MicrobeHunter.com Microscopy Forum

In a thread on this forum (https://www.microbehunter.com/microscopy-forum/viewtopic.php?f=5&t=6115&p=89593#p89593), 75RR provided a method for centering a circular stage on a Zeiss microscope (see below) 75RR's Instructions and Figure: In order to centre the stage we need an eyepiece with a reticul...

IMHO, a commercial permanent strew slide (for relatively low cost) or arranged slide (where budget is not limiting) of diatoms sounds to me very practical, in spite of the "vintage" atmosphere that it may broadcast. It will clearly demonstrate all mentioned modalities except for polarization and flu...

...I love the Zeiss rotating biological stage so much I've adapted a couple of them to my Oly BH-2s... That is interesting, KurtM ! the Zeiss stage is attached to the Zeiss Standard stand with four screws arranged in an arc. Did you drill through the BH-2 stage carrier ? is the thickness of the BH-...

An amber KR12 filter works for me. It removes much of the 450nm blue tint of a 6500K LED. The filter was not costly then (in 2018/2019). The 85A did part of the job, not enough IMO. Searching "KR12" on the forum will lead to some 4-5 years old posts about LED light filtration. Amber filters were use...

... I will take some detail photos tomorrow … but meanwhile; the 50W lamp-house is detailed on FIG 7 of this: https://www.alanwood.net/downloads/olympus-imt-2-parts-lists-diagrams.pdf Michael, Fig 20 on that list hints (maybe) that there was more than one illumination housing. So, while a 50W lamp ...

On a proper setup, the condenser top lens should be able to nearly touch the slide bottom - within less than a millimeter. At least, to allow oiling the condenser to the slide for oil immersion imaging. However, the geometry of the stage and condenser carrier could allow the top lens to emerge too m...

Very nice images. IMHO, No. 3 is better than the others, resolution-wise. The others suffer some from the halos.sharpness.
Would be interesting to see those (at least one of them) photographed with a fixed camera on the microscope rather then a phone.

Also, I took a look at how my Olympus BH2-MA was made and it seems they added some diffuser inside of the illumination tube. Is this common practice? But why it is needed if they use some kind of Köhler illumination? In the old Zeiss Standard illumination collector, next to the light bulb, a mild d...

Thank you. But in the case of epi illumination, the objective is the condenser right? It is the same than an infinity condenser? That's why I was wondering if parallel beams were needed in this specific case. Wild guess: Kohler is needed in the epi- and infinity optics as well, since the illuminati...

Yes, as shown in the photo.
When the condenser and auxiliary lens are aligned, any blue fringe (if at all) will be symmetric, around the open iris.

I found one called a BM2000 ( looks a bit of a Nikon copy) on an Australian site they sell Nikon also. https://microscopes.com.au/collections/phase-contrast/products/bm2000-phase-contrast-microscope Looks ok but focus only on left side, Strange that a microscope costing that much does not include t...

Apo, a splendid contribution as well as thought provoking. Like Hobbyst46 I have Zeiss scopes fitted with the same AchApl 1.4 phase condensers. Never thought of centering lens of condenser proper. However below the condenser lies an auxiliary lens that does have centration control which I have neve...

centration - from the Merriam Webster Dictionary it appears to be a synonym of centering.
declipse - sounds to me a great and meaningful word, that is apparently missing in the same dictionary...

An excellent guide, Apo, thanks ! How would you suggest to test and align a turret achromat-aplanat 1.4 phase contrast condenser ? the basic lens gives an NA=0.32. It is fixed inside the body and I did not see any set screws around (though never seriously checked). The top lens, which makes it NA=1....

In my experience, such unsymmetrical CA might originate from a misaligned condenser.

This old post (Oct 4, 2018) by "Question for Pipette users" by forum member 75RR, and response #23, might help.

Some Olympus fluorescence-sensitive microscope cameras have a small sensor, 1/2.5" I think, and are meant to be used with a ~0.5X reducing lens for decent field coverage. The following might be somewhat relevant: https://www.microbehunter.com/microscopy-forum/viewtopic.php?f=9&t=18847&p=138958&hilit...

Do you really need 365nm wavelength ? if not, if 395nm or 420nm would be OK for the type of fluorescence you wish to see, I would opt for either the 395 or the 420 rather than the 365, for personal safety (eyesight !) reasons at least. And pay even less - ~130 USD as listed. I am not sure, but I ve...

Reviving this thread again—I've been thinking about how I might observe fluorescence with my stereomicroscope, and I just learned that Amscope offers several versions of UV LED gooseneck lights, e.g.: https://amscope.com/products/led-6w-uv365?variant=40347665662127 , https://amscope.com/products/fb...

The field of view is the: field number/power of objective . Same calculation for stereo and compound. Also same total magnification calculation. The table above shows that the Spencer 9X eyepiece has a FN of 19.7 (probably 20?). For example, on my old Olympus stereo, the eyepiece FN is 22, so for a ...

Hello. This is a curiosity question, not trying to solve a specific problem. Could someone please describe the expected pros and cons — or direct folks to a website — of using a stereoscope (dissecting microscope) vs. a compound microscope at a given total magnification. For example, both equipped ...

The only thing I can think of is to seal it with silicone along the edges between the metal and the glass, very carefully. Immersion oil penetrates many polymers, if left on them for many hours. Silicon sealing in general, is efficient against water, but less so against solvents and oils. Immersion...

An interesting thread. Good luck !
BTW, I would not use ammonia to clean brass metal parts.

I doubt that vacuum alone will eliminate trapped oil (if it is oil). Vacuum accelerates evaporation. Perhaps the abrupt decline of air pressure, when the vacuum pump was turned on, disintegrated a large oil drop to small droplets. Admittedly, small droplets will evaporate much faster than a large dr...

stjepo wrote: ↑

Fri Jan 19, 2024 2:28 pm

Any advice from you will help me.

Sorry and excuse my ignorance what is 'sa and ca'

Regards

Steve

Spherical aberrations and chromatic aberrations, respectively.

Just thought I would dig up this old gem of a thread to convey the story of my success in restoring the opaque black paint on my Zeiss Ultra condenser. The product I used was a rattle can of VHT wheel paint . I didn't buy it specifically for this purpose - I shamelessly salvaged it, full, from a ro...

Maybe there is no problem with the condenser - but the objective turret (nosepiece) is slightly off-position ?

In a complete Kohler setup, there is one more degree of freedom - the distance between the lamp and the collector optics. On my very old microscope for example, which dates from the 1950's, that distance can be adjusted by sliding the lamp housing slightly in or out, until the filament image is def...

In a complete Kohler setup, there is one more degree of freedom - the distance between the lamp and the collector optics. On my very old microscope for example, which dates from the 1950's, that distance can be adjusted by sliding the lamp housing slightly in or out, until the filament image is defo...

Oh, to clarify: this is the typical information posted by sellers about their microscope cameras, which is where I could use some guidance picking out the 3-4 important details to compare among several different camera offerings. HAYEAR USB3.0 Digital Microscope Camera 8 Mega Pixels USB Live Video ...