MicrobeHunter.com Microscopy Forum

zzffnn ... I am curious to know why using 1 to 2 stops up from the widest aperture "works best" for static subjects? I would have thought that having more overlap of relatively good focus would work but then again I do not really understand how the stacking algorithms work. Is what your saying the w...

With this image I used a single light-source and a ping pong ball. The light source is what is called a illuma fitting that is a tungsten halogen fitting using a dichroic reflector lamp designed to be dimmed. I did initially try with it up brighter but it seemed to wash out the detail. I could try i...

Still having trouble getting acceptable results through a microscope so thought I would have a go at something strictly macro with my Nikon D300S and a SP Tamron Di90 macro lens with around 280 mm extension at f22. Stacked 20 images. See attached image.

Thanks for all of the very useful information ... I will persevere with these and other objectives given what you guys have enlightened me with. I have a couple of Lomo APO's (160 mm tube length) on the way to me from Russia that I am looking forward to trying out. Many years ago while studying towa...

Apocronaut ... going by what you are saying what does this mean for the likes of one of the a apochromatic lenses that I am experimenting with i.e., a Carl Zeiss Jenna 40X / o.95. I know this is a extremely sharp objective but seems to have an extremely narrow depth of field ... it is a 190 mm tube-...

Thanks heaps for that information. The objectives do not show a NCG on them but the NA is written on them as follows. 11X NA = 0.35 22X NA = 0.65 40X NA = 0.95 94X NA = 1.40 Based on the responses does it mean I can add 30 mm to the tube length between the top of the tube and the euepiece? What abou...

BlackAntFootWeb.jpg Hi everybody. I am new to this forum so will tell all a little about myself. I am not completely new to microscopy as I have a Bachelor of science B.Sc., Biology/Human nutrition degree from Wollongong uni, NSW Australia ... a Post graduate diploma PGradDip., archaeology/paleoant...