Search found 144 matches

by mnmyco
Wed Dec 05, 2018 4:38 pm
Forum: Pictures and Videos
Topic: Hoffman Modulation Contrast
Replies: 14
Views: 2610

Re: Hoffman Modulation Contrast

Hoffman avoids the costly prisms of DIC, that is the main bonus. The book, Light and Video Microscopy by Randy Wayne has a great chapter on it. It only uses a polarizing filter, retardation film, and slits in filters to to convert phase differences in the specimen into visible differences. A slit fi...
by mnmyco
Mon Dec 03, 2018 9:24 pm
Forum: Pictures and Videos
Topic: more Biddulphia pulchella
Replies: 16
Views: 3096

Re: more Biddulphia pulchella

Well, I know nothing about said Biddy, but this is a the fine tome that I use to unpick an understand the binomial names in my Botanical pursuits - I find it very useful indeed to understand the meanings that are so often considered prosaic although personally I find a certain charm 'in there' also...
by mnmyco
Mon Dec 03, 2018 4:58 pm
Forum: Pictures and Videos
Topic: Scone Surprise - YUK
Replies: 13
Views: 1852

Re: Scone Surprise - YUK

wstenberg wrote:Doesn't all bread have yeast all over it? I've never had a scone; not sure exactly what it is, but it sounds rather bread-like...
Yeast will look like large bacteria, and will not survive the cooking process. Also, not all bread has yeast.

mnmyco
by mnmyco
Mon Dec 03, 2018 4:56 pm
Forum: Beginner's corner
Topic: Care and feeding of protozoa zoo?
Replies: 3
Views: 1693

Re: Care and feeding of protozoa zoo?

Duck weed needs lots of nitrogen. I use some limestone or clam shells to help buffer the water's pH, and add either soil or a tiny amount of fertilizer. I good general book that I like is: A Sourcebook for the Biological Sciences. https://www.amazon.com/Sourcebook-Biological-Sciences-Evelyn-Morholt/...
by mnmyco
Thu Nov 29, 2018 4:57 pm
Forum: Pictures and Videos
Topic: Amoeba (arcella?) 'walking'
Replies: 14
Views: 2687

Re: Amoeba (arcella?) 'walking'

That is awesome.
by mnmyco
Thu Nov 29, 2018 2:36 pm
Forum: Pictures and Videos
Topic: Scone Surprise - YUK
Replies: 13
Views: 1852

Re: Scone Surprise - YUK

That is a species of Penicillium. Personally, I prefer fuchsine in lactic acid. No phenol, the lactic acid is a fixative and thick enough to take days to dry out, and has a different RI than the stained fungal tissues further enhancing contrast.

mnmyco
by mnmyco
Fri Nov 16, 2018 2:31 pm
Forum: Pictures and Videos
Topic: Another Moss Dissection
Replies: 23
Views: 3463

Re: Another Moss Dissection

Stunning work! from what I scantily read about mosses, I thought that they do not possess conducting tubes... As for the images, they very nicely and clearly convey the botanical information! I notice that the leaves of your moss sample are quite large - 5mm long or so? BTW: Your "expensive" forcep...
by mnmyco
Mon Nov 12, 2018 5:02 pm
Forum: My microscope
Topic: Chinese microscope manufacturers overview
Replies: 72
Views: 29949

Re: Chinese microscope manufacturers review

Good to know more brands, but using "review" in the title is a bit confusing. I had expected more than an overview of the companies.

mnmyco
by mnmyco
Mon Nov 12, 2018 3:13 pm
Forum: Identification help
Topic: Sporangium of fungi?
Replies: 2
Views: 2153

Re: Sporangium of fungi?

It is a member of the family Mucoraceae, likely a Mucor , Rhizomucor , or Rhizopus . It is an immature sporangium though, notice how thick the wall is and the lack of obvious spores inside. You need the entire structure, intact, including the point of origin on the hypha and preferably, a mature spe...
by mnmyco
Wed Nov 07, 2018 5:15 pm
Forum: Microscopes and optics
Topic: What is this?
Replies: 6
Views: 1466

Re: What is this?

