Search found 967 matches

by hkv
Sun Apr 05, 2015 12:38 pm
Forum: Miscellaneous
Topic: Measuring Worth
Replies: 4
Views: 1812

Re: Measuring Worth

Yes, and that corredponds pretty well to what you have to pay for a new rotary microtome today.
by hkv
Sat Apr 04, 2015 10:52 am
Forum: Microscopy accessories
Topic: American Optical Microtome Model 810
Replies: 14
Views: 5295

Re: American Optical Microtome Model 810

The 810, 815, and 820 where available at the same time. The 810 being the simplest of the three, but also relatively light and compact compared to its bigger cousins. The knife you need is the American Optical 940. It is 110 mm wide. You can of course fit a longer knife as well, but then it will sti...
by hkv
Tue Mar 31, 2015 7:19 pm
Forum: My microscope
Topic: New arrival direct from Moscow
Replies: 32
Views: 8079

Re: New arrival direct from Moscow

Congrats on your new scope. Great feeling to unpack and assemble. Looks like it is in great condition.
by hkv
Sun Mar 22, 2015 10:06 pm
Forum: Pictures and Videos
Topic: Wing part of a house fly
Replies: 3
Views: 904

Re: Wing part of a house fly

I think this was a 10x objective. The condenser was an Olympus U-UCDB.
by hkv
Sun Mar 22, 2015 9:47 pm
Forum: Pictures and Videos
Topic: Plant histology -first attempt
Replies: 16
Views: 3202

Re: Plant histology -first attempt

Stunning results! Regarding the 0.02 micrometer cuts. That would equal to 20 nano meters. That is smaller than 1/10th of the wavelength of light. What type of knife edge would you need for these cuts? The edge of the blade would have to be very thin the the edge would not be optically resolvable in ...
by hkv
Sun Mar 22, 2015 9:36 pm
Forum: Pictures and Videos
Topic: Wing part of a house fly
Replies: 3
Views: 904

Wing part of a house fly

In "Darkfield". Not really, but I realized if I put the rotating DIC condenser between two positions the frame of the DIC prism blocks some of the light cone and a dark field like condition occurs. Not designed that way, but still an interesting side effect. https://www.dropbox.com/s/2rj7njsh20ilcve...
by hkv
Tue Mar 17, 2015 9:48 pm
Forum: Collecting microscopes and slides
Topic: New, but still old stuff
Replies: 11
Views: 3775

Re: New, but still old stuff

Peter wrote: Especially if you never use them.
Peter.
You are right. I WILL use the Reichert 820. :D
by hkv
Mon Mar 16, 2015 8:17 pm
Forum: Collecting microscopes and slides
Topic: New, but still old stuff
Replies: 11
Views: 3775

Re: New, but still old stuff

Hi HKV, Use them, they may be beautiful but they were designed and built as working tools (that's my opinion anyway). My microtome has two cams which are operated using ball and socket joints the sockets are plastic, the plastic material perished leaving the microtome inoperable until I made new pl...
by hkv
Tue Mar 10, 2015 9:32 pm
Forum: Collecting microscopes and slides
Topic: New, but still old stuff
Replies: 11
Views: 3775

New, but still old stuff

I am a sucker for old stuff in like-new conditions. Sharing with you a few of my recent purchases. Bu accident I found a pristine absolutely mint condition Reichert microscope. Not a scratch and with the manual and paper documentation in new condition. I don't think it has ever been used. Impossible...
by hkv
Tue Mar 10, 2015 8:38 pm
Forum: My microscope
Topic: A Few of my Modern Microscopes
Replies: 13
Views: 4710

Re: A Few of my Modern Microscopes

Thank you all! Yes, the Few which I use regularly. I don't have a round number of how many I have, just a Few hundred , but only 8-12 which I use regularly. The rest are shelf queen restorations of 19th and early 20th century microscopes. A few hundred :shock: :o How did you manage the WAF factor? ...
by hkv
Tue Mar 03, 2015 8:50 pm
Forum: Pictures and Videos
Topic: Pencil wood-shavings
Replies: 21
Views: 3368

Re: Pencil wood-shavings

Ha Ha, I love these! Also love the ingenuity behind producing the slices. This must be the cheapest Microtome available on the market. :P
by hkv
Tue Feb 24, 2015 8:46 pm
Forum: Camera systems and imaging
Topic: Photoshop E9 is unbelievably good at stitching!
Replies: 10
Views: 3635

Re: Photoshop E9 is unbelievably good at stitching!

Great! I need to try this out myself!
by hkv
Mon Feb 09, 2015 10:17 pm
Forum: Pictures and Videos
Topic: Midge Larva head with black eyes and sharp teeth
Replies: 4
Views: 1175

Re: Worm head with black eyes and sharp teeth

gekko wrote:Very nice! Could this be an insect larva?
You are right. It seems to be a Midge Larva. I have corrected the title of the thread. Sorry.

