MicrobeHunter.com Microscopy Forum

Triceratium favus var quadrata


Stictodiscus californicus var areolata

Bruce Taylor wrote: ↑

Wed Dec 02, 2020 10:05 pm

As for the ID, my understanding is that is extremely difficult to distinguish most Mayorella species from Korotnevella, unless you have access to SEM.

Thanks Bruce! I once asked Ferry Siemensma about an amoeba I had made a video of and he just said Mayorella.

Amateur, everyone knows you should put in a pressure cooker and then bang it with a hammer.

https://www.ebay.de/itm/7-Fach-Wechsel- ... Swne1fx-HM

Found this while browsing ebay, last one I saw sold for a few hundred and I thought someone here might be interested.

It came with the microscope, its installed by default. The photo below shows you where its placed on a Universal stand. You might be able to source one at a reasonable price on Ebay (search for KG1 heat absorbing filter). That is interesting. Was going to put it on the field diaphragm housing filte...

Again no anti-UV measures but I do use a KG1 IR blocking filter. The microscope has one installed at the base between the source and field diaphragm. The lamp gets incredibly hot so I think its important to protect your eyes and optics (and specimens I guess) against potentially harmful IR radiatio...

Thanks 75'! That was dramatic! Was it at normal speed? Yes, the fluorescence videos are at normal speed. The DIC and phase are both at 4x. I take it you used Interference Filters with your 100w halogen lamp. Did you take any UV precautions or just made sure to use the camera as a go between? Correct...

Thanks Hobbyist! I think the phototoxicity of acridine orange is not uncommon among protists. Initially I was studying its effects on Paramecium, however it doesn't explode but contracts and releases vesicular contents in the cytoplasm but doesn't decompose like the amoeba. I used a 100 W halogen la...

Hi everyone, I was able to enrich a population of Mayorella (correct me if my identification is wrong) and decided to experiment with it. Here I present some videos showing what happens to the amoeba when it is stained with acridine orange and irradiated with 450-490 nm light. After a few seconds of...

No change when lowering the condenser and the warm up effect argue against a smudge on one of the conjugate planes. I'd still have a look at these though. Check out the binocular head too.

I think this is dirt on one (or more..) of a few conjugate planes. I recently cleaned those cause it drove me crazy too! It solved the problem.

Can you post a picture of your scope and also a view of its bottom/base plate?

Could be the Zeiss Standard 18 you're thinking of Wes - with interchangeable turrets and a 100 watt lamp? Just as a price comparison, one of these days I'll be selling a Zeiss 18 with full DIC, 100 watt source, Plan NeoFluars, and a trinocular head for substantially less than 2200 Euro equivalent. ...

Thank you! I found a zeiss axiolab A1 for a good price. The microscope is in a very very good state. It has 5 objectives ( 4 ph contrast). I might think of buying this and then change something as I need. What do you think? Thank you I appreciate What are the objectives? For the money you offer I w...

A potentially good source for quality research grade used microscopes is University surplus. Not what they intend to dump in the trash but what they want to get replace, to upgrade a laboratory. Perhaps in the school of medicine or life sciences. Even old confocal microscopes are being removed from...

LouiseScot wrote: ↑

Sat Nov 21, 2020 5:16 pm

Wes wrote: ↑

Wed Nov 04, 2020 6:55 pm

Btw I have a video showing the tip of a Spirogyra with lots of moving stuff including big chunks of cytoplasmic content getting transferred around. In case you are interested I can upload it to youtube.

Yes, please do!
Thanks
Louise

As you wish, sir!

Very clear view of Euplotes! I struggle to get a good view at these without resorting to large quantities of methylcellulose.

Nice, sharp video and interesting documentation. Does hydra even eat protists?

ImperatorRex wrote: ↑

Sat Nov 07, 2020 9:10 am

An old but very informative video that matches this topic and content:
https://av.tib.eu/media/18700

Thanks! There are also some very interesting videos of Vampyrella eating the chloroplasts of Spirogyra on this site.

Any thoughts on using one of these? Yeah, don't use any of these unless you want a fancy new paperweight? Find medical cotton, not the Qtip kind that has silica microparticles which will scratch your objective and get the purest water you can find (maybe set up a crude distillation setup for a few ...

This slot is for the 4x12 sliders, these are the older full metal sliders not the newer 6x20 plastic ones.

What microscope is that?

Should have shot with the 16x or 40x

Any thoughts on using one of these? Yeah, don't use any of these unless you want a fancy new paperweight? Find medical cotton, not the Qtip kind that has silica microparticles which will scratch your objective and get the purest water you can find (maybe set up a crude distillation setup for a few ...

I'd say its the Pleurosigma that photobombs the bacteria.

I like it though, nice shot! Taken with the Planapo 63/1,4?

This is a video of onion cells showing cyclosis (cytoplasmic streaming). You can see 2 classes of objects, big highly refractile bodies (storage granules I suspect) and if you pay close attention you can spot smaller bodies of lower contrast that appears somewhat more heterogenous in shape (visible ...

Absolutely not, you can't see single ribosomes not even polysomes (multiple ribosomes on the same mRNA as string on beads). Best case scenario you can see things like Nissl granules in neurons which are made up of large amounts of rough endplasmic reticulum that stains well with basic dyes because o...

75RR wrote: ↑

Tue Nov 03, 2020 6:37 pm

... now I'm considering a new Olympus microscope.

New to you or new new?

Any rough clue how big of a hole a new new Olympus with DIC and planapos would burn in my pocket?

Beautiful specimen and great shot 75'

Microworld Steve wrote: ↑

Mon Oct 26, 2020 6:04 pm

Awesome pictures.

Thank you Steve!

Thank you 75' and Jochen Where did you get the samples Wes? These are from a strew slide I bought from Diatom Lab about a year ago. I don't know how he does it but there are virtually no silicates or other junk in the slide, its only diatoms and spicules. At some point I tried making strews myself b...

Chris Dee wrote: ↑

Sun Oct 25, 2020 5:56 am

Like how you've highlighted the geometry of the second one Wes, nice work as always.

Thanks Chris, I decided against stacking the second one through its entire depth exactly as you said to emphasize the geometry.