Search found 107 matches

by SunshineLW
Wed Apr 04, 2018 1:03 pm
Forum: Pictures and Videos
Topic: Tissue cytology of masses on my dog
Replies: 9
Views: 910

Re: Tissue cytology of masses on my dog

Lipomas are very common in older dogs and are estimated to occur in ~9% of dogs and ~3% of cats. They are almost always benign and it is very unusual for them to cause detrimental effects to the animal. I have piece of mind knowing that the mass on my dog's belly is most likely a lipoma. I say "most...
by SunshineLW
Wed Apr 04, 2018 11:44 am
Forum: Identification help
Topic: Larval Crab?
Replies: 2
Views: 919

Re: Larval Crab?

VERY COOL! Just add some color and:
by SunshineLW
Wed Apr 04, 2018 10:31 am
Forum: Pictures and Videos
Topic: Tissue cytology of masses on my dog
Replies: 9
Views: 910

Tissue cytology of masses on my dog

I took these samples from my dog last night. They are from two different masses that have been growing over the course of several weeks. Currently, I'm consulting with veterinary oncologists for a preliminary diagnosis. I'm confidant that the first mass is a lipoma. My guess for the second mass is p...
by SunshineLW
Sat Mar 31, 2018 8:36 am
Forum: Pictures and Videos
Topic: Baby flukes!
Replies: 10
Views: 1114

Re: Baby flukes!

I just watched a video of these guys on Youtube and it seems the wider end is the head end, although they do have flexibility and they sort of ooz about like an amoeba to some extent. You are correct. The wider end is the anterior. Also, two additional cool facts about miracidia: (1) Route of infec...
by SunshineLW
Sat Mar 31, 2018 8:27 am
Forum: Pictures and Videos
Topic: Stink bug hatching
Replies: 13
Views: 1385

Re: Stink bug hatching

VERY COOL! Thanks for sharing, Johann!
by SunshineLW
Thu Mar 15, 2018 4:00 pm
Forum: Identification help
Topic: Help! Sample take from chameleons eye (and infested fecal smear provided)
Replies: 4
Views: 1399

Re: Help! Sample take from chameleons eye (and infested fecal smear provided)

I would assume there are known methods for diagnosing roundworms in the eyes? Any specialist vets, that could advise you? Someone on a chameleon forum? As far as I am aware, all parasitic helminths of the eye are large enough to be seen grossly: Thelazia sp. is one of the few species of nematodes c...
by SunshineLW
Thu Mar 15, 2018 2:16 am
Forum: Identification help
Topic: Not sure, Schistosoma?
Replies: 27
Views: 4114

Re: Not sure, Schistosoma?

I read that the best time to find them is in mid-summer. So I'm curious when you happened to find yours? I think it has something to do with their lifecycle. You are correct! It has EVERYTHING to do with the life cycle, which is magnificent! The eggs are released in the feces of the definitive host...
by SunshineLW
Wed Mar 14, 2018 12:50 pm
Forum: Camera systems and imaging
Topic: Amscope 18 Megapixel (18MP) USB3.0 Digital Camera to Olympus BH-2
Replies: 24
Views: 5434

Re: Amscope 18 Megapixel (18MP) USB3.0 Digital Camera to Olympus BH-2

[\I note that the 'higher resolution copy' is 2400x1800 pixels, which is not the resolution of the camera. It would be very interesting to see some crops from the centre and corner of a full resolution single image of something boring, like a stage micrometer. Your stacked image is superb, but perh...
by SunshineLW
Wed Mar 14, 2018 12:17 pm
Forum: Camera systems and imaging
Topic: Amscope 18 Megapixel (18MP) USB3.0 Digital Camera to Olympus BH-2
Replies: 24
Views: 5434

Re: Amscope 18 Megapixel (18MP) USB3.0 Digital Camera to Olympus BH-2

If the 60x oil objective is affordable, and as optically good as your 100x, then yes; I would be inclined to stick with your current camera setup ... It's obviously effective and convenient. Sweet! Thank you for validating my assumption. I'm playing a guessing game right now, so any approval/ criti...
by SunshineLW
Wed Mar 14, 2018 12:07 pm
Forum: Camera systems and imaging
Topic: Amscope 18 Megapixel (18MP) USB3.0 Digital Camera to Olympus BH-2
Replies: 24
Views: 5434

Re: Amscope 18 Megapixel (18MP) USB3.0 Digital Camera to Olympus BH-2

Awesome scope and pics. I should have given them a higher offer :( I saw your setup and it puts mine to shame! Don't even play! lol, but thanks for the comment. Also, I believe the SPlanApo 40x is shot. I have cleaned the exterior, distal lens and I am unable to bring it into focus. Also, when atte...
by SunshineLW
Tue Mar 13, 2018 1:41 pm
Forum: Camera systems and imaging
Topic: Amscope 18 Megapixel (18MP) USB3.0 Digital Camera to Olympus BH-2
Replies: 24
Views: 5434

