Search found 100 matches

by lperepol
Sat Dec 29, 2018 1:33 pm
Forum: Beginner's corner
Topic: What is a good specimen or procedure to test quality of phase contrast?
Replies: 3
Views: 1157

What is a good specimen or procedure to test quality of phase contrast?

Looking for some good specimens, pictures, procedures to compare against and quantify the quality of phase contrast.
by lperepol
Sat Dec 29, 2018 4:15 am
Forum: Identification help
Topic: Started out as a sauerkraut smear on agar - 14 days.
Replies: 2
Views: 1131

Started out as a sauerkraut smear on agar - 14 days.

I don't know what it is but looks cool. https://lh3.googleusercontent.com/EK0h9naiBhM1gAZRu2VBK903ac0fIoyD2E-08nYVIYcRR771QYlOBfASa6gXbkNf2kC-3fiy-sdqPETwtiBn7OLTnEPrfnP9vX5CPjhAvM0GZReklFbxj6UivQ-RE6QxL1MtDJmv89duwf1NKT4r231viSArBkL5TrvSN44u5bRNTV2qKArr9_hkugXnIPlbKB-0Sc6agpsIOMHYKXLUhv0nMdjVVtUtHj...
by lperepol
Fri Dec 28, 2018 7:55 pm
Forum: Miscellaneous
Topic: Mounting needle
Replies: 7
Views: 3271

Re: Mounting needle

A biology dissection kit has some nice tools. There are a few of them on Amazon. And not too pricey. I got this one. It is working well for me: https://www.amazon.com/Anatomy-Biology-Dissection-Scalpel-Blades/dp/B07DH9MDS3/ref=sr_1_1_sspa?ie=UTF8&qid=1546026764&sr=8-1-spons&keywords=biology+dissecti...
by lperepol
Fri Dec 28, 2018 2:56 pm
Forum: Pictures and Videos
Topic: Practising microscopy techniques on sauerkraut bacteria.
Replies: 1
Views: 859

Sauerkraut bacteria goes hi-tech

https://www.ncbi.nlm.nih.gov/pmc/articl ... dMIKEb68IY
:D

Does not mention when or how Sauerkraut goes sideways though.
by lperepol
Fri Dec 28, 2018 9:05 am
Forum: Pictures and Videos
Topic: Practising microscopy techniques on sauerkraut bacteria.
Replies: 1
Views: 859

Practising microscopy techniques on sauerkraut bacteria.

Should posts like these be posted in the sandbox? Practising microscopy techniques on bacteria. Heat fixing, oil immersion, methylene blue stain (staining). Image one seems like I was looking at yeast cells. Over time the bacteria seem to have gotten smaller? And seems like I could focus for a bette...
by lperepol
Fri Dec 28, 2018 2:47 am
Forum: Resources (online, books etc.)
Topic: van Egmond masks
Replies: 2
Views: 2961

Re: van Egmond masks

Thanks, 75RR the pdf masks are cool!!

I need to spend more time reading howtos and techniques:
http://www.microbehunter.com/oblique-illumination/
by lperepol
Thu Dec 27, 2018 9:51 pm
Forum: Pictures and Videos
Topic: Some Pond Life In Southern Central British Columbia (Kootenay Area)
Replies: 6
Views: 1245

Re: Some Pond Life In Southern Central British Columbia (Kootenay Area)

Thank you I was up most of the night finding distinct critters. Look like I also found a leech or two. I am new to microscopy and have a very limited vocabulary. Is Oblique the same as phase contrast? Does it require another gadget? I have phase contrast but images do not seem to appear as sharp -- ...
by lperepol
Thu Dec 27, 2018 6:11 am
Forum: Pictures and Videos
Topic: Some Pond Life In Southern Central British Columbia (Kootenay Area)
Replies: 6
Views: 1245

Re: Some Pond Life In Southern Central British Columbia (Kootenay Area)

Practice Focus Stacking with Picolay. Possible water mite. http://www.microscopy-uk.org.uk/index.html?http://www.microscopy-uk.org.uk/pond/ https://lh3.googleusercontent.com/7rZG-YpHbCEwq6YFU81t3yHm2EHPZ4LGt6PjUa2hiQ9KdYaOiSOh6kYZMKmiHX7tosBVhEzXIIkPdoBmRvNd-ZvdCZZqkpyu8HdK2fYqLYjk7BxT4EUk_bFA3xfIYB...
by lperepol
Thu Dec 27, 2018 6:10 am
Forum: Pictures and Videos
Topic: Some Pond Life In Southern Central British Columbia (Kootenay Area)
Replies: 6
Views: 1245

Some Pond Life In Southern Central British Columbia (Kootenay Area)

A drop of pond water in a well slide and waiting for some water to evaporate the critters easier to see and find.
by lperepol
Tue Dec 25, 2018 7:46 am
Forum: Beginner's corner
Topic: What are some of the most popular stains (please include wet mount media also)used in Microscopy?
Replies: 3
Views: 1215

What are some of the most popular stains (please include wet mount media also)used in Microscopy?

