Search found 3682 matches

by mrsonchus
Wed Apr 22, 2020 5:12 pm
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3183

Re: Pollen Fine Detail

Looks like a sterling-start to me Bob! There's a little detail peeking out of the spines I can see. You may find stacking to be sub-optimal with these subjects - the tendency is great to 'overstack' beyond what is effectively a 'top surface' - albeit a 3D one. If this happens, as is very common with...
by mrsonchus
Wed Apr 22, 2020 5:06 pm
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3183

Re: Pollen Fine Detail

Looks like a sterling-start to me Bob! There's a little detail peeking out of the spines I can see. You may find stacking to be sub-optimal with these subjects - the tendency is great to 'overstack' beyond what is effectively a 'top surface' - albeit a 3D one. If this happens, as is very common with...
by mrsonchus
Wed Apr 22, 2020 10:13 am
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3183

Re: Pollen Fine Detail

Hi John I will definitely have to try that. I have been looking at all these beautiful dandelions over the last few days. Go get-em Roldorf. The tap root of the Dandelion is very easy to hand section - dig-up the whole root (usually about 6 inches deep) for some very nice images - stain with just a...
by mrsonchus
Wed Apr 22, 2020 9:57 am
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3183

Re: Pollen Fine Detail

Good morning all - a lovely sunny morning here in the U.K. - wildflowers sprouting-up everywhere in our gardens, and all-day to study them! :D Many thanks for all your kind comments, it's always great when others share an adventure. MicroBob, The fuchsine (probably the de-facto stain for pollen, at ...
by mrsonchus
Tue Apr 21, 2020 11:53 pm
Forum: Pictures and Videos
Topic: Pollen Fine Detail
Replies: 47
Views: 3183

Pollen Fine Detail

Hi all, I've been mounting pollen lately, fresh from the plant into fuchsin-infused glycerin-jelly, with coverslip. These make pretty good long-term but temporary mounts and are very simple to prepare. Simply put, place a slide and coverslip (seperate from each other not one on top of the other) ont...
by mrsonchus
Tue Apr 21, 2020 9:50 am
Forum: For forum members who want to buy and sell equipment
Topic: Found: Leitz K4 condenser lens (Can a mod please move this to the equipment discussion? Thanks!)
Replies: 34
Views: 1536

Re: WTB: Leitz K4 condenser lens

Opps, I'll see if I can import the images into the forum's host, back soon! :oops:

Should be OK now folks.
by mrsonchus
Mon Apr 20, 2020 11:29 pm
Forum: For forum members who want to buy and sell equipment
Topic: Found: Leitz K4 condenser lens (Can a mod please move this to the equipment discussion? Thanks!)
Replies: 34
Views: 1536

Re: WTB: Leitz K4 condenser lens

What is it :o ? No such a lens came with my Orthoplan. It is not mentioned in the Orthoplan manual or other materia I have. Here's a snip from Leitz info, K1 vs K4 inserts.jpg This is the one I had in my Orthoplan's condenser.... ws_condenser K4 arrival and fitting (18).jpg They're not as expensive...
by mrsonchus
Fri Apr 10, 2020 7:31 pm
Forum: Microscopes and optics
Topic: oil condenser questions
Replies: 5
Views: 606

Re: oil condenser questions

Leitz did also produce a 1.4 (oil) condenser for the Orthoplan - I had one with my Orthoplan.
by mrsonchus
Mon Apr 06, 2020 11:16 am
Forum: Resources (online, books etc.)
Topic: Lighting
Replies: 3
Views: 343

Re: Lighting

An excellent video, many thanks. This video has been very useful to me, although I know how and why to use Koehler illumination after quite a time with my microscope. BUT, what this video did 'illuminate' for me was the events that occur with a past microscope of mine, namely the Leitz Orthoplan, wh...
by mrsonchus
Fri Apr 03, 2020 11:15 pm
Forum: For forum members who want to buy and sell equipment
Topic: Stereomicroscope Help
Replies: 10
Views: 594

