Search found 68 matches

by iconoclastica
Wed Mar 06, 2019 9:22 pm
Forum: Pictures and Videos
Topic: Metopus sp.
Replies: 1
Views: 1226

Re: Metopus sp.

#MeToo-pus?
by iconoclastica
Sun Mar 03, 2019 7:35 pm
Forum: Beginner's corner
Topic: Choosing ring light for Stereo Microscope
Replies: 26
Views: 8084

Re: Choosing ring light for Stereo Microscope

In the living-room I had led spots that were too blue. That was easily solved with one layer of amber gel. If your luminous output is sufficient, you can do such things.
by iconoclastica
Sun Mar 03, 2019 7:16 pm
Forum: Beginner's corner
Topic: Choosing ring light for Stereo Microscope
Replies: 26
Views: 8084

Re: Choosing ring light for Stereo Microscope

I don't know how to quantify the blue-ness of light, but here's a comparison. The same patch photographed under the led-ringlight and under a daylight photo-lamp:
daylight white balance
daylight white balance
patch.jpg (32.3 KiB) Viewed 7976 times
Note that the ringlight is several years old now and dominant led-types have changed.
by iconoclastica
Sun Mar 03, 2019 12:39 pm
Forum: Specimens, samples and slides
Topic: Bud Dissection - Mystery Contents
Replies: 11
Views: 3584

Re: Bud Dissection - Mystery Contents

Congrats, John :( Ffor this is what we know about fusarium: "...cause fusarium rot in plants. They affect the roots that then blacken and die. Next the whole plant wilts and dies. There is no remedy. All affected plants must be destroyed asap to pevent further contamination. The soil must be sterili...
by iconoclastica
Sun Mar 03, 2019 12:27 pm
Forum: Beginner's corner
Topic: Choosing ring light for Stereo Microscope
Replies: 26
Views: 8084

Re: Choosing ring light for Stereo Microscope

I quite agree with hobbyist46. Quite a long time ago I acquired a cold light source with the fibre-optic goosenex, mainly because I then thought it was the type of light one ought to use. I never liked it much, never enough light, always my hands in the way, ever noisy and hot. When I got my Mz7.5, ...
by iconoclastica
Fri Mar 01, 2019 10:45 am
Forum: Microscopes and optics
Topic: turning the barrel
Replies: 2
Views: 719

Re: turning the barrel

Very recognisable confusion ;) The sequence is well known to me indeed, but not always where I am within that sequence. O well, if it can't be done, it can't be done. I'll devise labeled sleeves then, for both the RFID sticker and human readable labels.
by iconoclastica
Thu Feb 28, 2019 11:13 pm
Forum: Microscopes and optics
Topic: turning the barrel
Replies: 2
Views: 719

turning the barrel

I notice that when mounted on a turret, the faces - i.e. the engravings with the magnification, N.A. etc - of the objectives do not all point an the same radial direction. I would ecpect them to look either outward or to the axis. So the position relative to the start of the thread is not constant a...
by iconoclastica
Tue Feb 19, 2019 9:49 am
Forum: Pictures and Videos
Topic: Rhabdonema adriaticum
Replies: 4
Views: 918

Re: Rhabdonema adriaticum

I would say it was a little square for a nematode. They tend to be longer and thinner. ;)
Duh, could see that :D

Rhabditis, Rhabdolaima: I was fooled by the -nema-part.
by iconoclastica
Mon Feb 18, 2019 10:23 pm
Forum: Pictures and Videos
Topic: Rhabdonema adriaticum
Replies: 4
Views: 918

Re: Rhabdonema adriaticum

Rhabdonema, isn't that a nematode?
by iconoclastica
Mon Feb 18, 2019 10:19 pm
Forum: Collecting microscopes and slides
Topic: Make Your Own Micropaleontology Slides..
Replies: 2
Views: 1713

Re: Make Your Own Micropaleontology Slides..

There's some clever ideas in that paper, thank you for the lead. I might try it for storing Isoetes megaspores. I think with the aid of a lasercutter, the construction will be precise and easier.
by iconoclastica
Thu Feb 14, 2019 6:50 pm
Forum: Beginner's corner
Topic: What alternatives are there to match the eye piece field of view with a camera's field of view.
Replies: 5
Views: 2630

Re: What alternatives are there to match the eye piece field of view with a camera's field of view.

