Search found 119 matches

by Timemaster1212
Mon Aug 10, 2020 9:27 pm
Forum: Specimens, samples and slides
Topic: Euparal Protocols
Replies: 1
Views: 1088

Euparal Protocols

I’ve dipped my toes into the world on slide production with my first permanent slide: common housefly wing! While i do not currently have any images, i will post some after work, i noticed that the Euparal I purchased is still very liquid under the coverslip after ~40 hours. And i thought this was s...
by Timemaster1212
Mon Aug 10, 2020 7:07 pm
Forum: For forum members who want to buy and sell equipment
Topic: WTB Slide Storage Cabinet
Replies: 15
Views: 1263

Re: WTB Slide Storage Cabinet

BramHuntingNematodes wrote:
Mon Aug 10, 2020 6:11 pm
. We should start a slide trading club someday...
I second this! Also, i wonder how much trouble it would be to build one out of wood and metal. Hmm....
by Timemaster1212
Mon Aug 10, 2020 5:06 pm
Forum: For forum members who want to buy and sell equipment
Topic: WTB Slide Storage Cabinet
Replies: 15
Views: 1263

Re: WTB Slide Storage Cabinet

I do enjoy the 3d printed cabinet for a short term option! While i love the wooden cabinet it is way out of my budget
by Timemaster1212
Mon Aug 10, 2020 5:24 am
Forum: For forum members who want to buy and sell equipment
Topic: WTB Slide Storage Cabinet
Replies: 15
Views: 1263

WTB Slide Storage Cabinet

Hello all! I recently (like yesterday) began my journey in permanent slide making. However the issue of storsge came to mind, so I am interested in one! Does anyone have any they are willing to se, or any they recommend? Thanks!
by Timemaster1212
Sat Aug 08, 2020 6:29 am
Forum: Specimens, samples and slides
Topic: MIcroscopic Aquarium Slides
Replies: 7
Views: 1107

Re: MIcroscopic Aquarium Slides

How about this: https://www.carolina.com/protist-viewing-supplies/microaquarium/131016.pr An old science teacher gave me one in middle school when I started the hobby. They are quite fun for low magnification and non-critical work. I used it heavily for viewing seed shrimp and small micro-macro org...
by Timemaster1212
Fri Aug 07, 2020 7:40 pm
Forum: Illumination Techniques
Topic: Correct Allignment of a Series 4 Stand
Replies: 36
Views: 2640

Re: Correct Allignment of a Series 4 Stand

Image

Alrighty, i think i figured it out haha, camera issue LOL. I think this is the best image yet, let me know what yall think.
by Timemaster1212
Fri Aug 07, 2020 5:31 pm
Forum: Illumination Techniques
Topic: Correct Allignment of a Series 4 Stand
Replies: 36
Views: 2640

Re: Correct Allignment of a Series 4 Stand

So, the objectives on my stand are Dark M Phase contrast. Also, the view through the eyepiece is more grey than the image, which makes me wonder if the browning is a white balance issue, my canon is set to automatic. I am making another swab slide to show more properties of the image without being t...
by Timemaster1212
Thu Aug 06, 2020 5:47 pm
Forum: Illumination Techniques
Topic: Correct Allignment of a Series 4 Stand
Replies: 36
Views: 2640

Re: Correct Allignment of a Series 4 Stand

Sorry for the late replies! Yes i do have the intermediate lens on my stand, and you were right i was out of focus apo. I found a hydra while checking on one of my pond samples, and while not the best test (nearly all of the halos are debris out of focus), to my untrained eye it appears to have a mu...
by Timemaster1212
Tue Aug 04, 2020 7:19 am
Forum: Illumination Techniques
Topic: Correct Allignment of a Series 4 Stand
Replies: 36
Views: 2640

Re: Correct Allignment of a Series 4 Stand

Alright when i put my glasses on i realized how bad it really was haha, this should be a better after redoing the test.
Image
by Timemaster1212
Tue Aug 04, 2020 7:08 am
Forum: Illumination Techniques
Topic: Correct Allignment of a Series 4 Stand
Replies: 36
Views: 2640

Re: Correct Allignment of a Series 4 Stand

Alright! Progress (i think) made!

