Search found 3682 matches

by mrsonchus
Sat Nov 02, 2019 9:35 pm
Forum: Specimens, samples and slides
Topic: DufayColor, réseau layer
Replies: 8
Views: 2691

Re: DufayColor, réseau layer

Looked it up Michael, my interest was pricked also! What a remarkable idea this was.
Judging by some of the images from searches, this worked really very well!
Quite amazing. Thanks for posting Michael, an intriguing post.
by mrsonchus
Thu Oct 31, 2019 10:22 am
Forum: Pictures and Videos
Topic: Podocystis adriatica and Cocconeis sp
Replies: 9
Views: 1296

Re: Podocystis adriatica and Cocconeis sp

Lovely image 75'.

What have you done to me?! :shock:

I just visited algaebase to look this one up!

Amazing images, easily detailed-enough for taxonomic use surely.

Thanks again 75'. :D
by mrsonchus
Wed Oct 30, 2019 9:38 am
Forum: Pictures and Videos
Topic: Lacrymaria, pluerostomatid and pretty ciliates
Replies: 10
Views: 2974

Re: Lacrymaria, pluerostomatid and pretty ciliates

Not my area, but had a couple of watches of theses videos. The second video is simply superb and as others have said, your technique is very good indeed and makes for a very interesting video. The 3D nature and internal details are beautifully caught and I also like the other 'bit' that upon 'coming...
by mrsonchus
Wed Oct 30, 2019 9:30 am
Forum: My microscope
Topic: A new microscope. Go big or go home.
Replies: 35
Views: 7862

Re: A new microscope. Go big or go home.

Ohhhhhh..

That, as has been said, is one gargantuan beauty of a 'scope!
I can almost smell it's quality here in the U.K..

Love to see and know more as you use it.
by mrsonchus
Sun Oct 27, 2019 11:19 pm
Forum: Pictures and Videos
Topic: Sequoia gigantensis cross section in Epi-Fluorescence
Replies: 18
Views: 6460

Re: Sequoia gigantensis cross section in Epi-Fluorescence

Hi, the resin-based mountant I use is 'Omnimount' at this time. Wacker's own blocks - great! National Diagnostics is an Omnimount supplier Here's the link to it's page... I'm probably going to switch over to Histomount sometime soon however. I still prefer Histoclear 1 to Histoclear 2, so don't real...
by mrsonchus
Sun Oct 27, 2019 8:48 pm
Forum: Pictures and Videos
Topic: Sequoia gigantensis cross section in Epi-Fluorescence
Replies: 18
Views: 6460

Re: Sequoia gigantensis cross section in Epi-Fluorescence

Hi, very interesting indeed - I just sectioned a dozen Lily-leaf TS slides as I'm experimenting with what used to be called 'reimbibition' would you believe! Basically placing a roughed wax block (roughed to expose tissue cut-face) into water or similar as a way of mitigating the toughness of some t...
by mrsonchus
Sun Oct 27, 2019 12:48 pm
Forum: Pictures and Videos
Topic: Sequoia gigantensis cross section in Epi-Fluorescence
Replies: 18
Views: 6460

Re: Sequoia gigantensis cross section in Epi-Fluorescence

Excellent! The layers 'S1,S2 and S3' of the fiber secondary cell-walls are easily distinguishable. There's a whole-lot more to this technique also, I would say at a first look. There are degrees of subtlety to texture and tone that add to the information content to my eye. It woulld be interesting t...
by mrsonchus
Sun Oct 27, 2019 8:11 am
Forum: Pictures and Videos
Topic: Sequoia gigantensis cross section in Epi-Fluorescence
Replies: 18
Views: 6460

Re: Sequoia gigantensis cross section in Epi-Fluorescence

Must agree!
Startlingly-good images!

Have you any at a higher-mag? Great to peer-into them a little closer for some of the superb details.
Excellent post.
by mrsonchus
Sat Oct 26, 2019 5:13 am
Forum: Pictures and Videos
Topic: Closterium
Replies: 3
Views: 1837

Re: Closterium

Very nice, clear image.
Nicely-done!
by mrsonchus
Sun Oct 20, 2019 10:35 pm
Forum: Pictures and Videos
Topic: Marine Alga
Replies: 7
Views: 1617

Re: Marine Alga

75', this may be a stupid question, but what is needed for a start with DIC, re my BX40?
by mrsonchus
Sun Oct 20, 2019 10:18 pm
Forum: Pictures and Videos
Topic: Marine Alga
Replies: 7
Views: 1617

Re: Marine Alga

What an image 75'!