Re: Amscope 18 Megapixel (18MP) USB3.0 Digital Camera to Olympus BH-2

Thank you for your help zzffnn. I do not plan to build a DIC scope until I have more time to dedicate to learning the details. I desire PlanApo objectives because I took thousands of images using Plan objectives and the color aberrations haunt me in my dreams.
by SunshineLW
Tue Mar 13, 2018 1:21 pm
Forum: Camera systems and imaging
Topic: Amscope 18 Megapixel (18MP) USB3.0 Digital Camera to Olympus BH-2
Replies: 24
Views: 5434

Re: Amscope 18 Megapixel (18MP) USB3.0 Digital Camera to Olympus BH-2

If yes, you may be able to use a visual compensating 10x eyepiece (WHK10L?) with camera lens (around 60mm full frame equivalent) + a DSLR/mirrorless, and get camera image almost matching visual view field. I plan on imaging very few unstained specimens, so this does seem like a lot of work. The maj...
by SunshineLW
Tue Mar 13, 2018 12:36 pm
Forum: Camera systems and imaging
Topic: Amscope 18 Megapixel (18MP) USB3.0 Digital Camera to Olympus BH-2
Replies: 24
Views: 5434

Re: Amscope 18 Megapixel (18MP) USB3.0 Digital Camera to Olympus BH-2

Crater Eddie wrote:You have a great scope there, and your camera seems to be doing a fine job.
Thanks a lot for the kind words and encouragement. I'll definitely keep this thread up-to-date with any tweaks/ modifications in the setup.
by SunshineLW
Tue Mar 13, 2018 12:14 pm
Forum: Camera systems and imaging
Topic: Amscope 18 Megapixel (18MP) USB3.0 Digital Camera to Olympus BH-2
Replies: 24
Views: 5434

Re: Amscope 18 Megapixel (18MP) USB3.0 Digital Camera to Olympus BH-2

Crater Eddie wrote:...its just something that most of us learn to live with.
Do you think it could be corrected using this amscope reduction lens?: http://www.amscope.com/0-35x-c-mount-ca ... copes.html
by SunshineLW
Tue Mar 13, 2018 12:07 pm
Forum: Camera systems and imaging
Topic: Amscope Digital Camera to Olympus BH-2
Replies: 11
Views: 2905

Re: Amscope Digital Camera to Olympus BH-2

billbillt wrote:Please do share your work with us!!...

Here is a link to the updated thread: viewtopic.php?f=9&t=5853&p=52323#p52323
by SunshineLW
Tue Mar 13, 2018 12:03 pm
Forum: Camera systems and imaging
Topic: Amscope 18 Megapixel (18MP) USB3.0 Digital Camera to Olympus BH-2
Replies: 24
Views: 5434

Re: Amscope 18 Megapixel (18MP) USB3.0 Digital Camera to Olympus BH-2

Presumably the image field is significantly smaller than the visual field Hey Michael! Thanks for the comment. You are CORRECT! Thank you for mentioning this because it is something I desperately crave to correct. Currently, the Amscope camera is attached to the Amscope FMA050 fixed microscope adap...
by SunshineLW
Tue Mar 13, 2018 11:23 am
Forum: Pictures and Videos
Topic: Loa-Loa sp. microfilaria (Nematode) and a neutrophil with a background of RBCs
Replies: 2
Views: 487

Loa-Loa sp. microfilaria (Nematode) and a neutrophil with a background of RBCs

Loa-Loa sp. microfilaria (posterior end) and a neutrophil with a background of red blood cells: https://flic.kr/p/FvEaPp This image was taken using an Amscope 18 Megapixel (18MP) USB3.0 Digital Camera inserted directly into the Olympus BH-2 trinocular head. It was taken through an Olympus 100x SPlan...
by SunshineLW
Tue Mar 13, 2018 11:16 am
Forum: Camera systems and imaging
Topic: Amscope 18 Megapixel (18MP) USB3.0 Digital Camera to Olympus BH-2
Replies: 24
Views: 5434

Amscope 18 Megapixel (18MP) USB3.0 Digital Camera to Olympus BH-2

Thank you to those of you who have advised me in the recent purchase of my first compound microscope. Here is a preliminary image taken using the "Amscope 18 Megapixel (18MP) USB3.0 Digital Camera" inserted directly into the Olympus BH-2 trinocular head (without the use of additional camera accessor...
by SunshineLW
Sun Mar 11, 2018 4:01 pm
Forum: Identification help
Topic: Can anyone tell me what this is?
Replies: 12
Views: 1675

Re: Can anyone tell me what this is?