Ah. Now that you say that it is easy to recognize.

mnmyco
by mnmyco
Tue Nov 06, 2018 7:58 pm
Forum: Collecting microscopes and slides
Topic: A hidden gem of superior optics - Wolfe/Kyowa
Replies: 12
Views: 8650

Re: A hidden gem of superior optics - Wolfe/Kyowa

Sounds good. I used a tiny 1.3 mm drill bit and a hand push drill to drill out the majority of the piece. A trade sounds good. Just let me know what parts you want to swap. You might need to re-tap the hole for the knob in the condenser. I tried to be careful, but I probably brushed up against it wi...
by mnmyco
Tue Nov 06, 2018 6:58 pm
Forum: Microscopes and optics
Topic: What is this?
Replies: 6
Views: 1466

What is this?

Can anyone figure out what this thing is?

https://www.shopgoodwill.com/Item/59846887
by mnmyco
Fri Oct 26, 2018 3:51 pm
Forum: Camera systems and imaging
Topic: Connecting Canon DSLR to Leitz trinocular head with all-Leitz components
Replies: 52
Views: 17853

Re: Connecting Canon DSLR to Leitz trinocular head with all-Leitz components

I have a canon 60d and an ortholux with a trinocular head. I use this:

https://www.amazon.com/OMAX-Microscope- ... B01D914LT2

It works in any eyepiece so I do not need all the other attachments. It is a built in 2x lens.

mnmyco
by mnmyco
Wed Oct 24, 2018 6:52 pm
Forum: Pictures and Videos
Topic: Oamaru New Zealand Fossil Diatoms
Replies: 15
Views: 2365

Re: Oamaru New Zealand Fossil Diatoms

Ah, I see. I was curious if you had one of the special rigs that move the camera a known distance. I have yet to try just putting my camera in the port and turning the fine focus for stacking. Good to know that you can do that and get quality images.

mnmyco
by mnmyco
Wed Oct 24, 2018 3:18 pm
Forum: Pictures and Videos
Topic: Oamaru New Zealand Fossil Diatoms
Replies: 15
Views: 2365

Re: Oamaru New Zealand Fossil Diatoms

What do you use to control the focusing while taking the images for stacking? Do you use a normal canon lens for photography or do you use a specialty adapter lens, such as this: https://www.amazon.com/OMAX-Microscope-Adapter-Canon-D-SLR/dp/B01D914LT2/ref=sr_1_3?ie=UTF8&qid=1539802448&sr=8-3&keyword...
by mnmyco
Wed Oct 24, 2018 3:04 pm
Forum: Identification help
Topic: Helizoa
Replies: 6
Views: 2297

Re: Helizoa

The last photo is, I believe, a fern spore that has opened. You can actually watch them pop open under a microscope. Pretty fun. http://www.microscopy-uk.org.uk/mag/imgmay00/sporangia1.jpg Video (quality is low though) https://www.youtube.com/watch?v=Q6b_GxnTgjg You just stick them on a slide in a t...
by mnmyco
Wed Oct 24, 2018 2:52 pm
Forum: Microscopes and optics
Topic: Questions about Wild Heerbrugg M11 microscope eyepieces and light source
Replies: 13
Views: 3314

Re: Questions about Wild Heerbrugg M11 microscope eyepieces and light source

Hi, I actually am a mycologist. I would strongly recommend buying a objective that is around 50x to 70x. On a Leica DM2500 (if I recall correctly) I was most found of the 63x objective. It provided a much better image of spores than the 40x and still did not need oil. You can use reticles in Huygens...
by mnmyco
Wed Oct 24, 2018 1:24 pm
Forum: Microscopy accessories
Topic: Forgive me if I try to manipulate you, a little!!
Replies: 23
Views: 8755

Re: Forgive me if I try to manipulate you, a little!!

The "Ms" appear to be of different widths and the details at the tops of the "Ms" and the "Ns" do not appear consistent to me. Still it is pretty perfect looking overall. Either way, it is a nice machine. Any idea what the mycology lab was using it for? Professional interest on my part. mnMYCO I wa...
by mnmyco
Wed Oct 24, 2018 1:47 am
Forum: Microscopy accessories
Topic: Forgive me if I try to manipulate you, a little!!
Replies: 23
Views: 8755

Re: Forgive me if I try to manipulate you, a little!!