Such detail, this DIC is pretty impressive! Very nicely-done old chap.
by mrsonchus
Sat Oct 19, 2019 11:18 pm
Forum: Microscopes and optics
Topic: Determining the molecular organisation of biological materials using polarized light microscopy
Replies: 6
Views: 1791

Re: Determining the molecular organisation of biological materials using polarized light microscopy

Hi Wes, here are a few images of longitudinal sections (permanently-mounted & stained) of Sonchus (AKA 'milkweed' I think) stems, as seen with polarisation and the full wave plate. Each image is at a different orientation and shows as in your excellent images, fiber orientation variance relative to ...
by mrsonchus
Sat Oct 19, 2019 8:16 pm
Forum: For forum members who want to buy and sell equipment
Topic: NO LONGER WANTED - Olympus nosepiece adapter
Replies: 9
Views: 3073

Re: NO LONGER WANTED - Olympus nosepiece adapter

75RR wrote:
Sat Oct 19, 2019 7:37 pm
Excellent news! Congratulations.
Thanks 75' - I'm so pleased I'm almost purring this evening!

You look like a fellow that needs a BX40 to me my friend! :D :D ;)
by mrsonchus
Sat Oct 19, 2019 8:15 pm
Forum: For forum members who want to buy and sell equipment
Topic: NO LONGER WANTED - Olympus nosepiece adapter
Replies: 9
Views: 3073

Re: NO LONGER WANTED - Olympus nosepiece adapter

EXACTLY my friend - that's the part I have been looking for to enable the major upgrade of my BX40. I found another BX40 with one fitted, and bought it. Now I'm selling it on and my BX40 has a lovely 6-way removable nosepiece thanks to the adapter! The part name is 'READB' - not easy to find, not ea...
by mrsonchus
Sat Oct 19, 2019 7:52 pm
Forum: For forum members who want to buy and sell equipment
Topic: SOLD SOLD SOLD - OLYMPUS BX40 COMPLETE WITH UIS2 OBJECTIVES
Replies: 0
Views: 2106

SOLD SOLD SOLD - OLYMPUS BX40 COMPLETE WITH UIS2 OBJECTIVES

Hi all, I have a spare Olympus BX40 that I'm offering for sale.

This one is available as I bought 2 to effectively make one, that I now use, and have this one to sell-on.

Now sold all, thanks for looking!
by mrsonchus
Sat Oct 19, 2019 7:26 pm
Forum: For forum members who want to buy and sell equipment
Topic: NO LONGER WANTED - Olympus nosepiece adapter
Replies: 9
Views: 3073

Re: NO LONGER WANTED - Olympus nosepiece adapter

75! Well, erm, the thing-is my friend, I saw then bought another BX40, for a very affordable price that will allow me to sell it on at a sensible price... The 'extra' BX40 arrived safely and has all the parts I'm after - and more! For starters, it had, past-tense :D , the adapter to enable the use o...
by mrsonchus
Sat Oct 19, 2019 11:16 am
Forum: For forum members who want to buy and sell equipment
Topic: NO LONGER WANTED - Olympus nosepiece adapter
Replies: 9
Views: 3073

Re: NO LONGER WANTED - Olympus nosepiece adapter

Hi Bob, yes there's a tiny 'piece of dovetail-profile' as it were, that the set-screw pushed against. This then, being a nice fit for the edge of the adapter, by virtue of it's matching profile, completes the fit. This image shows the tiny piece in-situ, https://lh3.googleusercontent.com/0f71RZ7EENB...
by mrsonchus
Sat Oct 19, 2019 9:01 am
Forum: Microscopes and optics
Topic: Determining the molecular organisation of biological materials using polarized light microscopy
Replies: 6
Views: 1791

Re: Determining the molecular organisation of biological materials using polarized light microscopy

Lovely and as said informative post Wes! These spirals are in fact the lignified secondary cell-walls from the Xylem-vessels of a plant. The primary cell-wall (which would have covered the spirals like a sleeve as it were) are gone and just the reinforcing spirals of lignin remain. The reason they'r...
by mrsonchus
Thu Oct 17, 2019 10:46 pm
Forum: For forum members who want to buy and sell equipment
Topic: NO LONGER WANTED - Olympus nosepiece adapter
Replies: 9
Views: 3073

NO LONGER WANTED - Olympus nosepiece adapter

Hi all,

this request is now over - I have found the parts I need!
by mrsonchus
Thu Oct 17, 2019 10:45 pm
Forum: For forum members who want to buy and sell equipment
Topic: NO LONGER WANTED Olympus intermediate polariser
Replies: 31
Views: 6080

NO LONGER NEEDED Olympus intermediate polariser

Hi all,

this request is now over - I have found the polarising parts I need!
by mrsonchus
Tue Oct 15, 2019 11:07 pm
Forum: Microscopes and optics
Topic: Mixed FN objectives on same head?
Replies: 7
Views: 1709

Re: Mixed FN objectives on same head?

