Search found 3926 matches

by mrsonchus
Thu Dec 10, 2020 12:09 am
Forum: Beginner's corner
Topic: Is this normal?
Replies: 34
Views: 2654

Re: Is this normal?

Hmm the inner ring (closest to the body) looks like a tension adjuster - my Olympus BX50 has one like this. However if the ring also has a 'tab' on it it is a focus-lock to set the maximum distance the objective may be moved toward the slide - to help prevent any crashing into slides.... No-such tab...
by mrsonchus
Wed Dec 09, 2020 11:42 pm
Forum: Microscopes and optics
Topic: New Stereo - Nikon SMZ800
Replies: 17
Views: 1612

New Stereo - Nikon SMZ800

Hi all, well, having attempted an upgrade a while back now of my Amscope stereo-zoom 7.5X->45X in an attempt primarily to be able to use the photo-port at the same time as BOTH eyepieces and failing, I've tried again and yes, spent yet more money... :cry: The first 'scope had a pull-push rod to dive...
by mrsonchus
Wed Dec 09, 2020 11:36 pm
Forum: Beginner's corner
Topic: Is this normal?
Replies: 34
Views: 2654

Re: Is this normal?

IMPORTANT

Try Greg's suggestion FIRST - as if this is the way the 'scope's rack-tension is altered, the counter-turning method may well damage the mechanism...
As said the little hole (if it exists) and slightly longer shaft or extra spacing between knob and rack on one side will be obvious.
by mrsonchus
Wed Dec 09, 2020 8:20 pm
Forum: Beginner's corner
Topic: Is this normal?
Replies: 34
Views: 2654

Re: Is this normal?

Cripes! If they told you that's normal they should hang their heads in shame - they are being ridiculous.
Is it possible to return for a refund?
by mrsonchus
Mon Dec 07, 2020 9:28 am
Forum: Camera systems and imaging
Topic: Motic SMZ143 with Motic 2x SLR Projection Lens
Replies: 42
Views: 4617

Re: Motic SMZ143 with Motic 2x SLR Projection Lens

Your Motic software definitely doesn't look like T-View. Here's an example of Hayear brand T-View,
hayear branded toupview.jpeg
hayear branded toupview.jpeg (170.53 KiB) Viewed 2674 times
by mrsonchus
Mon Dec 07, 2020 1:31 am
Forum: Camera systems and imaging
Topic: Motic SMZ143 with Motic 2x SLR Projection Lens
Replies: 42
Views: 4617

Re: Motic SMZ143 with Motic 2x SLR Projection Lens

... the solid-state CMOS Moticam (a ToupCam by another name...). . Is that so, John ? My [aged] Motic cameras don’t work with the ‘Toup’ software, and my ToupCam doesn’t work with Motic software. Grateful for your advice, if I’m missing a trick ! MichaelG. Hi, yes the branded software usually has t...
by mrsonchus
Sun Dec 06, 2020 6:38 pm
Forum: Camera systems and imaging
Topic: Motic SMZ143 with Motic 2x SLR Projection Lens
Replies: 42
Views: 4617

Re: Motic SMZ143 with Motic 2x SLR Projection Lens

Hmm, the horrid blurring certainly looks like the result of movement - presumably this doesn't occur to this degree at least with the solid-state CMOS Moticam (a ToupCam by another name...). The 'turned' orientation of the camera (as you say by about 15 deg) is as far as I've experienced quite norma...
by mrsonchus
Sun Dec 06, 2020 7:25 am
Forum: My microscope
Topic: Using Flashlights
Replies: 3
Views: 747

Re: Using Flashlights

Must agree with greg above! Your setup has all the elements of what makes all of us here tick - the drive to explore and experiment!
Keep up the good work Don - great to see your adventures, your kit is an inspiration! :)
by mrsonchus
Fri Dec 04, 2020 7:20 pm
Forum: Beginner's corner
Topic: Upgrading my Laborlux K
Replies: 7
Views: 541

Re: Upgrading my Laborlux K

Small observation, I use phase quite a lot and find it's very useful indeed for intra-cellular contents (Botanical in my use) and cell-walls, often able to reveal the invisible and/or mitigate the poor differentiation of often overstained finer details of them both... The view of cellular contents o...
by mrsonchus
Wed Dec 02, 2020 5:48 pm
Forum: Beginner's corner
Topic: Spot on FoV driving me insane
Replies: 30
Views: 1291

Re: Spot on FoV driving me insane

Well done! Great when these awkward blighters finally give-up!