It could be a flatworm; however, it is no flatworm of mammals. If it is, it is one not mentioned in any modern textbooks.
by SunshineLW
Fri Mar 09, 2018 5:28 pm
Forum: Camera systems and imaging
Topic: Amscope Digital Camera to Olympus BH-2
Replies: 11
Views: 2905

Re: Amscope Digital Camera to Olympus BH-2

Okay, I think I understand now. I can remove the built-in lens from the Amscope camera and replace it with the U-PMTVC. If using a DSLR, I would only need the NFK 2.5 (plus a way to attach/position the lens of the DSLR above the NFK 2.5). When I have everything set up, I will: 1. Image through the t...
by SunshineLW
Fri Mar 09, 2018 1:45 pm
Forum: Camera systems and imaging
Topic: Amscope Digital Camera to Olympus BH-2
Replies: 11
Views: 2905

Re: Amscope Digital Camera to Olympus BH-2

You should be able to operate this way, though I think the Olympus optics will give you better color correction. YES! This is EXACTLY what I was looking for! Thank you so much for the images and clarification. I will probably follow exactly in your footsteps. If I ever decided to purchase one, coul...
by SunshineLW
Fri Mar 09, 2018 1:33 pm
Forum: My microscope
Topic: Latest Addition Olympus SZH
Replies: 6
Views: 2538

Re: Latest Addition Olympus SZH

Awesome setup! I'm drooling
by SunshineLW
Fri Mar 09, 2018 12:08 pm
Forum: Identification help
Topic: Not sure, Schistosoma?
Replies: 27
Views: 4114

Re: Not sure, Schistosoma?

Octane80 wrote:It was in a urine sample.
It has to be an artifact. I am not aware of anything natural or pathologic appearing as such in the urine.
by SunshineLW
Fri Mar 09, 2018 11:09 am
Forum: Identification help
Topic: Not sure, Schistosoma?
Replies: 27
Views: 4114

Re: Not sure, Schistosoma?

Sorry, I wasn't being clear back when I posted that -- I was referring to the larval stage when I asked that. I forget the name, but the stage after they leave the snail or mollusc host and have the divided tail. Ah, I see! You were referring to cercaria. They share very little resemblance to the a...
by SunshineLW
Fri Mar 09, 2018 11:03 am
Forum: Identification help
Topic: Neutrophil?
Replies: 7
Views: 2176

Re: Neutrophil?

Without question, this is by no means a neutrophil, no matter the environment.
by SunshineLW
Fri Mar 09, 2018 10:56 am
Forum: Identification help
Topic: ID help needed. Parasites?
Replies: 5
Views: 2261

Re: ID help needed. Parasites?

This is most likely NOT Giardia . These protozoa are ~10-20 micrometers in size and transparent without staining; therefore, you need ~1,000x magnification in order to properly identify without special staining (i.e., immunofluorescence). However, the last image (i.e., the image containing the two, ...
by SunshineLW
Fri Mar 09, 2018 10:42 am
Forum: Identification help
Topic: What Is This:
Replies: 10
Views: 2804

Re: What Is This:

Was thinking something in the order of trachelophyllum tachyblastum. Single shot picture taken at 200x magnification with a little camera zoom. Microscope bottom light. Sample collected from my pond. This is a shot in the dark, but it appears to be the palpi of a fly (or some other insect). Check o...
by SunshineLW
Fri Mar 09, 2018 10:27 am
Forum: Identification help
Topic: Blood Smear Identification Help
Replies: 9
Views: 2604

Re: Blood Smear Identification Help

These are, without a doubt, echinocytes (aka., crenated cells). This is a common artifact in properly-prepared blood smears of many mammalian species, especially swine and equine. It occurs when red blood cells (RBCs) are exposed to a hypertonic extracellular fluid environment (relative to the norma...
by SunshineLW
Fri Mar 09, 2018 10:10 am
Forum: Identification help
Topic: Any1 recognize that organizms ?
Replies: 6
Views: 2016

Re: Any1 recognize that organizms ?

Nothing in any of those images are helminths (worms); they all appear to be imposters.
by SunshineLW
Fri Mar 09, 2018 9:59 am
Forum: Identification help
Topic: What is that worms in blood ?
Replies: 3
Views: 1447

Re: What is that worms in blood ?

I agree with the latter comments in that this does not appear to be a hematoparasite. Assuming that this blood sample was taken from a peripheral vessel (rather than a great vessel or visceral vessel, such as the pulmonary arteries), you would not expect to see a nematode that large. Blood-borne nem...