
Replies: 2
Views: 22

Re: Twi ciliates from my plancton aquarium in phase contrast

Great, Bob.
The 40X/0.75 Ph2 Neofluoar is great when free of delamination.
by Hobbyst46
Tue Mar 31, 2020 7:36 pm
Forum: Microscopes and optics
Topic: Vickers M17 refurb' & modification
Replies: 22
Views: 175

Re: Vickers M17 refurb' & modification

...Sadly I have today discovered PWM generated strobe lines in a video I was editing, they are fine but noticeable. My initial testing was obviously not thorough enough, my apologies. I have edited the post to reflect this, back to the bench power supply (and the fan noise) for the time being until...
by Hobbyst46
Mon Mar 30, 2020 9:38 pm
Forum: Pictures and Videos
Topic: Assorted algae
Replies: 2
Views: 58

Re: Assorted algae

Very charming photo !!

What illumination was it - phase ?

Is that an ARRANGED slide ? they are so symmetrically laid.
by Hobbyst46
Mon Mar 30, 2020 8:56 pm
Forum: Microscopes and optics
Topic: Vickers M17 refurb' & modification
Replies: 22
Views: 175

Re: Vickers M17 refurb' & modification

Wow, what a magnificent project and results! 1. What is wrong with the original halogen lamp ? it seems to be too far from the stage to cause unwanted heating of slides ? 2. About the objectives: I recognize Zeiss West Neofluar Ph2 16X/0.40 (I own a similar one), Zeiss Jena objective and others. You...
by Hobbyst46
Mon Mar 30, 2020 7:17 pm
Forum: Microscopes and optics
Topic: Mitutoyo finescope fs100
Replies: 46
Views: 6835

Re: Mitutoyo finescope fs100

I wanted to ask you to help me again with your suggestions. As I have no experience with reflected light microscopes, I have no reference to compare with. I notice that this microscope (in reflected light) produces images that are totally lacking in contrast, flat and of very poor quality...complet...
by Hobbyst46
Sun Mar 29, 2020 6:54 pm
Forum: Pictures and Videos
Topic: Olympus image of the year competition
Replies: 4
Views: 117

Re: Olympus image of the year competition

I was stunned by the image quality of the images submitted. I have an Olympus microscope and was wondering how these photographers were able to obtain such amazing clarity in their images. Is there anyway to find out what equipment these microscopists used ? I have tried many different ways to obta...
by Hobbyst46
Sun Mar 29, 2020 10:20 am
Forum: For forum members who want to buy and sell equipment
Topic: Wanted: Leitz 160 or 170mm Objectives, Plan or Better
Replies: 3
Views: 95

Re: Wanted: Leitz 160 or 170mm Objectives, Plan or Better

"Fluorescence" objectives are sometimes made of special glass that transmits much UV. If your excitation wavelength is above 400nm, ordinary objectives are just fine. The important feature is the NA, since the objective needs to gather as much light as possible, AND the objective serves as condenser...
by Hobbyst46
Sun Mar 29, 2020 10:14 am
Forum: Microscopes and optics
Topic: 40x SPlan woes
Replies: 13
Views: 310

Re: 40x SPlan woes

I have both a micrometer and a caliper but it is true that a caliper can do both jobs at a pinch, particularly if it is possible to see the difference between two measurements even if it lacks a vernier. I think that for measuring thin items, a micrometer is more reliable simply because the contact...
by Hobbyst46
Fri Mar 27, 2020 9:01 am
Forum: Illumination Techniques
Topic: 100x objective funnel stop
Replies: 7
Views: 111

Re: 100x objective funnel stop

I support MicroBob's advice above. In addition, if you happen to find an old 100X/1.25 objective, in which the front lens is very small, may want to try it as is, without any funnel, for DF. The background will not be pitch black but fairly dark. Based on personal experience.
by Hobbyst46
Wed Mar 25, 2020 9:28 pm
Forum: Pictures and Videos
Topic: A close-up of a broken diatom?
Replies: 9
Views: 156

Re: A close-up of a broken diatom?

Great find and very nice images! it is a diatom, sure.
by Hobbyst46
Wed Mar 25, 2020 9:02 pm
Forum: Microscopes and optics
Topic: Strange apparatus on Leitz Orthoplan nosepiece?
Replies: 28
Views: 431

Re: Strange apparatus on Leitz Orthoplan nosepiece?

