MicrobeHunter.com Microscopy Forum

Hello Walter and welcome - I'm new here too and it's a great group of friendly folks!
A very warm welcome and best wishes.

The 60x onion image is good but I see more detail in the 40X. Too much enlargement??? Nice camera. How have you mounted it? I agree, but the 60x is very sensitive to mount quality and the proximity of specimen to underside of coverglass, also the refractive index of the mounting medium and the over...

Nice comparison! I have often thought about getting a 60x objective, and a 20x as well. Darned Ebay sellers aren't cooperating though. :lol: CE Hi, I have a 5-objective 'scope with a 4x, 20x, 40x, 60x (dry) and 100x (oil) - I asked for the 10x to be replaced with the 60x when I bought the 'scope - ...

I believe there some stacking/depth of field applications too somebody recommended here. I'll search for them too. For very quick and easy DOF stacking ' Toupview ' is free, easy to use and can be used with or without a camera input. The latest version is far superior than earlier versions - it mak...

Hi all, I've been having a go at taking multiple pictures down the scope moving across the 'scopes FOV then down, then across etc to cover the whole eyepiece view of the scope with a series of camera images. Then I put the series of images into PSE9 to stitch them all together to get a complete imag...

vasselle wrote:Bonjour
Très belles séries images.
J'aime particulièrement celle que vous avais fait sur les pollens elles sont belles.
Très bon travail,merci pour le partage.
Cordialement seb

Thank you very much vasselle for your kind comments

Absolutely superb images, both objectives. The iodine does a good job, must get some for my kit. Thanks for the post, it's very detailed and interesting.
I've a 60x - I love it too!

Hi, I've just taken this 6-picture stack (stacked with Toupview) of an onion epidermis stoma through a 60x objective... websize_tv_maxc_6stack.jpg I'm pretty pleased with the level of detail, I like the nucleus detail with it's nucleoli bottom left too. The Canon is definitely a big step up from the...

Personally I would say no I wouldn't, as well as - definitely, but instead of - no. I've an 18mp Canon mounted on my 'scope but I still see a far superior image through the binocs. But, I suspect that the lower the magnification of the camera->'scope lens not only the greater the FOV of the camera b...

Congrats, mrsonchus! I am a terrific fan of low cost LED torches...I use them all the time at my microscopy bench. Is the image of those seeds with your 4X objective? Very crisp image. Being on the other side of the pond..I'm curious about your remark: 'Heath-'...does this equate with: 'quick and d...

Very nice! I have a Celestron color filters set that I didn't use much for astronomy but I kept it having an instict it might prove useful. I'm goimg to experiment with it on my microscope, I even might try to make an adaptor for the incident LED light of the Bresser. Have a go! I bought a cheap bo...

A start anyway. Indeed! Like how the torch hangs from its strap which is placed around the binocular head! How happy are you with the colour? I think you might find that diffused light will give you a better match. Bit of tracing paper will do at a pinch. Also do you post process? Photoshop or the ...

Hi all, had a go at setting up incident-lighting to look at some larger specimens of seeds (placed in a well-slide). I've a little bottom-lighting too which gave a nice background although I think I'd have been better off with an opaque black or white card under the seeds... I got the bendy USB LED ...

Hi, add some Fuschin until it's a strong vivid red (this will take less that you think) to the 96% alcohol. Try perhaps 5ml of the alcohol to a tiny pinch of powder (on end of teaspoon handle maybe) and keep adding until its about as transparent as very rich strawberry jam.... Let it mix and settle ...

I would like a step by step recipe for make small amounts of glycerin jelly with basic Fuchsin added. I have what I need to make it I just am not sure how to dissolve the basic Fuchsin and I do not want a huge amount. Hmm - I'm not absolutely sure, I bought mine ready-made complete with Fuchsin in ...

Superb - especially the first & last!

Nice coups de créatures fascinantes. Excuses pour l'horrible Français.

Great!!! I like trying different Rheinberg filters. You can get totally different looks and if you do one you don't like, you can throw it away and move on to the nexr one. Very good image. Good DOF throughout the FOV. Thanks JimT. I think I'll definitely have a try at some more techniques involvin...

vasselle wrote:Bonjour
Très beau avec de très belle couleur
Cordialement seb

Merci beaucoup Vasselle.

Hi all, I've finally had a chance to try Rheinberg illumination. My new 'scope had no filter tray beneath the condenser - only a swing-out bullyseye lens to fill the FOV of the 4x objective with light completely. The ever helpful Brunel sent me another condenser free of charge , that does have said ...

Thanks very much John. Everything I see these days causes me to wonder how it would look under the scope. I picked up the microtome on Ebay. It works well if the driver does his job. Here is a shot of it. Looks a beauty - I'd love to try one of those. I think the preferred blade to use may often be...

Welcome back! I'm gradually learning how to collect, process and permanently mount pollen too! I've the very start of a collection - a couple of varieties of Sow Thistles (Sonchus) that are a pesky weed in the UK. I've learned an awful lot from WD's little book, all his material including everything...

Crater Eddie wrote:Looks good from here! Makes me want to give it a go.
CE

Thank you, Walter Dioni inspired me to try the onion epidermis, have a look at the website and his
contributions - you'll soon get bitten by the bug!

regards

Thank you! And, as expected, the clear nail polish is not suitable, as it caused much of the stain to migrate to the edges of the cover slip. It was quite interesting to watch. Also, the glycerin is having a similar effect, though not as pronounced and in much slower motion. CE These are really goo...

Love these pictures - good old Pine eh! I really like your exploratory approach - these are colourful and interesting pictures and I think you did well to get them. What microtome did you use?

John

Very nice indeed! If I may critique the 2nd image, I would have not cleaned the background "wholesale", but rather cloned out really objectionable, undefined objects individually and left the other stuff alone: I think that might have given it a more "natural" look. All the same, very nice images, ...

Manfred wrote:Rod,

it is also named "Etzold FCA" and is a mixture of Fuchsin, Chrysoidin and Alcianblau (sorry but I don`t know in English).
It is usable for all plant sections and gives nice results.

Manfred

This stain certainly does a good job - I wonder what the 'UK version' is sold as?

mrsonchus, you already have a nice camera for photography unless you want to become a serious photography hobbyist. A 5MP USB camera will be very good for microscope images unless you want to enlarge them to "mega size". Connectable to your lap top, can take TIFF images, and suitable for post proce...

Here are a couple of pictures of some of my first pollen mounts in Glycerin Jelly stained with Fuschin - the backgrounds have been 'everage-blurred' away to clean them up a bit as they are really pretty bitty and mucky. The Schlembergera is better known as 'Christmas Cactus' although the variety fro...

Here are several of my first rather poor attempts to stain onion epidermis with Safranin and Fast Green stains (seperately). The samples were kept for several days in 75% IPA then stained for 2 hours. They were then taken through alcohol/DIW series down to DIW only, then mounted in glycerin and seal...