Hi Pete, I see what you mean.. I also have (it arrived today) an aplanat/achromat 1.4 n.a. condenser that doesn't show this effect with either of the 60x objectives of n.a. 0.8 and 0.9. What I have subsequently found after some testing and comparisons this afternoon is that the apparent resolution a...
by mrsonchus
Tue Oct 15, 2019 8:37 am
Forum: Microscopes and optics
Topic: Mixed FN objectives on same head?
Replies: 7
Views: 1709

Re: Mixed FN objectives on same head?

Hi Michael, I don't know but the super and norma widefield eyepieces look the same size.. What would be the reasoning for this? It's an interesting question though. one I may contact Olympus about? The cheapo Chinese 60x 0.8 n.a. FN22 objective I have has exctly the same image in terms of brightness...
by mrsonchus
Tue Oct 15, 2019 6:20 am
Forum: Microscopes and optics
Topic: Mixed FN objectives on same head?
Replies: 7
Views: 1709

Re: Mixed FN objectives on same head?

Thanks Pete. There's a difference though when setting the condenser's n.a. to that of the objective. When the rear focal plane of the objective is viewed through a phase telescope the condenser's iris is correctly the width of the view (i.e. the condenser's n.a. matches that of the objective at 0.9)...
by mrsonchus
Mon Oct 14, 2019 10:53 pm
Forum: Microscopes and optics
Topic: Mixed FN objectives on same head?
Replies: 7
Views: 1709

Mixed FN objectives on same head?

Hi all,a question that intrigues me. Microscope heads for the olympus BX infinity range come in a 'normal widefield' version, with 22mm FN eyepieces and 22mm FN objectives below. There is also a 'super widefiled' head with 26.5 FN eyepieces (SWH10x-H for example) and 26.5mm FN objectives below. Ques...
by mrsonchus
Mon Oct 14, 2019 4:01 pm
Forum: Pictures and Videos
Topic: Pollen. There's lots of it about.
Replies: 100
Views: 67295

Re: Pollen. There's lots of it about.

What a beautiful little plant! I like the hidden pollen-grains too.
Nicely-done.
by mrsonchus
Sat Oct 12, 2019 10:13 pm
Forum: My microscope
Topic: DIY DIC Effect on Darkfield.... unreal
Replies: 6
Views: 2766

Re: DIY DIC Effect on Darkfield.... unreal

Hmm, there's a touch of oblique there for sure, but DIC - I don't think so. Pretty nice to experiment though, keep at it as these little surprises are by no means rare with microscopy.... By the way, your microscope looks to be a nice instrument also. Keep exploring, experimenting and sharing - you'...
by mrsonchus
Sat Oct 12, 2019 8:42 am
Forum: Beginner's corner
Topic: Fusing glass slides
Replies: 23
Views: 4836

Re: Fusing glass slides

I don't suppose some-sort of glass cuvette, perhaps even 'sawn-off', would be any use?
by mrsonchus
Sat Oct 12, 2019 8:34 am
Forum: Beginner's corner
Topic: Newbie with information overload looking for first microscope
Replies: 22
Views: 4169

Re: Newbie with information overload looking for first microscope

What a lovely-looking 'scope that is!
I must agree with your choice of teaching-head, perfect for the kids.

You're all in for a real treat, great to see microscopy being explored together, congratulations! :D :D
by mrsonchus
Thu Oct 10, 2019 10:54 pm
Forum: Camera systems and imaging
Topic: Trinocular Modification for an Olympus CX31?
Replies: 17
Views: 3826

Re: Trinocular Modification for an Olympus CX31?

I agree completely Gene, it's always just a matter of time I find.
I like the look of the stereo-zoom trinoc, but way-above my budget I'm afraid old chap. If I hear of any interest I'll surely let you know.
by mrsonchus
Thu Oct 10, 2019 8:19 pm
Forum: Camera systems and imaging
Topic: Trinocular Modification for an Olympus CX31?
Replies: 17
Views: 3826

Re: Trinocular Modification for an Olympus CX31?

That sounds about right Gene. The choice of the side-port intermediate or the trinocular.

Incidentally, did you find a supplier for the U-TRU, as I'm looking for one too?