Search found 479 matches

by Wes
Sun Mar 08, 2020 8:59 pm
Forum: Beginner's corner
Topic: Spectra services
Replies: 5
Views: 3890

Re: Spectra services

daruosha wrote:
Sun Mar 08, 2020 5:50 pm
rs6000 wrote:
Sun Mar 08, 2020 4:24 pm
what are these guys smoking???
Perhaps Immersion Oil. :D
:lol: :lol: :lol:
rs6000 wrote:
Sun Mar 08, 2020 4:24 pm
though IMHO the last straw was there asking 3800.00$ for a 360Deg rotatable stage for a WL
There is a price reduction on that item, its only $2,661.40
by Wes
Sun Mar 08, 2020 9:52 am
Forum: My microscope
Topic: My Zeiss Universal Project
Replies: 30
Views: 11789

Re: My Zeiss Universal Project

Very cool thread Microworldofgems! The paint job is great and I find your prism repair inspiring. Would Canada balsam work for gluing the prism wedges? I noticed you removed the optovar from the tube head. I had the impression once its off its nearly impossible to get it back to its original position.
by Wes
Sat Mar 07, 2020 8:00 pm
Forum: Pictures and Videos
Topic: fungus
Replies: 5
Views: 3327

Re: fungus

Great work as always sinabro. Always looking forward to your threads!
by Wes
Fri Mar 06, 2020 7:54 pm
Forum: Miscellaneous
Topic: Glossary of Ebay seller terms
Replies: 11
Views: 7388

Re: Glossary of Ebay seller terms

75RR wrote:
Fri Mar 06, 2020 7:49 pm
BramHuntingNematodes wrote:
Fri Mar 06, 2020 7:14 pm
I won an item recently I knew might be broken, found out it was definitely broken when I got it, and still think I got a pretty good deal.
The usefulness of door stops is underestimated ;)
I am currently sporting some very nice Planapo paperweights.
by Wes
Fri Mar 06, 2020 7:51 pm
Forum: Pictures and Videos
Topic: Diversity in one sample from aquarium
Replies: 20
Views: 6288

Re: Diversity in one sample from aquarium

Cool video mintakax, and an even nicer aquairum! My wife freaks out over the sight of a couple of baby jars filled with about 100 ml of dirty water, I'll have a hard time convincing her to get one of these bad boys :lol:
by Wes
Fri Mar 06, 2020 7:37 pm
Forum: Beginner's corner
Topic: Zeiss DIC- could this work?
Replies: 7
Views: 4374

Re: Zeiss DIC- could this work?

I'd make a guess and say no. I tried to use the dedicated prisms for the INKO condenser you posted in an epi-illumination setup and it did not work at all. Still, give it a try I'm curious to see what you come up.
by Wes
Tue Mar 03, 2020 7:33 pm
Forum: Microscopes and optics
Topic: Frustration!
Replies: 64
Views: 15781

Re: Frustration!

I got into microscopy as a hobby a few years ago, and, frankly, it's been mostly frustration for me since then. Almost all of this frustration has resulted from equipment issues. Ah, yes I hate when that happens. Buy an "excellent" photo eyepiece with no issues, has fungus...buy a "perfect" Planapo...
by Wes
Sat Feb 29, 2020 8:53 pm
Forum: Pictures and Videos
Topic: Windows
Replies: 3
Views: 1050

Re: Windows

wow, I'd hang that on my wall :D
by Wes
Sat Feb 29, 2020 3:00 pm
Forum: Pictures and Videos
Topic: Some living diatoms I
Replies: 4
Views: 1500

Re: Some living diatoms I

Very nice set!

Would you mind telling us what type of objectives you used for taking these shots.
by Wes
Thu Feb 27, 2020 6:22 pm
Forum: Miscellaneous
Topic: What is your favorite microscopy YouTube channel?
Replies: 10
Views: 3173

Re: What is your favorite microscopy YouTube channel?