I am taking a poll on the most commonly used stains in microscopy. I'll summarize after a few posts. Popular Science has a similar article -- that might need to be revisited. Here is what I have on my list including wet mount media: 1) Bismarck Brown Y 2) Eosin Y 3) India Ink 4) Iodine Solution 5) J...
by lperepol
Mon Dec 24, 2018 11:34 pm
Forum: Specimens, samples and slides
Topic: Comparing 8 Stains on Moldy Carrots
Replies: 3
Views: 1680

Sclerotia No Stain and Bismarck Brown Y stain A few more to go

Thank you Hobbyst46 and mrsonchus for your feedback. I appreciate tips!!! The Bismarck Brown stain looks suspiciously rather cellular. I need practice looking at and dissecting stuff.
Using buffered glycerol for a wet mount.

by lperepol
Mon Dec 24, 2018 9:42 pm
Forum: Specimens, samples and slides
Topic: Comparing 8 Stains on Moldy Carrots
Replies: 3
Views: 1680

Comparing 8 Stains on Moldy Carrots

Stains 1) Bismarck Brown Y 2) Eosin Y 3) India Ink 4) Iodine Solution, 0.1N (0.1M) 5) Janus Green B  6) Methylene Blue 7) Neutral Red 8) Red Food Coloring I would like to get my hands on Lactophenol. I have not been able to find a source in Canada or one that will ship to Canada. Some stains are dif...
by lperepol
Mon Dec 24, 2018 6:59 pm
Forum: Identification help
Topic: Any ideas of what is going on with fungus and iodine?
Replies: 5
Views: 1389

Re: Any ideas of what is going on with fungus and iodine?

Thank you for your time and explanation Hobbyst46!! I have a lot of fungus and a few more stains. I'll do a visual comparison of various stains at time t0 and tn and see what happens. It took a while until I noticed the behaviour of the iodine. I'll see how other stains fair. Something wetter than w...
by lperepol
Mon Dec 24, 2018 5:23 pm
Forum: Identification help
Topic: Any ideas of what is going on with fungus and iodine?
Replies: 5
Views: 1389

Re: Any ideas of what is going on with fungus and iodine?

I am assuming that there must be some sort of nifty esoteric explanation. :geek: Like what is being absorbed? The fungus is growing on carrots that were stored in a plastic bag. I kept the fungus growing for further investigation and to attempt a positive Id of the fungus. Sclerotia is a bad omen fo...
by lperepol
Mon Dec 24, 2018 5:00 pm
Forum: Identification help
Topic: Any ideas of what is going on with fungus and iodine?
Replies: 5
Views: 1389

Any ideas of what is going on with fungus and iodine?

Just wondering about what is happening. I place a drop of Iodine on a fungus (I think sclerotia) and the iodine drop appears to be clearing and the fungus is absorbing a colour. Is this some sort of a cation exchange? The iodine drop beads but something is being pulled out of the iodine. Tried a few...
by lperepol
Mon Dec 24, 2018 7:09 am
Forum: About the website, the magazine and this forum
Topic: Image from Google Photos?
Replies: 8
Views: 5429

Re: Image from Google Photos?

Sweet!! Thanks for the tip actinophrys!!!
So it's a Google thing. Hm, I thought I tried that? I right clicked on the image and selected copy image address.
One has to also set the privacy settings on Google Photos for sharing.
by lperepol
Mon Dec 24, 2018 12:14 am
Forum: Identification help
Topic: A Large Something Or Other In The Vermicompost Tea!
Replies: 1
Views: 1937

A Large Something Or Other In The Vermicompost Tea!

A critter in vermicompost tea after 8 days of aeration. Is it a Red Wiggler earthworm (Eisenia fetida) or a Nematode? From previous observations when I aerated I saw that Red Wigglers die off and float to the top then sink. I do not think a red wiggler can survive in compost tea. So do not think it ...
by lperepol
Sun Dec 23, 2018 10:22 pm
Forum: About the website, the magazine and this forum
Topic: Image from Google Photos?
Replies: 8
Views: 5429

Re: Image from Google Photos?

