Search found 144 matches

by mnmyco
Tue Oct 06, 2020 1:45 am
Forum: Illumination Techniques
Topic: Darkfield Adapter for Whole Slide Imaging
Replies: 13
Views: 1665

Re: Darkfield Adapter for Whole Slide Imaging

Maybe try using sodium silicate solution (water glass) to fill in the gaps between the led and the slide, though you left lose the rotation bit. You can also chart the wires with a thin layer of it to insulate them. I cannot remember, but adding some aluminum may make the sodium silicate have a high...
by mnmyco
Tue Oct 06, 2020 12:38 am
Forum: Microscopy accessories
Topic: AO part 2070
Replies: 3
Views: 337

AO part 2070

Hello Everyone,

Does anyone on know, with evidence/certainty that the vertical illuminator (part 2070) will work for a series 20 or 120? I am pretty sure it is meant for a series 10 DIC. Looking at apo here...

Thanks,

MNMyco
by mnmyco
Tue May 12, 2020 4:45 am
Forum: Microscopes and optics
Topic: (AO 20 series)Aligning the light path
Replies: 2
Views: 601

(AO 20 series)Aligning the light path

I wasn’t paying much attenion to what I was doing and loosed all three screws holding the nose piece in position on a AO series 20. Anyone have any advice on this. I wish I had a cross insert for the eye pieces. I have a cross Zeus slide, but even so in my opinion I should have a cross in the on the...
by mnmyco
Tue May 12, 2020 4:34 am
Forum: Home-made microscope adaptations
Topic: AO-Reichert 1973A condenser DIY help request
Replies: 14
Views: 6232

Re: AO-Reichert 1973A condenser DIY help request

No problem. I still cannot find the missing lens from the condenser I have. I keep my eye out though.
by mnmyco
Fri May 01, 2020 5:35 am
Forum: Microscopes and optics
Topic: A permanent grease for sealed microscope components: Krytox
Replies: 7
Views: 1695

Re: A permanent grease for sealed microscope components: Krytox

Pure PTFE is a solid until ~327 C and is practically insoluble in everything. Anything claiming to be pure PTFE and is not a solid is not pure PFTE and has a carrier in it.
by mnmyco
Wed Feb 19, 2020 2:17 am
Forum: Specimens, samples and slides
Topic: Better choice of mounting medium for phase contrast
Replies: 8
Views: 3650

Re: Better choice of mounting medium for phase contrast

Try a saturated solution of calcium chloride and/or saturated solution of potassium acetate. The CaCl2 won’t dry out. Glycerol saturated with sodium borate (20 mule team borax) is nice but annoying to make. Pure wintergreen oil. Water saturated with thymol, which is insanely fragrant and not pleasan...
by mnmyco
Fri Dec 20, 2019 2:00 pm
Forum: Off Topic
Topic: There Are Thousands of Tardigrades on the Moon. Now What?..
Replies: 9
Views: 12058

Re: There Are Thousands of Tardigrades on the Moon. Now What?..

Without a nice atmosphere to help block the suns radiation, I don’t think could survive any reasonable amount of time.


MNMyco
by mnmyco
Tue Dec 10, 2019 2:40 pm
Forum: Pictures and Videos
Topic: Dancing worms in on of my pond tanks probably Tubifex
Replies: 4
Views: 963

Re: Dancing worms in on of my pond tanks

I love those little guys. A tiny little vibration and they hide though. I have found them to be somewhat seasonal if you leave them near an exterior window.

MNMyco
by mnmyco
Tue Nov 12, 2019 6:59 pm
Forum: Microscopy accessories
Topic: AO parfocal camera tubes, formerly on ebay.
Replies: 1
Views: 2053

Re: AO parfocal camera tubes, formerly on ebay.