In addition that what has been mentioned I quite like the following two: TheMicrobiology09 https://www.youtube.com/user/TheMicrobiology09 Craig Smith https://www.youtube.com/user/MrInsight1979 Its a shame they don't post anymore cause the quality is superb (Craig Smith recently sold his excellent Un...
by Wes
Wed Feb 26, 2020 11:23 pm
Forum: Pictures and Videos
Topic: House fly - beheaded and cleared in KOH (process explained)
Replies: 8
Views: 2532

Re: House fly - beheaded and cleared in KOH (process explained)

Thanks for the interesting description, I always enjoy reading how people prepare their samples.

Quite nice photo too, I wouldn't have come up with phase contrast for this particular subject but it really makes it stand out. Quite alien really. Nice one!
by Wes
Mon Feb 17, 2020 8:00 am
Forum: Pictures and Videos
Topic: Pine stem cross section
Replies: 10
Views: 2211

Re: Pine stem cross section

I agree with the members above me. This is a really good result!

Would be interesting to get some details on your sectioning technique as well as fixing, staining and mounting.
by Wes
Sat Feb 15, 2020 10:05 am
Forum: Pictures and Videos
Topic: Surirella gemma
Replies: 10
Views: 3583

Re: Surirella gemma

Excellent work 75RR. Really love the contrast and resolution here. The B/W fits quite well with the subject.
by Wes
Mon Feb 10, 2020 7:35 pm
Forum: Beginner's corner
Topic: DNA?
Replies: 7
Views: 3354

Re: DNA?

Wes - Your detailed response just leads me to do some online research into the process - just out of curiosity. My "lab" is one end of a dining room table. I lack knowledge and resources to go much beyond what I'll get from the kit. Mary I don't think you need a dedicated kit for isolating plant DN...
by Wes
Mon Feb 10, 2020 6:20 pm
Forum: Beginner's corner
Topic: DNA?
Replies: 7
Views: 3354

Re: DNA?

Recently I prepared a large amount of supercoiled plasmid DNA from E.coli and let it form DNA liquid crystals on a slide. For this you don't need high magnification at all. You can actually observe single DNA strands but first you need to label actively replicating cells with 5-halogenated uridine a...
by Wes
Mon Feb 10, 2020 8:40 am
Forum: Beginner's corner
Topic: First microscope for the-odin
Replies: 12
Views: 3805

Re: First microscope for the-odin

Wes, what fluorescent microscope would you recommend? I would probably go for this one: https://www.ebay.com/itm/T165875-Carl-Zeiss-Trinocular-Phase-Contrast-Microscope-w-Epi-Illuminator/333508383896?hash=item4da6a61498:g:32gAAOSwFWNePf-p&shqty=1&isGTR=1#shId It comes with no objectives and eyepiec...
by Wes
Sun Feb 09, 2020 6:18 pm
Forum: Pictures and Videos
Topic: Nitzschia sigmoidea
Replies: 10
Views: 3329

Re: Nitzschia sigmoidea

Beautiful work 75'

How do you do the stitching? I tried to load the files to be stacked via "File>Script>Load Files Into Stack" and then "Edit>Auto-Align Layers".
by Wes
Sat Feb 08, 2020 9:27 pm
Forum: Beginner's corner
Topic: First microscope for the-odin
Replies: 12
Views: 3805

Re: First microscope for the-odin

Hi Hobbyst46, I realized now I made a mistake in my post from a few months ago (its the same image). According to the metadata of all the confocal images I've posted the objective that was used is Plan-Apochromat 40x/1.4 Oil DIC M27 (I will correct this in my original post) The image is a z-stack 3D...
by Wes
Sat Feb 08, 2020 8:29 pm
Forum: Beginner's corner
Topic: First microscope for the-odin
Replies: 12
Views: 3805

Re: First microscope for the-odin

I've encountered consistently negative feedback regarding The ODIN. Contaminated cultures/media, expired antibiotics, water instead of DNA, enzymes shipped at room temperature and so on. I can recommend looking in a fluorescent microscope for genetic engineering. You could for example fuse GFP (gree...
by Wes
Mon Jan 27, 2020 3:38 pm
Forum: Pictures and Videos
Topic: chilomonas
Replies: 5
Views: 1078

