MicrobeHunter.com Microscopy Forum

thanks Bob. As you note, I'm not able to use Kohler illumination because of a lack of a field diaphragm but I have ordered a cheap iris diaphragm to see if I can improvise a little with the light source. Last night I did try something that seemed to darken the background slightly. Although I don't h...

many thanks for the further help. Suphot - I will definitely try going through those steps to work out where the apparent light leak is coming from. Amscope do produce a dark field condenser and I did actually purchase one. However, frustratingly it is slightly too wide to fit into the condenser rin...

thanks for the further help and advice. Microbob - the grey background isn't an exposure issue unfortunately as I'm see it through the eyepieces as well as in the photo. I'm quite experienced in terms of photography and post processing so can create a darker background using the camera/software but ...

thanks John. I have the same problem whether I use the 4x or the 10x objectives. I'm not yet sure how you post photos but the following page on Amazon shows a number of images of my microscope including the stage and condenser https://www.amazon.co.uk/AmScope-T490B-3M-Veterinary-Trinocular-Microscop...

Many thanks John and also for the welcome. I was fairly confident that my stops were blocking the light but I've just tried using blu tack and I still have exactly the same problem - a grey background. The dark field effect is working i.e. the subject is illuminated by side lighting but I cannot get...

Hello, I'm relatively new to microscopy and am using an Amscope T490B compound microscope. I'm an experienced macro photographer (http://www.mattcolephotography.co.uk) and so my main interest is in photo microscopy but I've got a lot to learn when it comes to microscopy. I've been experimenting with...