The "Ms" appear to be of different widths and the details at the tops of the "Ms" and the "Ns" do not appear consistent to me. Still it is pretty perfect looking overall. Either way, it is a nice machine. Any idea what the mycology lab was using it for? Professional interest on my part.

mnMYCO
by mnmyco
Wed Oct 24, 2018 1:37 am
Forum: Microscopes and optics
Topic: AO Objective Identification
Replies: 5
Views: 2167

Re: AO Objective Identification

Ah, what I thought were most likely catalog numbers were the phase annulus number. Kind of annoying that I searched the AO catalogs for those series and could not find matching catalog numbers. Thanks for the info. I will hold on to them for now, but I am more likely to sell them than anything else....
by mnmyco
Tue Oct 23, 2018 8:56 pm
Forum: Microscopes and optics
Topic: identify a microscope DIC or Phase Contrast
Replies: 13
Views: 3122

Re: identify a microscope DIC or Phase Contrast

You can get results that give a 3 dimensional appearance ( similar to DIC) to the specimen by using oblique techniques. oblique techniques can get good nice picture? vs phase contrast which is batter? Depends. I do like oblique and phase. Still DIC will give better results for technical analysis, b...
by mnmyco
Mon Oct 22, 2018 6:29 pm
Forum: Microscopes and optics
Topic: AO Objective Identification
Replies: 5
Views: 2167

AO Objective Identification

Hi, I have a set of AO objectives that I am working on getting scope for (maybe), but so far I have not been able to match what I believe are the catalog numbers on the objectives to online resources. I started at PS Neeley's website all abount AO and had no luck, and I had no luck with internet sea...
by mnmyco
Sun Oct 21, 2018 11:32 pm
Forum: Illumination Techniques
Topic: Darkfield photography
Replies: 28
Views: 12264

Re: Darkfield photography

@billbillt Kohler also removes the appearance of dust on parts of the optical system and removes unnecessary light. So, it does more than just remove the filament image. If I recall correctly, the only issue with LED I am aware of is that you cannot use a simple rheostat to control brightness withou...
by mnmyco
Fri Oct 19, 2018 3:01 am
Forum: Microscopes and optics
Topic: Ortholux tube lens (1.25x)
Replies: 6
Views: 1764

Re: Ortholux tube lens (1.25x)

I did not think it was a lens to correct for infinity, but what is it for? Just increasing image magnification?
by mnmyco
Thu Oct 18, 2018 6:16 pm
Forum: Digital processing
Topic: Measureing Software
Replies: 8
Views: 10980

Re: Measureing Software

@Brad: I have used ImageJ (not FiJi) a lot in the past, including programming of Macros, and did not encounter any particular problems, other than some inconvenience because of the logic of the macro commands. I ran it on a Windows 7, I7 CPU-64bit-6GB memory PC. Perhaps it depends on certain ImageJ...
by mnmyco
Thu Oct 18, 2018 2:40 pm
Forum: Home-made microscope adaptations
Topic: DIY Phase contrast
Replies: 10
Views: 5436

Re: DIY Phase contrast

Awesome work. Hope the write-up come soon. I am very interested in the DIY type stuff.
by mnmyco
Thu Oct 18, 2018 2:36 pm
Forum: Microscopes and optics
Topic: Ortholux tube lens (1.25x)
Replies: 6
Views: 1764

Ortholux tube lens (1.25x)

I have been trying to find out exactly what a lens in my ortholux is doing. It is a 1.25x lens that is in between the objectives and eye pieces. This is an old black enamel ortholux with 170 objectives. Is this the lens people refer to when talking about creating infinity correction when using non-i...
by mnmyco
Thu Oct 18, 2018 1:47 pm
Forum: Microscopes and optics
Topic: disassembly of Carl Zeiss Jena Amplival phase contrast condenser
Replies: 11
Views: 2662

Re: disassembly of Carl Zeiss Jena Amplival phase contrast condenser

Some advice was provided at the workshop that is different from that some of that offered on this and other fora. E.g., optical components such as objectives are cleaned primarily with 70% isopropyl alcohol and Q-tips cotton swabs (that brand only). That is in line with the Zeiss in house cleaning ...
by mnmyco
Thu Oct 18, 2018 1:32 pm
Forum: Digital processing
Topic: Measureing Software
Replies: 8
Views: 10980

Re: Measureing Software

I would highly recommend ImageJ. ImageJ is a completely free image editing and manipulation program made with US government funding. It is used in countless scientific publications and has great modules etc that people have written for it. The whole purpose is to analyze images for scientific invest...
by mnmyco
Wed Oct 17, 2018 6:54 pm
Forum: Illumination Techniques
Topic: Darkfield photography
Replies: 28
Views: 12264

Re: Darkfield photography

I think we really need to know how you are attaching the camera first. I have used a Canon 60D dSLR and had no issues with dark field like this. I used an adapter that attaches to your camera like a normal lens and fits into eye pieces or C-mounts. $60 on amazon. Something like this, but I bought it...