Re: chilomonas

Spectacular quality. I really like that video, you can see the ejectosomes so clearly. I'd be very interested to see hi-res stills of the little critters ;)

Best regards
Wes
by Wes
Sun Jan 26, 2020 1:13 pm
Forum: Illumination Techniques
Topic: Making DIC Prisms
Replies: 14
Views: 4049

Re: Making DIC Prisms

Out of interest have you also considered making quartz wedge compensators? Add it to a 3d printed DIN 6x20mm holder and you have my money ;)
by Wes
Sat Jan 25, 2020 7:37 pm
Forum: Pictures and Videos
Topic: Stentor or vorticella?
Replies: 6
Views: 1233

Re: Stentor or vorticella?

I would go with Stentor as well. Just a minor heads up ... Many of us, and I include myself, use Vorticella which is a Genus, interchangeably with Peritrich which is the Order it belongs to. My point is that not every Peritrich is a Vorticella and not every ciliate that looks like a vorticella is o...
by Wes
Thu Jan 23, 2020 8:19 am
Forum: My microscope
Topic: Reichert Polyvar Microscope
Replies: 60
Views: 11099

Re: Reichert Polyvar Microscope

apochronaut wrote:
Mon Jan 13, 2020 12:06 pm
a severe temperature drop finishes the process
What would constitute a severe temperature drop?
by Wes
Wed Jan 22, 2020 11:27 pm
Forum: Pictures and Videos
Topic: Licmophora flabellata
Replies: 11
Views: 2043

Re: Licmophora flabellata

Amazing shot!

How did you fix the background noise in the image?
by Wes
Tue Jan 21, 2020 9:49 pm
Forum: Illumination Techniques
Topic: Interference contrast? Interphako or Jamin-Lebedeff or Mach-Zehnder
Replies: 3
Views: 1824

Re: Interference contrast? Interphako or Jamin-Lebedeff or Mach-Zehnder

Nice, I'm looking forward to seeing your work.

I saw the JL set, looks really cool but $5000 would leave a hole too large for my hobby budget.
by Wes
Tue Jan 21, 2020 9:40 pm
Forum: Microscopes and optics
Topic: petrographic microscope technique question for calcite DIC raw materials
Replies: 8
Views: 1092

Re: petrographic microscope technique question for calcite DIC raw materials

Given a block of calcite, what is the best way to accurately find its optical axis? Would it need to be polished to find that? Ideally it would be nice to shoot polarized colminated light through, motor an objective around to find the maximum output plane. I'm still vague on the optical axis aspect...
by Wes
Tue Jan 21, 2020 5:45 pm
Forum: Pictures and Videos
Topic: ciliate under a dic
Replies: 3
Views: 541

Re: ciliate under a dic

Nice video!

Mind telling us about your microscope, DIC setup and camera configuration?
by Wes
Mon Jan 20, 2020 11:20 pm
Forum: Illumination Techniques
Topic: Making DIC Prisms
Replies: 14
Views: 4049

Re: Making DIC Prisms

Great project! I was under the impression that making DIC prisms is extremely difficult hence the astronomical prices. Something along the lines of cutting slabs at an angle of 30 seconds. A lot of prisms have to produced to generate a few that are fit for purpose, these are then binned according to...
by Wes
Thu Jan 16, 2020 8:14 pm
Forum: Illumination Techniques
Topic: Interference contrast? Interphako or Jamin-Lebedeff or Mach-Zehnder
Replies: 3
Views: 1824

Re: Interference contrast? Interphako or Jamin-Lebedeff or Mach-Zehnder

I have a Jamin-Lebedeff condenser but no matched objectives and beam recombiners. Its a set of techniques I'm quite interested in learning more about and trying for myself but as you said these are extremely difficult to find. If its not too much I'd love to see your Jamin-Lebedeff set and some micr...
by Wes
Mon Jan 13, 2020 7:44 pm
Forum: Identification help
Topic: Diatom ID
Replies: 0
Views: 5414

Diatom ID

A freshwater diatom that measures 70x12 microns. The image is a stack of 13 photos. For some reason the bottom layer of scum in one of my pond cultures is riddled with diatom frustules.

Image