Search found 1108 matches

by Charles
Tue Mar 06, 2018 11:33 pm
Forum: Specimens, samples and slides
Topic: Doing Diatoms a Different Way
Replies: 174
Views: 31305

Re: Doing Diatoms a Different Way

I have quite a few diatoms which are large (like the Isthmia in the last video), have projections (like Kittonia and Stephanopyxis) or are delicate and I don't want to crush them. I remember Rod had those long strands of Ellerbeckia which were crushed by the mountant and cover glass.
by Charles
Tue Mar 06, 2018 11:20 pm
Forum: Beginner's corner
Topic: Looking for first microscope
Replies: 86
Views: 8511

Re: Looking for first microscope

Here is your best option in a used microscope which will out perform any of those Chinese made scopes. This is a research grade Zeiss WL trinocular phase microscope at a very good price. https://www.ebay.com/itm/Carl-ZeissTrinocular-Phase-Contrast-Microscope/323122931559?hash=item4b3ba0a767:g:mAkAAO...
by Charles
Tue Mar 06, 2018 11:16 pm
Forum: For forum members who want to buy and sell equipment
Topic: Zeiss WL Phase Trinocular on Ebay
Replies: 3
Views: 1045

Zeiss WL Phase Trinocular on Ebay

Here is a Zeiss WL Phase trinocular microscope on ebay, which looks like it's in very good condition. This is a research grade scope with lots of options and great upgrade possibilities all for a very nice price. Not mine but I would definitely buy it if I didn't already have too many. https://www.e...
by Charles
Mon Mar 05, 2018 10:12 pm
Forum: Pictures and Videos
Topic: A few stills from the seawater slide
Replies: 16
Views: 1183

Re: A few stills from the seawater slide

Great pictures!

The diatom in the first picture is Licmophora.
Pictures 2-5 are all Achnanthes.

Good to see you back posting pictures!
by Charles
Mon Mar 05, 2018 10:08 pm
Forum: Beginner's corner
Topic: Zeiss KF2
Replies: 17
Views: 3109

Re: Zeiss KF2

I agree with 75RR. This is the very basic, low end model, but it should be able to do what you want. The condenser is fixed to the stage although it does have a iris diaphragm and a flip in filter holder. I also agree the price is too high. You should be able to get it for about half the asking price.
by Charles
Mon Mar 05, 2018 9:37 pm
Forum: Specimens, samples and slides
Topic: Doing Diatoms a Different Way
Replies: 174
Views: 31305

Re: Doing Diatoms a Different Way

Here is the aluminum pie tin, cardboard backing and hypo needles with clipped ends.
Pie Tin and Needles.jpg
Pie Tin and Needles.jpg (86.42 KiB) Viewed 3682 times
And the resulting aluminum spacers.
Spacer discs.jpg
Spacer discs.jpg (81.04 KiB) Viewed 3681 times
And here they are on the edge of a 18mm dia coverslip to show size in relationship to coverslip.
Spacer discs along coverslip.jpg
Spacer discs along coverslip.jpg (58.51 KiB) Viewed 3680 times
by Charles
Mon Mar 05, 2018 9:06 pm
Forum: Specimens, samples and slides
Topic: Doing Diatoms a Different Way
Replies: 174
Views: 31305

Re: Doing Diatoms a Different Way

They are not washer shaped but more of a small plug of circular aluminum to put at the corners of the coverslip, like posts holding up a roof. If they were washer shaped the mountant fumes and bubbles would be trapped instead of escaping out from under the coverslip when heated. The mounting medium ...
by Charles
Mon Mar 05, 2018 7:54 pm
Forum: Specimens, samples and slides
Topic: Doing Diatoms a Different Way
Replies: 174
Views: 31305

Re: Doing Diatoms a Different Way

Working on it Kurt and will post results. In conjunction, I'm trying to figure the best way to raise the coverslip on large or fragile diatoms, so they won't be crushed. I've heard of rings being used as well as using pieces of broken coverslips on the corners to keep the coverslip raise. I've come ...
by Charles
Fri Mar 02, 2018 11:56 am
Forum: Collecting microscopes and slides
Topic: Leitz microscope.
Replies: 2
Views: 1571

Re: Leitz microscope.