Good to know. I was going to buy one soon.
by mnmyco
Tue Nov 12, 2019 2:29 am
Forum: Microscopes and optics
Topic: AO 120 Binocular prism repair
Replies: 1
Views: 898

AO 120 Binocular prism repair

I just recently picked up a nice AO 120, complete with the external lamp housing, the lamp socket, filters, transformer, and binocular head. Oh, and the 180 eye pieces. For $80 I think it was a bit of a steal. Anyway, I am not sure if it was in the shipping during cold weather or the reason the scop...
by mnmyco
Tue Nov 12, 2019 2:19 am
Forum: Microscopes and optics
Topic: Ortholux tube lens
Replies: 5
Views: 1606

Re: Ortholux tube lens

I believe the answer can be found in one of my first posts, which was the same question as yours.

http://www.microbehunter.com/microscop ... f=5&t=6549

MNMyco
by mnmyco
Fri Nov 08, 2019 7:38 am
Forum: Specimens, samples and slides
Topic: can i wash and reuse bacterial heat set slides?
Replies: 5
Views: 3009

Re: can i wash and reuse bacterial heat set slides?

For high school, I would say use a mix of vinegar and original formula dawn dish detergent. When I ran a microbiology and mycology lab, we would put slides in a 2 liter container with the vinegar/dawn mix and let it sit until full. We then cleaned them all and let them dry. The stronger the acetic a...
by mnmyco
Wed Nov 06, 2019 5:30 am
Forum: Resources (online, books etc.)
Topic: AO MicroStar 120
Replies: 2
Views: 2353

Re: AO MicroStar 120

Thanks. I checked his sight, but missed it. Just goes to show I am worthless when I have a migraine.

MNMyco
by mnmyco
Wed Nov 06, 2019 2:16 am
Forum: Resources (online, books etc.)
Topic: AO MicroStar 120
Replies: 2
Views: 2353

AO MicroStar 120

I am trying to get a hold of the series 120 manual. I have the 110 and vertical illumination 110/120 but cannot seem to find a 120 manual.

Thanks,

MNMyco
by mnmyco
Tue Nov 05, 2019 11:21 pm
Forum: Microscopy accessories
Topic: AO beam splitter/vertical illuminator
Replies: 5
Views: 2804

Re: AO beam splitter/vertical illuminator

Well, I know that there are biological series 10 with epi lighting setups that look like the one in the link you sent. Not the whole thing, just the light itself. If there is such an add-on to a series 20 or 10 I hope to some day get it. Off topic, perhaps, but does anyone recognize the extra piece ...
by mnmyco
Tue Nov 05, 2019 7:26 am
Forum: Microscopy accessories
Topic: AO beam splitter/vertical illuminator
Replies: 5
Views: 2804

Re: AO beam splitter/vertical illuminator

I thought it looked odd compared to their other vertical units, but all the ones I have seen actual photos of and not just drawing were fluorescence lighting systems.

Thanks
by mnmyco
Tue Nov 05, 2019 5:44 am
Forum: Microscopy accessories
Topic: AO beam splitter/vertical illuminator
Replies: 5
Views: 2804

AO beam splitter/vertical illuminator

I was thinking of bidding on this eBay item number 153667503727. It is an AO 1111-3 beam splitter/vertical illuminator. However, it looks like it might have a damaged lens in one of the photos and I don’t know the light you would use for the vertical illumination. Any ideas guys.

Thanks
by mnmyco
Tue Nov 05, 2019 3:34 am
Forum: About the website, the magazine and this forum
Topic: Vetting newcomers
Replies: 15
Views: 6746

Re: Vetting newcomers

Tricky-one there Wes, seems at first-glance a good idea..... in principle certainly. It seems perhaps that the mechanism, in terms of how quickly the personae non gratae are removed, banned etc. Perhaps an automatic (i.e. without Oliver's attention required) removal or maybe suspension pending revi...
by mnmyco
Mon Nov 04, 2019 8:44 pm
Forum: About the website, the magazine and this forum
Topic: Vetting newcomers
Replies: 15
Views: 6746

Re: Vetting newcomers

Or at the very least a quick way to report posts and/or users that are spammers.
by mnmyco
Sun Nov 03, 2019 10:48 pm
Forum: Identification help
Topic: Is this a slime mold?
Replies: 8
Views: 4899

Re: Is this a slime mold?

It is definitely a slime mold and not a Tremella species or any other fungus.
by mnmyco
Thu Oct 31, 2019 1:56 am
Forum: Pictures and Videos
Topic: Ciliate in Methylcellulose
Replies: 13
Views: 3133

Re: Ciliate in Methylcellulose

I have asked this company for a sample, but I also had it shipped to my work, at a university. Their samples are 500 g.

https://www.cpkelco.com/products/cellulose-gum-cmc/

I have CMC and gellan gum from them. Works well.

