This old thread shows (see for example response #20) a relatively inexpensive first surface metal mirror. Was done and works great.
"Re: Seeking advice on glueing the 45 deg mirror under the field aperture back in place (Zeiss) - Solved."
Search found 4291 matches
- Sat May 11, 2024 2:21 pm
- Forum: Microscopy accessories
- Topic: B&L B Stand
- Replies: 6
- Views: 774
- Sun May 05, 2024 11:25 am
- Forum: Identification help
- Topic: Can someone help identify Cyclotella meneghiniana ?
- Replies: 6
- Views: 188
Re: Can someone help identify Cyclotella meneghiniana ?
Could be that the left image is a top view in post #1 (the valves lie horizontally) and the right image is a girdle view (the valves lie vertically) of the same diatom ?
- Fri May 03, 2024 7:44 am
- Forum: Identification help
- Topic: Diatom. ?
- Replies: 5
- Views: 145
Re: Diatom. ?
Yes IMHO. Looks like Licmophora.
Maybe not fractured, just caught and photographed from different viewing angles.
Maybe not fractured, just caught and photographed from different viewing angles.
- Thu Apr 25, 2024 8:39 pm
- Forum: Pictures and Videos
- Topic: The Onion Skin Devil
- Replies: 4
- Views: 285
Re: The Onion Skin Devil
Taking microscope images of onion skin is supposed to be an elementary exercise usually not worth the efforts of serious hobbyists. I found it pretty hard to do. For one thing onions make gaseous fumes which tend to fog lenses. I also found the skins difficult to gracefully cut. Anyway I got some p...
- Tue Apr 23, 2024 8:39 pm
- Forum: Microscopes and optics
- Topic: Partially blocked Leica objective
- Replies: 29
- Views: 1175
- Mon Apr 22, 2024 10:51 am
- Forum: My microscope
- Topic: My Travel Microscopes
- Replies: 20
- Views: 6321
Re: My Travel Microscopes
...I have used the hybrid oculars with Zeiss achromats and plan objectives (have not tried them with my Neofluars yet, although Rolf tested that out, too I believe). To my eyes the resulting image looks good, at least as good as an afocal configuration... IMO, based on tests with an APS-C camera an...
- Sat Apr 20, 2024 7:29 pm
- Forum: Home-made microscope adaptations
- Topic: half mirror
- Replies: 2
- Views: 266
Re: half mirror
Assuming you refer to the flat mirror installed at an angle of 45 degrees above the nosepiece: it is a 50/50 beam splitter.
- Sat Apr 20, 2024 10:29 am
- Forum: Microscopes and optics
- Topic: Partially blocked Leica objective
- Replies: 29
- Views: 1175
Re: Partially blocked Leica objective
...I was looking into these types of dishes before: https://www.thermofisher.com/order/catalog/product/150680 I can easily go through 20-30 plates in a month though, and paying $200 a month for dishes is not an option... If cost is the only reason for not using the glass-bottom petri dishes: they a...
- Tue Apr 16, 2024 10:21 am
- Forum: Microscopes and optics
- Topic: how scared should I be of mercury lamps
- Replies: 14
- Views: 781
Re: how scared should I be of mercury lamps
In proper use by a trained microscopist exactly according to the manufacturer's usage and safety instructions , and provided that the history of the said specific mercury illuminator is known, no need to fear IMHO. For transportation, I would leave the lamp in the housing. Rather, inspect it visuall...
- Fri Apr 12, 2024 8:37 pm
- Forum: Microscopes and optics
- Topic: Centering a Zeiss circular stage
- Replies: 6
- Views: 732
Re: Centering a Zeiss circular stage
In a thread on this forum (https://www.microbehunter.com/microscopy-forum/viewtopic.php?f=5&t=6115&p=89593#p89593), 75RR provided a method for centering a circular stage on a Zeiss microscope (see below) 75RR's Instructions and Figure: In order to centre the stage we need an eyepiece with a reticul...
- Fri Apr 12, 2024 8:16 pm
- Forum: Illumination Techniques
- Topic: Prepared slide set for demonstrating contrast techniques?
- Replies: 8
- Views: 666
Re: Prepared slide set for demonstrating contrast techniques?
IMHO, a commercial permanent strew slide (for relatively low cost) or arranged slide (where budget is not limiting) of diatoms sounds to me very practical, in spite of the "vintage" atmosphere that it may broadcast. It will clearly demonstrate all mentioned modalities except for polarization and flu...
