Search found 3682 matches

by mrsonchus
Sat Feb 14, 2015 3:06 am
Forum: Microscopes and optics
Topic: Incident lighting on a budget
Replies: 6
Views: 1959

Incident lighting on a budget

Hi all, had a go at setting up incident-lighting to look at some larger specimens of seeds (placed in a well-slide). I've a little bottom-lighting too which gave a nice background although I think I'd have been better off with an opaque black or white card under the seeds... I got the bendy USB LED ...
by mrsonchus
Fri Feb 13, 2015 11:31 pm
Forum: Beginner's corner
Topic: making staining jelly
Replies: 4
Views: 2344

Re: making staining jelly

Hi, add some Fuschin until it's a strong vivid red (this will take less that you think) to the 96% alcohol. Try perhaps 5ml of the alcohol to a tiny pinch of powder (on end of teaspoon handle maybe) and keep adding until its about as transparent as very rich strawberry jam.... Let it mix and settle ...
by mrsonchus
Fri Feb 13, 2015 11:01 pm
Forum: Pictures and Videos
Topic: Pollen slides revisited
Replies: 4
Views: 1164

Re: Pollen slides revisited

I would like a step by step recipe for make small amounts of glycerin jelly with basic Fuchsin added. I have what I need to make it I just am not sure how to dissolve the basic Fuchsin and I do not want a huge amount. Hmm - I'm not absolutely sure, I bought mine ready-made complete with Fuchsin in ...
by mrsonchus
Fri Feb 13, 2015 4:03 am
Forum: Pictures and Videos
Topic: Quelques diatomées
Replies: 7
Views: 1490

Re: Quelques diatomées

Superb - especially the first & last!
by mrsonchus
Fri Feb 13, 2015 3:59 am
Forum: Pictures and Videos
Topic: Diatomée
Replies: 5
Views: 1252

Re: Diatomée

Nice coups de créatures fascinantes. Excuses pour l'horrible Français.
by mrsonchus
Fri Feb 13, 2015 3:47 am
Forum: Pictures and Videos
Topic: First Rheinberg attempt
Replies: 5
Views: 1121

Re: First Rheinberg attempt

Great!!! I like trying different Rheinberg filters. You can get totally different looks and if you do one you don't like, you can throw it away and move on to the nexr one. Very good image. Good DOF throughout the FOV. Thanks JimT. I think I'll definitely have a try at some more techniques involvin...
by mrsonchus
Fri Feb 13, 2015 3:41 am
Forum: Pictures and Videos
Topic: First Rheinberg attempt
Replies: 5
Views: 1121

Re: First Rheinberg attempt

vasselle wrote:Bonjour
Très beau avec de très belle couleur
Cordialement seb
Merci beaucoup Vasselle.
by mrsonchus
Thu Feb 12, 2015 11:27 pm
Forum: Pictures and Videos
Topic: First Rheinberg attempt
Replies: 5
Views: 1121

First Rheinberg attempt

Hi all, I've finally had a chance to try Rheinberg illumination. My new 'scope had no filter tray beneath the condenser - only a swing-out bullyseye lens to fill the FOV of the 4x objective with light completely. The ever helpful Brunel sent me another condenser free of charge , that does have said ...
by mrsonchus
Thu Feb 12, 2015 9:06 pm
Forum: Pictures and Videos
Topic: Pine Cone Seed Longitudinal Section
Replies: 7
Views: 1371

Re: Pine Cone Seed Longitudinal Section

Thanks very much John. Everything I see these days causes me to wonder how it would look under the scope. I picked up the microtome on Ebay. It works well if the driver does his job. Here is a shot of it. Looks a beauty - I'd love to try one of those. I think the preferred blade to use may often be...
by mrsonchus
Thu Feb 12, 2015 8:50 pm
Forum: Introduce yourself
Topic: Hello from Maine
Replies: 9
Views: 2971

Re: Hello from Maine

Welcome back! I'm gradually learning how to collect, process and permanently mount pollen too! I've the very start of a collection - a couple of varieties of Sow Thistles (Sonchus) that are a pesky weed in the UK. I've learned an awful lot from WD's little book, all his material including everything...
by mrsonchus
Thu Feb 12, 2015 1:19 pm
Forum: Pictures and Videos
Topic: My first feeble attempts to stain & mount onion epidermis...
Replies: 5
Views: 1344

Re: My first feeble attempts to stain & mount onion epidermi

Crater Eddie wrote:Looks good from here! Makes me want to give it a go.
CE
Thank you, Walter Dioni inspired me to try the onion epidermis, have a look at the website and his
contributions - you'll soon get bitten by the bug!

regards
by mrsonchus
Thu Feb 12, 2015 3:57 am
Forum: Pictures and Videos
Topic: A couple more human blood photos.
Replies: 9
Views: 1696

Re: A couple more human blood photos.

