MicrobeHunter.com Microscopy Forum

...I could probably do with a better camera. The one I have is ok-ish (It's a Touptek 5M with Aptina sensor and 2.2um pixels) but tends to be a bit grainy. If the camera is an eyepiece camera, so is being used without eyepiece, the Nikon CF objective is expected to perform very well, since it is in...

Best would be to use a kind of "mash" or specimen with lines for a comparison. Example ist here: http://www.microbehunter.com/microscopy-forum/viewtopic.php?f=24&t=5930&start=60#p71902 But this of coarse raises the question where to get such. But maybe somebody has another idea? Micrometer slides a...

Thanks 75RR for the catalogue !!

...Using the 20x, doing the projection on to a piece of paper gives a changing circle of light which comes into focus at a point above the head - I'm guessing at ~160mm! Same with 10x finite objective. So I really need better quality finite objectives from somewhere... Although the reproducibility ...

...At the end of the day, there is really just the objective lens and the camera, from an imaging point of view. It ain't necessarily so. On the BX50 imaging system showed above, I believe that it was the objective, tube lens and camera; if one had used the dedicated Olympus camera like the DP70, D...

Do you know how big the field is that your camera captures? Did you cover the paper with water or oil and a cover slip? I'm unsure how to check the field size. I used an afocal setup with a 10x eyepiece and my Canon M50 with an 18-45mm lens. I wanted to leave the entirety of the eyepiece visible so...

75RR, thanks for the idea. Done.

I've never quite understood exactly what a tube lens does in terms of aberration and maybe also colour correction? Thanks for any expertise/advice :) Here is a compact description of the difference : "Handbook of Biological Confocal Microscopy, Volume 236" edited by James Pawley, pages 156-157 (vie...

I own an AmScope T490B - Which comes with 4x, 10x, 40x, and 100x (oil) Non-Plan objectives (160mm). I figured I should upgrade to their Plan objectives, and wanted to do a comparison in case anyone else was also looking to buy the same objectives. I took pictures of a printed image with a coverglas...

MichaelG. wrote: ↑

Thu Jul 09, 2020 12:52 pm

75RR wrote: ↑

Thu Jul 09, 2020 9:55 am

To save interested parties having to type out the sentence in a translator, here it is:
[…]

...There was a wise old saying amongst Scientific Instrument Makers :
If you can’t make it right, make it adjustable.

Try the water from a flower vase after the flowers we're in for a few day’s. Or squeeze the liquid out of a bit of water soaked moss if you can find some (with luck you find some water bears). Weird the cultures from (dried) lettuce, hay, a corn of grain, tiny piece of cheese etc. did not work out....

Here is an example of an Olympus BH-2. With phase contrast. Just picked it out of eBay. I am not related to the seller in any way. https://www.ebay.com/itm/Olympus-Trinocular-BH2-BHTU-Phase-Contrast-Microscope/203040702828?hash=item2f462b416c:g:IMwAAOSwpq1fAnd7 With phase condenser and two SPlans (I...

This post, about DIY incident LED illumination of objects of similar size as you mention, may be relevant
viewtopic.php?f=6&t=7504&p=66513&hilit=strip#p66513

Welcome Louise, Here is a fairly recent post that might be relevant http://www.microbehunter.com/microscopy-forum/viewtopic.php?t=7666 BTW, even (or especially...) for the big 4 modern microscopes, which are all infinity-corrected, objectives are not interchangeable among brands. And here a very rec...

I am not as experienced as you all and I am wondering if, or why, I should wipe dry ? I only ever stand my reused washed** slides and cover slips on edge over absorbent paper to air dry. What am I missing, what should I be looking for ? I assumed that by wiping with something I was in danger of int...

I have to apologize for my previous response - I totally misunderstood the question. I thought you were seeking a liquid solution of dye or something else to pour on the cover slip and make a contrast area, a target for focusing , instead of focusing on fingermarks and such. My answer was formulated...

very nice !

Hi Doron, thank you for the explanation! Building this ingenious setup must have taken quite some time. And there are probably few other designs that include a micrometer as well as Lego bricks! :lol: For my attempts at diatom arranging I used a simpler setup but was not that happy with it. It made...

No need to pour dirt onto the slide. The default status of everything on Earth is dirty - cleaning is working against laws of Physics. Here is a practical suggestion: always start with a low magnification - best of all, the 10X. Bring the fine focus knob to its mid-range position. With the coarse fo...

As a temporary check, could put a tight-fitting o-ring on the male dovetail. They come in all string sizes. Some are made of strong polymers, though are less elastic than simple rubber so must be within a few millimeters of the final diameter.

Hi Doron, congratulations to this nice setup! For some samples it probably is much more worthwhile to pick good frustules out than to try to clean the sample to a high grade. Do you use a sticky substance on the tip of your fibre? Is the fibre attatched to the objective and moves with the focussing...

Thanks Charles.
I noticed that your diatoms, the ones that you pick under the stereo microscope view, are clearly visible - the contrast against the background is very good.
Is it "ordinary" transmitted illumination ? the original lamp of the microscope ?

Motic seem confused too. In the product description for the Panthera they state "full Kohler", but on the same page in the features list they say "fixed Kohler". I don't see a field diaphragm. . Curiouser and Curiouser : Have a look at p12 of this document [p13 of the PDF] https://genexpress.cl/wp-...

Charles: I tried to attach the glass fiber with sugar water (fructose syrup actually) but was too impatient to wait for drying, so I switched to nail polish.

(continued) the focus is not the best, sorry. When the fiber is raised, either it or the diatoms on the slide could be in focus - not both. In photos 2 and 4, the round diatom is carried at the tip of the fiber. I must say, for me, it was easier to find this fiber than to pull an elegant tapered tip...

Update: The "bristle" made of a toothbrush hair works, but is somewhat too thick. I sought a thinner bristle. And found a glass fiber. Not one that was pulled from a tube. Just a fiber. Glass fibers, in the form of glass wool, is a know heat isolator. So I just pulled a tiny bit out and managed to c...

1:0 Bob ! very charming !

I'm still not clear what is the difference between "fixed Köhler" and a microscope without Köhler illumination. Prefocused on the condenser front focal plane perhaps ... I think that the "fixed" in this context refers to the lamp only. The lamp has been optimized (within the cost limitations as set...

MichaelG. wrote: ↑

Wed Jul 01, 2020 8:30 am

It appears to be a ‘convenient approximation’ for routine [non-critical] use, David

This, from Zeiss, goes a little further towards explaining:
http://focuspi.com/Site/AXIO_SCOPE_A-1_ ... 0004_e.pdf]
[see pp7-9]

MichaelG.

Probably, it has no field diaphragm...

To me, it means that incineration is a rapid yes-or-no test, to verify that frustules of interest are in the collected sample - just what you are now enjoying on the lake ! One would think that viewing wet slides would provide that information, to genus level anyway. I wonder if it would be possibl...