- Wed Apr 10, 2024 8:18 am
- Forum: Microscopes and optics
- Topic: Centering a Zeiss circular stage
- Replies: 6
- Views: 732
Re: Centering a Zeiss circular stage
...I love the Zeiss rotating biological stage so much I've adapted a couple of them to my Oly BH-2s... That is interesting, KurtM ! the Zeiss stage is attached to the Zeiss Standard stand with four screws arranged in an arc. Did you drill through the BH-2 stage carrier ? is the thickness of the BH-...
- Sat Mar 30, 2024 8:20 pm
- Forum: Digital processing
- Topic: Chromatic aberrations filtering
- Replies: 52
- Views: 5001
Re: Chromatic aberrations filtering
An amber KR12 filter works for me. It removes much of the 450nm blue tint of a 6500K LED. The filter was not costly then (in 2018/2019). The 85A did part of the job, not enough IMO. Searching "KR12" on the forum will lead to some 4-5 years old posts about LED light filtration. Amber filters were use...
- Fri Mar 29, 2024 9:34 pm
- Forum: Microscopes and optics
- Topic: Olympus IMT-2 mystery
- Replies: 17
- Views: 2935
Re: Olympus IMT-2 mystery
... I will take some detail photos tomorrow … but meanwhile; the 50W lamp-house is detailed on FIG 7 of this: https://www.alanwood.net/downloads/olympus-imt-2-parts-lists-diagrams.pdf Michael, Fig 20 on that list hints (maybe) that there was more than one illumination housing. So, while a 50W lamp ...
- Sun Mar 24, 2024 9:46 am
- Forum: Beginner's corner
- Topic: Centering & adjusting the Leitz 100Z lamp?
- Replies: 12
- Views: 2288
Re: Centering & adjusting the Leitz 100Z lamp?
On a proper setup, the condenser top lens should be able to nearly touch the slide bottom - within less than a millimeter. At least, to allow oiling the condenser to the slide for oil immersion imaging. However, the geometry of the stage and condenser carrier could allow the top lens to emerge too m...
- Sat Mar 23, 2024 9:12 pm
- Forum: Pictures and Videos
- Topic: 4 different 40X Reichert phase infinity objectives used in an AO 10 stand.
- Replies: 3
- Views: 936
Re: 4 different 40X Reichert phase infinity objectives used in an AO 10 stand.
Very nice images. IMHO, No. 3 is better than the others, resolution-wise. The others suffer some from the halos.sharpness.
Would be interesting to see those (at least one of them) photographed with a fixed camera on the microscope rather then a phone.
Would be interesting to see those (at least one of them) photographed with a fixed camera on the microscope rather then a phone.
- Sun Mar 10, 2024 6:46 am
- Forum: Illumination Techniques
- Topic: Epi Kölher illumination and infinity corrected systems
- Replies: 7
- Views: 1701
Re: Epi Kölher illumination and infinity corrected systems
Also, I took a look at how my Olympus BH2-MA was made and it seems they added some diffuser inside of the illumination tube. Is this common practice? But why it is needed if they use some kind of Köhler illumination? In the old Zeiss Standard illumination collector, next to the light bulb, a mild d...
- Sat Mar 09, 2024 9:16 pm
- Forum: Illumination Techniques
- Topic: Epi Kölher illumination and infinity corrected systems
- Replies: 7
- Views: 1701
Re: Epi Kölher illumination and infinity corrected systems
Thank you. But in the case of epi illumination, the objective is the condenser right? It is the same than an infinity condenser? That's why I was wondering if parallel beams were needed in this specific case. Wild guess: Kohler is needed in the epi- and infinity optics as well, since the illuminati...
- Fri Mar 08, 2024 3:56 pm
- Forum: Illumination Techniques
- Topic: Condenser Alignment.
- Replies: 17
- Views: 2516
Re: Condenser Alignment.
Yes, as shown in the photo.
When the condenser and auxiliary lens are aligned, any blue fringe (if at all) will be symmetric, around the open iris.
When the condenser and auxiliary lens are aligned, any blue fringe (if at all) will be symmetric, around the open iris.
- Thu Mar 07, 2024 8:04 am
- Forum: Microscopes and optics
- Topic: Olympus CX43 any thoughts ,made in China apparently
- Replies: 19
- Views: 1660
Re: Olympus CX43 any thoughts ,made in China apparently
I found one called a BM2000 ( looks a bit of a Nikon copy) on an Australian site they sell Nikon also. https://microscopes.com.au/collections/phase-contrast/products/bm2000-phase-contrast-microscope Looks ok but focus only on left side, Strange that a microscope costing that much does not include t...
- Wed Mar 06, 2024 11:11 pm
- Forum: Illumination Techniques
- Topic: Condenser Alignment.