This image works fine in any browser but it has a very large file size and large dimensions: https://photos.google.com/share/AF1QipOpggw6Mi52Luq2xMp-6c6iPVXnr28LEufcT86Cg8VHqRGrWwvFl9Ij3IWOoB-srQ/photo/AF1QipPnaCEu2QEa0RKHTt0pTAZDEbvn5O9c3-e2YK7T?key=RktOSERkNllFRVhubmxxY3JFcS1mZVNBS2VWakV3 Even IE ...
by lperepol
Sun Dec 23, 2018 9:53 pm
Forum: Pictures and Videos
Topic: Vermicompost tea after 8 days of aeration.
Replies: 4
Views: 955

Re: Vermicompost tea after 8 days of aeration.

Thank you for the help 75RR!!
I'll order some concentration methylcellulose.
by lperepol
Sun Dec 23, 2018 9:40 pm
Forum: About the website, the magazine and this forum
Topic: Image from Google Photos?
Replies: 8
Views: 5429

Re: Image from Google Photos?

Posting the links will do. Facebook messes (scales, crops and compresses) with uploaded images. Google photos seems to handle large file sizes and image dimensions well. I'll start using Google more for lossless images.
by lperepol
Sun Dec 23, 2018 6:16 pm
Forum: About the website, the magazine and this forum
Topic: Image from Google Photos?
Replies: 8
Views: 5429

Re: Image from Google Photos?

That is what the HTML attributes are for to limit display sizes of incoming pictures. So users do not have to fiddle with the images themselves. The form should be able to fix this. They are just HTML links. The form is not storing the images. It is somewhat a form of unnecessary user harassment imp...
by lperepol
Sun Dec 23, 2018 5:31 pm
Forum: About the website, the magazine and this forum
Topic: Image from Google Photos?
Replies: 8
Views: 5429

Image from Google Photos?

Is there an image size limitation? Images display on google photos but when I post the link to the image on the form only the default thumbnail is displayed on the form. For exmaple: https://photos.google.com/share/AF1QipOpggw6Mi52Luq2xMp-6c6iPVXnr28LEufcT86Cg8VHqRGrWwvFl9Ij3IWOoB-srQ?key=RktOSERkNl...
by lperepol
Sun Dec 23, 2018 4:59 pm
Forum: Pictures and Videos
Topic: Vermicompost tea after 8 days of aeration.
Replies: 4
Views: 955

Re: Vermicompost tea after 8 days of aeration.

Even at 40x with no well slide, the organisms are just shadows shooting around.
What kind to the medium can they be mounted in that will dry with no air bubbles?
Would something like glycerol work?
Or just let them dry on a slide without a coverslip then apply coverslip after drop dries?
by lperepol
Sun Dec 23, 2018 2:39 pm
Forum: Pictures and Videos
Topic: Vermicompost tea after 8 days of aeration.
Replies: 4
Views: 955

Vermicompost tea after 8 days of aeration.



At 20X, a 40X Objective bends the coverslip on a concave slide.

Looks like a nematode survived 8 days of aeration at 15° to 20° c temperature.

Very small critters in there!
by lperepol
Sat Dec 22, 2018 1:00 pm
Forum: Pictures and Videos
Topic: A Nematode --> a possible Panagrolaimus
Replies: 1
Views: 767

Re: A Nematode --> a possible Panagrolaimus

Garden Nematodes
A partial cast of characters.
by lperepol
Thu Dec 20, 2018 2:03 am
Forum: Specimens, samples and slides
Topic: About Nematodes
Replies: 9
Views: 3188

Re: About Nematodes

I have found a way of estimating nematode populations, stain with iodine, let them die. take picture(s) and count on picture them. Graph paper (squared paper) as background can help. Now if I can get links to google photos to display as pictures. https://photos.google.com/share/AF1QipPRErPH4StqHFIlh...
by lperepol
Wed Dec 19, 2018 6:55 pm
Forum: Specimens, samples and slides
Topic: About Nematodes
Replies: 9
Views: 3188

I am wondering if there are stains that enhance different anatomical body parts of nematodes?

1) I am wondering if there are stains that enhance a nematode's tooth? 2) I am wondering if there are stains that enhance a nematode's stylet? 3) I am wondering if there are stains that enhance different anatomical body parts of a nematode? This would be to help in comparing nematode species/genus? ...