What do you mean by 'match'? If I take that litterally, you are after a circular image, such as the top-left one of the bottom six in Charlie's document. The image projection on the sensor should then be small to see a full round black vignette. If on the other hand you mean 'about the same extent' ...
by iconoclastica
Mon Feb 11, 2019 6:03 pm
Forum: Beginner's corner
Topic: Is a eyepiece and an objective enough for a microscope ?
Replies: 19
Views: 5108

Re: Is a eyepiece and an objective enough for a microscope ?

A microscope is indeed a combination of an objective and an eyepiece. So, now we can do without those condensers? Honestly, you can buy a used starter's microscope for less than 100 euro. It may be simple, but probably better and possibly cheaperthan anything you can make yourself. Then you can tur...
by iconoclastica
Mon Feb 11, 2019 5:50 pm
Forum: Beginner's corner
Topic: Is a eyepiece and an objective enough for a microscope ?
Replies: 19
Views: 5108

Re: Is a eyepiece and an objective enough for a microscope ?

How about plywood and a scroll saw. Even cheaper. You can probably find the plywood in a dumpster and borrow the tools from a tool library. That's not my experience. I never get it exactly right that way, and doing and redoing it doesn't make it more economical. That said, I wouldn't choose for the...
by iconoclastica
Fri Feb 08, 2019 5:08 pm
Forum: Microscopes and optics
Topic: What's this scale?
Replies: 9
Views: 1324

Re: What's this scale?

That was clear from the beginning, Fan. It seems I misunderstood Pete.

Wim
by iconoclastica
Fri Feb 08, 2019 4:30 pm
Forum: Microscopes and optics
Topic: What's this scale?
Replies: 9
Views: 1324

Re: What's this scale?

The top mark is for normal 160mm brightfield and 210mm brightfiled/darkfiled (but not DIC) use with an accessory lamp head. The "R" mark drops the stage down to allow the insertion of Reflected DIC prisms. The "T" mark drops the even stage further to allow the insertion of a normal DIC slider attac...
by iconoclastica
Fri Feb 08, 2019 4:26 pm
Forum: Microscopes and optics
Topic: What's this scale?
Replies: 9
Views: 1324

Re: What's this scale?

It is as I surmised, the DIC unit comes between the frame and the turret. This adds some 2cm that the stage should be lowered. http://www.photomacrography.net/forum/userpix/3106_Optiphot_2_1.jpg (photo from photomacrography.net ) It's unlikely that I'll ever do DIC, but I do like to know the functio...
by iconoclastica
Fri Feb 08, 2019 3:42 pm
Forum: Microscopes and optics
Topic: What's this scale?
Replies: 9
Views: 1324

Re: What's this scale?

All DIC-related, could be. But how would that work, does the DIC-unit sit between the turret and the frame, thus lowering the objectives by some 20mm? It's briefly mentioned in the version two manual that the dot should be aligned with the arrow/triangle. Seemingy, the Optiphot is a 1/2 hybrid in it...
by iconoclastica
Fri Feb 08, 2019 2:14 pm
Forum: Microscopes and optics
Topic: What's this scale?
Replies: 9
Views: 1324

What's this scale?

I am slowly turning my Labophot into a customized Optophot. Now I note a difference in the carrier mechanism of the stage, that I don't find explained in the manual(s). Moving with the focusing, there's a scale (R-DIC-T) and above that an arrow and a dot on the other side. A screw allows for adjusme...
by iconoclastica
Mon Feb 04, 2019 11:37 pm
Forum: Illumination Techniques
Topic: A DIY LED ring for darkfield
Replies: 76
Views: 24905

Re: A DIY LED ring for darkfield

I had not expected wonders from the tubes, but it was just too easy to try. I am not sure at all I can do the leds, but I won't know until I try. We'll see. But no pcb: for one thing I can't make them, and neither do I think it's a useful approach here, for the leds will sit on a narrow (1-3mm) edge...
by iconoclastica
Mon Feb 04, 2019 7:57 pm
Forum: Illumination Techniques
Topic: A DIY LED ring for darkfield
Replies: 76
Views: 24905

Re: A DIY LED ring for darkfield

Instead of LEDs, it might also be feasable to use the CCFL tubes that are used in lcd-displays. These are very thin fluorescent tubes. Just tried that. I inherited a pair of 8x10cm displays, the lcd already removed. In the frame is a U-shaped light tube and its high-voltage converter is on board. I...
by iconoclastica
Sun Jan 27, 2019 2:44 pm
Forum: Illumination Techniques
Topic: A DIY LED ring for darkfield
Replies: 76
Views: 24905