- Replies: 17
- Views: 2516
Re: Condenser Alignment.
Apo, a splendid contribution as well as thought provoking. Like Hobbyst46 I have Zeiss scopes fitted with the same AchApl 1.4 phase condensers. Never thought of centering lens of condenser proper. However below the condenser lies an auxiliary lens that does have centration control which I have neve...
- Wed Mar 06, 2024 10:33 pm
- Forum: Illumination Techniques
- Topic: Condenser Alignment.
- Replies: 17
- Views: 2516
Re: Condenser Alignment.
centration - from the Merriam Webster Dictionary it appears to be a synonym of centering.
declipse - sounds to me a great and meaningful word, that is apparently missing in the same dictionary...
declipse - sounds to me a great and meaningful word, that is apparently missing in the same dictionary...
- Wed Mar 06, 2024 4:39 pm
- Forum: Illumination Techniques
- Topic: Condenser Alignment.
- Replies: 17
- Views: 2516
Re: Condenser Alignment.
An excellent guide, Apo, thanks ! How would you suggest to test and align a turret achromat-aplanat 1.4 phase contrast condenser ? the basic lens gives an NA=0.32. It is fixed inside the body and I did not see any set screws around (though never seriously checked). The top lens, which makes it NA=1....
- Tue Mar 05, 2024 7:39 am
- Forum: Digital processing
- Topic: Chromatic aberrations filtering
- Replies: 52
- Views: 5001
Re: Chromatic aberrations filtering
In my experience, such unsymmetrical CA might originate from a misaligned condenser.
- Wed Feb 21, 2024 9:47 pm
- Forum: Microscopy accessories
- Topic: What tip size for micropipette
- Replies: 7
- Views: 1825
Re: What tip size for micropipette
This old post (Oct 4, 2018) by "Question for Pipette users" by forum member 75RR, and response #23, might help.
- Sun Feb 11, 2024 4:39 pm
- Forum: Camera systems and imaging
- Topic: Recommendation Camera for Zeiss Axiovert 25CFL with 1x Adapter
- Replies: 11
- Views: 2017
Re: Recommendation Camera for Zeiss Axiovert 25CFL with 1x Adapter
Some Olympus fluorescence-sensitive microscope cameras have a small sensor, 1/2.5" I think, and are meant to be used with a ~0.5X reducing lens for decent field coverage. The following might be somewhat relevant: https://www.microbehunter.com/microscopy-forum/viewtopic.php?f=9&t=18847&p=138958&hilit...
- Fri Feb 09, 2024 8:16 am
- Forum: Microscopes and optics
- Topic: Olympus SZ-FLR-200
- Replies: 27
- Views: 4531
Re: Olympus SZ-FLR-200
Do you really need 365nm wavelength ? if not, if 395nm or 420nm would be OK for the type of fluorescence you wish to see, I would opt for either the 395 or the 420 rather than the 365, for personal safety (eyesight !) reasons at least. And pay even less - ~130 USD as listed. I am not sure, but I ve...
- Thu Feb 08, 2024 7:18 pm
- Forum: Microscopes and optics
- Topic: Olympus SZ-FLR-200
- Replies: 27
- Views: 4531
Re: Olympus SZ-FLR-200
Reviving this thread again—I've been thinking about how I might observe fluorescence with my stereomicroscope, and I just learned that Amscope offers several versions of UV LED gooseneck lights, e.g.: https://amscope.com/products/led-6w-uv365?variant=40347665662127 , https://amscope.com/products/fb...
- Sun Feb 04, 2024 10:06 pm
- Forum: Microscopes and optics
- Topic: Stereoscope vs. Compound Microscope at Given Total Magnification
- Replies: 7
- Views: 1048
Re: Stereoscope vs. Compound Microscope at Given Total Magnification
The field of view is the: field number/power of objective . Same calculation for stereo and compound. Also same total magnification calculation. The table above shows that the Spencer 9X eyepiece has a FN of 19.7 (probably 20?). For example, on my old Olympus stereo, the eyepiece FN is 22, so for a ...
- Sun Feb 04, 2024 8:32 pm
- Forum: Microscopes and optics
- Topic: Stereoscope vs. Compound Microscope at Given Total Magnification
- Replies: 7
- Views: 1048
Re: Stereoscope vs. Compound Microscope at Given Total Magnification
Hello. This is a curiosity question, not trying to solve a specific problem. Could someone please describe the expected pros and cons — or direct folks to a website — of using a stereoscope (dissecting microscope) vs. a compound microscope at a given total magnification. For example, both equipped ...