Thank you! And, as expected, the clear nail polish is not suitable, as it caused much of the stain to migrate to the edges of the cover slip. It was quite interesting to watch. Also, the glycerin is having a similar effect, though not as pronounced and in much slower motion. CE These are really goo...
by mrsonchus
Thu Feb 12, 2015 3:48 am
Forum: Pictures and Videos
Topic: Pine Cone Seed Longitudinal Section
Replies: 7
Views: 1371

Re: Pine Cone Seed Longitudinal Section

Love these pictures - good old Pine eh! I really like your exploratory approach - these are colourful and interesting pictures and I think you did well to get them. What microtome did you use?

John
by mrsonchus
Thu Feb 12, 2015 3:28 am
Forum: Pictures and Videos
Topic: Pollen slides revisited
Replies: 4
Views: 1164

Re: Pollen slides revisited

Very nice indeed! If I may critique the 2nd image, I would have not cleaned the background "wholesale", but rather cloned out really objectionable, undefined objects individually and left the other stuff alone: I think that might have given it a more "natural" look. All the same, very nice images, ...
by mrsonchus
Thu Feb 12, 2015 1:29 am
Forum: Pictures and Videos
Topic: Cross section of aneedle of Picea spec.
Replies: 10
Views: 1882

Re: Cross section of aneedle of Picea spec.

Manfred wrote:Rod,

it is also named "Etzold FCA" and is a mixture of Fuchsin, Chrysoidin and Alcianblau (sorry but I don`t know in English).
It is usable for all plant sections and gives nice results.

Manfred
This stain certainly does a good job - I wonder what the 'UK version' is sold as?
by mrsonchus
Thu Feb 12, 2015 1:26 am
Forum: Microscopes and optics
Topic: First scope advice
Replies: 27
Views: 5558

Re: First scope advice

mrsonchus, you already have a nice camera for photography unless you want to become a serious photography hobbyist. A 5MP USB camera will be very good for microscope images unless you want to enlarge them to "mega size". Connectable to your lap top, can take TIFF images, and suitable for post proce...
by mrsonchus
Thu Feb 12, 2015 1:10 am
Forum: Pictures and Videos
Topic: Pollen slides revisited
Replies: 4
Views: 1164

Pollen slides revisited

Here are a couple of pictures of some of my first pollen mounts in Glycerin Jelly stained with Fuschin - the backgrounds have been 'everage-blurred' away to clean them up a bit as they are really pretty bitty and mucky. The Schlembergera is better known as 'Christmas Cactus' although the variety fro...
by mrsonchus
Thu Feb 12, 2015 12:47 am
Forum: Pictures and Videos
Topic: My first feeble attempts to stain & mount onion epidermis...
Replies: 5
Views: 1344

My first feeble attempts to stain & mount onion epidermis...

Here are several of my first rather poor attempts to stain onion epidermis with Safranin and Fast Green stains (seperately). The samples were kept for several days in 75% IPA then stained for 2 hours. They were then taken through alcohol/DIW series down to DIW only, then mounted in glycerin and seal...
by mrsonchus
Thu Feb 12, 2015 12:09 am
Forum: Beginner's corner
Topic: Trouble with staining
Replies: 7
Views: 3087

Re: Trouble with staining

Hi JB, looking at the stated ingredients of your stains, these look like they may be to Johansen's formulation, if so, they are intended to be used with differentiating solutions. I do not know either of the books in your links, however, do neither they, nor the suppliers of the stains not to tell ...
by mrsonchus
Wed Feb 11, 2015 11:58 pm
Forum: Pictures and Videos
Topic: Pine Needle II
Replies: 6
Views: 1456

Re: Pine Needle II

Thank you John. I changed the way I held the needles in the microtome and I believe it made for a more secure hold that allowed the knife to do it's job more cleanly. Thanks for responding Rod Manfred's advice is good - perhaps try something bigger for learning technique. I used a nut & bolt but pl...
by mrsonchus
Wed Feb 11, 2015 6:12 am
Forum: Pictures and Videos
Topic: Pine Needle II
Replies: 6
Views: 1456

Re: Pine Needle II

Aha - good section. The clean-cut edges without tearing show that your techniques is pretty good to me. This image has a lot more detail (and information in it) nice result.

