Search found 2899 matches

by Hobbyst46
Wed Sep 30, 2020 3:16 pm
Forum: Resources (online, books etc.)
Topic: Looking for a Olympus Tokyo CK inverted Microscope Manual
Replies: 7
Views: 137

Re: Looking for a Olympus Tokyo CK inverted Microscope Manual

Some other forum members might know more about the CK model - have you seen this thread ?
viewtopic.php?t=6508
by Hobbyst46
Tue Sep 29, 2020 4:50 pm
Forum: Beginner's corner
Topic: Inexpensive complete Balplan on ebay.
Replies: 14
Views: 681

Re: Inexpensive complete Balplan on ebay.

I'll bet a lot of people think 2.5x isn't worth much, but I like it as it allows me to scan water for interesting things very quickly. Maybe I'd like the 4x as well. A 2.5X is very appropriate for larger specimens, for example foraminifera, moss leaves... There are even 1X objectives, and they are ...
by Hobbyst46
Tue Sep 29, 2020 6:44 am
Forum: My microscope
Topic: Cleaning mirrors in objectives of AO 570 stereoscope
Replies: 17
Views: 182

Re: Cleaning mirrors in objectives of AO 570 stereoscope

Enjoy ! AO 570 stereos are excellent. The 0.7-4.2X range is very useful.
by Hobbyst46
Mon Sep 28, 2020 4:44 pm
Forum: My microscope
Topic: Cleaning mirrors in objectives of AO 570 stereoscope
Replies: 17
Views: 182

Re: Cleaning mirrors in objectives of AO 570 stereoscope

From the description of the spots/staining, neither any solvent nor vinegar will help. Maybe just breathing on them to form a fog, then a quick bare touch with a thin Q-tip, will show if it is external or the coating is damaged. As others mention, the coating is liable to come off.
by Hobbyst46
Sat Sep 26, 2020 5:10 pm
Forum: Illumination Techniques
Topic: Stereo Microscope : Fluorescence Adapter
Replies: 51
Views: 11547

Re: Stereo Microscope : Fluorescence Adapter

Our paper describing the polarization and fluorescence modifications is now on-line: https://pubs.acs.org/doi/10.1021/acs.jchemed.0c00518 Supporting information is free to download (section III has more details of the optics). Message me if you want a copy of the main text Thanks for the article an...
by Hobbyst46
Sat Sep 26, 2020 1:23 pm
Forum: Pictures and Videos
Topic: Diatom 640X Oblique Illumination
Replies: 41
Views: 4736

Re: Diatom 640X Oblique Illumination

Not just fine - Charming !
Which objective, mrsonchus ?
by Hobbyst46
Thu Sep 24, 2020 8:28 am
Forum: Home-made microscope adaptations
Topic: Microstar IV Camera Adapter
Replies: 5
Views: 114

Re: Microstar IV Camera Adapter

Impressive project ! Hopefully images recorded with the setup will be posted ! Questions if I may. may add an external camera mount eventually to fix the camera even more firmly, but for now this will work quite nicely.. 1. I wonder about the feasibility of mechanical stabilization of the camera on ...
by Hobbyst46
Wed Sep 23, 2020 6:44 pm
Forum: Microscopes and optics
Topic: Thoughts on filling/reforming stage rack with epoxy?
Replies: 8
Views: 183

Re: Thoughts on filling/reforming stage rack with epoxy?

From the description I understand (incorrectly ?) that the tips of the teeth of the pinion do not touch the rack. If so, why should it affect functioning ? Both uniform coating of the rack with epoxy and spreading it with a powder to prevent unwanted adherence seem to me very challenging. If you go ...
by Hobbyst46
Mon Sep 21, 2020 3:08 pm
Forum: Pictures and Videos
Topic: Euplotes from the North Sea
Replies: 5
Views: 145

Re: Euplotes from the North Sea

Very clear and beautiful images.
by Hobbyst46
Sun Sep 20, 2020 3:31 pm
Forum: My microscope
Topic: Zeiss Opton Stativ W
Replies: 4
Views: 139

Re: Zeiss Opton Stativ W

Congratulations. A nice microscope. I like the stage. The Olympus DPlans are very good objectives. They will not be exactly parfocal with the Zeiss objectives so, it is more convenient to have a set of objectives from the same brand and line - if possible. Also, the Olympus and Zeiss objectives and ...
by Hobbyst46
Fri Sep 18, 2020 12:54 pm
Forum: Specimens, samples and slides
Topic: Bacterial behaviour.
Replies: 6
Views: 153

Re: Bacterial behaviour.