Re: A DIY LED ring for darkfield

Instead of LEDs, it might also be feasable to use the CCFL tubes that are used in lcd-displays. These are very thin fluorescent tubes.
by iconoclastica
Sun Jan 27, 2019 2:39 pm
Forum: Digital processing
Topic: Stacking focus consistency
Replies: 11
Views: 5271

Re: Stacking focus consistency

I find 1-2µm quite big steps, but of course all depend on your intended magnification. I quite agree on your stand on software, but i have been a software engineer all my life. Not everyone feels the same about it. Triggering the camera is easy: with an optocoupler yout short the pc remote cable for...
by iconoclastica
Sat Jan 26, 2019 11:25 pm
Forum: Digital processing
Topic: Stacking focus consistency
Replies: 11
Views: 5271

Re: Stacking focus consistency

On the microscope, I make focus steps by eye judgement. I watch where the focus is now, where the next details will likely appear, and turn the wheel until I see them. Of course, I scout the subject before starting, so I know where to look. This works quite well in all magnifications (4x-100x). Step...
by iconoclastica
Fri Jan 25, 2019 8:13 pm
Forum: Illumination Techniques
Topic: Nikon epi-illuminator - now what?
Replies: 12
Views: 4613

Re: Nikon epi-illuminator - now what?

Does this require special objectives? Or will it work with, say, Phase Contrast objectives? Epi-illumination in itself does not require special objectives. Only, often it is used with thick specimens that cannot be viewed in trans-illumination, such as mineral samples. More often than not it is poi...
by iconoclastica
Thu Jan 24, 2019 6:48 pm
Forum: Illumination Techniques
Topic: Nikon epi-illuminator - now what?
Replies: 12
Views: 4613

Re: Nikon epi-illuminator - now what?

I am quite satisfied with the results indeed. Today, I removed the metal bottom of the outer tube that had provided me with an unsollicited extra aperture. This resulted in some 30% more light output, if the scale of a photoresistor module is anywhere near linear. And also more freedom to move the l...
by iconoclastica
Thu Jan 24, 2019 6:20 pm
Forum: Microscopes and optics
Topic: Optiphot, direct projection?
Replies: 11
Views: 2743

Re: Optiphot, direct projection?

Chris, I use the discussion head to project the primary image onto the sensor directly. I use a dovetail adapter, m42 extension tube as (coarse) spacer, a helicoid for fine focusing, and finally an M42-EOS adapter. The image easily covers APS-C and is wider than seen through the eye-pieces. It may b...
by iconoclastica
Thu Jan 24, 2019 1:03 pm
Forum: About the website, the magazine and this forum
Topic: uploaded images gone after a few days
Replies: 3
Views: 6976

Re: uploaded images gone after a few days

Hi Michael

I understand your reaction. I thought I might get an url like the [img]-tag defines one. In edit-mode, all I see is [attachment=0]stoma.jpg[attachment]. But when I returned, without saving, the image was there. Must be a caching issue then?

Wim
by iconoclastica
Thu Jan 24, 2019 12:04 pm
Forum: About the website, the magazine and this forum
Topic: uploaded images gone after a few days
Replies: 3
Views: 6976

uploaded images gone after a few days

I find myself frequently reading old discussions, both on this forum as on others. Much knowledge has been accumulated here, even though sometimes it's hard to find. I note though, that often illustrations have gone missing. I presume this is inevitable when the pictures are stored on other servers ...
by iconoclastica
Wed Jan 23, 2019 1:34 pm
Forum: Illumination Techniques
Topic: Nikon epi-illuminator - now what?
Replies: 12
Views: 4613

Re: Nikon epi-illuminator - now what?

This morning I made a few technical tests with the illuminator. I realized that there ought to be a conjugal image at the position of the aperture diaphragm [AD]. (As an aside: could anyone explain why in reflective köhler the field aperture comes after the AD?). I tried to open the illuminator to p...
by iconoclastica
Wed Jan 23, 2019 12:51 pm
Forum: Illumination Techniques
Topic: Nikon epi-illuminator - now what?
Replies: 12
Views: 4613

Re: Nikon epi-illuminator - now what?

Approx what exposure do you need? I just made a test exposure (details follow) of a piece of white acrylic and let the camera make it mid grey +1. This gave me 1/6th@ISO100, aperture diaphragm wide open, field diaphragm set to fit field, LED half power. For comparison, in transmission lighting with...