Re: Stereomicroscope Help

Just to echo the above re Brunel Microscopes. I've purchase a brand new SP200, a stereo 'scope and a used Leitz Orthoplan together with a lot of lab supplies, over the last 5-6 years. They're superb, all of the above is also my experience with them. Good honest folk In my experience.
by mrsonchus
Fri Apr 03, 2020 3:30 pm
Forum: For forum members who want to buy and sell equipment
Topic: Microscope parts for sale and identification
Replies: 13
Views: 2765

Re: Microscope parts for sale and identification

Hi, coincidentally I came across the box with these in yesterday as I was having a clear-up in my lab. I'm pretty sure I still have them, any particular item/s you're interested in old chap?
by mrsonchus
Fri Apr 03, 2020 1:26 am
Forum: Specimens, samples and slides
Topic: Apple Twig Hand Section, Stain & Mount
Replies: 9
Views: 468

Apple Twig Hand Section, Stain & Mount

Hi all, I've been having a clean and tidy in my 'lab' and whilst doing so had a quick go at some crude hand sectioning of some of the small twigs on one of our dwarf apple trees, which are just starting to elongate their buds. Only really did this as I was distracted by said tree while taking the do...
by mrsonchus
Sat Mar 28, 2020 10:48 am
Forum: Beginner's corner
Topic: Amscope T720 vs. Swift SW380T
Replies: 12
Views: 1092

Re: Amscope T720 vs. Swift SW380T

Hi, this forum is packed with very knowledgeable folk re equipment. Yours is an often-asked question of course, and a look through past posts - many very recent or active, will get you off to an interesting and useful start. My one piece of advice is to buy nothing until you've spent a little time w...
by mrsonchus
Tue Mar 24, 2020 9:21 am
Forum: Miscellaneous
Topic: Large SEM of Paralia sulcata
Replies: 7
Views: 847

Re: Large SEM of Paralia sulcata

Urk - those SEM images are quite stunning - the detail & complexity is extraordinary. BUT, all you diatom-botherers must not let this discourage you, rather let it enliven, augment and increase your enjoyment - every session of observation has the chance of revealing that known but as-yet unseen ext...
by mrsonchus
Fri Mar 20, 2020 9:30 pm
Forum: Pictures and Videos
Topic: Close-up of a stentor sp.
Replies: 8
Views: 1418

Re: Close-up of a stentor sp.

Nice video - very crisp and some good detail.
by mrsonchus
Sun Mar 15, 2020 12:50 am
Forum: Beginner's corner
Topic: What is this and what is it for?
Replies: 2
Views: 1811

Re: What is this and what is it for?

Aha! It's a rudimentary hand-section cutter - for soft stems of small diameter. Push a small soft stem piece through one of the holes, from below. Set it to just be sticking up above the hole's side by about 1mm then turn the blade-holding part to cut a section. Place under your 'scope - in a drop o...
by mrsonchus
Wed Mar 04, 2020 4:49 pm
Forum: Specimens, samples and slides
Topic: Moss leaves in glycerin: how to prevent cells from collapsing?
Replies: 9
Views: 7192

Re: Moss leaves in glycerin: how to prevent cells from collapsing?

You may try a weak glycerin/water mix - perhaps 1:10 (v/v) - but with a glycerin content that is enough to cover the sample if used on it's own. The idea being to put the leaves into the mixture then allow the water content to slowly evaporate - thus gradually increasing the concentration of glyceri...
by mrsonchus
Wed Mar 04, 2020 12:13 pm
Forum: Identification help
Topic: Entomoneis
Replies: 10
Views: 3476

Re: Entomoneis

Urk - I see what you mean - can't open it. AVS doc converter says it's an invalid or broken file also.... I'll have a closer look at it and get back.
by mrsonchus
Wed Mar 04, 2020 12:07 pm
Forum: Identification help
Topic: Entomoneis
Replies: 10
Views: 3476

Re: Entomoneis

Once downloaded it'll open with Chrome?
by mrsonchus
Wed Mar 04, 2020 11:52 am
Forum: Identification help
Topic: Entomoneis
Replies: 10
Views: 3476

Re: Entomoneis

Hi 75' Here's a link to the pdf in my shared g-drive... When you click on the link the message will tell you that the document can't be viewed - but it CAN be downloaded - look top RHS for the download icon on this dark message screen - it'll download to your machine for you - I hope... See this scr...
by mrsonchus
Sat Feb 29, 2020 6:55 pm
Forum: Pictures and Videos
Topic: More living diatoms II
Replies: 7
Views: 1778

Re: More living diatoms II

A really nice set of images!
by mrsonchus
Thu Feb 27, 2020 6:57 pm
Forum: Beginner's corner
Topic: Disadvantages to Phase Contrast objectives?
Replies: 19
Views: 5792

Re: Disadvantages to Phase Contrast objectives?