Onward-ho! :D
by mrsonchus
Wed Dec 02, 2020 12:15 pm
Forum: Beginner's corner
Topic: Spot on FoV driving me insane
Replies: 30
Views: 1291

Re: Spot on FoV driving me insane

Ah - you have just one mirror - the one behind the bulb. Place a piece of frosted filter or even greaseproof-paper/tracing paper over the top of the light port (on top, on the outside) to see if the mark is above (then it will still be visible) or below (where the frosted glass/paper will diffuse th...
by mrsonchus
Wed Dec 02, 2020 3:02 am
Forum: Beginner's corner
Topic: Spot on FoV driving me insane
Replies: 30
Views: 1291

Re: Spot on FoV driving me insane

Update to reply I mistakenly-gave above.... I actually meant the mirror that's below the field diaphragm not the mirror that's in the lamp-holder behing the bulb - just re-read the post and saw my careless answer - sorry. The mirror last in line (usually under) before the field-diaphragm is the one ...
by mrsonchus
Wed Dec 02, 2020 12:03 am
Forum: Beginner's corner
Topic: Spot on FoV driving me insane
Replies: 30
Views: 1291

Re: Spot on FoV driving me insane

Try the mirror before you start to dimantle - this really did solve this problem for me... The mirror next to the bulb correct? Yes, the mirror that reflects the light into the condenser. My system was showing an annoying blue mark that didn't move whatever element of the illumination and image tra...
by mrsonchus
Tue Dec 01, 2020 10:42 pm
Forum: Beginner's corner
Topic: Spot on FoV driving me insane
Replies: 30
Views: 1291

Re: Spot on FoV driving me insane

Try the mirror before you start to dimantle - this really did solve this problem for me...
by mrsonchus
Tue Dec 01, 2020 9:11 pm
Forum: Beginner's corner
Topic: Spot on FoV driving me insane
Replies: 30
Views: 1291

Re: Spot on FoV driving me insane

Sounds very similar to such a frustrating problem I had a while back now with my L Orthoplan - it was a mark on the mirror I eventually discovered....
by mrsonchus
Tue Dec 01, 2020 12:03 pm
Forum: Identification help
Topic: What is this? i have no idea at all
Replies: 10
Views: 606

Re: What is this? i have no idea at all

Argghhhh - I'm off to the hills! :o
by mrsonchus
Tue Dec 01, 2020 8:22 am
Forum: Identification help
Topic: What is this? i have no idea at all
Replies: 10
Views: 606

Re: What is this? i have no idea at all

Hmm, doesn't look like a trichome to me. It does however look very much like a feather - not a main plumage feather but a downy 'under-layer' type. These are common on almost all birds but rarely seen as they don't shed -regrow as the large feathers do. Very commonly shed by parrots as they preen fo...
by mrsonchus
Sun Nov 29, 2020 7:30 pm
Forum: Pictures and Videos
Topic: Help with improving image
Replies: 17
Views: 1001

Re: Help with improving image

Also stacking can have too-many images if the subject is transparent/translucent and overlapping points of focus are included in the stack - I see this a lot with plant-section images also. As advised above, keep it simple and perfect technique first.
by mrsonchus
Sun Nov 29, 2020 10:47 am
Forum: Pictures and Videos
Topic: Help with improving image
Replies: 17
Views: 1001

Re: Help with improving image

Not a horrid image by any measure, but easily improved. As you are you have a somewhat chaotic mix of technique and consequentially results - no problem to improve that. Personally, I'd say keep it simple and refine the basics first. So, forget the polarisation, cropping, oblique, magnification etc ...
by mrsonchus
Sat Nov 28, 2020 10:27 am
Forum: Microscopes and optics
Topic: LWD objective on 0.18 cover slip slide.
Replies: 15
Views: 782

Re: LWD objective on 0.18 cover slip slide.