With the riddle solved, we can turn all our time and energy to free the globe of the strangle covid-19... :roll:
by Hobbyst46
Tue Mar 24, 2020 2:49 pm
Forum: Microscopes and optics
Topic: Strange apparatus on Leitz Orthoplan nosepiece?
Replies: 28
Views: 431

Re: Strange apparatus on Leitz Orthoplan nosepiece?

So guys already added air pressure automation to the Orthoplan, but I am not sure they included the turret. Journal of Immunological Methods, 7 (1975) 255--270 © North-Holland Publishing Company, Amsterdam -- Printed in The Netherlands INEXPENSIVE AUTOMATION OF THE LEITZ ORTHOPLAN MICROFLUOROMETER U...
by Hobbyst46
Tue Mar 24, 2020 12:54 pm
Forum: Microscopes and optics
Topic: Strange apparatus on Leitz Orthoplan nosepiece?
Replies: 28
Views: 431

Re: Strange apparatus on Leitz Orthoplan nosepiece?

The objectives appear to be types of high magnification no cover objectives. One visible says 160X. Where the hose is connected , there is a large central screw supporting what looks to be the housing of some rotary device. Yes, the fact (??) that there is just one hose votes against circulation. C...
by Hobbyst46
Tue Mar 24, 2020 12:35 pm
Forum: Miscellaneous
Topic: Large SEM of Paralia sulcata
Replies: 7
Views: 131

Re: Large SEM of Paralia sulcata

https://en.wikipedia.org/wiki/Diatom#/media/File:Paralia_sulcata_diatom.tif Agree with all above responses, so well expressed (mrsonchus) and may I add a small personal opinion: The beauty of a light-microscope image of clean diatoms - or even better, a live diatom, on a clean background, as often ...
by Hobbyst46
Tue Mar 24, 2020 12:05 pm
Forum: Microscopes and optics
Topic: Leitz Ploemopak fluorescence filter cubes, compatibility?
Replies: 1
Views: 64

Re: Leitz Ploemopak fluorescence filter cubes, compatibility?

I cannot say about compatibility, but filter cubes have been made by third parties for the major brand microscopes. I would look in Edmund, Newport, Chroma, Omega catalogues.
by Hobbyst46
Tue Mar 24, 2020 12:00 pm
Forum: Microscopes and optics
Topic: Strange apparatus on Leitz Orthoplan nosepiece?
Replies: 28
Views: 431

Re: Strange apparatus on Leitz Orthoplan nosepiece?

Or just oil immersion on the press of a button! Bob, this may be a lead. For inverted scopes and long-term observations - e.g. when one studies live cells in a stage incubator - immersion oil is a problem when needed. Because the oil slowly drips down and disappears from the space between the slide...
by Hobbyst46
Mon Mar 23, 2020 10:38 pm
Forum: Microscopes and optics
Topic: Strange apparatus on Leitz Orthoplan nosepiece?
Replies: 28
Views: 431

Re: Strange apparatus on Leitz Orthoplan nosepiece?

I would guess that the hose is there as part of a heating system. Perhaps the owner kept the nosepiece at a higher temperature than room temperature, maybe for work at 37C (live tissue/cells). Perhaps warm water was flowing or circulated (is there another hose end there ?). The water might have bee...
by Hobbyst46
Mon Mar 23, 2020 9:53 pm
Forum: Microscopes and optics
Topic: Strange apparatus on Leitz Orthoplan nosepiece?
Replies: 28
Views: 431

Re: Strange apparatus on Leitz Orthoplan nosepiece?

I would guess that the hose is there as part of a heating system. Perhaps the owner kept the nosepiece at a higher temperature than room temperature, maybe for work at 37C (live tissue/cells). Perhaps warm water was flowing or circulated (is there another hose end there ?). The water might have been...
by Hobbyst46
Mon Mar 23, 2020 4:42 pm
Forum: Home-made microscope adaptations
Topic: Yet another LED retrofit topic
Replies: 5
Views: 177

Re: Yet another LED retrofit topic

Please note, a really efficient heat sink is important for a 10W LED. Otherwise the microscope stand - and worse, the optics - might get damaged.
I presume the dimmer is a 600Hz PWM, not 600KHz ?
A low frequency of the PWM might cause banding in the camera images.
Stay safe and sound.
by Hobbyst46
Mon Mar 23, 2020 4:21 pm
Forum: For forum members who want to buy and sell equipment
Topic: Compound microscope recommendations?
Replies: 16
Views: 1769

Re: Compound microscope recommendations?

