Search found 3112 matches

by Hobbyst46
Fri Jan 22, 2021 11:12 am
Forum: Microscopes and optics
Topic: How to check out Zeiss darkfield 1.2-1.4 NA "ultracondenser" without a Zeiss microscope?
Replies: 24
Views: 409

Re: How to check out Zeiss darkfield 1.2-1.4 NA "ultracondenser" without a Zeiss microscope?

All my equipment is old Zeiss (west), originally all parts are designed to work together. Since you have the matching microscope, another related question, if you don't mind: Is there a lens (called the field lens or collimator by some manufacturers, not sure about Zeiss) between the condenser and ...
by Hobbyst46
Thu Jan 21, 2021 5:19 pm
Forum: Microscopes and optics
Topic: What even is this: Plan Apo 40x/1.0 W infinity f=180 unbranded m25 75mm long
Replies: 9
Views: 146

Re: What even is this: Plan Apo 40x/1.0 W infinity f=180 unbranded m25 75mm long

Hi Doron, I thought of a thermocouple as they are small. The more common PT or NTC sensors would be too big to fit next to the front lens. I don't know the electrical resistance of thermocouples, but a resistance measurement might help to narrow down the selection of opportunities. These could also...
by Hobbyst46
Thu Jan 21, 2021 3:22 pm
Forum: Illumination Techniques
Topic: scan lines in my camera image
Replies: 3
Views: 53

Re: scan lines in my camera image

It often happens with LED light. You might try to lower the exposure speed - expose for longer peridos. 1/15 s instead of 1/30 s, etc.
by Hobbyst46
Thu Jan 21, 2021 11:21 am
Forum: Microscopes and optics
Topic: What even is this: Plan Apo 40x/1.0 W infinity f=180 unbranded m25 75mm long
Replies: 9
Views: 146

Re: What even is this: Plan Apo 40x/1.0 W infinity f=180 unbranded m25 75mm long

Interesting catch! With all the heating it would make sense to measure the temperature. Is it possible that the tiny elements next to the front lens are a thermocouple? They put out a tiny voltage, perhaps you can measure it. Bob Bob, indeed, that wiring in the plane of the front lens very likely l...
by Hobbyst46
Thu Jan 21, 2021 10:53 am
Forum: Microscopes and optics
Topic: How to check out Zeiss darkfield 1.2-1.4 NA "ultracondenser" without a Zeiss microscope?
Replies: 24
Views: 409

Re: How to check out Zeiss darkfield 1.2-1.4 NA "ultracondenser" without a Zeiss microscope?

...correct immersion gap... This is basically why I was wondering. Practical setup issues aside, once one of these reflective darkfield condensers is set at the correct working distance in the microscope it was originally designed for, is the field diaphragm expected to be imaged in the object plan...
by Hobbyst46
Wed Jan 20, 2021 8:46 pm
Forum: Microscopes and optics
Topic: What even is this: Plan Apo 40x/1.0 W infinity f=180 unbranded m25 75mm long
Replies: 9
Views: 146

Re: What even is this: Plan Apo 40x/1.0 W infinity f=180 unbranded m25 75mm long

Heated by two gadgets. The top one - the brown tape - is a heating tape for use. Electrical. The collar with inlet and outlet tubing is likely a water circulation collar, to heat the nose of the objective. Was possibly used for the study of live cells at 37C; the cells were sitting in a petri dish o...
by Hobbyst46
Wed Jan 20, 2021 8:39 pm
Forum: Camera systems and imaging
Topic: Chinese Unbranded 48mp C-mount Industrial HDMI Camera
Replies: 11
Views: 150

Re: Chineses Unbranded 48mp C-mount Industrial HDMI Camera

hi mrsonchus,
Is the camera not supported by Toupview ? or MICAM perhaps ?
by Hobbyst46
Wed Jan 20, 2021 9:15 am
Forum: Microscopes and optics
Topic: Silicone immersion objectives - any experience?
Replies: 29
Views: 465

Re: Silicone immersion objectives - any experience?

A 100X1.25 SI objective like the one shown in the bottom photo must be in one of my drawers. If I can find it, attach to the DIN micoscope via a spacer ring to create some parfocality, and get some silicon oil, then it will be interesting to test. Viktor - which specimen do you plan to image with th...
by Hobbyst46
Mon Jan 18, 2021 8:49 am
Forum: Miscellaneous
Topic: Mental struggles and microscopy
Replies: 16
Views: 348

Re: Mental struggles and microscopy

Turning/milling is IMO a great skill, in which one can become an expert and produce highly precise items, depending on motivation and will to excel. Furthermore, turning and milling are creativity, and I believe they can become a hobby as well. Although 3D printing is here, making quality and precis...
by Hobbyst46
Mon Jan 18, 2021 7:16 am
Forum: Beginner's corner
Topic: Need help figuring out which compound microscope I should buy.
Replies: 5
Views: 120

Re: Need help figuring out which compound microscope I should buy.