Hi Apo' and Hobby' - I'd agree - definite differences - perhaps made a tad murky by jpg, but they exist. Good enough though for the purchase of a phase objective whilst waiting for a non-phase (assuming the use of phase is also relevant of course). What we need is some better images for comparison -...
by mrsonchus
Thu Feb 27, 2020 2:41 pm
Forum: Beginner's corner
Topic: Disadvantages to Phase Contrast objectives?
Replies: 19
Views: 5792

Re: Disadvantages to Phase Contrast objectives?

ARRGGHHHH!!! Goomba - I just accidentally 'reported' your post - oops - I meant to use the quote button but accidentally used the exclamation-mark (report-post button...) - apologies :oops: :oops: I'll PP these images also to see just how close they can get to each other. The posted 'faithful' versi...
by mrsonchus
Thu Feb 27, 2020 1:31 pm
Forum: Beginner's corner
Topic: Disadvantages to Phase Contrast objectives?
Replies: 19
Views: 5792

Re: Disadvantages to Phase Contrast objectives?

Hi, I just had a quick look at a couple of images for a rough comparison. Comparing an Achromat Ph2 40x Olympus infinity objective to an Olympus PlanC N UIS2 'ordinary' BF objective, as image, WS_phase nosepiece (3).jpg I also keep a phase and BF nosepiece, WS_phase nosepiece (2).jpg The subject isn...
by mrsonchus
Sun Feb 23, 2020 3:05 pm
Forum: Beginner's corner
Topic: starting with olympus CX23
Replies: 3
Views: 2049

Re: starting with olympus CX23

Hi, I'd say you have a cracking start with the Olympus!
by mrsonchus
Thu Feb 20, 2020 8:47 pm
Forum: Camera systems and imaging
Topic: Video stitching and layering
Replies: 7
Views: 2127

Re: Video stitching and layering

Hi, I posted a video on Youtube of the process myself some time ago now -
by mrsonchus
Thu Feb 20, 2020 7:28 am
Forum: Camera systems and imaging
Topic: Video stitching and layering
Replies: 7
Views: 2127

Re: Video stitching and layering

Microsoft ICE is superb and fast for stitching.....
by mrsonchus
Sat Feb 15, 2020 10:17 pm
Forum: Introduce yourself
Topic: Hello from Tehran :)
Replies: 13
Views: 4800

Re: Hello from Tehran :)

That's why it's anatomy isn't the unifacial stem type - it's a petiole ('leaf stalk') - a little like a stem that hasn't fully 'wrapped into a cylinder' as it were - hence the lack of a ring of vascular bundles etc. In fact Bob's also looks like a petiole rather than a stem section - a lovely fresh ...
by mrsonchus
Sat Feb 15, 2020 3:57 pm
Forum: Microscopes and optics
Topic: isn't this a good deal for a Leica microscope ?
Replies: 31
Views: 11997

Re: isn't this a good deal for a Leica microscope ?

Hi Marco, a nice looking 'scope! Perhaps a variable neutral -density filter (as screwed onto the fron of a camera) on top of the light port will help. With this you'll be able to to keep the halogen at it's whitest full-ish setting and control light intensity with a turn of the rotating VND filter.....
by mrsonchus
Sun Feb 09, 2020 9:09 pm
Forum: Pictures and Videos
Topic: Nitzschia sigmoidea
Replies: 10
Views: 3334

Re: Nitzschia sigmoidea

I think you're right 75' - I definitely see different effects with the various (Weighted-Average, Depth-Map, Pyramid) options in Helicon Focus - especially I suspect with the very often 'soft' images of obscure detail within a mounted-section rather than optimally at the coverslip's underside.