The gliding bacteria is most likely a species of flavobacteria. It moves at about 10 um/sec., probably faster mostly. It is hard to detect but there may be a type of corkscrew motion associated with the undulating or swimming motion. The thing is, due to it's small diameter of only about 2 um, unli...
by Hobbyst46
Fri Sep 18, 2020 10:25 am
Forum: Specimens, samples and slides
Topic: Bacterial behaviour.
Replies: 6
Views: 153

Re: Bacterial behaviour.

This seems to be a very interesting and intriguing topic ! Please elaborate on the dimensions. The 0.5 um to 10 or 60 um ? are all of them bacteria ? Perhaps one way to investigate them is under DF, if they scatter intensely, then time-lapse and/or video photography, then image analysis can tell abo...
by Hobbyst46
Sat Sep 12, 2020 10:12 am
Forum: Microscopes and optics
Topic: How clean is your solvent!
Replies: 8
Views: 718

Re: How clean is your solvent!

Very interesting ... ‘though the IPA performance is rather worrying ! I would not worry much about it, at least until someone shows, to which extent does the thin film left of the surface by the evaporating solvent (as done in the article!) interfere with optical microscopy. And, absolutely pure IP...
by Hobbyst46
Fri Sep 11, 2020 10:09 am
Forum: Microscopes and optics
Topic: Brass Rack and pinions designed to run dry?
Replies: 14
Views: 970

Re: Brass Rack and pinions designed to run dry?

Just realized that I live in Norway, whaling is still going on and the oil is on sale! No clock grade though, only "oil for dog fur" commercially available. They are discussing now if to reopen the hunt for porpoises... I'm sure in Japan they still make such lubricants. I found an article by Carl H...
by Hobbyst46
Thu Sep 10, 2020 8:03 pm
Forum: Specimens, samples and slides
Topic: Diatom selection experience
Replies: 38
Views: 4126

Re: Diatom selection experience

...mountant that doesn't need a bubbling curing action... I see bubbling mentioned a lot with these diatom mountants -- is it necessary for some physical/chemical reason, or is it just difficult to remove the solvent in a reasonable amount of time without bubbling? It is difficult to remove the sol...
by Hobbyst46
Thu Sep 10, 2020 1:34 pm
Forum: Specimens, samples and slides
Topic: Diatom selection experience
Replies: 38
Views: 4126

Re: Diatom selection experience

Hi Doron, nice to hear of you progress! According to your note 2: Michel Haak suggests polyacrylamide, not polyamide. I'm no chemist and don't know how far this is apart. http://www.diatoms.eu/de/node/77 The high curing temperature of Pleurax really narrows down the number of possible candidates fo...
by Hobbyst46
Thu Sep 10, 2020 8:53 am
Forum: Specimens, samples and slides
Topic: Diatom selection experience
Replies: 38
Views: 4126

Re: Diatom selection experience

[…] it appears that for some reason, wet diatoms attach better (to glass) than dry diatoms. Perhaps, this holds for gently cleaned frustules, that still contain trace amounts of protein or other organic residue that may function as adhesive. But it worked for me with chemically cleaned fossil diato...
by Hobbyst46
Wed Sep 09, 2020 7:48 pm
Forum: Specimens, samples and slides
Topic: Diatom selection experience
Replies: 38
Views: 4126

Re: Diatom selection experience

Thanks 75RR !
by Hobbyst46
Wed Sep 09, 2020 7:48 pm
Forum: Specimens, samples and slides
Topic: Diatom selection experience
Replies: 38
Views: 4126

Re: Diatom selection experience

Hi Doron, nice to hear of you progress! According to your note 2: Michel Haak suggests polyacrylamide, not polyamide. I'm no chemist and don't know how far this is apart. http://www.diatoms.eu/de/node/77 The high curing temperature of Pleurax really narrows down the number of possible candidates fo...
by Hobbyst46
Wed Sep 09, 2020 3:46 pm
Forum: Specimens, samples and slides
Topic: Diatom selection experience
Replies: 38
Views: 4126

Re: Diatom selection experience

Update: So after further experiments, I arrived at a practical (probably not ideal) method of isolation of cleaned diatom frustules from silt/debris/powder and mounting them in Pleurax. It works for isolation - not for arrangements. 1. Fish the cleaned diatoms out from the dry raw mixture (prepared ...
by Hobbyst46
Mon Sep 07, 2020 5:38 pm
Forum: Microscopes and optics
Topic: Advice new or refurbish my Zeiss?
Replies: 52
Views: 3517

Re: Advice new or refurbish my Zeiss?