MNMynco
by mnmyco
Wed Oct 30, 2019 5:26 pm
Forum: Pictures and Videos
Topic: Ciliate in Methylcellulose
Replies: 13
Views: 3133

Re: Ciliate in Methylcellulose

I wonder if happened to get a very small particle methylcellulose that did not dissolve and is trying to clear it.
by mnmyco
Thu Oct 24, 2019 8:08 pm
Forum: Beginner's corner
Topic: Am I doing something wrong?
Replies: 9
Views: 2601

Re: Am I doing something wrong?

You can read the field guide to bacteria for help on what can be useful when dealing with bacteria. I have a lot of experience in teaching undergraduate microbiology labs. The only thing we use microscopes for is to check microbe shape (which even its good scopes at 100x can be misleading or inconcl...
by mnmyco
Mon Oct 21, 2019 9:55 pm
Forum: Microscopes and optics
Topic: Sorry, but need a quick answer
Replies: 1
Views: 1109

Sorry, but need a quick answer

Does anyone know if the nosepiece, binocular, and halogen lamp socket from an AO microstar that is in their white, square time period (so like series 410 or so look) will fit a series 20? I got an amazing private offer considering what is on the microscope. I only have 30 hours or so. Again sorry fo...
by mnmyco
Mon Oct 21, 2019 3:13 am
Forum: Specimens, samples and slides
Topic: Disposing of stains from home microscopy lab
Replies: 8
Views: 3619

Re: Disposing of stains from home microscopy lab

No, it does. There was a paper published on it. If I can find it again I will post a link. I refuse to stop using it. I mean I’ve had to regularly work with KCN and NaCN. If I can protect myself from exposure to those I think I can for EtBr as well.
by mnmyco
Sun Oct 20, 2019 2:42 am
Forum: Specimens, samples and slides
Topic: Disposing of stains from home microscopy lab
Replies: 8
Views: 3619

Re: Disposing of stains from home microscopy lab

Oxidizing can produce even worse chemicals. For instance, bleach and ethidium bromide makes much worse chemicals than ethidium bromide. California actually allows 9 ng/L of ethidium bromide in the normal sewer system in some cities. I doubt you produce enough to cause any harm however you dispose of...
by mnmyco
Fri Oct 18, 2019 10:49 pm
Forum: Microscopes and optics
Topic: First Surface Mirror?
Replies: 13
Views: 4334

Re: First Surface Mirror?

There are two types of hard drive disks. Some are made of aluminum and others are made of ceramic. Seems most people that cut them use a jewelers saw and some sort of cutting fluid I believe. Maybe some day I will give making a DIY water jet cutter. I know it has been done.

MNMyco
by mnmyco
Fri Oct 18, 2019 4:51 pm
Forum: Home-made microscope adaptations
Topic: Fixed TL and Infinity Inter-company Compatibly
Replies: 3
Views: 2846

Re: Fixed TL and Infinity Inter-company Compatibly

You have a point, but I do not see that as a reason to not generate such a list. I was thinking of things beyond just fixed vs infinite TL. A good example is the use of 70x LOMO WI lenses without the water immersion. Or knowing if I could screw a Zeus condenser into a AO condenser and still have pha...
by mnmyco
Fri Oct 18, 2019 4:47 pm
Forum: Microscopes and optics
Topic: First Surface Mirror?
Replies: 13
Views: 4334

Re: First Surface Mirror?

Good point. Do you have any suggestions for a good way to test that? Especially if it does not involve cutting the disks?

MNMyco
by mnmyco
Fri Oct 18, 2019 5:37 am
Forum: Illumination Techniques
Topic: Relief Phase Contrast Microscopy - Article by Prof J. Piper in Microscopy and Analysis, vol 21, 2007.
Replies: 15
Views: 5262

Re: Relief Phase Contrast Microscopy - Article by Prof J. Piper in Microscopy and Analysis, vol 21, 2007.

I have ended up with two turret condenser bodies for my AO series 10 and 20 microscope. Once I get them both in working order (need another trinocular and series 20 lamp cord and socket) I will try to create a much more controlled test of this “relief phase contrast”.

MNMyco