MichaelG. wrote:
Mon Mar 23, 2020 10:24 am
I am amused [and mildly irritated], to see that apochronaut’s jig has, when quoted, magically produced a hyperlink to an advertisement.

... We live in mysterious times.

MichaelG.
The link appears in the response before the quote
by Hobbyst46
Sun Mar 22, 2020 9:53 pm
Forum: Pictures and Videos
Topic: Trachyneis aspera (another)
Replies: 17
Views: 362

Re: Trachyneis aspera (another)

75RR wrote:
Sun Mar 22, 2020 7:46 pm
Girdle view. Upper and Lower valve overlap can be distinguished.
Fantastic image ! they are so nicely distinguishable !
Do you think that this type of overlap can be identified with phase contrast ? (of any diatom, not necessarily this one)
by Hobbyst46
Sat Mar 21, 2020 11:22 pm
Forum: Camera systems and imaging
Topic: Olympus BHS vs modern microscope
Replies: 8
Views: 344

Re: Olympus BHS vs modern microscope

I was wondering if anyone could post comparison images taken using an Olympus BH2/BHS and a modern microscope. I would like to compare the quality of the images produced with both using the best hardware for each, for example using an Splan Apo objective on the Olympus versus the best on a more mod...
by Hobbyst46
Sat Mar 21, 2020 8:51 pm
Forum: Miscellaneous
Topic: "Fibres" common topic? Did you make interesting observations or photos?
Replies: 17
Views: 478

Re: "Fibres" common topic? Did you make interesting observations or photos?

Great photos, Bob.
Polarized light helps to differentiate some fibers from others.
Specimen no 3 (toilet paper) should now be considered very rare and precious under the shortage imposed by the crisis ! to be kept in a locked steel safe ! :lol:
by Hobbyst46
Sat Mar 21, 2020 8:46 pm
Forum: Miscellaneous
Topic: "Fibres" common topic? Did you make interesting observations or photos?
Replies: 17
Views: 478

Re: "Fibres" common topic? Did you make interesting observations or photos?

MichaelG. wrote:
Sat Mar 21, 2020 7:22 pm
I have located the paper about the Spider web droplets:
https://www.pnas.org/content/pnas/early ... 3.full.pdf

MichaelG.
Michael,
Thanks for the link.
BTW - in some webs that I inspected, there were no droplets at all. Perhaps droplets are specific to the species.
by Hobbyst46
Fri Mar 20, 2020 9:30 pm
Forum: Miscellaneous
Topic: "Fibres" common topic? Did you make interesting observations or photos?
Replies: 17
Views: 478

Re: "Fibres" common topic? Did you make interesting observations or photos?

Somebody interested in working on a common topic? Hi Bob, Years ago I posted some phase contrast and darkfield photos of spider's webs on the forum. I collected them from the hedges around. By simply touching a clean slide to a web (of a tiny spider, who fled away) the fibres easily stick onto the ...
by Hobbyst46
Wed Mar 18, 2020 12:26 pm
Forum: Pictures and Videos
Topic: two more marine diatoms
Replies: 6
Views: 744

Re: two more marine diatoms

Very beautiful !
by Hobbyst46
Wed Mar 18, 2020 12:24 pm
Forum: Pictures and Videos
Topic: another Pleurosigma
Replies: 4
Views: 450

Re: another Pleurosigma

Superb !
by Hobbyst46
Sun Mar 15, 2020 2:13 pm
Forum: Beginner's corner
Topic: My New Microscope and need some help
Replies: 21
Views: 6001

Re: My New Microscope and need some help

Larry060 wrote:
Sun Mar 15, 2020 1:49 pm
I will search for a trinocular head.
An excellent choice.
by Hobbyst46
Sun Mar 15, 2020 2:08 pm
Forum: Microscopes and optics
Topic: Zeiss Experts: What Model is This?
Replies: 26
Views: 3017

Re: Zeiss Experts: What Model is This?

...Some GFLs had a removable nosepiece, most had it not from what I saw in Germany. I have one GFL with a removable 5-position nosepiece and one GFL with a fixed 4-position nosepiece. They both rotate smoothly (for years after lubricating with a tiny drop of sewing-machine oil). The removable nosep...
by Hobbyst46
Sun Mar 15, 2020 6:40 am
Forum: Pictures and Videos
Topic: Latest image
Replies: 5
Views: 1529

Re: Latest image

Attractive image !