Hello Everybody, I haven't gotten a microscope since I was about 10 years old....I'm now 72. That first microscope had no light, had little clips to hold the slides in place, (which constantly fell out), and rediculous lenses that produced very blurry views at higher power. So, here I am, consideri...
by Hobbyst46
Sun Jan 17, 2021 9:04 pm
Forum: Introduce yourself
Topic: Hello from Essex, UK
Replies: 2
Views: 49

Re: Hello from Essex, UK

Sweet kid !!!
Could be a sales promotion poster for the BH2 (not BL2) microscope. Enjoy !
by Hobbyst46
Sun Jan 17, 2021 9:49 am
Forum: Microscopes and optics
Topic: Silicone immersion objectives - any experience?
Replies: 29
Views: 465

Re: Silicone immersion objectives - any experience?

Thanks Michael and Viktor,
Incidentally, I am sure I have once seen an old 100X objective - almost certainly Olympus - that bore the mark "SI". In retrospect, might have been short for Silicone Immersion ?
by Hobbyst46
Sat Jan 16, 2021 11:27 pm
Forum: Microscopes and optics
Topic: Silicone immersion objectives - any experience?
Replies: 29
Views: 465

Re: Silicone immersion objectives - any experience?

Maybe not silicone immersion? https://en.wikipedia.org/wiki/Solid_immersion_lens https://www.twi-global.com/pdfs/equipment-factsheets/Automated-light-microscope-and-image-analyser.pdf Very interesting. Indeed SIL (solid imm.) yields super resolution; there is a lens that is made of high refractive ...
by Hobbyst46
Sat Jan 16, 2021 9:52 pm
Forum: Microscopes and optics
Topic: How to check out Zeiss darkfield 1.2-1.4 NA "ultracondenser" without a Zeiss microscope?
Replies: 24
Views: 409

Re: How to check out Zeiss darkfield 1.2-1.4 NA "ultracondenser" without a Zeiss microscope?

Tried to imitate your flashlight experiment on my Ultracondenser and did not find any similar effects to those on yours. Yes, I discovered delamination in both objectives and eyepieces from Zeiss. Not on condensers (two simple condensers and three phase contrast condensers, although I did not check ...
by Hobbyst46
Sat Jan 16, 2021 10:44 am
Forum: Microscopes and optics
Topic: How to check out Zeiss darkfield 1.2-1.4 NA "ultracondenser" without a Zeiss microscope?
Replies: 24
Views: 409

Re: How to check out Zeiss darkfield 1.2-1.4 NA "ultracondenser" without a Zeiss microscope?

Hans, Here are posts about my experience with the Ultracondenser. On mine, as received, paint was flaking off but I managed to fix it. It works very well and produces excellent DF with 40X0.75, 40X1.0 (oil) and even a fairly nice DF (or at least oblique) with an old achromat 100X1.25 (oil) - see lin...
by Hobbyst46
Fri Jan 15, 2021 6:57 pm
Forum: Introduce yourself
Topic: Happy to join you folks on this very informative forum!
Replies: 2
Views: 105

Re: Happy to join you folks on this very informative forum!

Simple inexpensive generic 5MP eyepiece cameras are usable on various microscopes. The only requirement is the inner diameter of the photo tube, which in most cases is 23.2 mm.
I have a 5MP USB eyepiece camera with which I recorded decent images from Zeiss, Olympus and Nikon microscopes.
by Hobbyst46
Fri Jan 15, 2021 3:31 pm
Forum: Microscopes and optics
Topic: Need help deciding between Motic BA310E & Panthera CC
Replies: 9
Views: 227

Re: Need help deciding between Motic BA310E & Panthera CC

A very slight (IMHO) advantage of the 40X UC objective. For darkfield, a lot of light is needed. A 30W halogen or 3W LED on the 310 model does not sound extremely bright. On the other hand, the Panthera specs boast full Kohler illumination. That would be an advantage. My own preference would be agai...
by Hobbyst46
Fri Jan 15, 2021 10:59 am
Forum: Microscopes and optics
Topic: Silicone immersion objectives - any experience?
Replies: 29
Views: 465

Re: Silicone immersion objectives - any experience?

My occasional experience with low-density silicone oil (not for microscopy) is that it IS messy. On the other hand it is very stable, much more so than any natural oil. Will not deteriorate with time. And fairly expensive - since the smallest package is way larger to what we need for microscopy.
by Hobbyst46
Thu Jan 14, 2021 5:41 pm
Forum: Camera systems and imaging
Topic: Simple dslr adapter hack for budget scopes.
Replies: 9
Views: 428

Re: Simple dslr adapter hack for budget scopes.