Same 20x magnification, no editing, just straight from the camera.
Image

Now the light path is dirty and the focus is not the best, however as a preliminary test of the new adjustments i think it looks much better.
by Timemaster1212
Mon Aug 03, 2020 6:04 pm
Forum: Illumination Techniques
Topic: Correct Allignment of a Series 4 Stand
Replies: 36
Views: 2640

Re: Correct Allignment of a Series 4 Stand

Oh and i forgot to add, if brightfield, the images look better through the eyepieces than through the camera, something i am sorting out still. However phase is a different monster, it all looks very off through the eye pieces and through the camera.
by Timemaster1212
Mon Aug 03, 2020 5:56 pm
Forum: Illumination Techniques
Topic: Correct Allignment of a Series 4 Stand
Replies: 36
Views: 2640

Re: Correct Allignment of a Series 4 Stand

Yessir, this is indeed a different stand. I retired that one and have been using it for parts. And perhaps my bulb is the source of all of this, i do not believe it is the proper one. And apo, i still do not see how one could focus the Bertrand lens with it built into the head. Is it prefocused then...
by Timemaster1212
Mon Aug 03, 2020 6:06 am
Forum: Illumination Techniques
Topic: Correct Allignment of a Series 4 Stand
Replies: 36
Views: 2640

Re: Correct Allignment of a Series 4 Stand

Wow, thank you everyone for the wealth of information, work caught me up today so sorry for the lack of communication. The microscope that is the topic of this thread I would call a modified Koehler type, having an easily adjusted filament bulb that can achieve an even , bright illumination field in...
by Timemaster1212
Sun Aug 02, 2020 5:51 am
Forum: Illumination Techniques
Topic: Correct Allignment of a Series 4 Stand
Replies: 36
Views: 2640

Re: Correct Allignment of a Series 4 Stand

LampCent.JPG
LampCent.JPG (34.31 KiB) Viewed 1702 times
As you can see, the lamp centering screws exist in the base. However the collecting lens directly in front of the bulb is frosted, making it (from what i read) impossible to center the filament.
by Timemaster1212
Sat Aug 01, 2020 6:03 pm
Forum: Illumination Techniques
Topic: Correct Allignment of a Series 4 Stand
Replies: 36
Views: 2640

Re: Correct Allignment of a Series 4 Stand

I do not, i have a built in Bertrand lens in my trinocular head that i use
by Timemaster1212
Sat Aug 01, 2020 6:05 am
Forum: Illumination Techniques
Topic: Correct Allignment of a Series 4 Stand
Replies: 36
Views: 2640

Re: Correct Allignment of a Series 4 Stand

Image
20x phase
If it helps this is the closest i can get to a test plate image haha. To me it seems very uneven and just off, if that makes any sense. However, everything was perfectly aligned, condenser speaking.
by Timemaster1212
Sat Aug 01, 2020 4:58 am
Forum: Illumination Techniques
Topic: Correct Allignment of a Series 4 Stand
Replies: 36
Views: 2640

Correct Allignment of a Series 4 Stand

Alright folks, I apologize for the noobish questions, however I am quite stuck in a very quickly sinking boat. As i continue to experiment with my microscope to get better images, something seems very off with my phase. So, i went back to square one: Kholer Illumination. While the simple explanation...
by Timemaster1212
Sun Jul 19, 2020 7:41 pm
Forum: Microscopy accessories
Topic: AO Series 4 Removable Nosepiece?
Replies: 2
Views: 681

Re: AO Series 4 Removable Nosepiece?

Oh those darn grub screws will be my bane. Thanks for the quick help!
by Timemaster1212
Sat Jul 18, 2020 5:51 am
Forum: Microscopy accessories
Topic: AO Series 4 Removable Nosepiece?
Replies: 2
Views: 681

AO Series 4 Removable Nosepiece?

Hello everybody. As i continue to improve (hopefully) my imaging, i am noticing a lot of blemishes from dust and other specks from a dirty light path, so i decided to clean my stand. However when it came time for me to remove the nose piece, it appears to be stuck. I know my stand has a removable no...
by Timemaster1212
Thu Jul 16, 2020 7:19 pm
Forum: My microscope
Topic: Zeiss Standard Junior - "Bundeswehr" - Bob's big travel microscope
Replies: 7
Views: 1469

Re: Zeiss Standard Junior - "Bundeswehr" - Bob's big travel microscope

Beautiful setup, I would love to hear where you take this stand!
by Timemaster1212
Sat Jun 27, 2020 7:15 pm
Forum: Beginner's corner
Topic: Where to find Diatom Test Slide
Replies: 1
Views: 679