Thanks for your comments!
by hkv
Mon Feb 09, 2015 7:23 pm
Forum: Pictures and Videos
Topic: Antenna section of a Locust
Replies: 5
Views: 1437

Re: Antenna section of a Locust

Thanks all for the kind comments. Yes, DIC is great. I have it installed all the time more or less. No need to stain anymore since DIC seems to get contrast even for bacterias. Great technique. However, fluorescence illumination still needs proper staining. DIC will not solve that.
by hkv
Sun Feb 08, 2015 11:49 pm
Forum: Pictures and Videos
Topic: Cyanobactérie
Replies: 5
Views: 1353

Re: Cyanobactérie

Very nice! I need to look for those this summer! 8-)
by hkv
Sun Feb 08, 2015 11:34 pm
Forum: Pictures and Videos
Topic: Midge Larva head with black eyes and sharp teeth
Replies: 4
Views: 1175

Midge Larva head with black eyes and sharp teeth

DIC, 20x, stack of 29

Image
by hkv
Sun Feb 08, 2015 11:30 pm
Forum: Pictures and Videos
Topic: Antenna section of a Locust
Replies: 5
Views: 1437

Antenna section of a Locust

DIC, 20x, stack of 2

Image
by hkv
Wed Feb 04, 2015 8:24 pm
Forum: Pictures and Videos
Topic: Filamentous algae
Replies: 7
Views: 1615

Re: Filamentous algae

Great images! I need to start using my fluorescence equipment as well. Never used it yet...
by hkv
Wed Feb 04, 2015 8:05 pm
Forum: Pictures and Videos
Topic: Water creature with eggs
Replies: 6
Views: 1420

Re: Water creature with eggs

Thank you all :) Manfred, you have a point. It is wild life! Still, learning Lightroom is the next thing I'll have to do. I am sure Lightroom has similar features as Elements.
by hkv
Tue Feb 03, 2015 10:32 pm
Forum: Pictures and Videos
Topic: Caterpillar, larva of butterfly
Replies: 2
Views: 964

Caterpillar, larva of butterfly

Caterpillar, Butterfly larva tissue section. Sort of boring subject, but I liked the colorful patterns. From prepared slide. 10x, Brightfield.

Image
by hkv
Tue Feb 03, 2015 9:52 pm
Forum: Pictures and Videos
Topic: Water creature with eggs
Replies: 6
Views: 1420

Water creature with eggs

Water creature with eggs (water flea?) from prepared slide. Dark Field, 10x. No stacking.
I struggled for an hour to clean up the background in Lightroom 5, but I gave up. I need more training...

Image
by hkv
Tue Feb 03, 2015 7:14 pm
Forum: Pictures and Videos
Topic: Diatom (another)
Replies: 7
Views: 1491

Re: Diatom (another)

I like the last one, which almost look like a school book illustration. Looks like a painting almost. Nice effect. Is that achieved in the post processing or a "DIC effect"? I have not experienced such effect on my DIC setup (yet)
by hkv
Mon Feb 02, 2015 9:51 pm
Forum: Pictures and Videos
Topic: Hair on a fly's leg
Replies: 8
Views: 1823

Re: Hair on a fly's leg

Manfred wrote:Great, did you mazerate the speciman (perhaps KOH?), because it`s very clear.
I wish I remembered. This slide was prepared 30 years ago when I had my first microscope. I have then had a 30 year break before I picked up this hobby again.
by hkv
Mon Feb 02, 2015 5:32 pm
Forum: Pictures and Videos
Topic: Jacinthe corolle
Replies: 5
Views: 1255

Re: Jacinthe corolle

Wonderful images!
by hkv
Mon Feb 02, 2015 12:34 am
Forum: Pictures and Videos
Topic: Diatoms from a prepared slide of historical material
Replies: 6
Views: 1398

Re: Diatoms from a prepared slide of historical material

The last one is great! Somehow diatoms look nice on a black background!
by hkv
Mon Feb 02, 2015 12:30 am
Forum: Pictures and Videos
Topic: Hair on a fly's leg
Replies: 8
Views: 1823

Re: Hair on a fly's leg

Olympus UplanFl40x Phase, however, phase plate was not installed, so it is a regular bright field image.
by hkv
Mon Feb 02, 2015 12:24 am
Forum: Pictures and Videos
Topic: Diatom, part 2
Replies: 5
Views: 1164

Diatom, part 2

Another one in DIC, 40x objective (PlanFluorite). 2 images stacked.

Image

Image
by hkv
Sun Feb 01, 2015 5:08 pm
Forum: Pictures and Videos
Topic: Hair on a fly's leg
Replies: 8
Views: 1823

Hair on a fly's leg

This fly needs his legs shaved ;)

Image
by hkv
Sun Feb 01, 2015 4:59 pm
Forum: Pictures and Videos
Topic: Oniscus
Replies: 7
Views: 1534

Re: Oniscus

Where can one buy Calcofluor? I need some. Great result!
by hkv
Sun Feb 01, 2015 4:22 pm
Forum: My microscope
Topic: My setup
Replies: 7
Views: 3679

Re: My setup

Thanks! Yes it is a great scope with almost endless options and configuration. My goal is to have it complete with all options listed in the brochure. I have collected parts for it for the last two years from all over the world. The confocal option might be out of range. I saw one on ebay, used, but...