If anyone is interested, Oliver reviewed the Chinese dslr adapter a year ago: https://www.youtube.com/watch?v=Ju8rgeJr3bI Louise For an old finite optics microscopes in which spatial and color corrections are done in both the objective and eyepiece, I doubt that those DSLR adapters (when they inclu...
by Hobbyst46
Thu Jan 14, 2021 2:28 pm
Forum: Beginner's corner
Topic: Reichert received and a question
Replies: 31
Views: 485

Re: Reichert received and a question

Out of curiosity, I spotted this ad "10pc R155zz (5/32" x 5/16" x 1/8") Metric Shielded Ball Bearing Bearings R155" https://www.ebay.com/itm/10pc-R155zz-5-32-x-5-16-x-1-8-Metric-Shielded-Ball-Bearing-Bearings-R155/153339567359?hash=item23b3c01cff:g:QcsAAOSw9N1Vt6iX the data sheet indicates Imperial ...
by Hobbyst46
Wed Jan 13, 2021 10:45 pm
Forum: Beginner's corner
Topic: Looking for an older scope... I think
Replies: 7
Views: 174

Re: Looking for an older scope... I think

If the chief aim of the microscope is fluorescence, epi-fluorescence that is, the condenser is less important. High NA objectives on the other hand are very important for fluorescence. Low light calls for high NA. IMHO, given that the fluorescence is a very limited assortment of wavelengths, probabl...
by Hobbyst46
Wed Jan 13, 2021 9:41 pm
Forum: Beginner's corner
Topic: Looking for an older scope... I think
Replies: 7
Views: 174

Re: Looking for an older scope... I think

There are high intensity commercial LED lamps for microscopy, including power supply and dimmer, by the name CoolLED. Pretty expensive. Can be attached to an Olympus BX microscope (and other microscopes), so I believe can be interfaced to a BH2 stand. One still needs the epi-illumination field aper...
by Hobbyst46
Wed Jan 13, 2021 9:22 pm
Forum: Beginner's corner
Topic: Looking for an older scope... I think
Replies: 7
Views: 174

Re: Looking for an older scope... I think

There are high intensity commercial LED lamps for microscopy, including power supply and dimmer, by the name CoolLED. Pretty expensive. Can be attached to an Olympus BX microscope (and other microscopes), so I believe can be interfaced to a BH2 stand. One still needs the epi-illumination field apert...
by Hobbyst46
Tue Jan 12, 2021 8:20 pm
Forum: Pictures and Videos
Topic: A marine diatom with oblique lighting
Replies: 15
Views: 305

Re: A marine diatom with oblique lighting

I like the oblique illumination photos ! well done.
by Hobbyst46
Mon Jan 11, 2021 4:00 pm
Forum: Microscopes and optics
Topic: Olympus BH2 BHM?
Replies: 7
Views: 449

Re: Olympus BH2 BHM?

... 1. is it possible with this infinity optic to use say Olympus SPlan 160 mm tube objective with adapter? Will it be optically OK? I am afraid my comment about the RMS was irrelevant. Sorry. The BHM is an infinity corrected system, SPlans and Dplans are 160mm objectives, so incompatible. The comp...
by Hobbyst46
Mon Jan 11, 2021 1:56 pm
Forum: Microscopes and optics
Topic: Olympus BH2 BHM?
Replies: 7
Views: 449

Re: Olympus BH2 BHM?

1. NeoSplan objectives have 26mm threads, not 25mm. There must be sleeve adapters from 25 or 26mm to RMS. Non metallurgical SPlans and DPlans are RMS. 2. If the microscope has been used for metallurgy, where is the epi-illuminator. 3. A BH2 in good mechanical condition is a pleasure to have. 4. Obje...
by Hobbyst46
Mon Jan 11, 2021 1:38 pm
Forum: Pictures and Videos
Topic: Naphrax vs Pleurax vs Cameras vs Me!
Replies: 13
Views: 587

Re: Naphrax vs Pleurax vs Cameras vs Me!

Certainly even better images ! improved sharpness, higher resolution and less CA, supporting the hypothesis that the Naphrax slide is OK, and the images can be improved via the photographic gear. I think that the Canon 1100D altogether yields slightly better pictures than the Toupcam. Are you using ...
by Hobbyst46
Sun Jan 10, 2021 10:26 pm
Forum: Pictures and Videos
Topic: Naphrax vs Pleurax vs Cameras vs Me!
Replies: 13
Views: 587

Re: Naphrax vs Pleurax vs Cameras vs Me!

Nice choice, limonene as substitute for toluene. Likely, it will dissolve Naphrax well (my guess). Its disadvantage for Naphrax mounting is its high boiling point of 176C. Hence the slide must be heated long enough to remove the limonene, otherwise the RI will not reach 1.7. Of course heating the sl...
by Hobbyst46
Sun Jan 10, 2021 9:09 pm
Forum: Pictures and Videos
Topic: Naphrax vs Pleurax vs Cameras vs Me!
Replies: 13
Views: 587

Re: Naphrax vs Pleurax vs Cameras vs Me!

The Pleurax slide seems much better to me. My dslr pics always seem to come out not very clear - no sharp focus. I've been using a Chinese 'dslr adapter' for the trinocular attachment (which has an inbuilt lens) - not sure if the images through that are as good as they might be. Any thoughts, sugge...
by Hobbyst46
Sun Jan 10, 2021 1:11 pm
Forum: Pictures and Videos
Topic: Some homegrown diatoms
Replies: 7
Views: 485

Re: Some homegrown diatoms

...Nothing fancy, I just took some samples in small jars back in November. They sit on a window sill in my living room and in the (winter) light. But they are considerably warmer than outdoor ambient temperatures which have been sub zero here recently (brrr!). I've kept them topped up with boiled w...