Easily-done! I suspect that one's for inverted microscopy - hence the LWD and the thick cover-slip (petri dish bottom)... Personally I'd re-list that one and crack on with looking for the correct one - you'll only ever be compromising with this one, better to start with the right one/s. Just a case ...
by mrsonchus
Sat Nov 28, 2020 10:19 am
Forum: Camera systems and imaging
Topic: Calibration problems Moticam
Replies: 6
Views: 645

Re: Calibration problems Moticam

I'd just image something with the 40x - add measurement line or scale-bar, then use the line (which like the scale-bar will be of a known length) or scale-bar to calibrate the 100x.
This will certainly give you high accuracy - more than enough for your context I'm certain.
by mrsonchus
Thu Nov 26, 2020 1:20 pm
Forum: Beginner's corner
Topic: STAINS.. Sooo many these days
Replies: 7
Views: 689

Re: STAINS.. Sooo many these days

Agree with Bob, start perhaps with the simple 'gram-stain' for bacteria - 2-stain method available cheaply in a kit.

Cotton-blue - for fungi perhaps, again easy.
by mrsonchus
Thu Nov 26, 2020 12:06 am
Forum: Identification help
Topic: Diatom ID Please
Replies: 24
Views: 1109

Re: Diatom ID Please

Almost forgot, a stack and a crop of it, https://lh3.googleusercontent.com/pw/ACtC-3fjp2w9yulQ2e-KmeL7wUzX5UDo9-z7hnUjl9DYdNaQozrUM6FXXnHur4rn0DCML6vMKCwlcRr3pBIlvACfRrVxtTRbVxVRe64dkzyqnlapXf_RB8snbHCS6_1sJL6tRIaflGZaPg7ek8nfq_o8co5_=w1403-h937-no?authuser=0 Cropped, https://lh3.googleusercontent.c...
by mrsonchus
Wed Nov 25, 2020 10:42 pm
Forum: Identification help
Topic: Diatom ID Please
Replies: 24
Views: 1109

Re: Diatom ID Please

Thanks for the details John B. Impressive ! I am practicing diatom selection, and the only messy case that I dared to challenge so far was to finish the mounting of a diatoms on a coverslip, when the coverslip suddenly flipped and fell on a carpet and, according to the Buttered Toast Free Fall theo...
by mrsonchus
Wed Nov 25, 2020 10:39 pm
Forum: Identification help
Topic: Diatom ID Please
Replies: 24
Views: 1109

Re: Diatom ID Please

Hi again. The mount is definitely improving, the diatoms are now just visible with 'normal' BF! Here are a few images just taken I used both an IF550 filter and a slight touch of oblique to give a little relief - which I think has added detail. Also I was able to use my 60x (which I only have in BF ...
by mrsonchus
Wed Nov 25, 2020 8:29 pm
Forum: Identification help
Topic: Diatom ID Please
Replies: 24
Views: 1109

Re: Diatom ID Please

. I can't either at the moment but I could before. Wanted to ask what you used to manipulate them? Hi 75' - all I did was pick them up as it were with a very-fine paintbrush - which could of course easily be refined but was O.K. for this 'proof of concept' if you like. (something I habitually do be...
by mrsonchus
Wed Nov 25, 2020 6:41 pm
Forum: Identification help
Topic: Diatom ID Please
Replies: 24
Views: 1109

Re: Diatom ID Please

Fixing-it now chaps!

How's that now?
by mrsonchus
Wed Nov 25, 2020 5:36 pm
Forum: Identification help
Topic: Diatom ID Please
Replies: 24
Views: 1109

Diatoms Reborn!

Update! Managed to remove pieces of coverslip from shards of broken original slide! A good number of diatoms and pieces were salvageable after a short application of 'Histoclear II'.... I re-mounted them in 'Histomount' with coverslip of course. The originals were mounted I think in a higher RI moun...
by mrsonchus
Wed Nov 25, 2020 10:23 am
Forum: Pictures and Videos
Topic: Leaf casts
Replies: 10
Views: 631

Re: Leaf casts

Very nice - they really have come-out well!
A great method for quick documentation of surface characters, and with almost fresh morphology too.
by mrsonchus
Wed Nov 25, 2020 10:20 am
Forum: Camera systems and imaging
Topic: Imaging Diatoms RBGE
Replies: 4
Views: 686

Re: Imaging Diatoms RBGE

Very good links 75' - the last one especially useful for general management of data from all of our microscopic observations. The coded fields are very practical I think also. Also a timely reminder to build flexibility into any database approach as taxonomy now is an ever-changing landscape for cer...