MicrobeHunter.com Microscopy Forum

All my equipment is old Zeiss (west), originally all parts are designed to work together. Since you have the matching microscope, another related question, if you don't mind: Is there a lens (called the field lens or collimator by some manufacturers, not sure about Zeiss) between the condenser and ...

Hi Doron, I thought of a thermocouple as they are small. The more common PT or NTC sensors would be too big to fit next to the front lens. I don't know the electrical resistance of thermocouples, but a resistance measurement might help to narrow down the selection of opportunities. These could also...

It often happens with LED light. You might try to lower the exposure speed - expose for longer peridos. 1/15 s instead of 1/30 s, etc.

Interesting catch! With all the heating it would make sense to measure the temperature. Is it possible that the tiny elements next to the front lens are a thermocouple? They put out a tiny voltage, perhaps you can measure it. Bob Bob, indeed, that wiring in the plane of the front lens very likely l...

...correct immersion gap... This is basically why I was wondering. Practical setup issues aside, once one of these reflective darkfield condensers is set at the correct working distance in the microscope it was originally designed for, is the field diaphragm expected to be imaged in the object plan...

Heated by two gadgets. The top one - the brown tape - is a heating tape for use. Electrical. The collar with inlet and outlet tubing is likely a water circulation collar, to heat the nose of the objective. Was possibly used for the study of live cells at 37C; the cells were sitting in a petri dish o...

hi mrsonchus,
Is the camera not supported by Toupview ? or MICAM perhaps ?

A 100X1.25 SI objective like the one shown in the bottom photo must be in one of my drawers. If I can find it, attach to the DIN micoscope via a spacer ring to create some parfocality, and get some silicon oil, then it will be interesting to test. Viktor - which specimen do you plan to image with th...

Turning/milling is IMO a great skill, in which one can become an expert and produce highly precise items, depending on motivation and will to excel. Furthermore, turning and milling are creativity, and I believe they can become a hobby as well. Although 3D printing is here, making quality and precis...

Hello Everybody, I haven't gotten a microscope since I was about 10 years old....I'm now 72. That first microscope had no light, had little clips to hold the slides in place, (which constantly fell out), and rediculous lenses that produced very blurry views at higher power. So, here I am, consideri...

Sweet kid !!!
Could be a sales promotion poster for the BH2 (not BL2) microscope. Enjoy !

Thanks Michael and Viktor,
Incidentally, I am sure I have once seen an old 100X objective - almost certainly Olympus - that bore the mark "SI". In retrospect, might have been short for Silicone Immersion ?

Maybe not silicone immersion? https://en.wikipedia.org/wiki/Solid_immersion_lens https://www.twi-global.com/pdfs/equipment-factsheets/Automated-light-microscope-and-image-analyser.pdf Very interesting. Indeed SIL (solid imm.) yields super resolution; there is a lens that is made of high refractive ...

Tried to imitate your flashlight experiment on my Ultracondenser and did not find any similar effects to those on yours. Yes, I discovered delamination in both objectives and eyepieces from Zeiss. Not on condensers (two simple condensers and three phase contrast condensers, although I did not check ...

Hans, Here are posts about my experience with the Ultracondenser. On mine, as received, paint was flaking off but I managed to fix it. It works very well and produces excellent DF with 40X0.75, 40X1.0 (oil) and even a fairly nice DF (or at least oblique) with an old achromat 100X1.25 (oil) - see lin...

Simple inexpensive generic 5MP eyepiece cameras are usable on various microscopes. The only requirement is the inner diameter of the photo tube, which in most cases is 23.2 mm.
I have a 5MP USB eyepiece camera with which I recorded decent images from Zeiss, Olympus and Nikon microscopes.

A very slight (IMHO) advantage of the 40X UC objective. For darkfield, a lot of light is needed. A 30W halogen or 3W LED on the 310 model does not sound extremely bright. On the other hand, the Panthera specs boast full Kohler illumination. That would be an advantage. My own preference would be agai...

My occasional experience with low-density silicone oil (not for microscopy) is that it IS messy. On the other hand it is very stable, much more so than any natural oil. Will not deteriorate with time. And fairly expensive - since the smallest package is way larger to what we need for microscopy.

If anyone is interested, Oliver reviewed the Chinese dslr adapter a year ago: https://www.youtube.com/watch?v=Ju8rgeJr3bI Louise For an old finite optics microscopes in which spatial and color corrections are done in both the objective and eyepiece, I doubt that those DSLR adapters (when they inclu...

Out of curiosity, I spotted this ad "10pc R155zz (5/32" x 5/16" x 1/8") Metric Shielded Ball Bearing Bearings R155" https://www.ebay.com/itm/10pc-R155zz-5-32-x-5-16-x-1-8-Metric-Shielded-Ball-Bearing-Bearings-R155/153339567359?hash=item23b3c01cff:g:QcsAAOSw9N1Vt6iX the data sheet indicates Imperial ...

If the chief aim of the microscope is fluorescence, epi-fluorescence that is, the condenser is less important. High NA objectives on the other hand are very important for fluorescence. Low light calls for high NA. IMHO, given that the fluorescence is a very limited assortment of wavelengths, probabl...

There are high intensity commercial LED lamps for microscopy, including power supply and dimmer, by the name CoolLED. Pretty expensive. Can be attached to an Olympus BX microscope (and other microscopes), so I believe can be interfaced to a BH2 stand. One still needs the epi-illumination field aper...

There are high intensity commercial LED lamps for microscopy, including power supply and dimmer, by the name CoolLED. Pretty expensive. Can be attached to an Olympus BX microscope (and other microscopes), so I believe can be interfaced to a BH2 stand. One still needs the epi-illumination field apert...

I like the oblique illumination photos ! well done.

... 1. is it possible with this infinity optic to use say Olympus SPlan 160 mm tube objective with adapter? Will it be optically OK? I am afraid my comment about the RMS was irrelevant. Sorry. The BHM is an infinity corrected system, SPlans and Dplans are 160mm objectives, so incompatible. The comp...

1. NeoSplan objectives have 26mm threads, not 25mm. There must be sleeve adapters from 25 or 26mm to RMS. Non metallurgical SPlans and DPlans are RMS. 2. If the microscope has been used for metallurgy, where is the epi-illuminator. 3. A BH2 in good mechanical condition is a pleasure to have. 4. Obje...

Certainly even better images ! improved sharpness, higher resolution and less CA, supporting the hypothesis that the Naphrax slide is OK, and the images can be improved via the photographic gear. I think that the Canon 1100D altogether yields slightly better pictures than the Toupcam. Are you using ...

Nice choice, limonene as substitute for toluene. Likely, it will dissolve Naphrax well (my guess). Its disadvantage for Naphrax mounting is its high boiling point of 176C. Hence the slide must be heated long enough to remove the limonene, otherwise the RI will not reach 1.7. Of course heating the sl...

The Pleurax slide seems much better to me. My dslr pics always seem to come out not very clear - no sharp focus. I've been using a Chinese 'dslr adapter' for the trinocular attachment (which has an inbuilt lens) - not sure if the images through that are as good as they might be. Any thoughts, sugge...

...Nothing fancy, I just took some samples in small jars back in November. They sit on a window sill in my living room and in the (winter) light. But they are considerably warmer than outdoor ambient temperatures which have been sub zero here recently (brrr!). I've kept them topped up with boiled w...