Good looking scope! Got to love the old brass and black scopes.
by Charles
Fri Mar 02, 2018 11:54 am
Forum: Specimens, samples and slides
Topic: Doing Diatoms a Different Way
Replies: 174
Views: 31305

Re: Doing Diatoms a Different Way

Thank you Doron.
by Charles
Fri Mar 02, 2018 11:52 am
Forum: Beginner's corner
Topic: Mounting medium question
Replies: 17
Views: 2128

Re: Mounting medium question

How about Canada Balsam? It's been used for ages for most permanent mounts
Another option is CytoSeal. I have never used it but it is inexpensive:
https://www.ebay.com/itm/NC-5988-CytoSe ... 1438.l2649
by Charles
Thu Mar 01, 2018 5:07 pm
Forum: Specimens, samples and slides
Topic: Doing Diatoms a Different Way
Replies: 174
Views: 31305

Re: Doing Diatoms a Different Way

You're welcome JWW. Kurt, it's actually much better and smoother when I don't have to squint down one tube. Because when I swing the photo port in, it blacks out the left eyepiece, which makes it hard to judge depth of view and targeting. Yes, I have been making some permanent type slides too. Still...
by Charles
Wed Feb 28, 2018 7:07 pm
Forum: Specimens, samples and slides
Topic: Doing Diatoms a Different Way
Replies: 174
Views: 31305

Re: Doing Diatoms a Different Way

Did another video of selecting diatoms off a strew slide and moving to a storage slide. This sample is from Puget Sound, WA:
https://youtu.be/g-F0Id_m3Ys
by Charles
Tue Feb 27, 2018 10:23 pm
Forum: Beginner's corner
Topic: Word to the Wise: Lessons Learned
Replies: 18
Views: 3525

Re: Word to the Wise: Lessons Learned

Doing Diatoms is here: viewtopic.php?f=10&t=3036
And Doing Diatoms a Different Way is here: viewtopic.php?f=10&t=3036
by Charles
Wed Feb 14, 2018 12:32 pm
Forum: Introduce yourself
Topic: Greetings from Cecil County Maryland
Replies: 8
Views: 2166

Re: Greetings from Cecil County Maryland

That is similar to my B&L discussed here:
viewtopic.php?f=11&t=1849
I believe these were top of the line research microscopes at the time for B&L
by Charles
Mon Feb 12, 2018 6:03 pm
Forum: Pictures and Videos
Topic: It’s all witchcraft to me
Replies: 7
Views: 680

Re: It’s all witchcraft to me

They are all probably usable, depending on how much time/money you want to spend fixing them up and finding replacement parts. The most complete one is the Zeiss 405 IM, the inverted microscope. You just need some objectives and a 12V power supply for the 12V lamp. You might be able to use the power...
by Charles
Mon Feb 12, 2018 5:10 pm
Forum: Pictures and Videos
Topic: It’s all witchcraft to me
Replies: 7
Views: 680

Re: It’s all witchcraft to me

There is no cosistant numbering so I will assume them fall in order. #3-9: is a Reichert photo adapter #17: which are the two large round objects are Zeiss lamphouses used for HBO or high pressure mercury lights. #18-19 is a 6V Zeiss lamp which can be used for Zeiss Standard microscopes #20&22: Zeis...
by Charles
Fri Feb 09, 2018 4:28 pm
Forum: Beginner's corner
Topic: Help to buy a microscope
Replies: 31
Views: 3311

Re: Help to buy a microscope

You should ask about all the functions such as the coarse and fine focus controls, the condenser controls and condenser iris control. Make sure the objective turret turns smoothly and clicks into each objective. have him make sure all optics (eye pieces, objectives, condenser) are clear and not defe...
by Charles
Fri Feb 09, 2018 11:54 am
Forum: Beginner's corner
Topic: Help to buy a microscope
Replies: 31
Views: 3311

Re: Help to buy a microscope

Yes, that is a condenser. I don't know how I missed that picture. And yes, you can get a clip on mechanical stage.