...Hopefully an expert on Zeiss Axio will join and judge. >>> wrote them few days ago but no reply Just to clarify : I was referring to a microbe hunter forum member who has and/or uses Zeiss Axio, not a Zeiss salesperson or representative. Hopefully, with a little bit of luck, the trino head will ...
by Hobbyst46
Mon Sep 07, 2020 5:29 pm
Forum: Specimens, samples and slides
Topic: Where to buy slides and #1.5 coverslips
Replies: 6
Views: 745

Re: Where to buy slides and #1.5 coverslips

I found some slides on Amazon: Karter scientific slides with frosted end Levenhuk slides Are these any good? I found some #1.5 coverslips at Bioscience tools. “ High precision cover glasses, No 1.5, CSHP- Cover glasses for high performance microscopes, made of chemically resistant borosilicate glas...
by Hobbyst46
Sun Sep 06, 2020 1:54 pm
Forum: Microscopes and optics
Topic: Advice new or refurbish my Zeiss?
Replies: 52
Views: 3517

Re: Advice new or refurbish my Zeiss?

What would you pick ? The dovetail connectors of the three heads appear to be the same, or very similar to each other, and differ from the connector on the head of your present binocular. So, a dilema. Hopefully an expert on Zeiss Axio will join and judge. Or I would continue to investigate in Zeis...
by Hobbyst46
Sun Sep 06, 2020 6:54 am
Forum: Beginner's corner
Topic: Which to choose?
Replies: 7
Views: 456

Re: Which to choose?

...I'm looking to purchase my first microscope, mostly for my son to use in college, and for my wife and I to use for fun.... None of the used microscopes mentioned above is lightweight or otherwise adapted for frequent commuting (between home and college). Maybe the extent and scope of usage at ea...
by Hobbyst46
Sat Sep 05, 2020 8:35 pm
Forum: Pictures and Videos
Topic: Arcella discoides
Replies: 6
Views: 453

Re: Arcella discoides

Well done !
by Hobbyst46
Sat Sep 05, 2020 7:35 pm
Forum: Microscopes and optics
Topic: Microscope Maintenance
Replies: 25
Views: 1763

Re: Microscope Maintenance

... seems salt might have some effect as a buffer (???): Sodium chloride is formed from equally strong acid and base - hence does not buffer. If not to balance something in the solution, any idea why ROR put the bit of sodium chloride there? ROR has been used and recommended for years - must be som...
by Hobbyst46
Sat Sep 05, 2020 6:46 pm
Forum: Microscopes and optics
Topic: Microscope Maintenance
Replies: 25
Views: 1763

Re: Microscope Maintenance

I've had pretty good luck with "ROR" (Residual Oil Remover) lens cleaner. MSDS on it shows a tiny bit of ammonia (.775%), a fair small amount (just over 4%) of isopropyl alcohol, 9% soaps (maybe the same as above), distilled water, and a tiny bit of sodium chloride. The salt is a puzzle to me. Does...
by Hobbyst46
Sat Sep 05, 2020 3:23 pm
Forum: Microscopes and optics
Topic: Microscope Maintenance
Replies: 25
Views: 1763

Re: Microscope Maintenance

Hi together, the safety data sheets often are an interesting source of information. Apparently Lens Clens 1 consists of these dangerous components: Solvent content: Organic solvents:46.0 % Water:45.9 % VOC content:46.0 % Solids content:2.8 % CAS: 67-63-0RTECS: NT 8050000Isopropyl alcohol Flam. Liq....
by Hobbyst46
Sat Sep 05, 2020 1:43 pm
Forum: For forum members who want to buy and sell equipment
Topic: Considering upgrade of stereozoom 'scope
Replies: 28
Views: 1133

Re: Considering upgrade of stereozoom 'scope

...Very good VFM in my book, although it of course has it's limitations, the main one for me being the need to refocus after changing zoom aither way - as is also the case with the other model. The same limitation as on Olympus VMZ zoom. Focus needs tweaking when zoom is changed. I bought it used. ...
by Hobbyst46
Fri Sep 04, 2020 7:38 pm
Forum: Beginner's corner
Topic: Keeping a 60x objective from crashing
Replies: 10
Views: 1081

Re: Keeping a 60x objective from crashing

I recently replaced my 100x oil with a 60x dry objective. I'm having difficulty keeping it from crashing, even though it seems parfocal with the other objectives. As soon as I begin focusing with the fine focus knob, I see things in the wet mount start to shift around, which I assume means the obje...