John
by mrsonchus
Tue Feb 10, 2015 9:25 pm
Forum: Pictures and Videos
Topic: Cross section of aneedle of Picea spec.
Replies: 10
Views: 1882

Re: Cross section of aneedle of Picea spec.

Manfred wrote:Dear all,
motivated by the section of a pine needle shown by Rod (rnabholz) I tried today the same.
Believe me - its a real challenge.
The section was stained with Wacker FCA.
Wow - superb results, congratulations these are excellent images!
Thanks for the post.

John
by mrsonchus
Tue Feb 10, 2015 9:22 pm
Forum: Pictures and Videos
Topic: Cross section of aneedle of Picea spec.
Replies: 10
Views: 1882

Re: Cross section of aneedle of Picea spec.

Perhaps it is my fresh experience, and less than optimal results speaking, but this is ABSOLUTELY FANTASTIC!! I had planned to take another run at this subject tonight, but your shot may have defined the genre for all time. Anything I might post will look like a mud pie now! ;^) Would love to hear ...
by mrsonchus
Tue Feb 10, 2015 12:23 am
Forum: Pictures and Videos
Topic: Cilantro
Replies: 9
Views: 1651

Re: Cilantro

Very nice images indeed, love the colours and good details - your 'phone takes a better picture than my 2mp dedicated USB camera!

Thanks for the treat and well done.
by mrsonchus
Tue Feb 10, 2015 12:16 am
Forum: Microscopes and optics
Topic: First scope advice
Replies: 27
Views: 5558

Re: First scope advice

My 'scope is crystal-clear with superb resolution, but sadly the 2mp USB camera leaves me disappointed. Which 2MP camera is that and what is the problem with it? Only Celestron Microscope Imager is available here at 84 euros. I could buy a 5MP from Amscope with the same money but I think I try this...
by mrsonchus
Tue Feb 10, 2015 12:02 am
Forum: Microscopes and optics
Topic: First scope advice
Replies: 27
Views: 5558

Re: First scope advice

mrsonchus, I offer the same advice I gave Lymia, a DSLR is only good if you do other photography given the cost Vs. a 5MP USB camera. I have a Canon (my second one) and think they are great! That said, I am not so sure about the 1200D. Probably shouldn't use this site but check out the following: h...
by mrsonchus
Mon Feb 09, 2015 9:54 pm
Forum: Microscopes and optics
Topic: First scope advice
Replies: 27
Views: 5558

Re: First scope advice

Ok, thank you both for your answers. This is exactly what I needed to know. My choice now that I understand: USB, 5 MP. :) Sorry to but-in but I think you're right. I've got a 2mp Toupcam USB 'scope camera attached and whilst it's pretty good as an affordable general-purpose solution it's not reall...
by mrsonchus
Mon Feb 09, 2015 7:38 pm
Forum: Beginner's corner
Topic: Trouble with staining
Replies: 7
Views: 3087

Re: Trouble with staining

Hi JB, What formulations are using when mixing your stain? For what amount of time are you staining, fast green should take between 10 and 20 minutes, safranin can take 1 to 3 days. Peter. Hi Peter, thanks for helping. I'm using the formulations supplied pre-mixed from Brunel and they are in cellos...
by mrsonchus
Mon Feb 09, 2015 7:18 pm
Forum: Beginner's corner
Topic: Trouble with staining
Replies: 7
Views: 3087

Books to learn with

Can't help as I have never done any staining. Just wanted to say that I am impressed by how you have jumped in at the deep end! What book are you using as a guide? I'm learning slowly and to say I'm enjoying it is an understatement! I only have the usual college experience of (gulp) about 35 years ...
by mrsonchus
Mon Feb 09, 2015 12:09 am
Forum: Beginner's corner
Topic: Trouble with staining
Replies: 7
Views: 3087

Trouble with staining

Hi, can anyone give me a bit of advice - I'm practicing with onion epidermis and am having difficulty with staining.... I keep my specimens (the epidermis in this case) in 75% IPA. When moving through a decreasing series of IPA/DI water mixes into aqueous methylene blue and rinses everything goes ve...