MicrobeHunter.com Microscopy Forum

Too bad. I would first check continuity (resistance when power is off and wall plug cable is disconnected) between the PS outlet and the lamp socket. A bad connection is very often the problem. Screws in electrical connections often turn loose with time, during to heat effects. If the contacts are O...

Very nice catch, Bob! Impressive use of the dry 40X0.95. Please details about illumination, mounting media (or is it air?), darkfield condenser. I myself found some diatoms quite like your long one with rectangular "windows" in the shore mud of the Mediterranean Sea recently, but did not make perman...

Very attractive image !

May I note that there are slides with different depth wells. I think I have seen 0.2mm, 0.4mm, 0.6mm well slides, etc. Cannot reckon from which supplier.

... You can read the original (recent) research here: https://www.cell.com/current-biology/fulltext/S0960-9822(19)31431-9 personally I draw several practical conclusions from the original Jennings study and the recent research: 1) Jennings found "intelligence" not only in S. Roeseli but in other cr...

Sauerkraut wrote: ↑

Mon Dec 09, 2019 7:06 pm

...Chapter X (pg 170) describes the experiments with Stentor.

Thanks !

https://www.sciencenews.org/article/single-celled-protist-reacts-threats-surprisingly-complex-way... Applause for posting this link. Appears to be a very interesting scientific adventure! The link leads to a book by H. S. Jennings. Has anyone found the detailed description of the "messing" experime...

mrsonchus wrote: ↑

Sun Dec 08, 2019 7:55 pm

...I used a black card circle to get DF on my stereo 'scope,
same size as the base of the glass pot (an ex candle holder). Works surprisingly well.

Thanks for showing this trick !

mrsonchus wrote: ↑

Sun Dec 08, 2019 7:59 pm

An intriguing image, very nice.

Same thought!

Piter_ wrote: ↑

Sat Dec 07, 2019 9:33 pm

Thickness off the bottom varies between different plates, and you can find ones with 150 um thick bottom, suitable for high numerical aperture objectives.

Thanks in advance for specific information: the ones with 150um thick bottom, with which high numerical aperture objective can they be used ?

... Thanks for posting this, Michael. I find the fluorescence analysis of tiny particles interesting. Edit : The classification by the original researchers of particles as <5mm, <333 um in the Introduction section of their article seems indeed to me non-decisive. However, their aim is clearly defin...

I have operated both 12V/50W halogen lamps of the Nikon Optiphot with non-Nikon, relatively new general-purpose bench DC variable power supplies; the current needed was about 2.5-3A at most (I think; can double check if of interest). For this to work, on the lower (trans-illumination) lamp, I bypass...

Both images are very nice !
On the one hand, the DIC appears to be 3D, on the other hand, I remember that it is not necessarily true.
The COL on the other hand is quite similar to phase contrast. I guess that it is nearer the "true" image (where true=SEM, but only my guess).

P.S. since Numount is a replacement for Canada Balsam, I would check about its RI.

Thank you very much for your comments I am a newbie in the permanent slide montage. Excuse me, I confused the Shellac with nail polish. I will investigate about this gum. We agree with the crazy idea: I had planned to mount the sugar in high-grade fructose syrup. The problem is that it is no longer...

Undiluted sugar has a refractive index of 1.56. Today we have tried different transparent non-toxic and solvent-free glues, but the sugar has diluted after a few minutes. On the other hand, with superglue type acrylics sugar does not resist either. ¿Euparal damage sugar? The option of mounting suga...

Hi and welcome. Phase contrast images are often less impressive than DIC images, and they indeed display halos around objects. Yet, they are much better than brightfield for transparent water organisms. I highly recommend PC. And I think that a trinocular head for the microscope is a must. Remember,...

Thanks a lot, Oliver !

You’ll need a vertical facing filament to fill the aperture at high numerical apertures. That's good to know. Thank you. My current Optiphot has a horizontal filament so it will be interesting to compare the two bulb varieties under various conditions. The bulb stated in the Optiphot manual is a Ph...

Hi together, as far as I know these Zeiss n.a. 1,6 objectives were used with a different immersion medium and had very fragile front lens mounting. The n.a. 2,4 mountant must have been arsenic yellow medium. These objectives were probably available until the TEM and SEM became available. Today it w...

Welcome, leor333 from Israel as well ! While I never tried such camera connection - my Sony Handyman is an earlier model than yours - I can suggest some tips. Since the camera lens is not exchangeable, your option of connecting it to the microscope is afocal. That is, above the eyepiece of the micro...

Thanks 75RR for the post of the 4900x enlargement. It is impressive, and as previously discussed, was achieved by means of illumination, condenser, media and slide optimization; likely a 1.6NA objective alone would not provide such nice details.

Two different thoughts, of slight relevance: 1. I recently tried to stain marine foraminifera with common stains - methylene blue, Congo red and others; I will post the results if of interest, the bottom line is that the dye is washed away by water and only a tiny fraction of it is permanent. 2. Eas...

And if I dry the moisture fast will cause some damage equally? Sorry, I do not quite understand the question. The microscope should not be under condensation, never ever. There is no way to wipe the condensed water fast enough to prevent damage, if condensation happens again and again. If permissib...

I think that a 12V car lamp from an Automobile parts store or even Home Depot might work. Just a guess.

Hello everyone, my problem is today I have seen that on top of the slide turret and where the prisms are where the eyepieces go but on the outside I have seen that there was some condensed moisture, which was removed with toilet paper and a stick , and I have covered it again with the plastic, but ...

Here is another good reference on asbestos and dispersion, from the late R. Pavlis http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artmay08/rp-dispersion.html Regardless of asbestos analysis, it seems that dispersion staining can be fun, must find time to play w...

The 4X/0.1 objective almost certainly is DIN standard so its parfocality distance is 45mm. And its working distance large, ca 2cm as far as I remember. It will be usable (not great) on many 160mm tube length microscopes, new or old (except for some very old microscopes that accepted 33-37mm parfocal...

Are you sure the lamp houses need any grease ??

Hi everyone, my name is John. I've recently acquired an Olympus BX40 in great condition and hoping to get stuck into photomicrography. I've been a passionate photographer for many years now and getting my hands on a microscope to take photos through is just another aspect of that passion. But there...