MicrobeHunter.com Microscopy Forum

Hi Niklas, I can try! Can you give me a better idea of what I need to be doing?

Hi, The BH-2-RLA-2 fitting also has an iris (field iris allowing the elimination of halos with the DF settings and N.A. matching presumably) with a BD drop-in filter I bought recently to go with it in the sale. There's an ND-filter too, that automatically flips in (and may be flipped-out again if pr...

Hi 75' - quite a coincidence! When I saw this thread a couple of days ago I mentioned it to my Wife who said I should sell it on and stop moaning about space! So, finally I'm getting a listing built. But it may be O.K. for Niklas, it's in lovely working condition. Oh yes, here's a link to a set of i...

Hi, I may have a 'scope that may suit you. It's an Olympus BHM with infinity objectives, Neo-Splan LWD-50x and Neo-Splan 20x, DPlans at 10x and 5x. This 'scope is able to give incident and darkfield incident as it has the BH2 RL attachment. I'm just about to put it onto e-bay (U.K.) and it will prob...

Scarodactyl wrote: ↑

Mon Aug 24, 2020 1:09 am

What's your budget range? There are some good used CMO options but it depends on how much you want to shell out.

pm sent

Sent a pm Apo'.

Hi all, I'm really quite fed-up with the tragic images coming through the trinocular photo-port of my generic (badged as Amscope) trinocular zoom stereo 'scope. It's of the type where the left eyepiece is given-over to the photo light-path with the pull of a pell/push bar. Also it's the usual Greeno...

Many thanks all, very kind comments.

Thanks John, those threads were great! Fantastic detail! It's nice to see the kind of results you can get with hand-sectioning. Would you say those stains are a better bet to start off with than Eyosin and Hematoxalin? Cheers Markus Without a shadow of doubt yes - Haematoxylin (I use Harris for nuc...

They certainly do add to hand sections, see here
See here too....

Suggest, safranin (powder to mix yourself), alcian-blue - both aqueous, both cheap and available as powders!

Cheers! The first one (the elongated) looks very much like Navicula digitoradiata Longueur : 88 microm. Largeur : 13,8microm. 10-11 stries / 10 microm. (from a collection of Mediterranean diatom photos). Looks very, very like it Hobby' - here's a link with a good likeness... It also seems to have a...

Here's another one - trying-out ToupView's measuring features again.

Hobbyst46 wrote: ↑

Fri Aug 21, 2020 3:07 pm

Very nice images !
The distance of 2.98 um indicates a very high density of stria (~39/10um).

I think you're right! It seems I may well have cropped this one during PP - see revised measurement of 85µ....

Aha! I thought the measured diatom was a little small.... I think I must have cropped the image while PP. Here's what I think is the uncropped and correctly-measured version - 4x the size at 80+µ (oops :oops: ) https://lh3.googleusercontent.com/pw/ACtC-3c5NHBEVnA4MFg0T4AvrrWCSGnZJLMLVKQV-lOgsJR2_YGr...

MicroBob wrote: ↑

Fri Aug 21, 2020 6:09 pm

Hi John,
with a tungsten carbide knife it might be possible to section them and very little work has been done on staining them!

Bob

Help-me!
Bob, you are bonkers!

75RR wrote: ↑

Fri Aug 21, 2020 4:03 pm

Picking up some very nice detail - the dark side will get you yet!

ARRRGGHHHHH! Never - leaves for ever! (although these Diatom-thingies are quite nice...)

Thanks both for your comments.

Hi all, a few images to share. As most know, my area is Botanical, but I've been rummaging through some rarely-opened drawers and boxes in my lab with a view to tidying-up (or at least trying to...). Anyway I found an old slide with Diatoms on it that I must have acquired with some of the old mixtur...

Hi shawn. If you mean the wax-blocks (containing specimen for sectioning), I use those cheap plastic divided boxes that sets of screws etc come in. As far as ambient environment, room-temp is fine. The blocks really do keep well for several years at least, in my experience. I often have 'left over' ...

Hi, can you give a little more detail please. I use TV quite a lot and may be able to help...

Hi, well I haven't used this combination myself before although I've used and have both of them. My use is entirely for Botanical sectioned and mounted, sometimes 'live', tissue. What these 2 are though is, on paper a reasonable pairing. The MB is as you say a nuclear stain that also may be used as ...

Same here, I definitely prefer eyecups during observation but am unable, as a wearer of glasses, to use them. The distance from the eyepiece glass and the amount of light intruding around my glasses then makes it impractical. The difference in the viewing 'experience' is to me anyway so much better ...

Ha! Fell into my fiendish trap!

That's not a 'tilt' - it's merely that the head isn't quite turned to the right position to line up. Just turn the head very slightly to make the line horizontal as it were - no problem there! It'll be a tiny amount either CW or ACW of course.

I think I have one of those in my drawer - I have a look at it after tea.

The one I use is even more recent (this June), see this post.

Hmm, an interesting comparison but the setup isn't quite good enough to make it valid. The slides aren't so bad, the woody one has some interesting features in cluding 'expanded ray' tissue - a product of fast post-1st year growth where the rays have to grow 'sideways' to keep up with the rapid incr...

Hi, this sometimes happens with TView on my Linx tabled (32-bit) but never on my Laptops (64-bit), all Windows 10. I suspect that it's either a 32 vs 64-bit difference (in either the TV-software version or the op-sys version) or a resource shortage in the 'smaller' Linx - which has less of everythin...

Ah.. too small drat!

Hi all, I don't suppose this adapter would do the trick for a 60D? I use a similar one for my 200D. A really handy item. A spare cover is cheap enough too - so a hole may be made for the cable. The 200D (as-did my previous 1200D) has a slot fitted, with a little rubber plug. Here's a link to a spare...

? The Olympus dovetail I refer to is the one at the bottom of the U-SPT - I use on with a (for infinity in my case) PE2.5x in the top. The item to which I linked has no dovetail, just a straight connector that looks like it will go into your photoport directly - leaving the c-mount available... The ...