Where to find Diatom Test Slide

Hello all, As i continue to experiment with my trinocular setup for imaging, it was brought to my attention that a test slide could help me fine tune my set up. However no mater where i looked, either i could not order one ( Klaus Kemp) or I had an extremely long wait time and risk of my slide being...
by Timemaster1212
Fri Jun 26, 2020 1:58 am
Forum: Pictures and Videos
Topic: My First Attempts at Digital Photomicrography
Replies: 44
Views: 3484

Re: My First Attempts at Digital Photomicrography

Not wishing to flood the active topics, this will be my last post (probably) on this just to show some more experimentation. I read that image stacking can decrease the noise in an image so i attempted this with combine ZP and got the following: https://i.imgur.com/Sb5DxrZ.jpg I am not sure if this ...
by Timemaster1212
Thu Jun 25, 2020 6:05 pm
Forum: Pictures and Videos
Topic: My First Attempts at Digital Photomicrography
Replies: 44
Views: 3484

Re: My First Attempts at Digital Photomicrography

So i changed my adapter set up, bringing back the tube and attaching the adapter at the bottom of the photo tube. This reduced the empty magnification and, with the blue light filter, made a more pleasant image with some touching up. Is it common for a filter to increase image exposure time dramatic...
by Timemaster1212
Thu Jun 25, 2020 6:02 am
Forum: Pictures and Videos
Topic: My First Attempts at Digital Photomicrography
Replies: 44
Views: 3484

Re: My First Attempts at Digital Photomicrography

Ah i did not consider empty magnification, i will look more into that. And I totally forgot to mention it, but it was an 43x objective with a 10x eyepiece acting as a projecting lens
by Timemaster1212
Thu Jun 25, 2020 3:02 am
Forum: Pictures and Videos
Topic: My First Attempts at Digital Photomicrography
Replies: 44
Views: 3484

Re: My First Attempts at Digital Photomicrography

So here is some more pictures with some differing settings The original picture was taken at 0"6 shutter speed and 100 ISO with Kholer illumination and in Bright field. https://i.imgur.com/Tt1QCPL.jpg Next i toyed around in Photo Pos Pro and got this as a result: https://i.imgur.com/aTrD4vz.jpg I ne...
by Timemaster1212
Wed Jun 24, 2020 7:32 pm
Forum: Pictures and Videos
Topic: My First Attempts at Digital Photomicrography
Replies: 44
Views: 3484

Re: My First Attempts at Digital Photomicrography

Well the issue is just mainly balancing shutter speed and iso, however i adjusted the exposer compensation on the camera just to experiment and it worked, however the pictures are still grainy. I’ll post some soon
by Timemaster1212
Wed Jun 24, 2020 5:39 pm
Forum: Pictures and Videos
Topic: My First Attempts at Digital Photomicrography
Replies: 44
Views: 3484

Re: My First Attempts at Digital Photomicrography

Some are in phase, however most are brightfield. As for the RAW file i would love to send one once i get home. I’ll use something more organized such as onion epidermis if this is better than trying to photograph rotifers.
by Timemaster1212
Wed Jun 24, 2020 11:29 am
Forum: Pictures and Videos
Topic: My First Attempts at Digital Photomicrography
Replies: 44
Views: 3484

Re: My First Attempts at Digital Photomicrography

Wow this is great! It seems i have a lot to do! I appreciate all the helpful tips and i love seeing people mess with the images haha. I believe a better light source and possibly figuring out the adapter set up with diatoms will be my next step. In the mean time I’ll try my hand at photo editing. I ...
by Timemaster1212
Wed Jun 24, 2020 3:51 am
Forum: Pictures and Videos
Topic: My First Attempts at Digital Photomicrography
Replies: 44
Views: 3484

Re: My First Attempts at Digital Photomicrography

Ah that’s interesting! I’ll keep practicing, and in the mean time look for a diatom test slide, i think this can help me fine tune a set up.
Edit: the only issue is sourcing one :lol:
by Timemaster1212
Wed Jun 24, 2020 2:38 am
Forum: Pictures and Videos
Topic: My First Attempts at Digital Photomicrography
Replies: 44
Views: 3484

Re: My First Attempts at Digital Photomicrography

No i do not at all. This too looks better than the original, however it is still lacking crispness. Maybe at this point the objectives do matter? That image was shot at 43x, so resolution should already be low correct? If so would, for example, an APO objective perform remarkably better?