I think your son will get a lot of enjoyment it that microscope.
by Charles
Fri Feb 09, 2018 12:28 am
Forum: Beginner's corner
Topic: Help to buy a microscope
Replies: 31
Views: 3311

Re: Help to buy a microscope

Hi Katerina, Of the two you list, I would go with the second LW. Only reservations would be that the stage only has stage clips, which means you need to move the slide by hand to observe different parts of your specimen. A mechanical stage allows you to move the slide in the x-y direction with contr...
by Charles
Wed Feb 07, 2018 3:54 pm
Forum: Beginner's corner
Topic: New to Microscopy
Replies: 6
Views: 1189

Re: New to Microscopy

Hi Rainwoman,

You might try Greg McHone's site:
http://earth2geologists.net/Microscopes/
by Charles
Tue Feb 06, 2018 11:36 pm
Forum: Introduce yourself
Topic: New member from Pittsburgh
Replies: 59
Views: 7263

Re: New member from Pittsburgh

Some microscopes came out with 3 colour coded brushed chrome objectives and a gold plated 20X. The code they used at the time was green/10X, blue/20X, yellow/40X( 43X in this case) and red/100X(90X in this case). About the same time or a little after, they made a 10X .30 apo and a 43X .80 apo for t...
by Charles
Mon Feb 05, 2018 4:45 pm
Forum: Beginner's corner
Topic: Is this normal for objective lenses?
Replies: 7
Views: 1018

Re: Is this normal for objective lenses?

Are using oil immersion with the 100X 1.25NA objective?
by Charles
Mon Feb 05, 2018 12:32 am
Forum: Introduce yourself
Topic: Greetings from Cecil County Maryland
Replies: 8
Views: 2166

Re: Greetings from Cecil County Maryland

Welcome Mike,

Can you post a picture of your microscope? It would help with identification.
by Charles
Thu Feb 01, 2018 9:54 pm
Forum: Beginner's corner
Topic: DIC microscopy
Replies: 5
Views: 916

Re: DIC microscopy

DIC: Differential interference contrast microscopy, also known as Nomarski interference contrast.
by Charles
Sun Jan 28, 2018 5:32 pm
Forum: Microscopes and optics
Topic: Is a Zeiss C8x eyepiece useful for afocal or focal photomicrography?
Replies: 4
Views: 667

Re: Is a Zeiss C8x eyepiece useful for afocal or focal photomicrography?

Here is a link to the older Zeiss Optical Systems catalog. In the eyepiece section it states the C eyepiece is only suitable for low NA objectives.
http://www.science-info.net/docs/zeiss/
by Charles
Sun Jan 07, 2018 3:55 pm
Forum: My microscope
Topic: Nikon S-Kt with trinocular head? *edit* with better pics
Replies: 8
Views: 2173

Re: Nikon S-Kt with trinocular head?

Can't really tell what you have with just a downward view of your scope. A side and/or front view would be better for identification. Yes, you can search for a bulb with a part number but it may not have one. but the bulb should have Volts and Watts on it. Then you can search by Volts, Watts, type o...
by Charles
Sun Jan 07, 2018 3:50 pm
Forum: Beginner's corner
Topic: New microscope troubleshooting
Replies: 5
Views: 1049

Re: New microscope troubleshooting

Hello Rob, The microscope you linked to comes with a 10X, 20X, 40X and 100X Plan Phase objectives and 5X, 10X, 40X and 100X Plan objectives with a phase condenser. It does not mention a field iris for Kohler. Considering the price and features of your microscope, I would assume your objectives are p...
by Charles
Thu Dec 21, 2017 2:36 pm
Forum: Introduce yourself
Topic: New/beginner hobbyist
Replies: 29
Views: 5802

Re: New/beginner hobbyist

A very nice buy. It's good you got the 327 with full Kohler

Did you get this new or used? Generally these go for around $450-$500 new.